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생화학적 표지자을 이용한 근교계 랫드의 유전적 MONITORING
김용준(Yong Joon KIM),현병화(, Byeong Hwa HYUN),김원용(Won Yong KIM),오구택(Goo Taek OH),김환묵(Hwan Mook KIM),최재윤(Jae Yoon CHOI) 한국예방수의학회 1990 예방수의학회지 Vol.14 No.3
A genetic monitoring technique to examine the genetic authenticity of inbred strains of rat was developed and established through biochemical approaches. Four inbred strains, F344/N, LEW /Sea, SHR /Crj and WKYO, were monitored genetically using electrophoresis techniques such as polyacrylamide gel eletrophoresis(PAGE) or cellulose acetate membrane electrophoresis. Results from the present study are as follows; It is very efficient and useful to identify inbred strains of rat for the detection of gene loci of biochemical markers as the genetic monitoring technique. Twenty-three biochemical markers were examined for their gene loci to apply to genetic monitoring, but only a few markers are enough to monitor genetically rat strains for the most case. For genetic monitoring of gene loci which are variable in each substrain, a detection method of major histocompatibility complex (MHC) must be applied together.