두부외상후 만성 기질성 정신장애 환자에서 Tc-99m HMPAO Brain SPECT 분석

이강욱,이종진,송민호,강민희,지익성,신영태,노흥규 ( Kang Wook Lee,Jong Jin Lee,Min Ho Shong,Min Hee Kang,Ick Sung Ghi,Young Tai Shin,Heung Kyu Ro ) 대한핵의학회 1994 핵의학 분자영상 Vol.28 No.3

It is well known that Tc-99m HMPAO brain SPECT can reflect the functional lesions better than X-ray computerized tomography(CT) and magnetic resonance imaging(MRI) in the cerebral disorders. In order to evaluate the clinical utilities of Tc-99m HMPAO brain SPECT in patients with post-traumatic chronic organic mental disorder(OMD). We included 28 patients diagnosed as OMD in department of psychiatry after traumatic head injury. And we compared the results of Tc-99m HMPAO SPECT with those of MRI, EEG and MINI mental status examination(MMSE). The results were as follows 1) All patients diagnosed as OMD showed diffuse or focal decreased cerebral perfusion on Tc-99m HMPAO SPECT. 2) Most frequent lession on brain Tc-99m HMPAO SPECT was decreased perfusion on both frontal lobe. And most frequent lesion on brain Tc-99m HMPAO SPECT was decreased perfusion on both frontal lobe. And most frequent lesion on brain Tc-99m HMPAO SPECT showing normal brain MRI result was also decreased both frontal perfusion. 3) Eight of 28 patients showed focal brain MRI lesions(4 small frontal hygroma, 3small cerebral infarction and 1 cerebellar encephalomalacia) which were not detected in brain Tc-99m HMPAO SPECT. 4) The patients showing less than 20 points on MMSE disclosed abnormal results of EEG more frequently than those disclosing more than 20points. In conclusion, we think that Tc-99m HMPAO brain SPECT is sensitive method to detect functional lesions of the brains in patients with chronic post-traumatic organic mental disoder.

유방암 치료에 대한 한의 임상 가이드라인 개발을 위한 기초연구

이강욱,유화승,이진선,이남헌,Lee, Kang-Wook,Yoo, Hwa-Seung,Lee, Jin-Sun,Lee, Nam-Hun 대한한방부인과학회 2016 大韓韓方婦人科學會誌 Vol.29 No.2

Objectives : The purpose of this study was to present the clinical practice guidelines for breast cancer through the analysis of existing clinical practice guidelines in Western and Chinese medicine. Methods : The literature search was performed regarding conventional and integrative medical treatment of breast cancer using Google Scholar, KIOM OASIS, PUBMED and Jisan library of Daejeon university. Results : Breast cancer is the fifth most common cancer in Korea. Recent studies revealed that combining conventional and integrative medical treatment can reduce the sequela of surgical operation and improve survival rate. However there isn’t still a systemic clinical guideline for breast cancer in korean medicine. This study will be a preliminary study to establish clinical practice guidelines of Korean Medicine for breast cancer.Conclusions : Further objective and systemic clinical studies related to Korean Medicine are needed to develope more advanced clinical practice guidelines of breast cancer.

김치유산균용 발현벡터 pSJE6c 개발과 이를 이용한 외래 유전자 발현

이강욱,박지영,이지연,이황아,백창운,조현덕,김주연,권건희,천지연,김정환,Lee, Kang-Wook,Park, Ji-Yeong,Lee, Ji-Yeon,Lee, Hwang-A,Baek, Chang-Un,Jo, Hyeon-Deok,Kim, Joo-Yeon,Kwon, Gun-Hee,Chun, Ji_Yeon,Kim, Jeong-Hwan 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.25 No.4

본 연구실에서 개발한, 김치에서 분리한 Leu. mesenteroides SY2 유래 pFMBL1 을 바탕으로 구축한 셔틀벡터인, pSJE[7]를 외래유전자 발현에 적합하게 개량한 발현벡터를 구축하였다. Lactococcus lactis LM0230에서 분리한 프로모터 P6C를 pSJE에 도입하였다. P6C 염기서열을 지닌 oligonucleotide 쌍을 따로 제조한 후 annealing을 통해 짧은 DNA 단편을 얻어서 제한효소 처리후 pSJE에 도입하여 pSJE6c를 구축하였다. PSJE6c 효능 검증을 위해서 외래 유전자인 aga와 lacZ를 각각 pSJE6c에 도입하였다. P6C 프로모터와 비교를 위해 고유 프로모터를 지닌 유전자들도 각각 pSJE에 도입하였다. 재조합 plasmid들을 electroporation 방법으로 Lactobacillus brevis 2.14 균주에 도입하고 재조합균주들의 생육곡선과 효소역가 그리고 slot blot으로 전사체 농도를 측정하였다. 결과를 보면 PSJE6c에 클로닝 된 유전자들이 pSJE상의 유전자보다 효소역가들이 약 1.5배에서 2배 정도로 높았다. 전사체 농도 측정 결과도 pSJE6c 들에서 더 많은 전사체가 생성됨을 보여주었다. 이상 결과들은 효율적인 발현벡터들의 사용을 통해서 김치유산균에서 외래유전자 발현 효율을 높일 수 있음을 보여준다. Development of expression vectors is important for the basic and applied researches on kimchi LAB (lactic acid bacteria). An expression vector, pSJE6c was constructed by inserting P6C promoter sequence from Lactococcus lactis into pSJE, a shuttle vector for E. coli and Leuconostoc species. To test the efficiency of pSJE6c, aga ($\alpha$-galactosidase) and lacZ ($\beta$-galactosidase) genes were expressed in Lactobacillus brevis 2.14. Compared to the pSJE, expression levels of both genes were increased, indicating P6C promoter was better than indigenous promoters. Enzyme activities of L. brevis cells harboring pSJE6caga (pSJE6c with aga) or pSJE6Z (pSJE6c with lacZ) were 1.5-2 fold higher than those with pSJEaga (pSJE with aga) or pSJEZ (pSJE with lacZ). More RNA transcripts were detected in cells harboring pSJE6c based recombinant plasmid. The results indicated that heterologous gene expressions in kimchi LAB could be improved significantly by use of efficient expression vectors.

3-D Hetero-Integration Technologies for Multifunctional Convergence Systems

이강욱,Lee, Kang-Wook The Korean Microelectronics and Packaging Society 2015 마이크로전자 및 패키징학회지 Vol.22 No.2

Since CMOS device scaling has stalled, three-dimensional (3-D) integration allows extending Moore's law to ever high density, higher functionality, higher performance, and more diversed materials and devices to be integrated with lower cost. 3-D integration has many benefits such as increased multi-functionality, increased performance, increased data bandwidth, reduced power, small form factor, reduced packaging volume, because it vertically stacks multiple materials, technologies, and functional components such as processor, memory, sensors, logic, analog, and power ICs into one stacked chip. Anticipated applications start with memory, handheld devices, and high-performance computers and especially extend to multifunctional convengence systems such as cloud networking for internet of things, exascale computing for big data server, electrical vehicle system for future automotive, radioactivity safety system, energy harvesting system and, wireless implantable medical system by flexible heterogeneous integrations involving CMOS, MEMS, sensors and photonic circuits. However, heterogeneous integration of different functional devices has many technical challenges owing to various types of size, thickness, and substrate of different functional devices, because they were fabricated by different technologies. This paper describes new 3-D heterogeneous integration technologies of chip self-assembling stacking and 3-D heterogeneous opto-electronics integration, backside TSV fabrication developed by Tohoku University for multifunctional convergence systems. The paper introduce a high speed sensing, highly parallel processing image sensor system comprising a 3-D stacked image sensor with extremely fast signal sensing and processing speed and a 3-D stacked microprocessor with a self-test and self-repair function for autonomous driving assist fabricated by 3-D heterogeneous integration technologies.

Guinea pig의 갑상선기능(甲狀腺機能)이 부신(副腎), 뇌하수체(腦下垂體) 및 생식선(生殖腺)에 미치는 영향(影響) I. Guinea pig의 갑상선(甲狀腺)이 부신(副腎)에 미치는 영향(影響)

이강욱,이규승,정영채,Lee, Kang Wook,Lee, Kyu Sung,Cheong, Young Chai 대한수의학회 1968 大韓獸醫學會誌 Vol.8 No.2

The mature guinea pigs were grouped as indicated in the table 1. Radio-active iodine(I-131)in dose of 4.5mci, was administered to the experimental groups. The animals were killed for examination in 1, 7, 14, 28, 42, and 55 days after the administration the radio-active iodine. The thyroid and adrenal glands were observed histologically. The results obtained were as follows; 1. One day after the administration, thyroid epithelial cells were abnormally enlarged. After seven days, specimens taken from the middle of the thyroid showed that the follicles and epithelial cells were changing to fibrous tissue, however, some follicles still remained in the verge of the thyroid. Follicles were not observed after fourteen days. After twenty-eight days, the follicles had all changed to fibrous tissue, and had lost their function. 2. The size of the zonas gromerulosa of adrenal cortex epually, in both male and female, showed slight fluctuation in size with no tendency to be changed. 3. Among the zones of the adrenal glands, zona fasciculata showed marked changes. Zona fasciculata was atrophied in Process of time. In females, it was atrophied significantly(P<0.05) after fourteen days, and highly significant (P<0.01) in twenty-eight and fifty-six days after the administration of radioactive iodine. In males, it also decreased significantly(P<0.05) in seventy-eight days and highly significant(P<0.01) in forty-two and fifty-six days after the administration. 4. The size of the Zona reticularis of adrenal cortex in the females increased significantly (P<0.05) in twenty-eight days after the administration. In males, it showed slight fluctuation until twenty-eight days, but it increased significantly(P<0.05) in forty-two and fifty-six days after the administration. 5. The size of the adrenal medulla increased significantly(P<0.05) in twenty-eight and forty-two days in females. It was increased significantly(P<0.05) in fourth-two days and high significantly(P<0.01) in fifty-six days after the administration.

임신 Rat에 있어서 방사성인(³²P)의 흡수 분포에 관한 연구

이강욱,정영채,이홍식,남치주,Lee, Kang Wook,Chung, Yung Chai,Lee, Heung Shik,Nam, Tchi Chou 대한수의학회 1969 大韓獸醫學會誌 Vol.9 No.2

The distribution of radiophosphorus ($^{32}P$) was observed in three groups of female Albino tat consisted of early pregnancy group (10 days of pregnancy), late pregnancy group (18 days of pregnancy) and nonpregnanted control group. The results obtained were as follows: 1. The various sites arranged in the descending of absorption rate of $^{32}P$ were femur, mandible, liver, spleen, ovary, fetus (only from early pregnancy group) and kidney in both groups of non-pregnanted and early pregnancy and femur, mandible, fetus, liver, ovary, spleen and kidney in the late pregnancy group. 2. The significant difference (P<0.05) in the absorption rate was found between femur and mandible in the early pregnancy group. 3. The absorption rate of fetus from late pregnancy group was significantly (P<0.01) higher than that of fetus from the early pregnancy group. 4. The high absorption rate was observed with femur and mandible in both groups of non-pregnanted and early pregnancy. 5. The significantly (P<0.01) high absorption rate was observed with ovary and fetus from the late pregnancy group.

진료역량 중심의 기본의학교육 학습성과

이강욱,Lee, Kang Wook 연세대학교 의과대학 2016 의학교육논단 Vol.18 No.3

Outcome-based learning is a global trend in medical education. The Korean Association of Medical Colleges (KAMC) has been developing learning objectives for basic medical education (BME) in Korea. In 2012, KAMC published the 1st edition of "Learning outcomes of basic medical education: Clinical competency-centered" in order to promote outcome-based medical education. KAMC has recently revised and updated the learning outcomes of basic medical education in the clinical competency-centered 2nd edition to reflect the suggestions of all medical schools in Korea and improve application of the published learning outcomes for BME in the field of medical education. KAMC has been making efforts to integrate clinical competency-centered learning outcomes with scientific concepts and principle-centered learning outcomes in addition to basic clinical skills and performance in BME.

한인진(韓茵蔯), 울금(鬱金), 산사(山査), 단삼(丹蔘) 발효복합추출물이 RAW264.7 및 HUVEC 세포에서 나타나는 이상지질혈증 관련 염증인자 발현 및 항산화에 미치는 영향

이강욱,조현경,유호룡,설인찬,김윤식,Lee, Kang-wook,Cho, Hyun-kyoung,Yoo, Ho-ryong,Seol, In-chan,Kim, Yoon-sik 대한한방내과학회 2018 大韓韓方內科學會誌 Vol.39 No.4

Objectives: To investigate the effect of fermented extract of Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix (FMH) on anti-inflammation associated with dyslipidemia and anti-oxidation in RAW264.7 and HUVEC cells. Methods: The total polyphenols, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity, and cytotoxicity of FMH were measured. RAW264.7 cells treated with FMH were tested for production of NO, and for cytokine and LTB4 levels and HUVEC cells treated with FMH were examined for production of cDNA of genes related to inflammation. Results: 1. FMH contained polyphenols and flavonoids. The DPPH and ABTS radical scavenging activity of FMH increased in a concentration-dependent manner. 2. FMH treatment inhibited the production of nitric oxide (NO), cytokines, and LTB4 in RAW264.7 cell when compared to the untreated control group. 3. FMH decreased the transcription of pro-inflammatory genes, whereas it increased transcription of anti-inflammatory genes, in HUVEC cells. Conclusion: FMH is effective as an antioxidant and for treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke, and other cardiocerebrovascular diseases.

입체복합공간 개발사업의 프로그램 성과관리 체계 구축

이강욱,홍화욱,박희대,한승헌,Lee, Kang-Wook,Hong, Hwa-Uk,Park, Hee-Dae,Han, Seung-Heon 한국건설관리학회 2011 한국건설관리학회 논문집 Vol.12 No.1

도시재생사업의 시장 규모는 꾸준히 증가하고 있으며, 도시재생 사업에서 큰 비중을 차지하는 입체복합공간 개발사업 역시 증가하는 추세를 보이고 있다. 하지만 기존의 도시재생 관련 연구는 국내외 사례의 비교분석에 기반한 시사점 및 제도의 개선방안 도출이 주를 이루어왔으며, 사업성패여부와 관련 깊은 사업관리 분야의 연구, 특히 사업관리의 기초를 제공하는 성과관리에 대한 연구는 상대적으로 미비한 실정이다. 본 연구는 입체복합공간 개발사업의 성공적인 추진을 위해 공공발주자 관점의 프로그램 성과관리 체계를 구축하는데 목적을 두고 진행되었다. 이를 위해 기존 문헌분석, 전문가 인터뷰 등을 실시하였으며, 입체복합공간 개발사업의 시설물 유형별 성과지표 및 리스크 연계 성과지표를 도출하고, 프로그램 성과점수 통합방법을 제안하였다. 본 연구에서 제안한 성과관리 체계는 기본계획 수립단계에서부터 유지관리단계까지 전 생애주기를 고려하고 있으며, 시설물 유형별 성과를 실시간으로 파악 및 평가가 가능한 구조를 갖추고 있다. 또한 리스크 연계 성과관리 체계는 주요 리스크요인별 대응주체 및 대응방안을 제공함으로써 리스크가 높은 성과지표의 지속적인 추적 및 통제를 가능하게 하였다. 본 연구는 도시재생사업의 전 생애주기에 걸친 성과관리 기술 향상을 통해 해당 분야의 효율성 제고에 기여할 것으로 기대되며, 향후 데이터웨어하우스 기반의 웹기반 성과관리시스템의 기본구조로 활용될 것이다. The market volume of urban regeneration projects has steadily increased, thereby mixed-use development also shows significant market growth. However, previous researches on urban regeneration mainly deduced suggestions and plans for institution improvement based on comparison between projects implemented the inside and outside of the country. Whereas, researches on project management closely related to project success were limited, particularly in performance management. Accordingly, this research aims at developing a program performance management framework for public clients so as to succeed mixed-use development projects. Through extensive literature review and expert interview, this research developed performance indices for diverse facilities, risk management framework and integrating method of program performance score. The proposed framework is able to consider total life cycle from basic planning to maintenance phase and to check real-time performance level. Moreover, risk management framework can periodically assess and control the level of inherent risks within performance indices. The results can contribute to improve existing performance management practices and be basis for a web-based system developed in future.

삼차원적층형 집적회로 구현을 위한 자기조직화정합기술을 이용한 고속.고정밀 접합기술

이강욱,Lee, Kang-Wook 대한용접접합학회 2011 대한용접·접합학회지 Vol.29 No.3

본 논문에서는 액체의 표면장력을 이용하여 복수의 KGD 들을 웨이퍼 상태에서 일괄접합함으로써, 높은 수율의 삼차원적층칩을 빠른 생산성으로 제작할 수 있는, 고속 고정밀 접합기술인 자기조직화정합 (Selfassembly) 기술에 대해 소개를 하였다. 본 연구실에서 개발한 self-assembly 기술을 적용하여 5mm 각(角) 크기의 칩 500개를 1초 이내에 평균 $0.5{\mu}m$ 정도의 높은 정밀도로 8인치 웨이퍼상에 일괄접합시키는데 성공하였다. Self-assembly 기술에 의한 삼차원 칩 적층방식은, 기존의 pick-and-place 적층방식에서 높은 정밀도의 접합특성을 확보하는데 필요한 공정시간을 혁신적으로 단축하는 것이 가능하고, 웨이퍼 레벨에서 복수의 KGD 들을 일괄접합하는 것이 가능하므로, 향후 TSV 기술의 양산화를 실현하는데 적합한 고속 고정밀 접합 기술로서 기대가 크다. 현재 본 연구실에서는 두께가 $50{\mu}m$ 이하의 얇은 LSI 칩 및 메탈범프가 형성된 LSI 칩 등을 이용하여, self-assembly 기술에 의한 삼차원 적층형 집적회로 구현을 위한 접합기술을 개발 중에 있다.