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윤문섭,Yun, Mun-Seop 과학기술정책연구원 1991 과학기술정책 Vol.- No.15
연구개발의 성과는 투입 자원의 크기로만 결정되는 것이 아니라, 이를 어떻게 효율적으로 관리하느냐에 보다 크게 의존된다. 따라서 한 국가의 기술혁신능력은 연구 개발의 관리 능력에 의해 좌우되며 이의 요체는 연구기획·평가기능이라 할 수 있다. 최근 첨단 기술의 주도권을 둘러싸고 선진국들 간의 개발 경쟁이 치열해짐에 따라 각국은 국가적 차원에서 대규모의 전략적 연구개발의 대형화·복합화 추세가 가속화되고 있고, 이에 대응하여 연구 기획·평가에 있어서 새로운 체계적인 방법론 및 기법이 요구되고 있다. 본 토에서는 '80년대 이후 선진국을 중심으로 변화·발전하고 있는 연구평가의 새로운 접근 방법을 파악하고, 미국,일본,EC,영국의 연구 평가 추진 현황 및 사례를 분석함으로써 우리 나라의 연구평가 시스템의 구축에 관한 시사점을 찾고자 한다. (편집자 주)
손성한,정순일,윤문섭,김태산,박용환,김영미,Sohn, Seong-Han,Jeong, Soon-Il,Yoon, Mun-Sup,Kim, Tae-San,Park, Yong-Hwan,Kim, Young-Mi 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.4
우리나라의 유전자변형농산물 의무 표시제가 시행됨에 따라 수입 유전자변형농산물 중 유전자변형 콩의 혼입유무를 판별할 수 있는 검정기술 개발이 요구되고 있다. 근사미(glyphosate)제초제에 저항성을 나타내는 토양미생물인 Agrobacterium CP4 유래의 5-enolpyruvyl shikimate-3-phosphate synthase(EPSPS) 유전자의 도입여부를 PCR로 진단할 수 있는 특이프라이머를 제작하여 제초제저항성 콩(Roundup Ready Soybean, RRS)을 검정할 수 있는 PCR조건을 확립하였으며 콩의 내재유전자인 lectin유전자와 RRS특이 프라이머를 이용하여 duplex PCR에 의한 제초제저항성 콩의 검정법을 확립하였다. 또한 수입 콩 및 콩나물에 대하여 근사미 제초제 처리로 저항성 개체를 판별하는 생물검정법도 확립하여 저항성 개체의 잎에서 분리한 genomic DNA에 대하여 EPSPS특이 프라이머를 이용하여 분석한 결과 RRS특이적인 PCR밴드를 확인하였다. 또한 수입 콩의 백립중과 종실의 제색을 고려할 때 단일품종이 아닌 여러 품종이 혼합되어 있음을 확인하였다. Along with the worldwide rapid increase of the cultivation area and commercial production of genetically modified (GM) crops, the amount of GM grains imported to Korea has also been increasing. Roundup-Ready soybean (RRS) was introduced with 5-enolpyruvyl shikimate-3-photphate synthase (EPSPS) gene derived from Agrobacterium CP4 to confer the resistance to herbicide, glyphosate. In this study, we tried to develop PCR-based analytical method to detection the presence of RRS among non-GM soybeans. In order to detect RRS specifically, oligonucleotide primers were specifically designed based on the nucleotide sequence of EPSPS transgene. Qualitative PCR method was established and its specificity and accuracy were confirmed by analysing the nucleotide sequence of PCR DNA fragments. Bioassay was also conducted by spraying glyphosate at seedling stage. Survived individuals showed obvious resistance to Roundup Ready, however all of non-GM seedlings died in two weeks after spray. Conclusively, the highly selective detection systems for RRS were successfully established by both PCR using specific primers to EPSPS transgene and bioassay using the herbicide resistance of RRS. In addition to, the imported soybean showed to be mixed to several varieties regarding to 100-seed weight and hilum color.
반하 및 차나무의 기내배양시 발생하는 세균의 동정 및 항생제 감수성 검정
김행훈,조규택,윤문섭,윤주원,조은기,Kim, Haeng-Hoon,Cho, Gyu-Taek,Yoon, Mun-Sup,Yoon, Ju-Won,Cho, Eun-Gi 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2
Contamination of bacterial infection is one of serious problems in in vitro culture system of root crops. From the contaminated tubes over 140 of petiole cultures of Pinellia ternata, a medicinal plant, 4 genera 8species 48 strains of bacteria, including Aeromonas and Pseudomonas, were isolated and identified and another 8 strains were not fully identified. Most of them were motile Gram positive bacteria as in common in early stage of in vitro cultures. Six strains of bacteria, 5 of Gram negative, including Enterobacter, and 1 of Gram positive, were identified from the embryonic axes cultures of tea plant. From the susceptibility test to pre-screened 5 antibiotics, all of the bacteria except for 2 species of Pseudomonas were susceptible to cefotaxime 60∼100mg/L. While 60mg/L erythromycin only was effective to Pseudomonas. Combination of erythromycin 20mg/L and cefotaxime 60mg/L totally suppressed the growth of all bacterial strains tested. Susceptibility test of bacteria from tea embryonic axes cultures showed similar results. Combination of erythromycin 35mg/L and cefotaxime 60mg/L was effective to 15 bacterial strains and partially effective to 1 unidentified.
김창영 ( Chang Yung Kim ),이정란 ( Jeong Ran Lee ),윤문섭 ( Mun Sup Yoon ),조규택 ( Gyu Taek Cho ),백형진 ( Hyung Jin Baek ),고호철 ( Ho Cheol Ko ),조양희 ( Yang Hee Cho ),전영아 ( Young Ah Jeon ),김정곤 ( Chung Kon Kim ) 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.3
The application and use of agricultural genetic resources have been reviewed since Korea was freed from the rule of Japanese imperialism in 1945. The whole changes that took place in the Korean agriculture, such as, characteristics of agriculture, application trends of genetic resources, roles of agricultural genebank, and future utilization perspectives of plant genetic resources were reviewed and summarized. The whole changes that took place in the Korean agriculture since the liberation from the Japanese rule were as follows. Before 1960s, Korean agriculture was a subsistence farming system with the landraces, introduction of foreign cultivars, and the beginning stage of domestic breedings. In 1960-1970s, the importance of genetic resources came up because the Korean government had to solve the national shortage of foods by developing and disseminating high-yielding cultivars such as `tongil rice`. In 1980-1990s, four-season-long cultivation of horticultural products were reared and predominantly based on the public needs. Since 2000s, diverse varieties of genetic resources became more important because of the need to cultivate crops with healthy function and high nutritional value. Hence, the interest in the diversity of agricultural genetic resources increased. The Rural Development Administration (RDA) has designated 91 local sub-banks for managing the overall national agricultural genetic resources. RDA installed the seed bank that contain more than 500,000 accessions with high technologies to preserve plant germplasms. Currently, the RDA deposits approximately 160,000 accessions of seeds, and developed and disseminated 2,477 cultivars using these genetic resources. In summary, the major role of national agricultural genebank are as follows: 1) to manage national agricultural genetic resources. 2) to secure diverse genetic resources of current and future values, to conduct multiplication and characteristic assessment of genetic resources, and to utilize genetic resources through safe preservation and distribution service. 3) to conduct a variety of researches such as genetic diversity analyses, develop seed dormancy and preservation tools, assess seed characteristics and multiplication of seeds, develop technology to discover valuable resources using functional genes. 4) to raise the national status for preservation of agricultural genetic resources through international and domestic cooperation. Agricultural genetic resources will be used for the following purposes: to continuously produce competitive cultivars with high quality, to be utilized as raw materials to meet a constant food supply and demands of the general public`s well-being, to be applied to make agri-ecosystem healthy and prosperous, and to be used as a driving force for new growth of industries related to energy, new materials and stuffs.
김영미 ( Young Mi Kim ),손성한 ( Seong Han Sohn ),정순일 ( Soon Il Jeong ),윤문섭 ( Mun Sup Yoon ),김태산 ( Tae San Kim ),박용환 ( Yong Hwan Park ) 한국응용생명화학회 2002 Applied Biological Chemistry (Appl Biol Chem) Vol.45 No.4
Along with the worldwide rapid increase of the cultiation area and commercial production of genetically modified (GM) crops, the amount of Gm grains imported to Korea has also been increasing. Roundup-Ready soybean (RRS) was introduced with 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene derived from Agrobacterium CP4 to confer the resistance to herbicide, glyphosate. In this study, we tried to develop PCR-based analytical method to detection the presence of RRS among non-GM soybeans. In order to detect RRS specifically, oligonucleotide primers were specifically designed based on the nucleotide sequence of E{S{S transgene. Qualitative PCR method was established and its specificity and accuracy were confirmed by analysing the nucleotide sequence of PCR DNA fragments. Bioassay was also conductted by spraying glyphosate at seedling stage. Survived individuals showed obvious resistance to Roundup Ready, however all of non-GM seedlings died in two weeks after spray. Conclusively, the highly selective detection systems for RRS were successfully established by both PCR using specific primers to EPSPS transgene and bioassay using the herbicide resistance of RRS. In addition to, the imported soybean showed to be mixed to several varieties regarding to 100-seed weight and hilum color.