일시적 대뇌허혈에 의한 gerbil 해마의 피라밋층에 조직학적 변화

양제훈,고필옥,곽수동,Yang, Je-hoon,Koh, Phil-ok,Kwak, Soo-dong 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

Cardiac arrest, hypoxia, shock or seizure has been known to induce cerebral ischemia. This study was designed to investigate the effect of ischemia on hippocampal pyramidal layer induced by transient bilateral occlusion of the common carotid arteries. Mature Mongolian gerbils were sacrificed at days 2, 4, and 7 after carotid occlusion for 10 minutes. Sham-operated gerbils of control group were subjected to the same protocol except for carotid occlusion. During operation for ischemia, body temperature was maintained $37{\pm}0.5^{\circ}C$ in all gerbils. Paraffin-embedded brain tissue blocks were cut into coronal slices and stained with H-E stain or immunostain by TUNEL method. Neurons with the oval and prominent nucleus and without the eosinophilic cytoplasm in the subfield of hippocamapal pyramidal layer were calculated as to be viable neurons. Their chromatins were condensed or clumped. Their nuclei appeared multiangular or irregularly shrinked. The width of the pyramidal layer was reduced due to the loss of nuclei. At day 2 after reperfusion, some neurons in the CA1 subfield were slightly eosinophilic. But most neurons in the CA2 subfield were strongly eosinophilic. At day 4 day, most neurons in the CA1 subfield were severely damaged and at day 7 day, only a few survived neurons were observed. Survived neurons per longitudinal 1mm sector in the CA1, CA2, CA3, and CA4 subfields of pyramidal layer were investigated. At day 2, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfiedls were 104.5/mm (54.3%), 51.0/mm (33.8%), 105.5/mm (85.6%), and 124.3/mm (93.5%) compared to the nonischemic control group, respectively. At day 4, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfields were 3.2/mm (1.7%), 51.5/mm(34.2%), 95.3/mm (77.4%), and 112.5/mm (84.6%), respectively. At day 7, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfiedls were 0.8/mm (0.4%), 5.7/mm(3.8%), 9.8/mm (8.0%), and 5.0/mm (3.7%), respectively. The mean numbers of apoptotic positive neurons in the CA1 subfield at day 2, 4, and 7 after reperfusion were 67.8/mm, 153.2/mm and 123.7/mm, respectively. These results suggest that the transient cerebral ischemia cause severe damages in most neurons at day 7 and that the prosminent apoptotic positive neurons in hippocampal pyramidal layer are the delayed neuronal death induced by ischemia.

임신우에서 발생된 난포의 기능에 대한 면역조직화학적 관찰

곽수동,고필옥,양제훈,원청길,강정부,Kwak, Soo-Dong,Koh, Phil-Ok,Yang, Je-Hoon,Won, Chung-Kil,Kang, Chung-Boo 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.4

Incidence of estrum or abortions in pregnant cows may be affected by large follicles developed together with corpus luteum in pair ovaries of pregnant cows. But the follicles of pregnant phase were not assessed about histological findings. Determination of the healthy and atretic follicles by presence of proliferative cells or apoptotic cells and histological compositions of follicles would be used as important data on measurements of ovarian functions. This study was focussed mainly to investigate macroscopical, histological and immunohistochemical findings of ovarian follicles of pregnant Korean native cows and dairy cows (Holstein). In immunohistochemical methods, assessments of proliferative cells using PCNA antibody and apoptotic cells using TUNEL methods were performed. The follicles were observed on all 24 pregnant cows (17 Korean native cows and 7 Holstein cows). Follicles of greater than 10 mm in daimeter were developed in 37.5% (9/24 heads) of these pregnant cows. largest follicles from in these cows were $16.0{\times}15.0mm$ in diameter in a Korean native cow(l20 days of gestation), $13.4{\times}10.1mm$ in a Korean native cow(50 days of gestation), $12.9{\times}11.5mm$ in a Holstein cow (120 days of gestation). 40.5% among all follicles having diameter of greater than 1.0 mm in pregnant cows were assessed as atretic follicles and in addition, healthy follicles also showed less in number and smaller in size and thinner in wall layer compared with those of cyclic phase ovaries. In immunohistochemical findings, also proliferative positive cells and apoptotic positive cells on the granulosa cell layers in the healthy follicles of pregnant cows appeared less than on those of cyclic follicles. So these follicles were assessed as weakly active follicles. In large follicles, above positive cells were not nearly appeared but granulosa cell debris were more appeared among the granulosa cells. So these large follicles were assessed as inactvie or atretic follicles. The above findings suggest that small follicles of pregnant phase were weakly active or atretic and large follicles were inactive or atretic.

한우의 임신경과에 따른 황체조직의 광학 및 전자현미경적 변화

표병민,고필옥,양제훈,원청길,조규완,강정부,곽수동,Pyo, Byong-min,Koh, Phil-ok,Yang, Je-hoon,Won, Chung-kil,Cho, Gyu-wan,Kang, Chung-boo,Kwak, Soo-dong 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.3

Corpus luteum (CL) is the primary productive organ of progesterone in pregnant cows. Progesterone levels in bovine plasma depend on the volume, weight and shape of the CL. Progesterone productions during the late stages of gestation occur both in the CL and placenta, and placentas producted more progesterone than CL on progesterone prcduction. Because division of progesterone production of these two organs is impoxxible, the CL function can not be determined by plasma progesterone levels following gestation stages. This study was carried out to evaluate histological findings on the CL spurium and CL verum, and also on the CL following the pregnant stages by histological and immunohistochemical and electron microscopical methods and then we expect to assume the functions of CL by histological findings. 1. Proliferations of luteal cells occur by day 120 of gestation, vessel hyperplasia occur by day 90 of gestation, and the walls and lumens of vessels developed by day 120 of pregnancy. 2. Sizes of CL cells increased to maximum around day 200 of gestation and similarly maintained by day 240. So these findings indicated that the function of Cl is most active around day 200 of gestation. 3. On parturation day, the number and size of luteal cells were maintained but stain intensity of the luteal cells and vessels are declined or disappeared, and fibrosis of luteal cells increased, and the vessel lumens are emptied. These findings indicate that CL is inactive. 4. In immunohistochemical findings, proliferative positive cells by PCNA antibody appeared more in number during early stages of gestation but appeared less following course of pregnant stages and not nearly appeared on day 120 of gestation. Apoptotic positive cells by TUNEL methods not nearly appeared on the early pregnant stages and a few appeared at late pregnant stages. So developments of CL proceed until day 120 of gestation and regression of CL was occurred by transform of luteal cells into fibrocytes than by luteal cell apoptosis. 5. In electron microscopical findings, the size of luteal cells increased more in CL verum than in CL spurium. During gestation stages, the size of luteal cells increased, mitochondria in the luteal cell cytoplasms densely and abundantly developed and also swelled mitochondria increased. The interspace of luteal cells are also dilated, transformation of luteal cells into fibrocytes are more number. The lumens and walls of peripheral capillaries of large luteal cells more broadened and thickened, and transformation of large and small luteal cells to fibrocytes are increased. The above findings suggest that function of pregnant CL more developed by day 120 of gestation and are most active around day 200 of gestation and similarly maintained by day 240 and are promptly regressed on paturation day.

말의 흉추골유합과 요추골변형의 1례

김종섭,송치원,조규현,이상래,양제훈,원청길,Kim, Chong-Sup,Song, Chi-Won,Cho, Gyu-Hyen,Lee, Sang-Rae,Yang, Je-Hoon,Won, Chung-Kil 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1

The fused thoracic vertebra and deformity of the lumbar vertebrae in the female Thoroughbred horse were observed macroscopically. The 11th and 12th thoracic vertebrae (T) were partially fused. They were composed of three parts between the spinous process of the 11th and 12th T, left and right caudal articular processes of the 11th T, and left and right cranial articular processes of the 12th T. The vertical surface of cranial articular process of the 11th T and left mamillary process of the 12th T were absent. The left caudal part of the transverse process of the 12th T and left costal fovea of the transverse process of the 12th T were severely deformed. On the other hand, the left transverse process of the first lumbar vertebra (L) was a typical rib-like transverse process and two times longer than the right transverse process of the 2nd L. The right transverse process of 4th L has an oval concave facet on the medial part of caudal border for articulation with the right transverse process of 5th L; the latter has a corresponding convex facet on the medial part of the cranial border in the right transverse process of the 5th L.

Nitric Oxide의 만성적인 억제로 인한 고혈압성 SD 랫드에서 Insulin 및 전해질의 변화

배형준(Hyung-Joon Bae),김길수(Kil-Soo Kim),양제훈(Je-Hoon Yang),진복희(Bok-Hee Jin) 한국실험동물학회 1999 Laboratory Animal Research Vol.15 No.2

Gerbil 해마에서 일시적 대뇌허혈에 의한 신경세포의 Apotosis에 미치는 Zinc의 효과에 관한 면역조직학 및 전자현미경적 연구

임도선 ( Do Seon Lim ),배형준 ( Hyung Joon Bae ),김태전 ( Tae Jeon Kim ),강희규 ( Hee-gyu Kang ),양제훈 ( Je Hoon Yang ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

Zinc is one of the trace elements playing an important role in many fundamental biological process. The divalent cation zinc has been reported to possess several physiological properties such as blocking apoptotic cell death through an inhibitory effect on Ca²+, Mg²+ endonuclease activity, or modulating the neurotoxicity via glutamate receptor subtypes. In the present study, we investigated the effect of peripherally injected zinc on delayed neuronal cell death seen in the hippocampus after transient global ischemia, in order to elucidate a possible beneficial role on zinc in ischemic neuronal cell death. Forty adult Mongolian gerbils of male underwent transient bilateral clipping the common carotid arteries for 5 min. In the pretreated animals, ZnCl₂(20mg/kg) was injected subcutaneously once, 1hr before ischemia. Histological survey was carried out 4 days later by in situ DNA fragmentation method and hematoxylin-eosin staining by semiquantatively counting dead neurons in the CA1 sector. And ultrastructural study was also undertaken to determine the effect of zinc on the evolution of transient global ischemia-induced neuronal injury in the gerbil hippocampus. In histological and ultrastructural study, pretreatment of zinc significantly reduced the nuclear damage and subsequent neuronal death against ischemic damage. The result of the present study suggest that the pretreatment of zinc might be an effective tool for the treatment of cerebral ischemia.