Protective Effects of Portulaca oleracea L. Extract against Matrix Metalloproteinase Production and Reactive Oxygen Species Generation Induced by Ultraviolet B Radiation in Human Keratinocytes

Jung Hwan Oh(오정환),Fatih Karadeniz(파티 카라데니즈),Jung Im Lee(이정임),So Young Park(박소영),Youngwan Seo(서영완),Chang-Suk Kong(공창숙) 한국생명과학회 2018 생명과학회지 Vol.28 No.8

쇠비름(Portulaca oleracea.L)은 쇠비름과에 속하는 한해살이풀로서 리놀렌산과 같은 불포화지방산, 페놀성 화합물, 플라보노이드, 비타민 C, 미네랄 함량이 높은 것으로 보고되어 있다. 본 연구에서는 쇠비름 추출물을 이용하여 UVB를 조사한 인간각질형성세포에서 광노화 억제능을 확인하였다. Matrix metalloproteinases는 세포의 기질을 분해하는 효소로 MMP-1는 collagenase, MMP-2와 MMP-9는 gelatinases로 피부 진피층을 구성하는 type Ⅰ collagen을 분해시키는데 영향을 미친다. UVB를 조사한 인간각질형성세포에서 쇠비름 추출물을 처리했을 때 MMP-1, -2, -9의 발현이 감소하였으며, type Ⅰ procollagen의 발현은 증가하는 것으로 나타났다. 또한 쇠비름 추출물을 처리한 군에서 UV에 의한 ROS 생성이 감소하였는데 이는 Nrf-2의 활성화를 통한 항산화 인자 SOD-1과 OH-1의 발현 증가로 인해 세포내 ROS 생성이 감소한 것으로 사료된다. 따라서 본 연구 결과를 통해 쇠비름 추출물이 UVB를 조사한 인간각질형성세포에서 MMP 인자 및 항산화 인자의 발현 조절을 통해 광노화로부터의 세포 보호능을 가지는 것을 확인하였으며 나아가 화장품 소재로서의 개발 가능성을 확인하였다. Portulaca oleracea L. is an edible plant widely consumed in daily diet throughout Europe, Asia and America. In this study, protective effects of P. oleracea L. extracts against oxidative stress and matrix metalloproteinase (MMP) activity induced by ultraviolet B (UVB) radiation were investigated using HaCaT immortal human keratinocytes. In this context, the mRNA and protein productions of MMPs (MMP-1, -2, and -9) and type I procollagen, which are major markers of photoaging induced by UVB radiation in HaCaT keratinocytes, were evaluated. Furthermore, UVB-induced reactive oxygen species (ROS) generation and mRNA and protein expression levels of superoxide dismutase-1 (SOD-1), oxygenase-1 (OH-1), and nuclear factor-erythroid 2-related factor-2 (Nrf-2), all of which are associated with the antioxidant balance, were investigated. As shown by the results, UVB radiation induced ROS formation and led to increased production of MMPs and decreased collagen production in human keratinocytes, which resulted in skin photoaging or photodamage. The treatment with P. oleracea L. extracts downregulated MMP (MMP-1, -2, and -9) production and upregulated type I procollagen expression in UVB-induced HaCaT cells. Furthermore, treatment with the extracts decreased UVB-induced ROS generation and increased the expression of antioxidant enzymes, such as SOD-1 and OH-1, through the Nrf-2 pathway. Taken together, these results suggest that P. oleracea L. extracts could be a potential cosmeceutical agent for the prevention of skin photoaging or photodamage.

공동주택 하자관리 업무프로세스 개선

오정환,송영웅,최윤기,임형철,Oh, Jung-Hwan,Song, Young-Woong,Choi, Yoon-Ki,Lim, Hyoung-Chul 한국건설관리학회 2009 한국건설관리학회 논문집 Vol.10 No.5

최근 주택보급률의 상승과 공동주택 물량의 증가로 인하여 공동주택에 대한 건설기업의 공사 비중이 높아지고 있으며, 공급자 위주에서 수요자 중심으로 분양시장이 변화함에 따라 고객 가치 향상을 위한 고객접점의 하자관리의 중요성이 부각되고 있다. 그러나 국내 공동주택태 하자관리는 하자관리 업무 프로세스 관리 미흡,하자관리 조직의 관리 부족, 하자 정보 피드백 부족, 시공단계의 하자관리를 위한 대비부족으보 동일 하자 요인이 반복적으로 발생하며 고객이 요구하는 품질 및 만족도에 부합하지 못하는 실정이다. 따라서 본 연구의 목적은 이러한 하자관리의 문제점을 해결하고자 하자관리 업무 프로세스 개선 모델을 제시한다. 본 연구에서 제시된 하자관리 업무 프로세스 개선 모델은 하자관리와 품질관리의 통합적 관리를 통하여 정보의 피드백을 실현시키며 하자관리와 품질관리의 업무프로세스를 정렬한다. 또한 부적합 처리 업무를 개선함으로써 하자 관리 관련 업무의 질적 향상을 도모하고 궁극적으로 품질 향상을 통한 건설업체의 고객 만족도 및 이미지 제고에도 크게 기여할수 있을 것으로 기대된다. Recently construction companies expand position for apartment house in construction market because of house supply rate increase and its amount. In addition, because the focus of house sale market has moved to customer from supplier, the importance of defect management is being issued currently. However, current apartment defect management is not satisfied with customer's demand for the lack of business process management, management organization, information feedback, and readiness for defect in construction phase. To solve this problem, this study proposed business process management improvement model for defect management. Proposed improvement model make information feedback, defect management business quality improvement, and improper process improvement through integrating defect management and quality management. It is expected to contribute to customer's satisfaction improvement and reliance improvement for construction companies.

골격성 3급 부정 교합을 지닌 법랑질 형성 부전증 환자의 복합적 치료

오정환,김학렬,황윤태,김여갑,류동목,이백수,윤병욱,전준혁,Oh, Jung-Hwan,Kim, Hak-Ryeol,Hwang, Yoon-Tae,Kim, Yeo-Gab,Ryu, Dong-Mok,Lee, Baek-Soo,Yoon, Byung-Wook,Jeon, Joon-Hyeok 대한악안면성형재건외과학회 2007 Maxillofacial Plastic Reconstructive Surgery Vol.29 No.1

법랑질 형성부전증은 전치부 개교합과 같은 골격적인 문제를 자주 동반하며, 이러한 경우 구강악안면외과, 보철과, 보존과, 교정과 의사들이 함께 치료하여야 한다. 본 증례는 법랑질의 약화와 치아 우식증 등의 이유로 일반적인 교정치료를 시행할 수 없어 보철적 방법으로 치료하였다. 보존적, 보철적 방법을 이용하여 술전 교정과 같은 안정된 교합을 형성하였다. 악교정 수술을 시행하고 SAS 등을 이용하여 악간고정을 시행하여 양호한 결과를 얻을 수 있었다. Amelogenesis imperfecta (AI) is a hereditary disease that affects enamel formation. The patients with AI have esthetic and functional problems due to damage of multiple teeth. So most AI patients resolve these problem through the conservative and prosthodontic treatments. In our case, It was difficult to obtain good results in means of conservative and prosthodontic treatments, because the AI patient had skeletal Class III malocclusion. Moreover, because of vertical dimension loss due to severe dental caries and maxillofacial skeletal disharmony, the ordinary prosthodontic treatment was troublesome. So we planned orthognathic surgery to resolve these problems. After the endodontic treatment, temporary restoration was delivered for stable post-operative occlusion. Then orthognathic surgery was done, and final restoration was delivered in stable period. We obtained satisfactory results in esthetic and functional aspects through multidisciplinary management(conservative treatment, prosthodontics and orthognathic surgery).

후생유전학 (Epigenetics)과 DNA methylation의 이해

오정환,권용대,윤병욱,최병준,Oh, Jung-Hwan,Kwon, Young-Dae,Yoon, Byung-Wook,Choi, Byung-Jun 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.3

DNA 메틸화는 histone modification과 함께 DNA의 염기서열이 유지되면서 유전기능이 변화되고 자손까지 전달 될 수 있는 후생 유전의 중요한 한 부분이다. DNA 메틸화는 크로마틴의 구조를 변경시키는 과정을 통하여 유전자와 repetitive sequence의 표현을 억제시킬 수 있다. DNA 메틸화는 X-불활성화, 유전체 각인, 유전자 발현조절, 암 생성 등에 중요한 역할을 하는 것으로 밝혀졌고, DNA 메틸화 표지자 (DNA methylation marker)들은 종양의 진단과 치료에 대한 반응을 예측하는 지표로 활용되고 있다. 지금까지 많은 연구 성과에도 불구하고 DNA메틸화, 메틸화에 의한 gene silencing, DNA 메틸화의 표적부위 등에 대한 명확한 기전이 아직도 밝혀지지 않고 있어 향후 더 많은 기초적 연구가 필요할 것이다. 최근에는 후생 유전적 변화는 가역적이기 때문에 종양억제유전자를 억압하는 후생 유전적 변화를 제거한다면 그 종양억제유전자를 다시 활성화시킬 수 있다는 개념의 후생유전 치료법 연구로 DNA 메틸화 억제제와 histone deacetyaltion에 관여하는 HDAC의 억제제들이 항암제로서 개발되어 사용되고 있는데 향후 더 많은 약제 개발과 임상적 연구가 진행되어야 할 것이다. Epigenetic is usually referring to heritable traits that do not involve changes to the underlying DNA sequence. DNA methylation is known to serve as cellular memory. and is one of the most important mechanism of epigenetic. DNA methylation is a covalent modification in which the target molecules for methylation in mammalian DNA are cytosine bases in CpG dinucleotides. The 5' position of cytosine is methylated in a reaction catalyzed by DNA methyltransferases; DNMTl, DNMT3a, and DNMT3b. There are two different regions in the context of DNA methylation: CpG poor regions and CpG islands. The intergenic and the intronic region is considered to be CpG poor, and CpG islands are discrete CpG-rich regions which are often found in promoter regions. Normally, CpG poor regions are usually methylated whereas CpG islands are generally hypomethylated. DNA methylation is involved in various biological processes such as tissue-specific gene expression, genomic imprinting, and X chromosome inactivation. In general. cancer cells are characterized by global genomic hypomethylation and focal hypermethylation of CpG islands, which are generally unmethylated in normal cells. Gene silencing by CpG hypermethylation at the promotors of tumor suppressor genes is probably the most common mechanism of tumor suppressor inactivation in cancer.

스포츠와 연관된 과사용 증후군: 주관절

오정환,금정섭,박진영,Oh, Jeong-Hwan,Keum, Jung-Sup,Park, Jin-Young 대한정형외과스포츠의학회 2008 대한정형외과스포츠의학회지 Vol.7 No.2

반복적으로 던지는 동작은 주관절에 심한 기계적인 스트레스를 주며, 주된 기전은 외반력이며 그 결과 내측 인장력과 외측 압박력, 후방부의 충돌현상이 발생한다. 성장기나 청년기에 지속적인 스트레스는 주관절에 해부학적 변화를 유발할 수 있다는 위험성을 운동선수와 가족, 코치들은 반드시 잘 이해하고 있어야 한다. 스포츠와 관련된 주관절 손상을 막기 위하여 적절한 운동법 교육, 워밍업, 의학적 전문 지식, 보호 장구 등이 필요하다. 또한 손상 예방과 재활은 적절한 투구 동작, 근력 강화, 견갑골에 대한 조절, 관절 운동 범위의 적절한 유지가 필수적이다. Repetitive overhead throwing exerts significant mechanical stress on the elbow joint. Pitching in baseball, serving in tennis, spiking in volleyball, passing in American football and launching in javelin-throwing can all produce elbow pathology by forceful valgus stress, with medial stretching, lateral compression and posterior impingement. This stress can lead to developmental anatomic changes in the young thrower. Asymptomatic pathology in the shoulder and elbow joint is prevalent and, with overuse, can progress to disabling injury. Joint injury occurs as a result of the body's inability to properly coordinate motion segments during the pitching delivery, leading to further structural damage. The implications of acute and overuse injuries and the possibility of permanent damage should be understood by parents, coaches and the athletes. Proper understanding of the intrinsic and extrinsic risk factors that could lead to elbow injuries is thus required. Measures to prevent elbow injuries should include proper coaching, warm-up, medical expertise and protective gear. Injury prevention and rehabilitation should center on optimizing pitching mechanics, core strength, scapular control, and joint range of motion.

의치 구내염 환자에서 분리한 Candida albicans의 아크릴 수지에 대한 부착성과 타액 단백질과의 상호 관계

오정환,최부병,최대균,우이형,이성복,권긍록,Oh, Jung-Hwan,Choi, Boo-Byung,Choi, Dae-Gyun,Woo, Yi-Hyung,Lee, Sung-Bok,Kwon, Kung-Rock 대한치과보철학회 1999 대한치과보철학회지 Vol.37 No.5

Adherence of Candida albicans(C. albicans) to the surface of a denture is believed to be an initial and essential step in the formation of denture-induced stematitis. Previous studies have provided enormous infomation on the relationship between composition of palatine gland/parotid saliva and upper denture stomatitis. Relatively little information is available on the correlation between lower denture stomatitis and sublingual-submandibular ( SLSM ) saliva. The plaque samples were collected from the two sites($100mm^2$) on the inner surface of lower partial denture corresponding to the stematitis and healthy region of the lower partial dentures of 12 denture stomatitis patients and 6 nor-mal persons who wore lower partial dentures. The samples were plated to isolate C. albicans on a selective Saboraud's dextrose agar plate and the isolates were identified by germ tube test and gram staining. The subjects were divided into group I (stomatitis with C. albican), group II (lesion without C. albicans), group III (no lesion but C. albicans), and group IV (normal and healthy denture wearer). Individual SLSM saliva($20{\mu}g$ of protein) was analyzed by SDS-PAGE (SDS -poly-acrylamide gel electrophoresis) with Coomassie brilliant blue and PAS(Periodic Acid Schinff) stain-ing. The salivary proteins separated in the polyacryamide gels were subjected to immunoblot anaysis using anti-lactoferrin, anti-sIgA, and anti-secretory component of sIgA. In this study using custom made acrylic denture resin beads(5mm in diameter) coated with stimulated individual SLSM saliva, the binding ability of individual C. albicans strains to the beads was observed. Levels of C, albicans adhered to the acrylic resin beads were determined by measuring the optical density of the bound C. albicans to the beads at 580nm. The results showed that a higher number of C. albicans was observed in the lesion site than healthy site. The saliva of group I contained more high molecular weight glycoprotein(mucin, MGI) as compared to group II, III and IV. And lactoferrin and sIgA affected to the binding ability of C. albicans to acylic resin beads. Binding ability of individual C. albicans to the acrylic resin coated with respective individual saliva was found to be greater in group I than the other 3 groups. And when bound cells of C. albicans isolated from individual subject #2 to the saliva coated beads were used binding ability of subject #2 saliva coated beads was founed to be greater than the other sutjects. These results suggested that denture induced stomatitis is related to individual patient's salivary protein composition, especially MG-1. Future studies will be directed toward saliva exam-ination of patients who have general disease and analysis of pellicles formed on prosthesis with respect to oral disease.

비매듭 금속 봉합 나사못을 이용한 관절경적 방카트 복원술: 임상적 및 방사선학적 결과의 비교

오정환,이상훈,박홍근,전숙하,박준석,김철기,박진영,Oh, Jung-Hwan,Lee, Sang-Hoon,Park, Hong-Keun,Jeon, Suk-Ha,Park, Joon-Suk,Kim, Cheol-Ki,Park, Jin-Young 대한정형외과스포츠의학회 2008 대한정형외과스포츠의학회지 Vol.7 No.2

목적: 비매듭 금속 봉합 나사못(knotless metal suture anchor)을 이용한 관절경적 방카트(Bankart) 복원술의 임상적 및 방사선학적 결과를 비교하였다. 대상 및 방법: 2001년 2월부터 2005년 1월까지 비매듭 봉합 나사못을 이용하여 관절경적 방카트 복원술을 시행하였고 1년 이상 추시 가능했던 68예를 대상으로 하였다. 술 후 평균 추시 기간은 34개월이었다. 평가는 Rowe score, 관절 운동 범위, 주관적 시각 척도(VAS)에 의한 통증 정도를 측정하였으며 수술 전 후 방사선학적 결과와 비교하였다. 결과: Rowe scoring system에서 술 전 43.3에서 술 후 95.6로 평가되었다. 관절운동범위는 최종 추시시 정상 팔에 비해서 거의 차이는 없었다. 주관적 시각 척도에 의한 통증의 정도는 술 전 3.3에서 술 후 0.5로 측정되었다. 방사성 저음영선은 약 15예에서 관찰되었다. 15예 중 2예에서 재탈구 및 나사못 관절병증으로 재수술을 시행하였고 Odds Ratio상 2.6배의 견관절 불안정성을 호소하였다. 결론: 비매듭 봉합 나사못을 이용한 방카트 복원술은 유용한 방법이나 외래 추시 중 나타나는 나사못 주위의 방사성 저음영선은 불량한 예후를 암시할 수 있기 때문에 주의가 필요하겠다. Purpose: To study the clinical and radiologic results with arthroscopic Bankart repair using knotless metal suture anchor. Materials and Methods: From February 2001 to January 2005, 68 patients, who underwent arthroscopic Bankart repair using knotless suture anchor and were followed up more than 12 months, were evaluated. A mean follow-up period was 34 months. All shoulders were evaluated by Rowe scoring system, range of motion of the shoulder, pain degree of VAS, and This was compared by radiologic findings after surgery. Results: The Rowe scoring system was 43.30 preoperatively, which improved to 95.55 postoperatively. At last follow-up, there was no significance difference between operated shoulder and non-operated shoulder in range of motion. The degree of VAS was measured from 3.3 preoperatively to 0.5 postoperatively. The radiolucent line was shown around suture anchor in 15 shoulders. 2 shoulders of 15 shoulders were reoperated due to redislocation and anchor arthropathy. In Odds ratio, this group (15 patients) had more 2.6 times the subjective instability than other group (53 patients). Conclusion: Arthroscopic Bankart repair using knotless anchor suture is very effective operative technique. But we have to be careful because the radiolucent line around anchor showed up during a follow -up period may indicate poor prognosis.

습관적 저작측에 부여한 교합장애로 인한 저작근과 흉쇄유돌근 및 하악운동변화에 관한 연구

오정환,최대균,최부병,Oh, Jung-Hwan,Choi, Dase-Gyun,Choi, Boo-Byung 대한치과보철학회 1995 대한치과보철학회지 Vol.33 No.4

This study was performed to measure the mandibular movement and the changes of masicatory and sternocleidomastoid muscle activity reflected by occlusal disturbance during habitual chewing. For this study, 18 subjects(14 males and 4 females with an average age of 24.0) were selected. The impression of each subject were taken for measuring intermolar distance on lower dentition. The activities of masticatory and sternocleidomastoid muscle and the mandibular movement were recorded and analyzed during habitual chewing by means of E.M.G.(electromyograph), E.G.N.(electrognathograph), rotate program in BioPak analyzing system(BioResearch Inc.). The results were as follows : 1. In EMG of the mandibular rest position, the mean value of muscle activites were increased by nocleidomastoid muscle and anterior belly of digastric muscle(0.05<p<0.1) in working side. 2. During habitual chewing the opening and closing velocities were increased(0.05<p<0.1) after removal of occlusal disturbance and it ws decrase 1week later. 3. During habitual chewing the opening velocities were faster than closing velocities in mandibular movement trace. 4. During habitual chewing the degree of mandibular horizontal rotation is increased(p<0.005) by occlusal disturbance and decreased(0.025<p<0.05) after removal of occlusal disturbance. 5. During habitual chewing mesioanterior-downward translation of non-working side mandibular condyle is more limited in anterior-mesial movement than in inferior-mesial movement caused by occlusal disturbance. 6. During habitual chewing the degree of mandibular frontal rotation is more decreased in the subjects of group function guide than in the subjects of canine guide caused by occlusal disturbance.

인공진피($Terudermis^{(R)}$)와 부분층 피부이식을 이용한 전완피판 공여부 수복

오정환,Oh, Jung-Hwan 대한악안면성형재건외과학회 2007 Maxillofacial Plastic Reconstructive Surgery Vol.29 No.3

The radial forearm fasciocutaneous flap(RFFF) is a well-known flap for the reconstruction of oral and maxillofacial defects. It was first described by Yang et al. in 1981 and Soutar et al. developed it for the reconstruction of intraoral defect. RFFF provides a reliable, thin, and pliable soft tissue/skin paddle that is amenable to sensate reconstruction. It also has a long vascular pedicle that can be anastomosed to any vessel in either the ipsilateral or contralateral neck. However, split thickness skin graft(STSG) is most commonly used to cover the donor site, and a variety of donor site complications have been reported, including delayed healing, swelling of the hand, persistent wrist stiffness, reduced hand strength, and partial loss of the graft with exposure of the forearm flexor tendon. Various methods for donor site repair in addition to STSG have been developed and practiced to minimize both functional and esthetic morbidity, such as direct closure, V-Y closure, full thickness skin graft, tissue expansion, acellular dermal graft. We got a good result of using artificial dermis($Terudermis^{(R)}$) and secondary STSG for the repair of RFFF donor site defect esthetically and report with a review of literature.

인간 게놈의 Copy Number Variation과 유전자 질환

오정환,Oh, Jung-Hwan,Nishimura, Ichiro 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.2

Genetic variation in the human genome occurs on various levels; from the single nucleotide polymorphism to large, microscopically visible chromosome anomalies. It can be present in many forms, including variable number of tandem repeat (VNTRs; e.g., mini- and microsatellites), presence/absence of transposable elements (e.g., Alu elements), single nucleotide polymorphisms, and structural alterations (e.g., copy number variation, segmental duplication, inversion, translocation). Until recently SNPs were thought to be the main source of genetic and phenotypic human variation. However, the use of methods such as array comparative genomic hybridization (array CGH) and fluorescence in situ hybridization (FISH) have revealed the presence of copy number variations(CNVs) ranging from kilobases (kb) to megabases (Mb) in the human genome. There is great interest in the possibility that CNVs playa role in the etiology of common disease such as HIV-1/AIDS, diabetes, autoimmune disease, heart disease and cancer. The discovery of widespread copy number variation in human provides insights into genetic variability among populations and provides a foundation for studies of the contribution of CNVs to evolution and disease. 인간 게놈의 DNA서열의 차이는 개개인의 특이성을 의미하기 때문에 염기서열의 변화는 질병에 대한 감수성, 약물에 대한 반응 등 개인의 성향에 큰 영향을 미치게 된다. 인간 게놈에는 여러 가지 형태의 유전적 변이가 존재하지만 그 중 단일염기다형성이 인간의 유전적, 표현형의 다양성을 설명하는 주된 유전적 변이로 생각되었으나 최근 유전체 전체 분석법의 발전으로 1 kb 이상 크기의 CNV의 발견으로 개체간의 유전적 다양성에 대한 더 많은 이해가 가능하게 되었고, 진화와 유전 질환에 대한 CNV의 역할을 조사하는 연구의 기초를 제공하게 되었다. 현재 인간게놈의 CNV를 찾아내고 특성화 작업을 목표로 하는 The Copy Number Variation Project를 위해 The Wellcome Trust Institute (Hinxton, United Kingdom), Hospital for Sick Children (Toronto), University of Tokyo (Tokyo), Affymetrix (Santa Clara, CA), 그리고 Harvard Medical School/Brigham and Women's Hospital (Boston, MA) 등이 참여하는 international consortium이 구성되어 보다 심도 있는 연구가 진행되고, 또한 향후 진보된 DNA microarray-based technology와 서열화 기술의 개발로 인간 게놈 상의 모든 유전적 변이를 발견하게 되고 포괄적인 CNV 지도를 완성하고 인간 유전자 다양성 인간의 진화, 유전적 질환 개인 맞춤형 의학에 대한 새로운 이해와 연구가 가능하게 될 것으로 기대된다.