2014년 경기지역에서 발생한 해외유입 홍역 바이러스의 분자역학적 특성 분석

윤희정,박포현,황선일,허정원,남수정,용금찬,윤미혜,Yun, Hee-Jeong,Park, Po-Hyun,Hwang, Sun-Il,Huh, Jeong-Weon,Nam, Soo-Jung,Yong, Kum-Chan,Yoon, Mi-Hye 한국미생물학회 2017 미생물학회지 Vol.53 No.1

Measles virus is a highly contagious, exanthematic virus, preventable by the use of an effective live-attenuated vaccine. However, measles virus remains endemic in many area of the world causing nearly 200,000 deaths per year and still a major cause of child mortality, mostly in developing countries. In March 2014, Republic of Korea was certified as a 'national measles elimination' by the WHO as a result of a high-quality case-based surveillance system and population immunity, which was achieved by a high vaccination rate (>95.0% since 1996). But, since the beginning of 2014, the Gyeonggi province has experienced a resurgence of measles cases. In this study, we investigated the characteristics of measles viruses isolated from confirmed measles in Gyeonggi province during January 1, 2014 ~ July 31, 2014, 60 isolates were obtained from 72 confirmed measles specimens. Genotypic distributions and genetic diversities of isolated measles virus were analyzed by sequencing of nucleoprotein (N) gene. 58 (96.7%) imported cases were identified. The predominant genotype was B3, which reflects the circulating measles virus in adjacent countries. The sequences of nucleoprotein (N) gene of isolated MeV were showed that the strains characterized showed the highest degree of identity (99%) with the Philippine related strains in 2013-2014. Therefore, infected traveler returning from the Philippines transmitted secondary infection in Korea. 홍역바이러스(Measles virus)는 전염성이 매우 높은 발진성 열병을 일으키는 바이러스이며, 약독화된 생백신 접종으로 충분히 예방할 수 있다. 그러나 대부분의 개발도상국에서 어린아이들의 주요 사망원인이며 해마다 20만명이 사망하는 풍토병이다. 우리나라는 1996년 이후부터 국가적으로 시행된 정기예방접종으로 인한 높은 백신 접종률(>95.0%)로 획득된 집단면역과 고도의 기술적 감시체계의 결과로 홍역이 퇴치되었으며, 2014년 3월 WHO로부터 '홍역퇴치국가'로 인증 받았다. 그러나 2014년 초부터 경기도에서 홍역환자가 다시 발생하였다. 본 연구에서는 2014년 1월부터 2014년 7월까지 경기도에서 발생한 홍역 확진 환자를 대상으로 홍역바이러스의 유행양상 및 유전형 분포양상을 분석하였다. 72건의 홍역바이러스 양성검체에서 60주의 홍역바이러스를 분리하였고, nucleoprotein(N) 유전자의 염기서열 분석을 통하여 유전형의 확인 및 유전자변이를 분석하였다. 총 60건의 홍역 확진검체를 분석한 결과 58건(96.7%)에서 B3 genotype을 확인하였다. 또한 nucleoprotein(N) 유전자 부위의 유전자 변이 분석결과, 경기도내 분리주들이 2013년에서 2014년 필리핀에서 유행한 홍역 분리주들과 99% 일치하였다. 따라서 필리핀 등에서 감염된 후 국내에 유입되어 2차 감염이 된 것으로 사료된다.

백작약 에탄올 추출물이 mouse embryonic fibroblast cells에 미치는 항산화 효과

윤희정 ( Hee Jung Yoon ),고은비 ( Eun Bi Go ),최민선 ( Min Sun Choi ),김동일 ( Dong Il Kim ),성정석 ( Jung Suk Sung ) 대한한방부인과학회 2012 大韓韓方婦人科學會誌 Vol.25 No.2

Objectives: Paeonia japonica has been widely used for gynecopathy and analgesic effects in Korean Traditional Medicine. The aim of the present study is to determine the antioxidant effect of Paeonia japonica extracts(PJE) by using mouse embryonic fibroblast cells(MEF cells). Methods: We evaluated Radical Scavenging Activity of PJE by the DPPH assay. Protective effect of the PJE on the hydrogen peroxide(H2O2) induced oxidative damage of MEF cells was analyzed by the MTT assay. The Morphological changes of MEF cells induced by P. japonica, H2O2 and P. japonica+H2O2 was evaluated by DAPI staining. And effect of PJE on the rate of apoptosis in MEF cells was measured using flow cytometry with Annexin V-FITC and PI double staining. Results: We observed that PJE contain significant DPPH radical scavenging activity. Cell viability of oxidative damaged cells treated with various concentrations of H2O2 was increased by treatment with PJE. Flow cytometric analysis of the cells treated with H2O2 in the absence or presence of PJE showed that the crumbled G1 peak was accumulated by the treatment with H2O2 alone, but restored by addition of PJE. Portion of cells that undergo apoptosis mediated by oxidative stress was decreased by treatment of PJE. The nuclear fragmentation occurred in the oxidative damaged MEF cells was also decreased by PJE treatment. Conclusions: Taken together, our results suggest that PJE exhibits significant antioxidant activity and functions to inhibit cell death mediated by oxidative damage induced apoptotic pathways.

정적 임무분리를 만족하는 사용자-역할 할당 방안

윤희정(Hee-Jung Yoon),전준철(Jun-Cheol Jeon),김용석(Yong-Seok Kim),전진우(Jin-woo Jeon),유기영(Kee-Young Yoo) 한국정보과학회 2004 한국정보과학회 학술발표논문집 Vol.31 No.2Ⅰ

오래전부터 많은 컴퓨팅 시스템에서 기분적으로 제공 되어지는 원리인 임무분리는 증대한 업무를 둘이 상의 사용자에게 나누어줌으로써 단독 사용자가 시스템을 손상시키는 것을 막도룩 하는 것이 목적이다, 역할기반 접근통제에서 임무분리 원리를 제공하는 종류로는 정적 임무분리, 동적 임무분리, 기능적 임무분리, 객체기반 임무분리 등이 있다. 여기서 우리는 정적 임무분리를 만족시키는 모델로서 상호 배제 역할 쌍을 이용한 모델과 역할유형을 이용한 모델 그리고 상호 무관 역할 쌍을 이용한 모델을 제안한다.

지카바이러스 감염

윤희정 ( Hee Jung Yoon ),염준섭 ( Joon-sup Yeom ) 대한내과학회 2016 대한내과학회지 Vol.91 No.1

Zika virus was first isolated in from nonhuman primate in 1947. It is in the genus Flavivirus, closely related to other flavivirus like Dengue, West Nile, Yellow fever and Japanese encephalitis virus. Since 2007 epidemic in Yap island, zika virus infections had spread to the countries in Micronesia and South Pacific. In 2015, Zika virus outbreak occurred in Brazil and now more than 40 countries in American continents reported autochthonous infection. The virus is transmitted mainly by Ae. aegypti mosquito with many other Aedes mosquito species known as vector. Recently, Zika virus infection is known to cause severe neurological complications and congenital malformation. In this paper, we will review current knowledge on Zika virus history, biology, clinical characteristics and preventive method. (Korean J Med 2016;91:5-11)

원발성 복막수염 3 예

윤희정(Hee Jung Yoon),지상원(Sang Won Chi),정준표(Jun Pyo Chung),박효진(Hyo Jin Park),김준우(Jun Uh Kim),송기섭(Kee Sup Song),이관식(Kwan Sik Lee),이상인(Sang In Lee),박미숙(Mi Suk Park),윤상욱(Sang Wook Yoon),유정식(Jeong Sik Yu),김기 대한소화기학회 2001 대한소화기학회지 Vol.38 No.2

Primary epiploic appendagitis, an acutely torsed and ischemic colonic epiploic appendages, is a benign condition that may present with peritoneal findings. Until recently, the accurate diagnosis could not be made preoperatively. However, since the advent of more refined imaging modalities, primary epiploic appendagitis may be distinguished preoperatively. This entity is a self limiting disease and thus, the awareness of this entity may help avoid an unnecessary laparotomy. Herein we present 3 cases of primary epiploic appendagitis. The first case of 40-year-old man has not been correctly diagnosed for 6 years even after a laparoscopic examination. The other 2 cases of 29-year-old and 40-year-old men, were diagnosed by CT examinations and managed conservatively. (Korean J Gastroenterol 2001;38:136-140)

대구지역 여자노인의 건강상태 및 영양소 섭취 실태조사

윤희정(Hee Jung Yoon),이희경(Hee Kyung Lee),이성국(Sung Kook Lee) 대한지역사회영양학회 2007 대한지역사회영양학회지 Vol.12 No.1

This study was designed to evaluate health and nutritional status of elderly females according to their family type. The participants were 157 elderly women (41 living alone, 45 living with a spouse, and 71 living with family). Among them, the subjects answered that their health rated as good condition, the elderly living with a spouse had significantly higher proportions than those living alone and living with family. Serum cholesterol and fasting blood glucose of the respondents living with a spouse were lower than those of the respondents living alone or with family. A dietary assessment with a 24-hour recall method represented that the elderly living alone had lower nutrient intakes, especially the intake of Ca. Most of women living alone or with family were consuming less than 75% of the Korean Recommended Dietary Allowances (RDA). For the females living with a spouse, the number of nutrients consumed below 75% of the Korean RDA were significantly lower than that for those living alone and the elderly living with family. As a result of the above analysis, the health and nutritional statement of old women with a spouse was better than that of the old living alone or with family. (Korean J Community Nutrition 12(1) : 50~57, 2007)

가정방문 영양교육에 의한 공복 혈당 이상 노인의 영양상태 및 혈당 변화

윤희정(Hee Jung Yoon),이성국(Sung Kook Lee) 대한지역사회영양학회 2006 대한지역사회영양학회지 Vol.11 No.3

This study was conducted to evaluate the effects of home-visiting nutrition education for the elderly with high fasting glucose level in an urban community. The study subjects were 40 elderly people, whose information on general characteristics, anthropometric measurement, biochemical indices, nutrition knowledge, nutrition attitude, dietary habit, food intake and nutrient intake were obtained at baseline. The education group received 6 weekly visits of home-visiting nutrition education from 15 March to 25 April 2004. In the baseline-survey, the education group and non-education group showed no differences in their general characteristics, health-related characteristics, anthropometric measurements, biochemical indices, nutrition knowledge, nutrition attitudes, dietary habits, and food and nutrient intake levels. The difference of mean change of nutrition knowledge, nutrition attitude and dietary habit after home-visiting nutrition education had been studied. The nutrition knowledge score increased by 1.4 in the education group; however, those in the non-education group which increased by 0.4, thus, the differences of mean change were statistically significant (p < 0.05). The nutrition attitude score increased by 1.2 in the education group; however, those in the non-education group decreased by 0.4, thus, the differences of mean change were statistically significant (p < 0.01). The dietary habit score increased by 1.7 in the education group; however, those in the non-education group decreased by 2.8, thus, the differences of mean change were statistically significant (p < 0.01). The difference of mean change of anthropometric indices and biochemical indices in education group and the non-education group was not significant. Looking over the zcereals and their products, vegetables, seaweeds, meats and their products, and fish than the non-education group. The MAR increased by 0.06 in the education group; however, that in the non-education group increased by 0.01, thus, the differences of mean change were statistically significant (p < 0.05). The differences of mean change of fasting blood glucose and biochemical indices after home-visiting nutrition education were studied. Fasting blood glucose decreased by 7.6 mg/dL in the education group; however, in the non-education group which increased by 0.4 mg/dL, the difference of mean change was not significant (p=0.051). The above findings suggest that home visiting nutrition education increases the nutrition knowledge and nutrition attitude, as well as, it is effective to change dietary habits. If the education period is extended, not fasting blood glucose improvement was insignificant, but fasting blood glucose improvement ability could be found by changing dietary habits. (Korean J Community Nutrition 11(3) : 346~360, 2006)

셀룰러 오토마타를 이용한 일회용 패스워드 인증

윤희정(Hee-Jung Yoon),전준철(Jun-Cheol Jeon),김기원(Kee-Won Kim),유기영(Kee-Young Yoo) 한국정보과학회 2005 한국정보과학회 학술발표논문집 Vol.32 No.1

사용자의 ID와 패스워드를 인증 기반으로 하고 있는 현재의 컴퓨터 시스템들은 스니퍼나 IP 스푸핑 등 을 통해 패스워드 누출에 대한 많은 위험성을 내포하고 있다. 이처럼 패스워드 도용을 이용한 불법 접속시도 등 각종 위협에서 전산망을 안전하게 운용하기 위해 안전한 사용자 인증 기술의 확보가 필요한데, 이러한 대책들 가운데 주목 받는 기술이 일회용 패스워드이다. 본 논문에서는 병렬성을 가짐으로써 연산속도가 빠른 셀룰러 오토마타(Cellular Automata, CA)를 이용해 일회용 패스워드를 생성한다. 그리고 사용자와 서버의 상호인증을 위한 인증인자를 생성하는데 CA를 적용하여 효율적인 인증 방식을 제안한다. 또한 제안된 방식이 여러 보안 요구사항에 안전함을 보인다.

고육근충 위암의 예후와 예후에 영향을 미치는 인자

윤희정 ( Hee Jung Yoon ),정준표 ( Jun Pyo Chung ),최승호 ( Seung Ho Choi ),김철식 ( Chul Sik Kim ),강병승 ( Byung Seung Kang ),이태희 ( Tae Hee Lee ),지상원 ( Sang Won Chi ),조준식 ( Jun Sik Cho ),문범수 ( Byoung Soo Moon ),이관식 대한소화기학회 2003 대한소화기학회지 Vol.41 No.2

Background/Aims: The purpose of this study was to evaluate the prognosis of gastric proper muscle (pm) cancer and its prognostic factors to identify a high risk group of patients with recurrence. Methods: Consecutive 154 patients (mean age, 57.6 years; M;

다양한 사구체 질환자들의 소변에서 TGF-β-inducible Gene-h3 (βig-h3)의 발현 양상

홍희주 ( Hee Joo Hong ),김성도 ( Sung Do Kim ),이병철 ( Byoung Cheol Lee ),윤희정 ( Hee Jung Yoon ),배은희 ( Eun Hee Bae ),조병수 ( Byoung Soo Cho ) 대한신장학회 2006 Kidney Research and Clinical Practice Vol.25 No.2