복강내 NF-κB decoy oligodeoxynucleotide를 이용한 수술 후 복강내 유착 발생 감소

안별님 ( Byul Nim Ahn,),송미현 ( Mi Hyun Song,),김주현 ( Ju Hyun Kim,),김경현 ( Kyung Hyun Kim ),박관규 ( Kwan Kyu Park ),최윤석 ( Youn Seok Choi ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.4

목적 쥐모델에서 nuclear factor κB decoy oligodeoxynucleotide (NF-κB decoy ODN) 복강내 주입을 이용한 수술 후 복강내 유착 발생 억제 효과를 알아보고자 하였다. 연구방법 40마리의 BALB/c 암컷 생쥐를 ketamine과 xylazine 복강내 주사를 이용하여 마취 후 개복하고 cytobrush를 이용하여 복막이 벗겨지는 상처를 입히고, 실험군은(n = 20) NF-κB decoy ODN (10 μg, 500 μL)을 복강내에 채우고, 대조군은(n=20) 생리 식염수 500 μL를 복강내에 채운 후 봉합하고 2주 뒤에 유착 정도를 판정하였다. 유착 정도는 유착이 없는 0등급에서 심한 정도에 따라 경증, 중등도, 중증 등급(1-3등급)으로 분류하였다. 결과 수술 후 첫날 실험군에서 2마리, 대조군에서 3마리의 생쥐가 사망하였다. 대조군은 경증과 중등도의 유착이 각각 2마리에서 관찰되었고 13마리에서 중증의 유착이 관찰되었으나, 실험군에서 4마리는 유착이 관찰되지 않았고, 6마리는 경증의 유착, 8마리는 중등도의 유착이 관찰되어 통계적으로 유의한 차이를 나타내었다(P<0.001). 결론 NF-κB decoy ODN를 복강내에 주입하여 NF-κB 작용을 차단하는 방법은 수술 후 복강내 유착 정도를 줄일 수 있다. Objective The objective of this study was to evaluate the effect of intra-peritoneal nuclear factor κB (NF-κB) decoy oligodeoxynucleotide on the prevention of postoperative intra-abdominal adhesion. Methods Forty female BALB/c mice were randomly assigned into 2 groups. The mice were anesthetized and then midline abdominal incision was made. Peritoneal injury was given using cytobrush until deperitonealization. The experimental group (n = 20) received NF-κB decoy oligodeoxynucleotide (NF-κB decoy ODN) in the peritoneal cavity before abdominal closure, while the control group (n = 20) received saline. At 2 weeks postoperation, after the mice were euthanized, the status of intra-abdominal adhesion was evaluated. The degrees of adhesion were graded into no (grade 0), mild (grade 1), moderate (grade 2), and severe adhesion (grade 3). Results Two mice in the experimental group and 3 mice in the control group were dead within 1 day postoperation. In the control group, 2, 2, and 13 mice showed mild, moderate, and severe adhesion, respectively. In the experimental group, 4 mice showed no adhesion, whereas, 6 and 8 mice showed mild and moderate adhesion, respectively. The difference of degrees of adhesion between the groups was statistically signifi cant ( P<0.001). Conclusion Intra-peritoneal NF-κB decoy ODN reduced the severity of postoperative intra-abdominal adhesion.

복강경 수술을 이용한 XY 부분 생식샘 발생장애 환자의 서혜부관내 선형 생식샘제거술

송미현 ( Mi Hyun Song ),안별님 ( Byul Nim Ahn ),김혜경 ( Hae Kyung Kim ),오훈규 ( Hoon Kyu Oh ),이태성 ( Tae Sung Lee ),최윤석 ( Youn Seok Choi ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.3

A 33-year-old phenotypic female presented with primary amenorrhea. The physical examination revealed no breast development, scanty axillary and pubic hair, normal female external genitalia, and a short blind vagina. The findings of magnetic resonance imaging, karyotyping, and hormonal examinations supported the diagnosis of XY partial gonadal dysgenesis. Laparoscopic surgery showed that spermatic cords, including the bilateral streak gonads and epididymes, were within the inguinal canals. After opening the spermatic cords and identifying the lower borders of the streak gonads, the spermatic cords were transected at the level of the vas deferens. A prophylactic herniorrhaphy using polyprophylene mesh was performed due to weakening of the internal inguinal ring. Pathologic evaluation of the gonads confi rmed the diagnosis of partial gonadal dysgenesis without evidence of malignancy. A laparoscopic approach is feasible for treatment of XY gonadal dysgenesis, even if the streak gonads are present within the inguinal canal.

Sargassum sp. Attenuates Oxidative Stress and Suppresses Lipid Accumulation in vitro

Jung-Ae Kim(김정애),Fatih Karadeniz,Byul-Nim Ahn(안별님),Myeong Sook Kwon(권명숙),Ok-Ju Mun(문옥주),Mihyang Kim(김미향),Sang-Hyeon Lee(이상현),Ki Hwan Yu(유기환),Yuck Yong Kim(김육용),Chang-Suk Kong(공창숙) 한국생명과학회 2014 생명과학회지 Vol.24 No.3

인간 생체 내 산화스트레스는 조직적 손상을 일으켜 당뇨병, 심장혈관계질환, 비만 등의 대사성 질환의 발병에 관여하는 것으로 알려져 있다. 본 연구에서는 질병예방용 천연 보조제 개발의 일환으로 모자반 3종류(Sargassum hemiphyllum, Sargassum thunbergii, Sargassum honeri)의 에탄올 추출물을 이용하여 항산화활성 및 지방생성억제효과를 비교 검토하였다. 항산화활성은 DPPH 라디칼 소거능, 세포 내 ROS 활성 및 NO 함량의 측정을 통해 검토하였다. 지방세포생성억제활성은 3T3L1세포를 이용하여 지방세포의 축적 정도와 PPARγ 유전자의 발현 정도를 각각 Oil-Red O 염색법과 RT-PCR로 측정하였다. DPPH 라디칼 소거능 결과, 모자반의 처리에 의해 농도 의존적으로 DPPH 라디칼이 줄어드는 경향을 보였으며, 모자반 중에서 S. hemiphyllum 처리군에서 가장 높은 소거 효과를 볼 수 있었다. MTT assay을 통해 모자반의 에탄올 추출물들이 RAW 264.7 cell에 대한 독성을 보이지 않는 농도에서 세포내 실험을 진행하였다. 세포 내 ROS 소거능의 측정 결과, 농도 의존적으로 DCF 형광도 값이 낮게 나왔으며, 시간이 지남에 따라 형광도 값이 일정하여 모자반의 에탄올 추출물에는 세포 내 ROS 생성을 억제하는 효과가 있음을 알 수 있었다. LPS 처리에 의해 증가한 NO 값은 모자반의 추출물들의 처리에 의해 유의적으로 감소하였으며, 감소 정도는 S. hemiphyllum과 S. thunbergii 처리 군에서 높게 나타났다. 모자반의 추출물들을 3T3-L1지방세포에 유도물질과 함께 처리한 결과 모자반 추출물 중 S. hemiphyllum과 S. thunbergii이 세포 내 지방 축적 및 PPAR γ 유전자의 발현을 유의적으로 감소시키는 것으로 나타났다. 이상의 결과로부터 모자반 추출물들 중 S. hemiphyllum과 S. thunbergii이 높은 항산화 활성과 지방세포 생성억제 효능을 보유하고 있는 천연소재임을 확인할 수 있었다. Oxidative stress causes tissue damage and facilitates the progression of metabolic diseases, including diabetes, cardiovascular heart diseases, and obesity. Lipid accumulation and obesity-related complications have been observed in the presence of extensive oxidative stress. As part of an ongoing study to develop therapeutic supplements, Sargassum sp. were tested for their ability to scavenge free radicals and intracellular reactive oxygen species (ROS), as well as to suppress lipid accumulation. Three species, S. hemiphyllum, S. thunbergii, and Sargassum horneri, were shown to scavenge free radicals in a di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assay. In addition, Sargassum sp. was shown to scavenge intracellular ROS and to decrease nitric oxide (NO) production in H₂O₂ and lipopolysaccharide (LPS)-induced in RAW264.7 mouse macrophages, respectively. Taken together, the results suggest that Sargassum sp. possess huge potential to relieve oxidative stress and related complications, as well as lipid-induced oxidation. They indicate that S. hemiphyllum, S. thunbergii, and S. horneri are potent functional supplements that can produce beneficial health effects through antioxidant and antiobesity activities, with S. hemiphyllum being the most potent among the Sargassum sp. tested. A potential mechanism for the effect of Sargassum sp. on the suppression of lipid accumulation in differentiating 3T3-L1 mouse preadipocytes through deactivation of the peroxisome proliferator-activated receptor γ (PPAR γ) is presented.

Lactobacillus casei로 발효한 톳 추출물의 항염증 활성

문옥주(Ok-Ju Mun),권명숙(Myeong Sook Kwon),배민주(Min Joo Bae),안별님(Byul-Nim Ahn),Fatih Karadeniz,김미향(Mihyang Kim),이상현(Sang-Hyeon Lee),유기환(Ki Hwan Yu),김육용(Yuck Yong Kim),서영완(Youngwan Seo),공창숙(Chang-Suk Kong) 한국생물공학회 2015 KSBB Journal Vol.30 No.1

In this study, we investigated the anti-inflammatory effects of fermented Hizikia fusiformis extracts in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. The fermentation was performed using Lactobacillus casei in mixture of carbon source at 30℃ for 30 days. The sample groups were prepared with/without L. casei group in order to demonstrate the anti-inflammatory activity of fermented H. fusiformis in regard to lactic acid bacteria. As a result, we confirmed the inhibitory effect of H. fusiformis extracts on LPS-stimulated NO production and expression of TNFα, while it had no regulatory effect on the expression of iNOS, COX-2, IL-1β and IL-6 as important inflammatory factors. However, L. casei fermented group significantly suppressed the expression of the above factors. In particular, the difference between the two groups in the matter of mRNA expression of iNOS, which is directly associated with NO production, indicated that the fermentation with lactic acid bacteria effectively suppressed NO production by regulating iNOS expression. Also, effective suppression of pro-inflammatory cytokines showed that the fermentation using L. casei may provide an increment towards extraction of active ingredients that are effective anti-inflammatory agents.