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해산 녹조료 참홑파래 , Monostroma nitidum 의 원형질체 분리와 분화
조용철(Yong Chul Cho),공용근(Yong Gun Gong),윤장택(Jang Taek Yoon),선상미(Sang Mi Sun),정규화(Gyu Hwa Chung) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.1
High yields of protoplasts were obtained following enzymatic digestion of the vegetative thalli of marine green alga Monostroma nitidum. The enzyme mixtures containing 4% Cellulase R-10+3% Macerozyme R-10+3% Abalone acetone powder produced 4.41×10^6 protoplasts per 300 ㎎ of fresh tissue. The highest yield of protoplasts was obtained by 270 minutes treatment of the thalli in enzyme solution. Freshly isolated protoplasts were spherical in shape and ranged between 13∼33 ㎛ in diameter. The high efficiency of differentiation were obtained by incubating freshly isolated protoplasts in 0.4 M mannitol f/2 medium for 7 days and then transferring to 0.2 M mannitol f/2 medium. Protoplasts began to form new cell walls three days after initial culture and began to germinate after 10 days, and then form a leafy thallus after further culture in f/2 medium. The addition of antibiotics in media inhibited the differentiation of protoplasts in culture.
어장 환경 조성을 위한 해삼 난의 확산 수치실험에 관한 연구
김진호 ( Jin-ho Kim ),신문섭 ( Moon-seup Shin ),강신중 ( Sin-jung Kang ),윤장택 ( Jang-taek Yoon ),유병로 ( Byong-ro Ryu ) 한국환경기술학회 2016 한국환경기술학회지 Vol.17 No.1
본 연구에서는 조석과 바람 잔차류의 계산결과를 이용하여 Euler-Lagrange 방법으로 해삼 난의 분산과정을 수치실험 하였다. 계산된 M<sub>2</sub> 분조의 위상과 진폭은 관측치와 유사하였다. 충남 해역의 M<sub>2</sub> 분조의 바람이 포함된 조석 잔차류는 모항과 만리포, 천리포, 신두리 해수욕장에서 시계방향으로 순환류형태가 나타났다. 가로림만의 조석 잔차류는 북쪽으로 흐르는 형태를 하고 있었다. 궁시도와 가의도 북쪽연안에서는 시계방향으로 순환류형태가 나타났다. 해삼 난의 확산특성은 바람과 M<sub>2</sub>의 분조의 잔차류 흐름 형태와 유사한 확산특성을 보여 주고 있다. 따라서 잔차류의 흐름형태가 난 확산특성에 영향을 크게 미친다고 판단된다. In this study, dispersion process of Apostichopus japonicus egg by Euler-Lagrange using a calculation result of the tidal residual currents and winds were numerical experiments. The calculated co-range and co-tidal charts of M<sub>2</sub> tide was similar to the observed ones. The residual flow pattern by M<sub>2</sub> tide and wind formed clockwise circulation in the coastal area of manripo, cheonripo, sinduri in chung nam coastal area. The tidal residual currents in the entrance of Garolim bay were flowing north. The tidal residual currents in coastal area of Gungsi and Gaui islands formed clockwise circulation. The diffusion characteristics of Apostichopus japonicus egg was similar to the residual currents pattern by M<sub>2</sub> tide and wind. Therefore, residual currents pattern is determined to significant influence on diffusion characteristics of Apostichopus japonicus egg.
강원도에 시설된(1992-1999년) 요철형어초와 사각형어초에 관한 보존상태
이지현(Ji-Hyun Lee),김완기(Wan-Ki Kim),김대권(Dai-Kweon Kim),조규태(Qtae Jo),윤장택(Jang-Taek Yoon),공용근(Yong-Gun Gong),전찬길(Chan-Kil Chun) 한국항해항만학회 2008 한국항해항만학회 학술대회논문집 Vol.2008 No.공동학술
Stability and durability of the artificial reefs (ARs) for wildstock enhancement have been a key issue. We surveyed the stability of artificial reefs installed at Gangeung sites along the Gangwon coast of the East Sea (Sea of Japan). The ARs surveyed were 2 types. The side scan sonar (SSS) and scuba diving surveys confirmed that the ARs stability was sediment and type-dependent. By type, dice reef was most stable, while unevenness reef was most vulnerable.
강원도에 시설된(1992-1999년) 요철형어초와 사각형어초에 관한 보존상태
이지현(Ji-Hyun Lee),김완기(Wan-Ki Kim),김대권(Dai-Kweon Kim),조규태(Qtae Jo),윤장택(Jang-Taek Yoon),공용근(Yong-Gun Gong),전찬길(Chan-Kil Chun) 한국마린엔지니어링학회 2008 한국마린엔지니어링학회 학술대회 논문집 Vol.2008 No.-
Stability and durability of the artificial reefs (ARs) for wildstock enhancement have been a key issue. We surveyed the stability of artificial reefs installed at Gangeung sites along the Gangwon coast of the East Sea (Sea of Japan). The ARs surveyed were 2 types. The side scan sonar (SSS) and scuba diving surveys confirmed that the ARs stability was sediment and type-dependent. By type, dice reef was most stable, while unevenness reef was most vulnerable.
강원도에 시설된(2000-2005년) 일반적인 4가지 종류의 인공어초에 관한 보존상태
김완기(Wan-Ki Kim),이지현(Ji-Hyun Lee),김대권(Dai-Kweon Kim),조규태(Qtae Jo),윤장택(Jang-Taek Yoon),공용근(Yong-Gun Gong),전찬길(Chan-Kil Chun) 한국마린엔지니어링학회 2008 한국마린엔지니어링학회 학술대회 논문집 Vol.2008 No.-
Stability and durability of the artificial reefs (ARs) for wildstock enhancement have been a key issue. We surveyed the stability of artificial reefs installed at Gangeung sites along the Cangwon coast of the East Sea (Sea of Japan). The ARs surveyed were 4 types. By type, Big bunk steel reef was most stable, while new unevenness reef was most vulnerable.
강원도에 시설된(2000-2005년) 일반적인 4가지 종류의 인공어초에 관한 보존상태
김완기(Wan-Ki Kim),이지현(Ji-Hyun Lee),김대권(Dai-Kweon Kim),조규태(Qtae Jo),윤장택(Jang-Taek Yoon),공용근(Yong-Gun Gong),전찬길(Chan-Kil Chun) 한국항해항만학회 2008 한국항해항만학회 학술대회논문집 Vol.2008 No.공동학술
Stability and durability of the artificial reefs (ARs) for wildstock enhancement have been a key issue. We surveyed the stability of artificial reefs installed at Gangeung sites along the Gangwon coast of the East Sea (Sea of Japan). The ARs surveyed were 4 types. By type, Big bunk steel reef was most stable, while new unevenness reef was most vulnerable.