활혈탕가미(活血湯加味)가 Collagen 유발 관절염의 발생과 치료에 미치는 영향

윤일지 ( Il Ji Yoon ),최승훈 ( Seung Hoon Choi ),오민석 ( Min Suck Oh ) 한방재활의학과학회 2004 한방재활의학과학회지 Vol.14 No.3

Objectives: This study was carried out to investigated the Anti-inflammation, Development and Curative Effects of HwalHyelTangkami (Huoxuetangjiawei) on the Collagen-Induced Arthritis in Wistar rats and ICR mouse. Methods: In experiment 1, Studies on the inhibitory effects of nitric oxide synthesis, pro-inflammatory cytokines, cyclooxygenase 2, leukocyte migration, vascular permeability, radical scavenging activity of HwalHyelTangkami(Huoxuetangjiawei) extract(or concentration). In experiment 2 and 3, rats were divided into non-treated normal group, porcine type 2 collagen immunized control group and collagen immunized and HwalHyelTangkami(Huoxuetangjiawei) treated group. Results: In experiment 1, the results were as follows; 1. Nitric oxide synthesis and Leukocyte migration has significantly inhibition effect by HwalHyelTangkami(Huoxuetangjiawei) extract. 2. Pro-inflammatory cytokines and cyclooxygenase 2(COX-2) were inhibited by HwalHyelTangkami(Huoxuetangjiawei) extract. In experiment 2, the results were as follows; 1. Body weight change in treated group has no significantly change. 2. Incidence of arthritis was 75 percent in control group and 25 percent in treated group. 3. Arthritis score of treated group was significantly decreased(p<0.05) compared with control group at 18days. 4. Paw edema volume of treated group was significantly decreased(p<0.05) compared with control group at 20days after immunization. 5. Thickness of ankle joint of treated group was significantly decreased(p<0.05) compared with control group at 20days after immunization. In experiment 3, the results were as follows; HwalHyelTangkami(Huoxuetangjiawei) treated group has no significantly change on body weight, paw edema volume, thickness of ankle joint and serum TNF-α level has no significantly change. Conclusions: These results indicated that HwalHyelTangkami(Huoxuetangjiawei) has the anti-inflammation effects on the ICR mouse. And the higher inhibitory effects on the onset of collagen-induced arthritis in rats and the lower inhibitory effects on the progression of collagen-induced arthritis in rats.

교통사고 후유증 112례에 대한 임상분석

윤일지 ( Yoon Il-Ji ),송태원,오민석 ( Oh Min-Seok ) 대한한의학회 한방재활의학과학회 1998 한방재활의학과학회지 Vol.8 No.1

대전대학교 부속 한방병원 한방재활의학과에 1996년 10월 1일부터 1998 년 5월 31일까지 교통사고로 인하여 입원 및 외래로 치료받았던 환자 11 2명을 대상으로 임상분석한 결과 다음과 같은 결론을 얻었다. 1. 성별 및 연령별 분포에서는 남녀의 비가 서로 비슷했으며, 30대에서 가장 많이 발생하였다. 2. 내원 당시 환자의 상황별 분포에서는 타병원에서 치료받고 본원에 내원한 환자가 89명(79.46%)으로 나타나 대부분 교통사고시 양방병원을 일차 이용하는 것으로 나타났다. 3. 내원 당시의 구분별 분포에 있어서는 외래로 치료받은 환자가 79명 (70.54%), 입원치료받은 환자가 32명(28.57%)으로 나타났다. 4. 차종별로 본 사고 환자 분포에 있어서는 승용차에 의한 사고 환자가 80명(71.43%)으로 가장 많이 나타났다. 5. 교통사고 후 본원에 내원하기까지 기간에 있어서는 5일 이내가 28명 (25.00%)으로 가장 많았고, 31-60명이 23명(20.54%)으로 다음으로 많았 으며, 이외의 기간은 비슷한 결과를 나타내었다. 6. 교통사고후 손상기전별 분포에 있어서는 충돌에 의한 경우가 94명(8 3.93%)으로 가장 많았고, 그 다음으로 급정차 및 급회전이 12명(10.71%) 으로 나타났다. 7. 교통사고시 충돌부위별 분포에 있어서는 두부 및 경항부가 82례(73. 21%)으로 가장 많이 나타났고, 요추부 44례(23.28%), 상하지부 22례(11. 64%) 등의 순으로 나타났다. 8. 환자의 주소증상별 분포에 있어서는 사지동통이 81례(20.40%)으로 가장 많았으며, 항강 63례(15.87%), 현훈과 두통 48례(12.09%), 야간통 47례(11.84%), 요통 40례(10.08%), 흉통 27례(6.80%)등의 순으로 나타났 다. 9. 초진시 호소증상별 분포에 있어서는 5가지 이상인 경우가 41명(36.6 1%)으로 가장 많이 나타났다. 10. 동통의 부위별 분포에 있어서는 하지, 상지, 경추, 요추가 각각 12 8례(29.84%), 110례(25.64%), 66례(15.38%), 55례(12.82%)순으로 나타났 으며, 특히 동통의 종류 중 비감, 무력감, 마목감 등의 증상이 상지와 하지에 많이 나타났다. 11. 교통사고로 유발된 질환별 분포에 있어서는 타박상이나 찰과상을 입은 환자가 76명(58.46%)로 가장 많았으며, HIVD 20명(15.38%), 정신신 체장애가 19명(14.62%)등으로 나타났다. 12. 치료기간별 분포에 있어서는 11-20일과 91일이상이 동일하게 20명 (17.86%)으로 가장 많았고, 1일 19명(16.96%), 2-10일 18명(16.07%)등의 순이다. 13. 치료방법별 분포에 있어서는 침, 한방물리치료, 한약이 각각 111명 (99.11%), 86명(76.79%), 79명(70.54%)순으로 나타났다. 14. 치료약물에 대한 처방별 분포에 있어서는 초기에는 활혈거어지제, 중기에는 이기순기지제, 후기에는 보기혈지제가 가장 많이 사용되었다. 15. 한방물리치료별 분포에 있어서는 통경락요법인 부항 82례(73.21%), 전기요법이 75례(66.96%), 온경락요법인 Hot-pack 70례(62.5%), 극초단 파가 55례(49.11%)순으로 각각 나타났다. 16. 종합효과 판정에 있어서는 현저한 효과가 36명(32.14%), 효과있음 이 34명(30.36%), 효과없음이 26명(23.21%), 완치가 8명(7.14%), 실패가 7명(6.25%)으로 각각 나타났다.

경항통 환자의 중성어혈약침에 대한 임상적 고찰

윤일지 ( Il Ji Yoon ) 대전대학교 한의학연구소 2007 한의학연구소 논문집 Vol.16 No.2

비만환자의 체성분 연구

윤일지 ( Il Ji Yoon ),오민석 ( Min Suk Oh ),송태원 ( Tae Won Song ) 한방비만학회 2001 한방비만학회지 Vol.1 No.1

We want to understand body composition and states of obese patients and the clinical studies of obesity attribute to Dept. of Oriental Rehabilitation Medicine. Methods: The clinical studies were done on 42 cases of obese patients who were treated by obesity clinic in Dept. of Oriental Rehabilitation Medicine Dae-Jeon Oriental medical hospital in Dae-Jeon University from May 2000 to September 2000. We checked body composition using Inbody3.0. Results: Sexual ratio on this report is 1:4.98(male: female). Abnormal eating is Major cause of obesity that has caused orthopedic disease. Increasing of BMI, fluid, protein and mineral mass decrease.

창출도인탕가미방(蒼朮桃仁湯加味方)이 류마토이드 관절염에 미치는 실험적 연구

박은숙 ( Eun Sook Park ),윤일지 ( Il Ji Yoon ) 한방재활의학과학회 2009 한방재활의학과학회지 Vol.19 No.4

Objectives: This study was carried out to understand the immune responses of the Changchuldointanggami-bang(after referred to CDIT) on antioxidants, THP-1 cell and rheumatoid arthritis in Collagen-induced Arthritis(CIA) mice. Methods: The antioxidative effect of CDIT on DPPH free radical and SOD-like activity was measured. CDIT was administered to THP-1 cell, cytokine and mRNA associated with inflammation were measured. CDIT was orallyadministered to mice with arthritis by collagen Ⅱ and then cytokine, PBMC in the serum were measured. Results: 1. The scavenging activity of CDIT on DPPH free radical was dose-dependent. 2. The effect of CDIT on SOD-like activity was dose-dependent. 3. The production of IL-1β, IL-6, TNF-α was decreased significantly in THP-1 cell. 4. The expression of IL-1β mRNA, IL-6mRNA, TNF-α mRNA were decreased significantly in THP-1 cell. 5. IL-1β, IL-6, TNF-α were decreased significantly in the serum of CIA mice. 6. CD3+, CD4+, CD8+ were increased significantly, butCD3+/CD69+, CD3+/CD49b+, B220+/CD23+ were decreased significantly in PBMC of CIA mice. Conclusions: Taking all these observations into account, CDIT is considered to be effective in rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

관절1호방이 Collagen Ⅱ로 유발된 관절염 억제에 관한 연구

한규진 ( Kyu Jin Han ),진광선 ( Kwang Seon Jin ),신혜란 ( Hye Ran Shin ),윤일지 ( Il Ji Yoon ),최승훈 ( Seung Hoon Choi ),오민석 ( Min Seok Oh ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.4

Objectives : The aim of this study was to know the immunity responses of Gwanjulbang-1(Quanjiefang-1)(hereinafter referred to GJB-1) to on Rheumatoid Arthritis in Collagen-induced Arthritis(CIA) Mice Methods : For this purpose, experiments were performed to measure the cytotoxicity against mLFC, hFLSs and the production of proinflammatory cytokines IL-1β, IL-6, TNF-α, IFN-γ in hFLSs and the production of pro-inflammatory cytokines IL-4, IL-10 and the value of CD3+(T), CD19+(B), CD3+/CD69+, CD4, γδ T cells, CD4+/CD25+ Results : 1. The cytotoxicity against mLFC, hFLSs were not measured. 2. The production of pro-inflammatory cytokines IFN-γ were reduced in hFLSs. 3. The production of pro-inflammatory cytokines IL-4, IL-10 were increased. 4. Statistically significant value of CD3+(T), CD19+(B), CD3+/CD69+, CD4, γδ T cells, CD4+/CD25+ compared with control group, wild type, MTX. Conclusions : Comparison of the results for this study showed that GJB-1 had immunomodulatory effects of suppressing or enhancing. So we expect that GJB-1 should be used as a effective drugs for not only rheumatoid arthritis but also another auto-immune disease. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

체성분 분석 변수와 요부 등속성 근력 측정을 근거로 한 비만과 요통의 관계

박지현 ( Ji Hyun Park ),안순선 ( Soon Sun An ),최용훈 ( Yong Hun Choi ),홍서영 ( Seo Young Hong ),허동석 ( Dong Seok Heo ),윤일지 ( Il Ji Yoon ) 한방재활의학과학회 2008 한방재활의학과학회지 Vol.18 No.4

Objectives : In order to investigate the relation of body composition analysis and isokinetic trunk muscle strength for the screening test of low back pain. Methods : This study was carried out with the data from comprehensive medical testing. 75 subject aged 20-59 performed the segmental bioelectrical impedance analysis, isokinetic trunk muscle strength test and questionnaire. Then we analyzed the relationship of data. Results : Low back pain(LBP) prevalence in high obesity index(Body mass index(BMI), Percentage of Body Fat(PBF), Waist Hip Ratio(WHR)) group was higher than LBP prevalence in normal obesity index group(p<0.001). In LBP group, Extension Peak Torque(Ext.PT), Extension Peak Torque per Body Weight(Ext.PT/BW) were significantly lower than Non-LBP group(p<0.001). And 90% of LBP group indicated abnormal Extension-Flexion Ratio(E/F ratio)(1.0 < Normal E/F ratio <1.6). When it comes to analyze relation between obesity index and muscle strength, Ext.PT/BW was significantly decreased accoring to PBF, WHR score. And correlation coefficient in Flex.PT, Flex.PT/BW, Ext.PT, Ext.PT/BW and PBF showed decresing function. Conclusions: Results from this investigation showed positive correlation between obesity and LBP prevalence. Decreased muscle strength and inbalanced E/F ratio were shown in LBP. Trunk muscle strength was changed according to body mass composition parameters. This results are expected to contribute to prevent and diagnose LBP by application the clinical index of body composition analysis.

Sweet BV 병행 치료 후 척수 손상 환자의 감각 분절 호전 1례

박인선,윤일지,Park, In-Sun,Yoon, Il-Ji 대한약침학회 2009 Journal of pharmacopuncture Vol.12 No.2

Objective : Patients with spinal cord injury are increasing in numbers. However, there is no reliable treatment guide in both conventional & complementary medicine. Also, there are not much clinical case of patients with spina cord injury in oriental medical field. We investigated effect of sweet BV on subacute stage patient with spinal cord injury. Method : 31-year old female patient with spinal cord injury was treated with herb medicine(TID), electro arcupunture (BID), sweet BV injection(QOD), Physical treatment(QD), and conventional-medicine. Result : We had a satisfactory result with using sweet BV injection. The patient's ASIA grade improved from 34 to 52. And Frankle classification of the patient shifted from A to B. Conclusion : We reach a conclusion Using Sweet BV improve the sensation of patient with spinal cord injury. And more study about this disease is needed.

교통사고 후유증 환자의 임상적 분석과 한방치료효과

송영일 ( Young Il Song ),진광선 ( Kwang Seon Jin ),신혜란 ( Hye Lan Shin ),윤일지 ( Il Ji Yoon ),최승훈 ( Seung Hoon Choi ),오민석 ( Min Suck Oh ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.2

Objectives : This study was designed to find out the characteristic of patients who had admitted due to traffic accident and to promote the superiority of oriental medical treatment on the sequelae of traffic accident. Methods : We analyzed 332 traffic accident patients statistically according to medical charts, who had been hospitalized from January 1, 2000 to October 30, 2004. Results and Conclusions : The analysis showed that there was no significant difference in sex(p>0.05), but the older group and the shorter group of interval time from onset to addmission had the better remedial result(p<0.05). The most common diagnosis that had the better remedial result was lumbar sprain, followed by multiple contusion, cervical sprain, herniated lumbar disc, herniated cervical disc(p<0.05), and the most effective oriental medical treatment was moxibusition, followed by blood cupping therapy, taping therapy, chuna therapy except acupuncture, herbal medication, oriental physical therapy(p<0.05). In using of TENS, ICT, U/S, complex therapy had better remedial result than single therapy(p<0.05). The longer period of treatment group had better remedial result generally(p<0.05).

비만환자의 체성분 연구

오민석,송태원,윤일지,Oh, Min-Suk,Song, Tae-Won,Yoon, Il-Ji 한방비만학회 2001 한방비만학회지 Vol.1 No.1

Objectives: We want to understand body composition and states of obese patients and the clinical studies of obesity attribute to Dept. of Oriental Rehabilitation Medicine. Methods : The clinical studies were done on 42 cases of obese patients who were treated by obesity clinic in Dept. of Oriental Rehabilitation Medicine Dae-Jeon Oriental medical hospital in Dae-Jeon University from May 2000 to September 2000. We checked body composition using Inbody3.0. Results : Sexual ratio on this report is 1:4.98(male: female). Abnormal eating is Major cause of obesity that has caused orthopedic disease. Increasing of BMI, fluid, protein and mineral mass decrease.