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RISS 인기검색어
- 검색키워드
- 저자 : 윤이란 ( I Ran Yun ) (검색결과 4 건)
- 무료
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Micro-HPLC를 이용한 조제분유 중 비타민 A,E 동시분석법 개발
윤이란 ( I Ran Yun ),최유정 ( You Jeong Choi ),이민권 ( Min Kwon Lee ),정명호 ( Byeong Hun Kim ),김병훈 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3
Semi-micro-HPLC using a column-switching technique was developed for simul-taneous determination of vitamin A and E contents in infant formula. Vitamin A and E were extracted by PDA-HPLC with reversed phase column using organic solvent and their contents in Certified Reference Material(CRM) and infant formula were deter-mined and compared with hydrolysis method and rapid extraction. Developed method has many advantages of simple and rapid sample preparation and simultaneous deter-mination of vitamin A and E by micro-HPLC using reversed phase column.
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갈근 및 녹차추출물의 아질산염 소거작용 및 간독성 보호효과
윤이란 ( I Ran Yun ),최유정 ( You Jeong Choi ),허정호 ( Jung Ho Heo ),최철웅 ( Chul Yung Choi ),성태종 ( Tae Jong Seoung ),김윤근 ( Yun Geun Kim ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3
This study was designed to evaluated the nitrite scavenging activity and protective effect of the Puerariae Radix and green tea extract on lead acetate and cadmium-induced liver damage in mice. The quantitative analytical method for major antioxidants, isoflavones, puerarin, catechine and caffeine in galgun (Puerariae Radix) and green tera extract were established by HPLC. Contents of isoflavones, such as daidzin, genistin, daidzein and genistein were 4.23g/100g, 0.13g/100g, 0.07g/100g, and 0.03g/100g, and puerarin contents was 8.99g/100g, respectively. The total catechins and caffeine contents of green tea extract were 49.24g/ 100g and 6.53g/100g. The nitrite scavenging ability of galgun extract (pH 1.2, 100mg/ml) was 98.07% and it was higher than those of other extracts. It was the highest at the pH 1.2 and more than 64% in 25~ 100mg green tea extract, and was dependents on pH and concentration of the samples. The hepatoprotective effects of an aqueous extract from the root of galgun and standard puerarin were evaluated against lead acetate and cadmium-induced liver damage in mice. Galgun extract and standard at a dose of 100mg/kg and 10mg/kg, 50mg/kg were administered orally once daily for successive 5 days and then a lead acetate and cadmium were orally at 3 hrs after the every day administration of galgun. The substantially elevated serum enzymatic activities of alanine and aspartate aminotransferase were due to lead acetate and cadmium treatment was dose dependently restored to the near normal level. In addition, galgun extract also significantly prevented the elevation of hepatic malon-dialdehyde formation in the liver of lead acetate and cadmium intoxicated mice in a dose-dependent manner. The results of this study clearly indicated that green tea and galgun extracts had nitrite scavenging ability and galgun extract had potent hepato-protective effects against lead acetate and cadmium-induced hepatic damage in mice and standard puerarin was also showed similar to the results of the galgun extracts.
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LC/MS/MS를 이용한 식용란 중 fluoroquinolone계 항균물질의 분석법에 관한 연구
최유정 ( You Jeong Choi ),윤이란 ( I Ran Yun ),남상윤 ( Sang Yun Nam ),박영호 ( Young Ho Park ),김병훈 ( Byeong Hun Kim ),손성기 ( Seong Gi Son ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
An atmospheric pressure chemical ionization(APcI) LC/MS/MS method was developed for the simultaneous analysis of fluoroquinolones(norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin) residues in eggs. The spiked and blank samples were extracted from whole eggs using 50mM phosphate buffer(pH7.4). The extract was cleaned up by passage though Oasis(R) MAX extraction cartridge for solid-phase extraction followed by elution with 4% formic acid in methanol. The extract of sample was separated on a Waters AtlantisTM dC18 reversed-phase column(4.6×150㎜, 5㎛) and analyzed by APcI positive mode mass spectrometry. The mobile phase consists of aqueous 0.2% nonafluoropentanoic acid(NFPA) and methanol. Multiple reaction monitoring(MRM) using the precursor to product ion combinations of m/z 320 → 302, 332 → 314, 360 → 342 and m/z 358 → 340 were used to quantify norfloxacin(NOR), ciprofloxacin(CIP), enrofloxacin(ENR) and danofloxacin(DAN), respectively. The limits of quantification(LOQ) were 7.8ppb for NOR, 8.5ppb for CIP, 8.9ppb for ENR, and 4.8ppb for DAN. Average recoveries of fortified sample at levels of 0.025 to 0.1ppm were estimated 71.29% for NOR, 75.27% for CIP, 85.51% for ENR and 81.22% for DAN. These results could be applied for the confirmation and quantification in eggs.
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돼지 분변 및 도체에서 분리한 대장균, 장구균의 항생제 내성율 조사
정귀옥 ( Kwi Ok Jeong ),허정호 ( Jung Ho Heo ),이종민 ( Jong Min Lee ),윤이란 ( I Ran Yun ),최유정 ( You Jeong Choi ),김종수 ( Jong Shu Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3
The present study was conducted to investigate isolation and antimicrobial resistance ratio of E. coli, E. faecium and E. faecalis from feces(150 samples) and carcasses (150 samples) on slaughtered pigs from 6 slaughterhouse of 13 cities in the Gyeongnam during the period from January 2009 to December 2009. Isolation ratio of E. coli from feces and carcasses were 98 (65.3%) and 110 (73.3%), respectively, and simultaneously, E. faecalis and E. faecium from feces and carcasses were isolated 21 (14%), 52(34.7%) and 18 (12%), 14 (9.3%), respectively. All E. coli isolated from feces and carcasses except cefepime (0%) and ceftiofur (0%) were exhibited 2.4~83.6% of resistance to teteracycline (83.6%), ampicillin (68.2%), streptomycin (60%), chloram-phenicol (53.8%) and cephalothin (2.4%). All E. faecalis isolated from feces and carcasses except penicillin(0%) and vancomycin (0%) were exhibited 2.7~80.8% of resistance to teteracycline (80.8%), quinupristin/dalfopristin (78%), erythromycin (56.1%), streptomycin (43.8%) and bacitracin (2.7%). All E. faecium isolated from feces and carcasses except gentamicin (0%), vancomycin (0%), florfenicol (0%), linezloid (0%) and bacitracin (0%) were exhibited 3.1~53.1% of resistance to rifampin (53.1%), erythromycin and tetracycline (25%), penicillin (15.6%), ciprofloxacin (9.3%), and streptomycin, chloramphenicol, and quinupristin/dalfopristin (3.1%). According to the heard size, resistance ratio of E. coli strains isolated from feces and carcasses in slaughtered pigs-breeding farms over 1,500 heard to tetracycline, ampicillin, streptomycin and chloramphenicol showed higher resistance ratio (1.0 ~16.8%) than those of farms-breeding under 1,500 heard. From the our results, we suggest that a few of antimicrobials were used in the Gyeongnam than the other cities.
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