연곡천 송림보에서 어도의 개선에 따른 어류 종 조성 변화

윤영진,김지윤,김혜진,배대열,박구성,남창동,임경훈,이문용,이승용,문경도,이의행,안광국,Yun, Young-Jin,Kim, Ji Yoon,Kim, Hye-Jin,Bae, Dae-Yeol,Park, Gu Seong,Nam, Chang Dong,Lim, Kyung Hun,Lee, Moon-Yong,Lee, Seong-Yong,Moon, Kyeong-Do,Lee, 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.2

우리나라에서는 2020년에 생태계 건강성 측면에서 어류의 상하류 간 종적연결성 평가법에 대한 연구개발에 착수하였다. 본 연구는 최근 어류이동을 위한 아이스하버식 어도의 횡단구조물 개선이 이루어진 연곡천 송림보에서 2015~2020년 장기간 동안 보의 상하류 회유성 어종 이동성, 어류분포 특성, 섭식길드, 내성도 길드 및 종조성 변화를 분석하였다. 어류는 총 36종 5,136개체가 채집되었으며, 주요 회유성 어류는 황어(Tribolodon hakonensis), 은어(Plecoglossus altivelis altivelis), 연어(Oncorhynchus keta)로 3종이 확인되었다. 어도의 개선 이전(Pre-I)과 이후(Post-I) 비교분석에 따르면, 상류구간에서 일차담수어의 상대풍부도는 증가한 반면, 회유성 어종은 감소하였다. 송림보의 상/하류구간에서 어도(Fishway)를 이용하는 어류는 황어(58.4%), 은어(11.8%)로 나타났고, 상/하류 구간의 Wilcoxon Signed-Rank Test에 따르면, 회유성 어류는 통계학적으로 유의한 차이를 보여(p<0.05), 횡단구조물에 대한 생물학적 개선효과를 보였다. 한편, Mann-Kendall trend test 기반의 회유성 어류의 연별 변화는 유의한 수준(p>0.05)의 증가 혹은 감소의 경향은 확인되지 않았다. 따라서 어도의 개선 사업 시에는 물리적, 수리수문학적, 구조적 테스트뿐만 아니라 사전 및 사후의 어도이용 및 개선 효과에 대한 생물학적 시험도 반드시 필요할 것으로 사료된다. In 2020, South Korea initiated research and development of a longitudinal connectivity evaluation between upstream and downstream based on stream ecosystem health. This study analyzed the migration of upstream and downstream migratory fish species, fish distribution characteristics, trophic guilds, tolerance guilds, and species composition changes from 2015 to 2020 at Songrim weir in Yeongok stream, where the cross-structure of an ice harbor-type fishway for fish movement was recently improved. A total of 5,136 fish, including 36 species, were collected and three major migratory fishes were identified, namely, Tribolodon hakonensis, Plecoglossus altivelis altivelis, and Oncorhynchus keta. According to the comparative analysis before (Pre-I) and after (Post-I) improvement of the fishway, the relative abundance of primary freshwater fish increased in the upstream section, while the number of migratory fishes decreased. The fish species that used the fishway in the Songrim weir were Tribolodon hakonensis (58.4%) and Plecoglossus altivelis altivelis(11.8%). According to the Wilcoxon Signed-Rank Test migratory fish showed a statistically significant difference (p<0.05) in the upstream and downstream, showing a biological improvement effect of the crossstructure. On the other hand, the annual change of migratory fish based on the MannKendall trend test did not significantly increase or decrease (p>0.05). Therefore, in the fish passage improvement project, it is necessary not only for physical, hydrological, and structural tests, but also for pre- and post-biological tests on the use and improvement effect of fishway.

한국인의 Cytochrome P450 2C19 유전형 분석

윤영란,이준호,김문경,손지홍,이상섭,김종신,이순용,차인준,신재국,Yoon, Young-Ran,Lee, Jun-Ho,Kim, Moon-Kyung,Shon, Ji-Hong,Lee, Sang-Seop,Kim, Jong-Shin,Lee, Soon-Yong,Cha, In-June,Shin, Jae-Gook 대한임상약리학회 2002 臨床藥理學會誌 Vol.10 No.1

배경 : 간 cytochrome P450 2C19 (CYP2C19) 효소군은 유전적 다형성을 나타내는 대표적인 약물대사효소의 하나로, 본 연구에서는 다수의 한국인에서 CYP2C19 유전자 변이를 분석하고, 이들 변이 allele들의 출현빈도를 타 종족에서의 결과와 비교 분석하였다. 방법 : 282 명의 건강한 한국인 피험자에서 CYP2C19 (*2와 *3) allele 변이를 polymerase chain reaction restriction fragment length polymorphism (PCR­RFLP) 방법을 이용하여 분석하였다. 결 과 : 282명의 한국인 피험자 CYP2C19*1/*1 (41.8%), CYP2C19*1/*2 (35.5%), 및 CYP2C19*1/*3 (8.5%) allele 변이를 가지는 extensive metabolizer(EM)는 총 242명으로 나타났다. 40명 (14.2%)은 CYP2C19 의 poor metabolizer(PM)로 밝혀졌는데, 이들의 allele 빈도는 CYP2C19*2/*2, CYP2C19&2/*3, 및 CYP2C19*3/*3 가 각각 8.9%, 3.9%, 1.4% 로 나타 났다. 또한, 총 564개 allele에서 각 allele의 출현빈도는 CYP2C19*1, CYP2C19*2, 및 CYP2C19*3가 각각 63.8%, 28.6% 및 7.6%로 나타났다. 결 론 : 한국인의 CYP2C19 PM 유전형의 빈도는 중국인, 일본인 등의 동아시아인보다 다소 낮았으나, 이는 서양인에 비해서는 유의하게 높은 것으로 나타났다. Background : The cytochrome P450 2C19 (CYP2C19) is a well known microsomal oxidizing enzyme showing genetic polymorphism. We extensively evaluated the frequency distribution of the CYP2C19 allelic variants in a Korean population and compared to those of other ethnics. Methods : Genotyping of CYP2C19 (*2 and *3) was carried out in a total of 282 unrelated Korean subjects. CYP2C19 genotypes were determined using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Results : Two hundred forty two of 282 Korean subjects, carried extensive metabolizer(EM) genotypes including CYP2C19*1/*1 (41.8%), CYP2C19*1/*2 (35.5%), and CYP2C19*1/*3 (8.5%), respectively. Forty subjects (14.2%) were identified to have CYP2C19 poor metabolizer(PM) genotype, and the frequencies of CYP2C19*2/*2, CYP2C19&2/*3, and CYP2C19*3/*3 were 8.9%, 3.9%, 1.4% respectively. The allele frequencies of CYP2C19*1, CYP2C19*2, and CYP2C19*3 among 564 alleles evaluated were 63.8%, 28.6% and 7.6%, respectively. Conclusions : These results suggest that frequency of CYP2C19 PM genotypes is slightly lower than that of other East Asians including Chinese and Japanese, but significantly higher than Caucasian.

Evaluation of Stability Following Two-Jaw Surgery

이승용,김수관,김서윤,오지수,문경남,윤대웅,김훈,김정선,Lee, Sung-Yong,Kim, Su-Gwan,Kim, Seo-Yoon,Oh, Ji-Su,Moon, Kyung-Nam,Yoon, Dae-Woong,Kim, Hoon,Kim, Jeong-Sun Korean Association of Maxillofacial Plastic and Re 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.2

Purpose: Orthognathic surgery is required in patients with severe skeletal disharmony and facial asymmetry, which results in functional and esthetic improvement. Recently, bimaxillary surgery has become generalized. Establishment of the occlusal plane among several other factors included in the surgery plan is a major consideration for the diagnosis and treatment plan and it is also an important factor for postoperative stability. Methods: In this study, we assessed postoperative stability of occlusal plane, B-point, and pogonion point on 20 patients who underwent two-jaw surgery in the Chosun Dental Hospital from 2000 to 2007. Preoperative and postoperative states and at least a one year postoperative follow-up were compared. Results: The postsurgical relapse volume of the occlusal plane to the SN plane and the FH plane was $-0.26{\pm}2.8^{\circ}$ and $-0.44{\pm}3.29^{\circ}$, respectively and after two-jaw surgery, the stability of occlusal plane was maintained. The horizontal relapse degree was $0.85{\pm}0.46$ mm and $0.76{\pm}0.48$ mm, respectively, and the vertical relapse degree was $1.16{\pm}0.36$ mm and $1.13{\pm}0.71$ mm of the B point and the Pogonion point at the time after minimal 1 year. Conclusion: The vertical relapse amount was shown to be slightly larger than the horizontal relapse amount.

민들레 화분항원의 교차반응과 주요 알레르겐에 대한 IgE 결합 성분의 규명

김지혜 ( Ji Hye Kim ),윤문경 ( Moon Kyung Yoon ),김미애 ( Mi Ae Kim ),신유섭 ( Yoo Seob Shin ),예영민 ( Young Min Ye ),박해심 ( Hae Sim Park ) 대한천식알레르기학회 2015 Allergy Asthma & Respiratory Disease Vol.3 No.5

Purpose: The prevalence of pollinosis is increasing, and it is expected to increase further with climate change. Mugwort and ragweed pollens are well known as prevalent allergenic weed pollens in Korea. However, the clinical significance of dandelion pollen as an inhalant allergen has not yet been studied. The purpose of this study was to evaluate the clinical significance and cross-allergenicity between dandelion and major weed pollens. Methods: Ninety-seven patients with allergic rhinitis and asthma or with allergic rhinitis alone who were sensitized to dandelion pollens on skin prick tests (allergen/histamine ratio>3) were enrolled between December, 2012 and November, 2013. Serum specific IgE levels to dandelion pollen extracts were measured by using enzyme-linked immunosorbent assay (ELISA). ELISA inhibition tests were performed to evaluate cross allergenecity with other weed pollens. Results: When the positive cutoff value for serum specific IgE was set at the mean±3 standard deviation of absorbance values, 52 patients (53.6%) had a high serum specific IgE antibody level. ELISA inhibition tests showed significant inhibitions with serial addition of dandelion pollen extracts, and 5 different inhibition patterns were noted with addition of 4 weed pollen extracts: significant inhibitions with pollens of mugwort, ragweed, chenopodium and Hop J (25%, 13 of 52), inhibitions with pollens of mugwort, ragweed and chenopodium (17.3%, 9 of 52), inhibitions with 2 pollens of mugwort and ragweed (32.6%, 17 of 52), inhibitions with mugwort pollen (21.1%, 11 of 52), and inhibitions with dandelion pollen alone (4%, 2 of 52). Conclusion: These findings suggest that dandelion pollen may be a causative inhalant allergen to induce pollinosis in the autumn season. Cross-allergenicity with other weed pollens showed individual differences; most patients had cross-reactivity with mugwort, ragweed, and chenopodium pollens, while some with Hop J pollen. Few patients were sensitized to dandelion pollen alone. (Allergy Asthma Respir Dis 2015;3:358-364)

쑥갓 및 깻잎에 의한 구강 알레르기증후군 1예

홍가나 ( Ga Na Hong ),김미애 ( Mi Ae Kim ),윤문경 ( Moon Kyung Yoon ),이소희 ( So Hee Lee ),박해심 ( Hae Sim Park ) 대한천식알레르기학회 2014 Allergy Asthma & Respiratory Disease Vol.2 No.4

Oral allergy syndrome (OAS) is a subtype of food allergy composing of itching sense and edema in the oral cavity, lips, throat, pharynx, and larynx following ingestion of some fresh fruits or vegetables. Although the major pathogenic mechanism of OAS is known to be IgE-mediated response, here we experienced a case of OAS due to crown daisy (CD) and sesame leaf (SL) mediated by a non-IgE antibody mediated mechanism. A 33-year-old female visited our clinic to evaluate numbness of the tongue and gingiva after eating fresh CD and SL for 2 years. The patient had suffered from allergic rhinitis and atopic dermatitis for 20 years and took medications intermittently. There had been a history of food allergy to crab and shrimp. The serum total IgE level was elevated (404 kU/mL). The skin prick test showed strong positive reactions to tree and weed pollens, but not to CD and SL extracts. Enzyme-linked immunosorbant assay for detecting serum specific IgE to crude extracts of CD and SL showed negative results. The basophil activation test performed with crude extracts of CD or SL showed significant up-regulation of CD63-positive basophils by both CD and SL. In conclusion, we report a case of OAS due to CD and SL, not associated with pollen allergy, which is shown to be mediated by a non-IgE mediated mechanism.