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수삼음경의 락혈 침자가 백서의 혈위 조직내 nNOS. NO와 조직 및 혈장 Norepinephrine의 변화에 미치는 영향
신욱 ( Wook Shin ),이유미 ( Yumi Lee ),이경인 ( Kyoungin Lee ),최동희 ( Donghee Choi ),김미래 ( Mirae Kim ),나창수 ( Changsu Na ),김선민 ( Sunmin Kim ),표병식 ( Byoungsik Pyo ),윤대환 ( Daehwan Youn ) 대한경락경혈학회 2016 Korean Journal of Acupuncture Vol.33 No.2
Objectives : A previous study demonstrated that the connecting points of three yang meridians attenuated changes of nNOS, and Norepinephrine(NE) in rats. The current study investigated the changes in nNOS, NO and NE upon the needle insertion at varying depths at the connecting point of three yin meridians of the hand. Methods : Needles were inserted into rats, on both left and right sides of the connecting point, including the LU7, HT5 and PC6 acupoints which are three yin meridians of the hand. After insertion, needles were retained for five minutes. Each acupuncture groups were treated acupuncture at each acupoint and at the depths of superficial, middle and deep layer. After the retention, blood was drawn via cardiac puncture, and tissues of each point near meridian vessel was extracted to examine the changes in the expression of nNOS, NO and NE. Results : Compared with the superficial layer group, nNOS production significantly decreased in the middle and deep layer at LU7 acupoint group and in the deep layer at HT5, PC6 acupoint group. The tissue NE decreased in the deep layer on PC6 acupoint and the plasma NE increased at the middle layer at LU7 acupoint group but decreased at the deep layer on at LU7 acupoint group. Conclusions : Acupuncture at connecting points of three yin meridians of the hand can regulate the activities of nNOS, and NE.
김영선 ( Youngsun Kim ),최동희 ( Donghee Choi ),장호선 ( Hosun Jang ),나창수 ( Changsu Na ),최태진 ( Taejin Choi ),황문현 ( Moonhyeon Hwang ),조주현 ( Joohyun Cho ),이경인 ( Kyoungin Lee ),김선민 ( Sunmin Kim ),표병식 ( Byoungsik 경락경혈학회 2013 Korean Journal of Acupuncture Vol.30 No.4
Objectives: To observe the changes in the expression of neurotransmitters NO and enzymes that create NO, such as nNOS, iNOS and eNOS, upon the needle insertion on river point, one of the five transport points of three yang meridians on the forefoot. Methods: Based on rats, needle was inserted on both sides of LI5, SI5 and TE6, which are river points of three yang meridians on the forefoot, and were retained for five minutes. After the retention, blood was drawn via cardiac puncture and tissues from each point around meridian vessels were extracted to be examined on the changes of the expression of NO, as well as of nNOS, iNOS and eNOS. Results: In terms of the effect on NO creation in tissues, none of the experimental groups showed any significant change compared to the Normal group. In terms of the effect on expression of nNOS within tissues, LI5 and SI5 showed significant increase based on the results of immunohistochemistry. In iNOS within tissues, LI5 and SI5 showed significant increase based on the results of immunohistochemistry. In eNOS within tissues, SI5 showed significant increase based on the results of immunohistochemistry. Conclusions: The effect on the function of NO, nNOS, iNOS and eNOS of needle insertion on the river points of three yang meridians on the forefoot could be observed, and based on this study, it is considered that the effect of needle stimulation on the changes of nervous system could be found out through additional research.