The Inhibitory Effects of Ahnjeonbaekho-tang on FRTL-5 Cell Proliferation and Thyroxine Synthesis

안세영,Kang, Shin-Ik,Lee, Byung-Cheol,Ahn, Young-Min,Doo, Ho-Kyung,Ahn, Se-Young 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.3

Objective : Graves' disease, the most common cause of hyperthyroidism, is an autoimmune disorder associated with autoantibodies to the TSH receptor. The clinical features of Graves' disease are goiter and hypermetabolic symptoms induced by excessive hormones. Antithyroid drug therapy is the first-line treatment for Graves' disease in Korea, Japan and European countries. Yet in spite of a long period and high-dose of treatment, it is hard to achieve remission because of adverse effects, frequent recurrence and resistance to antithyroid drugs. Recently, it has been reported that the abnormal thyroid hormone and clinical symptoms of Graves' disease were reduced by Ahnjeonbaekho-tang (AJBHT). Methods : To investigate the effectiveness and action mechanism of AJBHT, we studied the influence of AJBHT on FRTL-5 thyroid cell proliferation, DNA synthesis and expression of T4, TSH, cAMP, Tg and TPO mRNA. Results : AJBHT significantly inhibited the FRTL-5 cell proliferation, DNA synthesis, T4 synthesis, cAMP production and the expression of Tg mRNA in comparison with control and MMI. Conclusions : These results suggest that AJBHT may inhibit the cell proliferation and DNA synthesis by regulating the cAMP, and suppress the T4 synthesis by modulating Tg mRNA expression and cAMP synthesis, and that it may be useful agent for treating the goiter and hormone abnormality of Graves' disease.

堤大川 流域 畓用水의 反復利用

안세영,이근후,Ahn, Se-Young,Lee, Keun-Hoo 한국농공학회 1991 韓國農工學會誌 : 전원과 자원 Vol.33 No.3

To investigate the status of repeated use of irrigation water observations of irrigation water were made on dry days during the irrigation periods in 1986 and 1987 crop year. The total area of studied site is 1,441 ha. The site is a major portion of Jedaecheon basin which is located in Bubuk-myeon, Miryang-gun, Gyeongnam Province. The studied area was subdivided into six small blocks. The inflow and outflow of daily irrigation water for these subdivided blocks were checked. Obtained results are as follows : 1. The over all possible ratio of the repeated use of irrigation water in studied area was 48.3%, which means that approximately one half of the surface flow was possible to be repeatedly used as irrigation water in this area. 2. The ratio of repeated use of irrigation water in sloppy paddy area was 4.29%, whereas, the ratio was 21.97% in the plain area. The average figure of this ratio over studied area was estimated as 17.43%.

리튬이차전지 난연성 전해액 첨가제로서의 Tris(2,4,6-trimethoxyphenyl)Phosphine의 열적, 전기화학적 특성

안세영,김기택,김현수,Ahn, Se-Young,Kim, Ke-Tack,Kim, Hyun-Soo 한국전기전자재료학회 2008 전기전자재료 Vol.21 No.12

Thermal and electrochemical properties were discussed with tris(2,4,6-trimethoxyphenyl)Phosphine (TTMPP) as a flame retradant additive for Li-ion battery. TTMPP showed excellent thermal stability with charged cathodes. Addition of 1 wt.% of the additive to the electrolyte improved the thermal stability without damaging the performance of the battery. The oxygne evolution reaction delayed nearly by $60^{\circ}C$. The capacity retention ratio in cycle life tests of the battery with 1 wt.% TTMPP was slightly improved comparing to the no additive cells.

리튬이온전지용 난연성 첨가제(TCP, TFPP)의 전기화학적 특성

안세영,김기택,김현수,남상용,Ahn, Se-Young,Kim, Ke-Tack,Kim, Hyun-Soo,Nam, Sang-Yong 한국전기전자재료학회 2007 전기전자재료학회논문지 Vol.20 No.9

Flame retardant(FR) properties were investigated with tricredyl phosphate(TCP) and tris(pentafluorophenyl)phosphine(TFPP) as additives for lithium-ion batteries. Thermal stability was improved with additives in $Li/LiNi\frac{1}{3}Mn\frac{1}{3}Co\frac{1}{3}O_2$ cells comparing to non-additive electrolytes. Oxygen evolution reaction of the cathode material was delayed to up $55^{\circ}C$, from $275^{\circ}C\;to\;330^{\circ}C$. Electrolytes with the 1 wt.% additives provided good FR properties while the resonable battery performance is maintained.

리튬이차전지 난연성 전해액 첨가제로서의 Tris(2,4,6-trimethoxyphenyl)Phosphine의 열적, 전기화학적 특성

안세영,김기택,김현수,Ahn, Se-Young,Kim, Ke-Tack,Kim, Hyun-Soo 한국전기전자재료학회 2008 전기전자재료학회논문지 Vol.21 No.12

Thermal and electrochemical properties were discussed with tris(2,4,6-trimethoxyphenyl)Phosphine (TTMPP) as a flame retradant additive for Li-ion battery. TTMPP showed excellent thermal stability with charged cathodes. Addition of 1 wt.% of the additive to the electrolyte improved the thermal stability without damaging the performance of the battery. The oxygne evolution reaction delayed nearly by $60^{\circ}C$. The capacity retention ratio in cycle life tests of the battery with 1 wt.% TTMPP was slightly improved comparing to the no additive cells.

제대천(堤大川) 유역(流域) 답지대(畓地帶)의 물수지(收支)

안세영 ( Ahn Se Young ),이근후 ( Lee Keun Hoo ) 한국농공학회 1990 韓國農工學會誌 : 전원과 자원 Vol.32 No.3

To investigate the status of irrigation water use and the degree of repeated use of irrigation water, observations for water balance analysis were made during the irrigation periods in 1986 and 1987 crop year. The total area of studied site is 1,441 ha. The site is a major portion of Jedaecheon basin which is located in Bubuk-myeon, Miryang-gun, Gyeongnam Province. The studied area was subdivided into six small blocks. The water balance analysis for these subdivided blocks were carried out considering characteristics of each block. Obtained results are as follow: 1. In mountainous sloppy paddy area(less than 7% slope), the surface inflow was 5A mm/day in average that is one third of the surface inflow into plain paddy area ; 16.7 mm/day. 2. The surface inflows at the vegetative stage and the ripening stage were 15.5 mm/day and 10.4 mm/day, respectively. Those figures were larger than the actual consumptive use at respective same stages ; 13.3 mm/day and 9.2 mm/day, respectively. Whereas, the surface inflow at generative stage was 12.5 mm/day which was less than 14.0 mm/day ; the actual consumptive use. 3. The range of the variation of water storage term was 1 mm/day. This means that there were no change in depth of ponded water on paddy fields. The relationship between the variation of water storage(△S) and the variation of ground water table(H) could be expressed as follow: : △S=0.14H+0.26 4. The ground water inflow: into the transition region ; paddy fields which are located continuously from the mountainous area to the plain area, was larger than the out flow from this region, in general. Rowever, in the plain region where the ground water utilization was predominant, the ground water outflow from this region was larger than inflow: to this region. The relationship between the ground water flow(G₂- G₁) and the consumptive use in large paddy area(D₁-D₂) could be expressed as follow: (G₂-G₁) =0.95(D₁-D₂) -3.79.

베타세포 손상에 대한 오배자 추출물의 세포보호작용

안세영(Se-Young Ahn),김용석(Yong-Seok Kim),두호경(Ho-Kyung Doo),박인선(In-Sun Park),성제경(Je-Kyeong Sung),심영준(Young-Jun Shim),민본홍(Bon-Hong Min) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.3

오배자의 수용성 추출물은 alpha-glucosidase의 활성억제를 통해 장관에서의 탄수화물 분해를 방해하여 포도당흡수를 억제 함으로서 식후 혈당농도 (postprandial blood glucose)를 낮추는 효과가 있음이 보고된 바 있다(Shim et al., 2003). 이에 따라 본 연구에서는 오배자 추출물이 베타세포에 대한 보호작용을 함으로 항 당뇨효과를 나타낼 수 있는지를 관찰하고자 하였다. 이를 위하여 흰쥐 (Sprague-Dawley rat)에 오배자 추출물을 지속적으로 투여하는 동안 streptozotocin (STZ)으로 당 뇨병을 유도한 후 이자조직과 혈당의 변화를 관찰하였다. STZ에 의한 당뇨유발은 신생쥐에 STZ을 투여하는 모델 (신생쥐 /STZ)과 osmotic pump에 의해 일정량의 STZ이 지속적으로 투여되는 방법을 이용하였다. 신생쥐/STZ 모델에서 오배자를 투여하지 않은 대조군에서는 외분비 선포조직의 일부 소엽에서 완연한 염증반응과 조직퇴화현상이 관찰되었으며 심한 림 프세포의 침윤이 관찰되었다. 특히 이러한 염증소엽의 이자섬 (islet)들은 매우 작아져 있거나 몇 개의 세포무리로 구성된 경우도 있었다. 특히 염증이 없는 이자소엽에 존재하는 일부 이자섬들에서도 림프구침윤에 의한 인슐린염증현상을 비롯 하여 베타세포의 손상에 의한 조직변형이 나타났다. 이에 비하여 오배자를 투여한 동물의 이자조직은 전술한 바와 같은 염증성 이자소엽은 거의 관찰되지 않았으며, 이자섬에서도 염증반응이 발견되지 않았다. 이때, 대부분 이자섬들이 중심부 베타세포-주변부 알파세포의 세포배열과 정상 크기를 유지하고 있었다. Osmotic pump를 실험동물의 피하에 이식하여 STZ (15 mg/kg/day)을 48시간 동안 일정한 농도로 투여한 당뇨병모델에서는 오배자를 투여하지 않은 경우 대부분 동물들 이 고혈당 (¤200 mg/dl)을 나타냈다. 이들의 이자의 조직소견은 대체로 신생쥐/STZ모델과 유사 하였으나 일부 이자섬 조 직들에서 나타나는 인슐린염증의 정도가 더 심한 것이 많았으며, 알파세포의 이상증식에 의한 소위 ‘변형이자섬(remodeled islet)’ 또는 ‘거대신생이자 섬 (giant neogenic islet)’이 관찰되었다. 이 모델에서 오배자를 투여한 실험동물의 이자조직에서는 변형된 이자섬 및 거대신생이자 섬의 출현이 드물었으며, 고혈당증이 지연되어 나타났다. 이와 같은 결과들은 오배자 추 출물이 식후혈당을 조절함으로 베타세포에 대한 당독성 (glucotoxicity)을 낮출 뿐 아니라 항염증작용에 의한 베타세포보호 작용이 있음을 시사하는 것이다. We have previously reported that aqueous extract of gall from Rhus chinensis, known as “Obaeja”, inhibited rat intestinal alpha-glucosidase and suppressed postprandial hyperglycemia by delaying digestion and absorption of intestinal carbohydrate (Shim et al., 2003). This led us to speculate that obaeja could be involved in ameliorating beta-cell injury by lowering glucotoxicity. In the present study, we thus examined the protective effect of obaeja on pancreatic beta-cell damage along with its anti-diabetic effect in streptozotocin-induced animal models. Streptozotocin was administered to rat pups (neonate/STZ model), or to adult rats with a lower dose using osmotic pump (osmotic pump/STZ model) for inducing beta cell death and diabetes. Obaeja was given to those rat pups after weaning in neonate/STZ model, or 2 weeks before subcutaneous implantation of osmotic pump to rats of the other latter model. In the diabetic control rats of the neonate/STZ model, which were not fed with obaeja, some pancreatic islets demonstrated a destruction of beta cell mass with insulitis 2 weeks after weaning, while some larger and irregular islets were formed by proliferation of alpha cells. In particular, we found some pancreatic lobules showing a severe inflammation and degeneration of islet and acinar tissues in this model. Islets in these inflammatory lobules were smaller in size with only few cells. In contrast, any inflammatory responses and insulitis were not observed in pancreas of the rats fed obaeja in this model. The islets in those rats maintained their normal profiles and islet cell population. Such anti-cytotoxic effect was also monitored in the diabetic rats of osmotic pump/STZ model. Especially, occurrence of hyperglycemia in the obaeja fed rats was delayed by 25~30 days than that of diabetic control rats in this model. Taken together, these results imply that regulation of postprandial blood glucose level by obaeja feeding may ameliorate a secondary injury caused by glucotoxicity.

국제통상론적 측면에서 본 한 불 톰슨민영화 마찰

안세영 (Se Young Ahn) 한국EU학회 1997 EU학연구 Vol.2 No.1

N/A

사이버무역시대 전자상거래의 국제통상이슈에 대한 고찰

안세영(Se Young Ahn) 한국국제통상학회 1999 국제통상연구 Vol.4 No.2

Rapid progress in electronic commerce and cyber trade, which symbolize the digital economy in the 21st century, is fundamentally changing the competition paradigm of the global economy. Accordingly, advanced countries, including the U. S. and EU are making various efforts to secure comparative advantage in the context of international negotiation on the so-called `Cyber Round.` They are actively promoting the early establishment of seven international rules on electronic commerce, which are Non-tariff, Non-taxation, Consumer Protection, Contents Regulation, Encryption, Privacy Protection and Protection of IPR. These new international trade rules will have a great impact on Korea`s perspectives on trade as new rules of cyber trade, which will account for a considerable share of the global economy in the future. Therefore, unlike in the Uruguay Round, Korea should actively participate in the Cyber Round negotiations. The most important task for efficient trade negotiations is to clearly define the `trade interests.` that Korea should pursue. However, cyber trade interests are difficult to define due to cyber trade`s speed of development, unpredictability and the dual characteristics of the Korean economy as an intermediary country. Therefore, the top priority for Korea`s negotiation strategies is to clearly define cyber trade interests and to carry out aggressive negotiations to reflect them in the international arena. Unlike bilateral negotiations, the Cyber Round of negotiations proceeds within a multilateral framework which does not specify negotiating partners. As a result, participating countries might easily find themselves in a so-called Soft Positional Negotiation Game, where they follow opinions of some `invisible leading countries.` Therefore, Korea should pursue Principled Negotiation Game to realize its cyber trade interests as an intermediary country squeezed between advanced countries which advocate market-driven principle and developing countries which argue for more government intervention.

특집 : 음위의 한의학적 개념과 치료법 ( Special Edition : Basic Concepts and Treatment Methods of Impotence in Oriental Medicine )

안세영(Se Young Ahn) 경희대학교 경희의료원 1996 慶熙醫學 Vol.12 No.1