만성 간질환 환자의 삶의 질 평가 (논평)

엄상화 ( Sang Hwa Urm ) 대한간학회 2007 Clinical and Molecular Hepatology(대한간학회지) Vol.13 No.1

보건예방사업을 위한 B형간염 표면항체 검사방법 비교

이정녀,엄상화,이종태,전진호,손혜숙,Lee, Jeong-Nyeo,Urm, Sang-Hwa,Lee, Jong-Tae,Chun, Jin-Ho,Sohn, Hae-Sook 대한예방의학회 2000 Journal of Preventive Medicine and Public Health Vol.33 No.2

Objectives : The purpose of this study was to suggest a proper method for the detection of heaptitis B surface antibody(anti-HBs) in a screening program for hepatitis B vaccination. Methods : Sensivitity, specificity and predictive values were compared between Immunochromatographic assay (ICA) and passive hemagglutination(PHA) in 978 subjects(565 males, 413 females, 19-78 years ranging in age, mean 46.5 years old). EIA was used as a standard method for the detection of HBsAb. Results : Sensitivity in the detection of anti-HBs of PHA and ICA was 88.7%, and 94.9%, specificity was 94.3% and 96.6%, negative predictive value was 96.5%, and 98.0%, and positive predictive value was 82.3%, and 91.3%,, respectively. False negative rate(11.3%) of PHA was higher than that(5.1%) of ICA. The higher the titer of anti-HBs in EIA was, the lower the false negative rate was. There was no false negative result in the cases with $101mIU/{\beta}c$ or more in EIA Conclusion : We suggest that ICA should be the choice of screening method in the detection of anti-HBs in Hepatitis B vaccination program.

부산, 울산 및 경상남도 만 19세 남자의 고도근시 유병률

이상준,엄상화,유병철,손혜숙,홍영습,노맹석,이용환,Lee, Sang-Joon,Urm, Sang-Hwa,Yu, Byeng-Chul,Sohn, Hae-Sook,Hong, Young-Seoub,Noh, Maeng-Seok,Lee, Yong-Hwan 대한예방의학회 2011 Journal of Preventive Medicine and Public Health Vol.44 No.1

Objectives: The purpose of this study was to estimate the prevalence and correlated factors of high myopia in 19 year-old men in Southeast Korea. Methods: This retrospective study was based on the medical checkup data of conscription during 2005. The study subjects were 19 years old men in Busan, Ulsan and Gyeongsangnam-do. The health checkup data of the conscripts consisted of noncycloplegic autorefraction test, the biometric data and social factors. To analyze the social and biometric effects, we classified the biometric factors into 4 or 5 groups and the social factors into 3 groups. High myopia was defined as a spherical equivalent of under -6.0 diopter. Data analysis was performed using the chi square test for trends and multiple logistic regression analysis. The SAS(version 9.1) program was used for all the analyses. Results: The prevalence of high myopia was 12.39% (6256 / 50 508). The factors correlated with high myopia were the residence area (OR, 2.07; 95% CI, 1.77 to 2.4 for small city; OR, 2.01; 95% CI, 1.72 to 2.34 for metropolis; the reference group was rural area), academic achievement (OR, 1.43; 95% CI, 1.34 to 1.53 for students of 4-and 6-year-course university; the reference group was high school graduates & under) and blood pressure (OR, 1.54; 95% CI, 1.10 to 2.16 for hypertension; OR, 1.09; 95% CI, 1.02 to 1.17 for prehypertension; OR, 1.10; 95% CI, 1.01 to 1.20 for hypotension; the reference group was normal blood pressure). Conclusions: More than one tenth of the young men were high myopia as one of the risk factor for visual loss. Further studies on high myopia and its complications are needed to improve eye health in Southeast Korea.

남성 사무직 관리자의 생활습관, 스트레스 수준과 과민성 대장증후군

김종렬,엄상화,전진호,정수진,이창희,정귀원,최순석,배기택,Kim, Jong-Ryul,Urm, Sang-Hwa,Chun, Jin-Ho,Jeong, Soo-Jin,Lee, Chang-Hee,Jeong, Kui-Won,Choi, Soon-Seok,Pai, Ki-Taek 대한예방의학회 1997 예방의학회지 Vol.30 No.4

Irritable bowel syndrome(IBS) is one of the common health problem that has been considered as stress-induced. This study was conducted to investigate the relationship between life style and the level of stress and IBS by structured questionnaire which included questions on life style, the self-esteemed gastrointestinal symptoms, and Psychosocial Well-being Index(PWI). Subjects were 1,498 male white collars who get the regular health check and participated in survey at Inje University Health Promotion Center from January to ,December, 1996. The overall prevalence of IBS was 37.5%(561 cases), and the level of stress by PWI score was higher in IBS group$(41.8{\pm}14.2)$ than symptom-free group$(34.6{\pm}12.6)$. As the result of comparison between the two groups, heavier smoking (adjusted O.R=2.48, 95% C.I 1.81-3.41), longer daily working time (adjusted O.R=5.19, 95% C.I 3.59-7.56), stimulatory food materials-mainly hot or salty (adjusted O.R=1.87, 95% C.I 1.44-2.45), higher body mass index (adjusted O.R=1.80, 95% C.I 1.27-2.57), and higher level of stress (adjusted O.R=2.81, 95% C.I 1.80-4.43) were estimated as risk factors of IBS. On the contrary, 6-8 hours sleeping per day (adjusted O.R=0.38 95% C.I 0.21-0.70), 3-4 times exercise per week (adjusted O.R=0.57 95% C.I 0.39-0.83), and tenure more than 20 years (adjusted O.R=0.25 95% C.I 0.16-0.35) were considered as protective factors to IBS. In summary, the assessment of the stress level might be placed in the first priority to control IBS, at least by some degree, which suggested that IBS could be controlled by avoiding such risk factors and by encouraging such protective factors.

임플란트 완성 환자의 구강건강관련 삶의 질과 관련 요인

박정현,엄상화,권현숙,조갑숙,허승주,이소영,유병철,Park, Jung-Hyun,Urm, Sang-Hwa,Kwun, Hyeon-Sook,Cho, Gab-Suk,Heo, Seung-Ju,Lee, So-Young,Yu, Byeng-Chul 한국치위생학회 2011 한국치위생학회지 Vol.10 No.2

Objectives : This study was purposed to estimate the level of oral health quality of life and the relationship between health-related factors and oral health quality of life in patients with successful dental implants. Methods : This questionnaire survey was conducted during the period from December 2009 to March 2010 with 126 patients confirmed successful dental implants in Busan. The oral health related quality of life and oral health-related factors were estimated with OHIP-14 and 8 oral health characteristics, respectively. Data analysis was performed with descriptive analysis, t-test, ANOVA, multiple regression analysis using SAS(ver 9.1) program. Results : The score of oral health quality of life in patients with successful dental implants was $12.25{\pm}7.82$. The scores of subscale of the oral health quality of life were $1.24{\pm}1.35$ in social disability, $1.60{\pm}1.37$ in handicap, $1.60{\pm}1.44$ in psychological disability, $1.70{\pm}1.38$ in functional limitation, $1.81{\pm}1.35$ in physical disability, $2.14{\pm}1.46$ in physical pain, $2.17{\pm}1.46$ in psychological discomfort, respectively. The related factors of oral health quality of life in patients with successful dental implants were absence of other type prosthesis and experience of tooth brushing education. Conclusions : The oral health related quality of life in patients with successful dental implants was relatively good condition. Social supports and chances for high quality denture and tooth brushing educations are needed to improve oral health related quality of life in patients with dental implants.

베트남전 참전자 2세의 건강상태에 관한 조사 - 부산지역 고엽제 위해증 환자를 대상으로 한 설문조사 결과 -

김학준,손혜숙,엄상화,박수경,유병철,이종태,전진호,Kim, Hak-Joon,Sohn, Hae-Sook,Urm, Sang-Hwa,Park, Soo-Kyung,Yu, Byung-Chul,Lee, Jong-Tae,Chun, Jin-Ho 대한예방의학회 2000 Journal of Preventive Medicine and Public Health Vol.33 No.1

Objectives : To propose the referential data to evaluate the health impacts of Vietnam veterans' children whose father were exposed to herbicides in Vietnam War. Methods : Vietnam veterans who visited to Pusan Veteran Hospital for medical care were recruited from April to October, 1998. They were 71 and asked about their own combat history, symptoms and illness, and health status of their 182 children. The informations were collected by direct and phone interview. Exposure estimation was also performed as exposure score depending on year and unit of participation, and personal episodes related to exposure to herbicide in the war. It classified into three groups; lower(<18.0), moderate(18-53), high$(\geq53)$ exposure group. Results : The mean age and the period into the combat of the veterans were 52.8 years and 15.0 months. The mean exposure score was $18.1{\pm}9.9$, and mainly distributed in lower (46.5%) and moderate(52.1%) exposure group. Most(90.1%) of them were diagnosed as sequelae(21 cases) and suspected sequelae(43 cases) of the herbicides by Korean veteran's hospital diagnostic criteria. The major sequelae was peripheral neuropathy 13 cases, chloracne 5 cases, and the major suspected sequelae was hypertension 20 cases, diabetes mellitus 18 cases, liver disease 12 cases, central neuropathy 11 cases, etc. About birth, 42.2% and 16.9% experienced spontaneous abortion and stillbirth, respectively. The mean exposure score was higher in stillbirth experience group(p<0.05). About half of the children(90 cases, 49.5%) hold the abnormal health status: those were skin pigmentation 38 cases, rash 23 cases, congenital anomaly 15 cases, general weakness 12 cases, purpura 8 cases, visual disturbance 8 cases, etc. These health problems had no association with father's exposure level(p>0.05). Conclusions : These results were depend on their own answers, and expectation for compensation did not excluded, therefore, this study may have limitations: inaccuracy of informations due to recall bias and response bias. Nevertheless, through this study, we could image the fundamental aspect for health impacts of Vietnam veterans' children for preparing the national control program and policy. A large scale epidemiologic study with valid exposure assessment on the health impacts of Vietnam veterans' children is recommneded.

VDT 취급 여성 사무직 근로자들의 견관절 기능장애에 대한 운동요법의 효과

최순석,정귀원,엄상화,정수진,이종태,전진호,이채언,배기택,Choi, Soon-Seok,Jeong, Kui-Won,Urm, Sang-Hwa,Jeong, Soo-Jin,Lee, Jong-Tae,Chun, Jin-Ho,Lee, Chae-Un,Pae, Ki-Taek 대한예방의학회 1998 예방의학회지 Vol.31 No.2

Visual display terminal(VDT) is popular in majority of office in modern countries. Musculoskeletal pain due to repetitive work with VDT is a serious industrial accident at present. In recent studies, shoulder pain in VDT users is the most common problem in Korea. The purpose of this study was to evaluate and compare the effect of excercises on shoulder dysfunction in VDT-related female office workers. Of the 119 patients with shoulder dysfunction, 32 patients entered into the study. 16 patients(Group I) were not prescribed excercises, other 16 patients (Group II) performed throughout the specific shoulder excercises such as pendular excercises(for-ward, backward and circumduction) and sidelying external rotation excercises with weights. After 3 months of excercises, the two groups were compared in term of the pain rating scale, disability questionnaire score and range of motion score. The results were summarized as follows ; 1. The pain rating scale in Group I before and after 3 months were not differenced significantly(p value=0.784) and in Group II, the scale before and after excercises were differenced significantly(p value=0.0005). 2. The disability questionnaire scores in Group I before and after 3 months were not differenced significantly(p value=0.437) and in Group II, the scores before and after excercises were differenced significantly(p value=0.0002). 3. The ROM scores of the affected shoulder in Group I before and after 3 months were not differenced significantly(p value=0.876) and in Group II, the scores before and after excercises were differenced significantly(p value=0.0017). In conclusion, that the specific shoulder excercises on shoulder dysfunction in VDT-related female office workers are encouraged.

한국인 폐암환자와 대조군의 CYP2D6 유전적 다형성에 관한 연구

전진호,이창희,엄상화,손병철,박준한,정귀옥,손창학,윤혜경,손춘희,김형인,정진숙,Chun, Jin-Ho,Lee, Chang-Hee,Urm, Sang-Hwa,Son, Byung-Chul,Park, Jun-Han,Jung, Kui-Oak,Sohn, Chang-Hak,Yoon, Hye-Kyoung,Son, Choon-Hee,Kim, Hyung-In,Jeong, J 대한예방의학회 1998 Journal of Preventive Medicine and Public Health Vol.31 No.1

The genetically determined CYP2D6 activity as considered to be associated with cancer susceptibility with inter-individual variation. Genetic polymorphism of CYP2D6(B) and CYP2D6(T) was determined by the two polymerase chain reaction(PCR) and BstN1 and EcoN1 restriction fragment length polymorphisms(RFLP) for 67 lung cancer cases and 95 healthy volunteer controls. The cases were composed of 26 squamous cell carcinoma, 14 small cell carcinoma, 10 adenocarcinoma, 3 large cell undifferentiated carcinoma, and 14 not histologically diagnosed. The results were gained from the 142 subjects (57 cases and 85 controls) who observed successfully in two PCR and BstNl/EcoN1 RELP. Only one and no mutant allele of the CYP2D6(B) and CYP2D6(T) gene was detected, that is, the frequency of mutant allele was very low; 0.7%(1/142) and 0%(0/142), respectively. Detected mutant allele of the CYP2D6(B) was beterozygous type(WM). The odds ratios for lung cancer susceptibility with CYP2D6(B) and CYP2D6(T) genotype were not calculated. These results are similar to the previous understanding that the mutant allele is very rare in Orientals compared to Caucasians, therefore, it considered that CYP2D6(B) and CYP2D6(T) genotypes have maybe no association with lung cancer susceptibility in Koreans. This is the basic data of CYP2D6(B) and CYP2D6(T) genotypes for Koreans. It would be hepful for further study to determine lung cancer susceptibility of Koreans with the data about CYP1A1, CYP2E1, GSTM1 from future study.

건강 진단 수검 여성들의 유방 자가 검진에 대한 인식도 및 실천정도에 관한 연구

정수진,최순석,박준한,엄상화,정귀원,이채언,배기택,Jeong, Soo-Jin,Choi, Soon-Seok,Park, Jun-Han,Urm, Sang-Hwa,Jeong, Kui-Won,Lee, Chae-Un,Bae, Ki-Taek 대한예방의학회 1998 예방의학회지 Vol.31 No.4

The methods for the early diagnosis of breast cancer are mammography, physical examination, breast self-examination(BSE). This study was conducted to investigate the knowledge and practice of BSE by structured questionnaire which included questions on general characteristics, performance of mammography, physical examination of breast, knowledge and practice of BSE. The subjects in this study were 412 females between 20 and 69 year old who visit to the Health Care Center of College of medicine of Inje University from December 1997 to February 1998. The results were as follows. 1. Women who have Soften the regular mammography are 8.19% of these subjects and women who have gotten the regular physical examination by doctors are 4.19% of these subjects. 2. Regarding BSE, 77.4% of these subjects said that they knew BSE and 3.88% of these subjects said that they practiced BSE regularly. It was that women who knew BSE were more younger, better-educated and higher economic status(p=0.001) and lived a normal life with he. husband(p=0.033). And women who practiced BSE regularly were more younger, and married(p=0.001). 3. The most common barrier to doing BSE is 'Do not see the needs/worry unnecessarily', and the most common reasons for doing BSE is 'Early detection of Breast cancer'. 4. The overall frequency and percentage distribution of recommended BSE step in Korea is lower than U.S.A.

The Heterotrimeric Kinesin-2 Family Member KIF3A Directly Binds to Creatine Kinase B

Young Joo Jeong(정영주),Sung Woo Park(박성우),Mi Kyoung Seo(서미경),Sang-Jin Kim(김상진),Won Hee Lee(이원희),Mooseong Kim(김무성),Sang-Hwa Urm(엄상화),Jung Goo Lee(이정구),Dae-Hyun Seog(석대현) 한국생명과학회 2021 생명과학회지 Vol.31 No.3

Kinesin은 세포의 중심부에서 세포막쪽으로 미세소관을 따라 이동하며, heterotrimeric kinesin-2는 kinesin superfamily(KIF)의 한 종류로 미세소관의 plus방향으로 이동하는 분자 모터 단백질이다. Heterotrimeric kinesin-2는 모터 활성을 가지는 3종류(KIF3A, KIF3B와 KIF3C)와 kinesin-associated protein 3 (KAP3)이 결합한 형태로 KIF3s의 운반체 결합 영역을 통하여 다양한 결합 단백질과 결합한다. 그러나, 다양한 운반체를 수송하는 heterotrimeric kinesin-2를 조절하는 조절단백질에 대하여서는 아직 밝혀지지 않았다. 본 연구에서는 heterotrimeric kinesin-2를 조절하는 조절단백질을 단백질을 분리하기 위하여 KIF3A의 운반체 결합 영역과 결합하는 단백질을 효모 two-hybrid system을 사용하여 탐색한 결과, 뇌에 특이적으로 발현하는 세포질 크레아틴 키나아제(CKB)를 분리하였다. CKB의 C-말단은 KIF3A의 운반체 결합 영역과 결합하지만, KIF3B, KIF5B와 KAP3과는 결합하지 않았다. 다른 단백질 키나아제인 CaMKIIa는 KIF3A와 결합하지만 GSK3a는 KIF3A와 결합하지 않았다. 또한 KIF3A은 GST-CKB-C와는 결합하지만 GST-CKB-C와 GST와는 결합하지 않았다. HEK-293T세포에 CKB와 KIF3A을 동시에 발현시켰을 때 두 단백질은 세포 내에서 같은 부위에 존재하며, CKB 혹은 KIF3A을 면역침강한 결과 KIF3A뿐만 아니라 KIF3B와도 같이 침강함을 확인하였다. 이러한 결과들은 CKB-KIF3A 결합은 세포내에서 에너지가 부족되는 조건에서 heterotrimeric kinesin-2의 운반체 수송을 조절할 가능성을 시사한다. Heterotrimeric kinesin-2 is a molecular motor protein of the kinesin superfamily (KIF) that moves along a microtubule plus-end directed motor protein. It consists of three different motor subunits (KIF3A, KIF3B, and KIF3C) and a kinesin-associated protein 3 (KAP3) that form a heterotrimeric complex. Heterotrimeric kinesin-2 interacts with many different binding proteins through the cargo-binding domain of the KIF3s. The activity of heterotrimeric kinesin-2 is regulated to ensure that the cargo is directed to the right place at the right time. How this regulation occurs, however, remains in question. To identify the regulatory proteins for heterotrimeric kinesin-2, we performed yeast two-hybrid screening and found a specific interaction with creatine kinase B (CKB), which is the brain isoform of cytosolic creatine kinase enzyme. CKB bound to the cargo-binding domain of KIF3A but did not interact with the KIF3B, KIF5B, or KAP3 in the yeast two-hybrid assay. The carboxyl (C)-terminal region of CKB is essential for the interaction with KIF3A. Another protein kinase, CaMKIIa, interacted with KIF3A, but GSK3a did not interact with KIF3A in the yeast two-hybrid assay. KIF3A interacted with GST-CKB-C but not with GSK-CKB-N or GST alone. When co-expressed in HEK-293T cells, CKB co-localized with KIF3A and co-immunoprecipitated with KIF3A and KIF3B but not KIF5B. These results suggest that the CKB-KIF3A interaction may regulate the cargo transport of heterotrimeric kinesin-2 under energy-compromised conditions in cells.