가덕도 연안 해수에서 분리된 Vibrio vulnificus의 검출 특성 및 항생제 내성

오희경 ( Hee-kyung Oh ),정희진 ( Hee-jin Jeong ),정금재 ( Geum-jae Jeong ),신혜영 ( Hye-young Shin ),신중호 ( Joong Ho Shin ),정원교 ( Won-kyo Jung ),오정환 ( Junghwan Oh ),김영목 ( Young-mog Kim ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.6

We investigated the distribution, molecular characteristics, and antimicrobial resistance of Vibrio vulnificus isolated from seawater at Gadeok Island, the Republic of Korea between June to October. Interestingly no isolates were detected between December to February. The detection rate of V. vulnificus was high (80-100%) from July to September 2019 and from June to September 2020. This coincided with the relatively low salinity of the seawater, which ranged from 7.8-29.9 practical salinity units for that period. Additionally, V. vulnificus had a high detection rate at sampling stations near the Nakdong river. The detection rates of virulence genes, such as vvhA, viuB, and vcgC, among the isolates were 97.1%, 44.1%, and 57.4% in 2019 and 100%, 43.0%, and 50.0% in 2020, respectively. Notably, viuB and vcgC were detected in V. vulnificus isolated between June to October when water temperature was above 20°C. The antimicrobial susceptibility analysis of 80 isolates revealed that most of the strains were susceptible to most antimicrobial agents. However, some isolates showed intermediate resistance to cefepime (18.8%), cefoxitin (58.8%), and erythromycin (22.5%). Of note, 3.8% of the tested strains were resistant to cefoxitin. The minimum inhibitory concentration of highly cefoxitin-resistant strains was determined to be less than 32 μg/mL.

흰민들레의 부위별 건조방법에 따른 영양성분 및 항산화효과 비교

오희경 ( Hee Kyung Oh ) 대한영양사협회 2013 대한영양사협회 학술지 Vol.19 No.4

본 연구는 흰민들레의 지상부와 뿌리를 각각 자연건조 및 동결건조하여 건조방법에 따른 영양성분 및 항산화활성을 부위별로 비교함으로써 서로 다른 건조방법이 흰민들레의 부위별 영양성분 및 항산화 활성에 미치는 영향을 검토하고자 실시하였다. 1. 비타민 A, E 및 C의 함량은 지상부와 뿌리 모두 비타민 A 및 E의 함량에 비하여 비타민 C의 함량이 월등히 높게 나타났고, 자연건조 및 동결건조 흰민들레 모두 뿌리에 비하여 지상부에서 더 유의적으로 높게 나타났다. 뿌리의 비타민 C 함량은 건조방법에 따른 차이를 보이지 않았으나 지상부의 비타민 A 및 비타민 C 함량과 뿌리의 비타민 A의 함량은 자연건조에 비하여 동결건조에서 더 유의적으로 높았다. 2. 건조방법에 따른 흰민들레의 부위별 유기산을 분석한 결과 지상부와 뿌리에는 oxalic acid의 함량이 가장 높게 검출되었다. 동결건조 흰민들레의 citric acid 함량을 제외하고는 자연건조 및 동결건조 흰민들레 모두 뿌리에 비하여 지상부의 유기산 함량이 유의적으로 높게 나타났으며, 지상부의 citric acid의 함량과 뿌리의 tartaric acid의 함량을 제외하고는 자연건조에 비하여 동결건조에서 유의적으로 높은 함량을 보였다. 3. 총 polyphenol 및 총 flavonoid 함량은 자연건조 및 동결건조 흰민들레 모두 지상부가 뿌리에 비하여 더 유의적으로 높게 나타났다. 건조방법에 따른 총 polyphenol 및 총 flavonoid 함량은 흰민들레의 지상부와 뿌리 모두 자연건조에 비하여 동결건조에서 유의적으로 높게 나타났다. 4. DPPH radical 소거능과 Rancimat으로 측정한 항산 화지수는 자연건조 및 동결건조 흰민들레 모두 뿌리에 비하여 지상부에서 더 유의적으로 높았다. 지상부의 DPPH radical 소거능과 항산화지수는 자연건조에 비하여 동결건조에서 유의적으로 더 높게 나타났고 비타민 C보다는 유의적으로 낮았으나 BHA와 BHT보다는 유의적으로 높게 나타났다. 뿌리의 DPPH radical 소거능과 항산화지수는 건조방법에 따른 차이를 보이지 않았고 대조구인 BHA, BHT 및 비타민 C에 비해서는 낮았으나 시료를 미첨가한 음성대조군보다는 높게 나타났다. 이상의 결과 흰민들레에는 비타민, 유기산, polyphenol 및 flavonoid의 생리활성물질을 다량 함유하며 지상부에 높은 항산화활성을 나타내었다. 이런 우수한 생리활성을 나타내기 위해서는 자연건조보다는 동결건조가 효과적이라 판단되며 흰민들레를 이용하여 기능성 식품 및 의약품 소재로 활용할 수 있을 것으로 사료된다. This study compared the nutritional composition and antioxidative activity of different parts of Taraxacum coreanum (aerial parts and roots) according to different drying methods (natural drying and freeze-drying). There were no significant differences in vitamin C content in roots depending on the drying methods. However, vitamin A (P<0.01), E (P<0.001) and C (P<0.001) content of aerial parts, and vitamin A (P<0.001) and E (P<0.05) content of roots were significantly higher after freeze-drying compared to natural drying. For organic acids, the oxalic acid content of the aerial parts and roots were the highest. The total polyphenol and total flavonoid content in extracts from the aerial parts (P<0.01) and roots (P<0.05) were significantly higher after freeze-drying compared to natural drying. In addition, the total polyphenol and flavonoid content, DPPH radical scavenging activity, and antioxidative index from the natural drying and freeze-drying of Taraxacum coreanum extracts were significantly higher in the aerial parts compared to the roots (P<0.05). These results suggest that the nutritional composition and antioxidative activity of Taraxacum coreanum are higher in the aerial parts compared to the roots, and higher after freeze-drying compared to natural drying. Therefore, the aerial parts of Taraxacum coreanum could be suggested as an antioxidative functional food source.

Effect of Dietary Benzoic Acid on Beneficial Microflora and Immune Response in the Intestine of Weaning Pigs

Hee Kyung Oh(오희경),Young Hwan Choi(최영환),Ying Hai Jin(김영해),Yoo Yong Kim(김유용) 한국생명과학회 2012 생명과학회지 Vol.22 No.10

본 연구에서는 이유자돈 사료에 벤조산 첨가가 위장 내 소화물과 방광 내 뇨의 pH, 장내 유익 미생물 균총 수 및 장 조직 내 염증성 싸이토카인 유전자 발현양에 미치는 영향을 조사하였다. 자돈기 5주 동안 이유자돈 사료내 벤조산 0.3%과 0.5% 첨가는 자돈 위 내 pH는 감소시키지는 않았으나, 방광 내 뇨의 pH 수치는 유의적으로 감소하는 것으로 나타났다. Lactobacillus casei 수는 맹장부위에서 벤조산 0.5% 첨가구와 대조구에서 유의적으로 가장 높게 나타났다. Lactobacillus plantarum 수는 맹장부위에서 벤조산 0.5% 첨가구에서 가장 높게 나타났다. 또한, 직장부위에서 L.plantarum 수는 대조구를 제외하고 모든 처리구에서 유의적으로 높게 나타났다. Bacillus subtillis 수는 맹장부위에서 벤조산0.5% 첨가구와 대조구에서 유의적으로 가장 높게 나타났으며, 직장부위에서는 벤조산0.3%와 0.5% 첨가구에서 가장 높게 나타났다. IL-1β mRNA 유전자 발현 수준은 소장 상단에서는 벤조산 0.5%와 항생제 처리구에서, 소장중부에서는 벤조산 0.3%와 0.5% 처리구에서, 말단부위에서는 벤조산 0.3% 처리 구에서 대조구보다 유의적으로 낮게 나타났다. IL-6 mRNA 유전자 발현 수준은 소장중부에서는 벤조산 0.5% 처리구에서 대조구보다 유의적으로 낮게 나타났다. TNF-α mRNA 유전자 발현 수준은 소장 중단에서 벤조산 0.5%와 항생제 처리구에서 대조구 보다 낮게 나타났다. 본 실험을 통하여, 자돈 사료 내 benzoic acid 0.5% 첨가는 뇨의 pH 감소와 장내 유익 미생물 균총 수 조절 및 소장 내 면역체계 반응에 긍정적인 영향을 미치므로 자돈 건강을 향상 할 수 있으리라 사료된다. We evaluated the effect of dietary supplements with benzoic acid on intestinal beneficial bacteria concentration and immune response of weaning pigs. Supplementation with benzoic acid at 0.5% or control diet for 35 days resulted in a higher Lactobacillus casei concentration in the cecum. Supplementation with benzoic acid at 0.5% increased concentration of L. plantarum in the cecum. Pigs with the control diet and 0.5% benzoic acid had significantly increased concentration of B. subtillis in the cecum compared to the antibiotic group, while the concentration of B. subtillis in the rectum increased in pigs given 0.3 and 0.5% benzoic acid (p<0.05). Compared with the control group, the level of interleukin-1β mRNA showed a significant decrease in the proximal small intestine in pigs fed diets supplemented with benzoic acid at 0.5% or antibiotic. Feeding 0.5% benzoic acid resulted in a marked reduction in the expression of IL-6 mRNA in the middle small intestine (p<0.05). Supplementation with benzoic acid at 0.5% or antibiotic resulted in a lower level of tumor necrosis factor-mRNA in the middle intestine. Up to 0.5% benzoic acid may be included in weaning diets for improvement of intestinal beneficial bacteria, thus modulating genes of pro-inflammatory cytokines in the gastrointestinal tract.

백출 용매추출 방법에 따른 항산화 활성 및 항염증 효과

오희경(Hee-Kyung Oh) 한국응용과학기술학회 (구.한국유화학회) 2021 한국응용과학기술학회지 Vol.38 No.6

백출(Atractylodes japonica)은 국화과(Compositae)에 속하는 다년생 식물로 한국에서 이뇨 작용, 수렴작용, 항알러지, 신경보호, 항암, 면역증강 및 위장관 보호효과 등으로 민간요법에서 널리 사용되고 있다. 백출의 열수 및 70% 에탄올 추출물에 대한 DPPH 및 ABTS 자유라디칼 소거능으로 항산화 활성을 측정하였다. 게다가 NO 생성, iNOS와 COX-2 단백질 발현을 웨스턴 블롯으로 측정하였으 며, 염증성 사이토카인의 발현을 RT-PCR 방법으로 측정함으로 항염증 효과를 측정하였다. 열수 및 70% 에탄올 추출물들의 항산화 활성은 농도-의존적인 효과를 보였지만, 추출용매에 따른 차이는 나타 나지 않았다. 70% 에탄올에 의한 백출 추출물에서 매우 강한 NO 활성 억제효과를 보였다. 백출 열수및 에탄올 추출물은 NO 생성에 관여하는 iNOS 및 COX-2 단백질의 발현을 현저히 감소시켰다. 열수및 70% 에탄올 추출물 모두 염증성 사이토카인인 IL-6 및 IL-1b는 현저히 억제시키지만, TNF-α의 발현은 약하게 억제시키는 결과를 얻었다. 본 연구를 통하여 백출 70% 에탄올 추출물이 NO 생성을 현저히 억제하며, 염증성 사이토카인의 발현을 현저히 감소시키는 효과를 보이므로 염증성 질환 치료 및개선에 널리 사용될 수 있으리라 생각한다. Atractylodes japonica has been widely used in a traditional Korean herbal medicine exerting various pharmacological activities such as diauretic action, asriction, anti-allergy, neuroprotective activity, anti-cancer, immunomodulation and gastrointestinal protective effect. This study was to investigate the antioxidant, nitric oxide and inflammatory cytokines production of A. japonica extract by water and 70% ethanol. DPPH and ABTS free radical scavenging activity were increased in a dose-dependent manners with both extracts and there was no difference with extract solvents. 70% ethanol extract of A. japonica showed a very strong inhibitory effect on NO production. Both extracts of A. japonica significantly reduce the expression of iNOS and COX-2 proteins involved in NO prodction. A. japonica extract by water and 70% ethanol inhibited LPS-induced proinflammatory cytokines such as IL-6 and IL-1b. In this study, 70% ethanol extract of A. japonica significantly suppresses LPS-induced NO and inflammatory cytokine production. Therefore it can be widely used to treat and improve inflammatory diseases.

정향 열수 및 에탄올 추출물의 항산화 및 항염 효과

오희경 ( Hee-kyung Oh ) 한국식생활문화학회 2016 韓國食生活文化學會誌 Vol.31 No.5

The objective of this study was to investigate the antioxidant and anti-inflammatory activities of Eugenia caryophyllata Thunb. water and 70% ethanol extracts. The content of total polyphenol was significantly higher in water extract than in 70% ethanol extract. The DPPH radical scavenging activity of water extract was similar to that of Vit. C at a concentration of 1,000 μg/mL. The ABTS radical scavenging activities of water and 70% ethanol extract were similar to that of Vit. C at a concentration of 1,000 μg/mL. SOD-like activity of water extract was higher than that of 70% ethanol extract at a concentration of 1,000 μg/mL but lower than that of Vit. C. The DPPH radical scavenging activity, ABTS radical scavenging activity, and SOD-like activity increased as concentrations of water and 70% ethanol extracts increased. Cell cytotoxicity was not observed at all concentrations except at 100 μg/mL concentration of water extract. Inhibitory activity on NO production effect of water extract was significantly higher than that of 70% ethanol extract. These results show that E. caryophyllata Thunb. has potent biological activities, and their activities were different depending on extraction solvent.

스포츠 무릎보호대의 착용 실태 및 구매 행동에 관한 연구

오희경 ( Hee Kyung Oh ),오경화 ( Kyung Wha Oh ) 한국의류학회 2021 한국의류학회지 Vol.45 No.2

This survey provides research into developing knee braces to protect the knee against damage, reduce knee pain, and strengthen the knee. A total of 235 surveys were conducted from December 2019 to January 2020. The subjects were adults in their 20s and over who lived in the Seoul metropolitan area and had experience of using knee braces. In terms of age differences, subjects over 60 years of age purchased knee braces mostly to secure a reduction of pain (ROP), whereas subjects in their 20s and 30s purchased knee braces for physical shock protection (PSP). Subjects in their 40s and 50s purchased knee braces for improvement of muscle movement (IMM). There was no significant difference in shape, material, and locking method based on the reason for wearing the knee brace. These results show that it is conceivable and maybe preferable to produce different knee braces―with a variety of shapes, materials, and locking materials―according to the reason behind wearing a knee brace. Knee braces are worn currently without any differentiation despite the findings of this study. In particular, we argue the need for functional and differentiated development of knee braces specifically for PSP, ROP, and IMM.

연구논문 : 서양민들레의 항산화능이 저장 기간 동안 유과의 품질특성에 미치는 효과

김경미 ( Kyung Mi Kim ),장영윤 ( Young Eun Chang ),이재준 ( Jae Joon Lee ),오희경 ( Hee Kyung Oh ) 한국식생활문화학회 2014 韓國食生活文化學會誌 Vol.29 No.3

This study investigated the antioxidant effect of Taraxacumofficinale on the quality characteristics of Yugwa during storage. Total polyphenol content was higher than total flavonoid content. DPPH radical scavenging activity and antioxidative index of Taraxacum officinale ethanol extracts were significantly (p<0.05) lower compared with those of Vit.C, whereas they were similar with those of BHA. The L, a, and b levels of Yugwa significantly (p<0.05) decreased with increasing amount of Taraxacum officinale powder; 6% Taraxacum officinale powder showed the lowest L and b levels. Hardness was unaffected by Taraxacum officinale content during the storage period. Brittleness of Yugwa was significantly (p<0.05) lower in control, 1.5%, and 6% Taraxacum officinale powder, whereas 3% Taraxacum officinale powder showed no significant differences during storage. Peroxide value significantly decreased in 3% and 6% Taraxacum officinale powders compared to those of control and 1.5% Taraxacum officinale powder after 4 weeks of storage. Taste, flavor, and crispness showed significant (p<0.05) differences according to Taraxacum officinale concentration. In conclusion, addition of 3~6% Taraxacum officinale powder provides beneficial effects in terms of storage life of Yugwa without any detrimental effects on quality characteristics.

죽엽(솜대)의 항산화 성분 및 구강세균에 대한 항균 효과

박경란(Kyung Lan Park),강성태(Sung Tae Kang),김민주(Min Ju Kim),오희경(Hee Kyung Oh) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.9

본 연구에서는 죽엽(솜대)의 항산화 성분을 조사하고 구강질환 세균에 대한 죽엽 추출물의 항균 활성을 검토하였다. 죽엽의 일반성분은 탄수화물 76.26%, 조회분 10.61%, 조단백질 5.10%, 수분 6.30%, 조지방 1.73%였고, 비타민 A 및 E의 함량에 비하여 비타민 C 함량은 월등히 높게 나타났다. 유기산 중 citric acid 함량이 가장 많이 함유되어 있었고 다음으로 succinic acid, malic acid, acetic acid, formic acid 순으로 검출되었다. 총 폴리페놀 및 플라보노이드 함량은 각각 21.66 mg/g, 42.78 mg/g으로 나타났다. Streptococcus mutans에 대해 죽엽 추출물 0.04% 이상에서, Streptococcus sobrinus에 대해서는 0.16% 이상에서, Porphyromonas gingivalis와 Prevotella intermedia에 대해서는 0.02% 이상에서 강한 항균 활성이 인정되었다. S.mutans, S. sobrinus, P. gingivalis, P. intermedia는 죽엽추출물 0.32% 농도에서 각각 60시간까지 억제됨을 관찰할 수 있었다. 따라서 본 연구 결과는 죽엽 에탄올 추출물이 구강질환을 유발하는 세균에 대하여 우수한 항균작용을 나타내고 있으며, 따라서 죽엽이 구강질환 예방에 긍정적인 효과가 있을 것으로 생각한다. The objective of this study was to investigate the antioxidative components and anti-oralmicrobial effect of bamboo (Phyllostachys nigra var. henonis Stapf) leaves. The moisture, crude protein, crude fat, crude ash, and carbohydrate contents were 6.30%, 5.10%, 1.73%, 10.61%, and 76.26%, respectively. Vitamin C content was higher than Vitamin A and E contents. Among organic acids, citric acid content was the most abundant organic acid, followed by succinic acid, acetic acid, malic acid, and formic acid. Total polyphenol and flavonoid contents were 21.66 mg/g and 42.78 mg/g, respectively. Minimum inhibitory concentrations (MICs) of extracts of bamboo leaves for Streptococcus mutans and Streptococcus sobrinus were determined to be 0.04% and 0.16%, respectively. MICs of extracts of bamboo leaves for Porphyromonas gingivalis and Prevotella intermedia were determined to be 0.02%. Extract of bamboo leaves had strong antimicrobial activity against S. mutans, S. sobrinus, P. gingivalis, and P. intermedia at a concentration of 0.32%. At this concentration, extract of bamboo leaves inhibited growth of these pathogenic bacteria up to 60 h. The results of the present study demonstrate the antimicrobial effects of bamboo leaves ethanol extract against oral pathogenic bacteria, suggesting that bamboo leaves could be an effective natural agent for oral hygiene.

치자의 항산화 활성성분에 관한 연구

한용남,오희경,황금희,이미순,Han, Yong-Nam,Oh, Hee-Kyung,Hwang, Keum-Hee,Lee, Mie-Soon 한국생약학회 1994 생약학회지 Vol.25 No.3

An antioxidant activity of Gardenia Fruit (Gardenia jasminoides Ellis) which has been used for food coloring was studied. The antioxidant activity was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). Both water and methanol extracts of Gardenia Fruit showed the antioxidant activity. On solvent fractionation, the antioxidant activity was removed into the ethyl acetate and butanol soluble fractions. And the final water soluble fraction also showed the antioxidant activity in the low concentration, but it promoted the lipid peroxidation in the high concentration. Two compounds (I and II) having the antioxidant activity were isolated from the butanol fraction, and compound I also occurred in the ethyl acetate fraction. The antioxidant activity of compound II was more potent than that of I. By analyzing data for UV, IR and $^1H-NMR$, compounds I and II were identified as geniposide and crocin, respectively.

역세수량과 역세지속시간 변동을 통한 정밀여과막 역세공정 최적화

엄정열 ( Jung Yeol Eom ),오희경 ( Hee Kyung Oh ),강성훈 ( Sung Hun Kang ),이의신 ( Eui Sin Lee ),이범구 ( Bum Goo Lee ),강선홍 ( Seon Hong Kang ) 한국물환경학회(구 한국수질보전학회) 2011 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2011 No.-