흰쥐에서 체감각유발장전위의 기록부위별 특성과 경로분석

신현철,박용구,이배환,류재욱,조춘식,정상섭,Shin, Hyun Chul,Park, Yong Gou,Lee, Bae Hwan,Ryou, Jae Wook,Zhao, Chun Zhi,Chung, Sang Sup 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.7

Objective : Somatosensory evoked potentials(SSEPs) have been used widely both experimentally and clinically to monitor the function of central nervous system and peripheral nervous system. Studies of SSEPs have reported the various recording techniques and patterns of SSEP. The previous SSEP studies used scalp recording electrodes, showed mean vector potentials which included relatively constant brainstem potentials(far-field potentials) and unstable thalamocortical pathway potentials(near-field potentials). Even in invasive SSEP recording methods, thalamocortical potentials were variable according to the kinds, depths, and distance of two electrodes. So they were regarded improper method for monitoring of upper level of brainstem. The present study was conducted to investigate the characteristics of somatosensory evoked field potentials(SSEFPs) of the cerebral cortex that evoked by hindlimb stimulation using ball electrode and the pathways of SSEFP by recording the potentials simultaneously in the cortex, VPL nucleus of thalamus, and nucleus gracilis. Methods : In the first experiment, a specially designed recording electrode was inserted into the cerebral cortex perpendicular to the cortical surface in order to recording the constant cortical field potentials and SSEFPs mapped from different areas of somatosensory cortex were analyzed. In the second experiment, SSEPs were recorded in the ipsilateral nucleus gracilis, the contralateral ventroposterolateral thalamic nucleus(VPL), and the cerebral cortex along the conduction pathway of somatosensory information. Results : In the first experiment, we could constantly obtain the SSEFPs in cerebral cortex following the transcutaneous electrical stimulation of the hind limb, and it revealed that the first large positive and following negative waves were largest at the 2mm posterior and 2mm lateral to the bregma in the contralateral somatosensory cortex. The second experiment showed that the SSEPs were conducted by way of posterior column somatosensory pathway and thalamocortical pathway and that specific patterns of the SSEPs were recorded from the nucleus gracilis, VPL, and cerebral cortex. Conclusion : The specially designed recording electrode was found to be very useful in recording the localized SSEFPs and the transcutaneous electrical stimulation using ball electrode was effective in evoking SSEPs. The characteristic shapes, latencies, and conduction velocities of each potentials are expected to be used the fundamental data for the future study of brain functions, including the hydrocephalus model, middle cerebral artery ischemia model, and so forth.

TWINSPAN 및 DCCA 에 의한 한반도 주목림의 군락과 환경의 상관관계 분석

신현철,이강영,송호경 ( Hyun Chul Shin,Kang Young Lee,Ho Kyung Song ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.4

This study was carried out for the purpose of supplying the basic data for artificial forestation, natural regeneration and ecological conservation etc., and obtaining information on alpine vegetation, by establishing vegetation units on the basis of phytosociological classification of community and studying growth pattern on the basis of species composition, hierarchy structure and population dynamics, for Taxes cuspidata naturally growing at the alpine districts in Korea. The importance value of Taxus cuspidata by districts mostly showed above 100 in the upper story but at Mt. Chiri and Mt. Hanra communities its value was comparatively low. In the middle story it showed lower value than that of upper story and not quite showed at Mt. Chiri. Taxus cuspidata communities in the lower story were only in Mt. Hanra, Mt. Odae and Mt. Sobaek, and their importance values were about 10 which were relatively low values. The communities were classified into five groups as Taxus cuspidata-Males baccata var. mandshurica, Taxus cuspidata-Abies nephrolepis, Taxus cuspidata-Abies koreana, Taxus cuspidata-Acer mono and Taxus cuspidata-Euonymus quelpaertensis by TWINSPAN analysis. Taxus cuspidata-Abies koreana community was distributed at the northern aspect of the mountain ridges and at higher elevation than other communities and distributed. Taxus cuspidata-Acer mono community was relatively low than the others and distributed at the hillsides of mountain. And Taxus cuspidata-Euonymus quelpaertensis communities were distributed at the relatively high elevation and northern and eastern aspect of the mountain tap, and Taxus cuspidata-Malus baccata var. mandshurica communities were distributed at the medium elevation, and southern and eastern aspect of the mountain ridge. In the relation between communities and environmental factors, it was correlated with aspect, elevation and topography at the first axis, and elevation, slope at the second axis.

무선 센서네트워크에서 게임이론을 이용한 최적의 클러스터 대역폭 할당

신현철(Shin Hyun Chul),이채우(Lee Chae Woo) 한국통신학회 2009 한국통신학회 학술대회논문집 Vol.2009 No.11

교육 연구자에게 매력적인 분석 대상으로서의 타액

신현철(Shin, Hyun-chul) 학습자중심교과교육학회 2017 학습자중심교과교육연구 Vol.17 No.3

인지적, 정서적 변화는 교감신경과 부교감신경의 활성을 변화시키며 다양한 생리적 변화를 일으킨다. 타액 조성의 변화도 이러한 변화중 하나이다. 본 연구에서는 교육 분야 연구에 대한 적용성이 잘 확인된 타액 단백질, 뉴로펩타이드 및 호르몬을 소개하고 있다. 이러한 타액에 포함된 표지자들의 측정은 교육 연구를 위한 처치의 효과와 학습 과정 동안 일어나는 사고 작용의 상태를 규명하는데 있어서 가치 있는 정보들을 제공한다. 이 리뷰는 선별된 타액 단백질, 뉴로펩타이드, 신경 호르몬들의 특징을 살펴보고, 이 표지자들이 교육 연구에 사용될 때 얻을 수 있는 이점과 전망을 조명하고 있다. Cognitive and emotional changes modulate activity of sympathetic and parasympathetic nerve systems, resulting variety of physiological changes including the composition of salivary substances. A panel of well-evaluated salivary proteins, neuropeptides and hormones are available for the research in the field of education. The measurements of salivary markers provide valuable information to better characterize the effect of pedagogical treatments and psychological status during learning process. This review summarizes the characteristics of selected salivary proteins, neuropeptides and hormones, and discuss the advantages and forcast of using the salivary biomarkers in the educational research.

6-Azauracil 내성을 지닌 Corynebacterium glutamicum 변이주에 의한 L-Lysine의 생산

신현철(Hyun-Chul Shin),김성준(Seong-Jun Kim),전영중(Yeong-Joong Jeon),이재홍(Jae-Heung Lee) 한국생물공학회 1994 KSBB Journal Vol.9 No.4

Bacillus amyloliquefaciens H41이 생산하는 Vibrio anguillarum 생육 저해인자의 정제

신현철(Hyun-Chul Shin),정경태(Kyung-Tae Chung),김광현(Kwang-Hyun Kim),김병우(Byung-Woo Kim),권현주(Hyun-Ju Kwon),이은우(Eun-Woo Lee),염종화(Jong-Hwa Yum),류은주(Eun-Ju Rhu),정유정(Yu-Jeong Jeong),김영희(Young-Hee Kim) 한국생명과학회 2008 생명과학회지 Vol.18 No.6

어류 질병 치료를 위한 probiont의 개발에 목적을 두고 어병균 Vibrio anguillarum NCMB1의 생육에 저해물질을 생산하는 Bacillus amyloliquefaciens H41균주를 분리하고 이 물질의 특성을 규명하기 위하여 정제를 시행하였다. 분리 균을 배양한 배양 상등액을 70% 염석, 투석하여 조 효소액으로 제작하고 조 효소액을 DEAE-sephadex, A-50 ion exchange chromatography, sephadex G-200 gel filtration column chromatography을 통하여 정제하고 SDS-PAGE 를 통하여 단일밴드를 확인하고 최종 회수율 2.9%을 얻을 수 있었으며 40.8배의 정제된 V. anguillarum NCMB1 생육저해물질을 얻을 수 있었다. 정제된 저해물질은 저해정도에 따라 단위를 설정하여 활성을 측정하였으며, 분자량은 48 kDa 로 확인되었으며 정제물질의 활성을 위한 최적 반응 pH와 온도는 pH 7.5와 30℃로 확인되었다. 금속이온의 효과에 있어서는 CoCl₂, HgCl₂, ZnSO₄, AgNO₃에서는 완전히 저해되는 양상을 나타내었고 MgSO₄, MnSO₄에서 미미한 효소활성의 증가를 나타내었다. 그리고 염에 관한 안정성은 일반 해수의 농도인 3%의 농도에서도 활성을 나타내는 것으로 확인되었다. 정제된 저해물질을 현재 상업적으로 사용하고 있는 화학처리제나 항생제와 함께 효율성을 비교 해 보았을 때 저해물질은 약 78%의 저해 활성을 나타내는 것을 알 수 있었고 항생제보다는 효율성이 낮았으나 독성검사를 위해 정제물질을 살아있는 어류에 투여하였으나 어떤 해수어도 폐사하지 않는 것으로 보아 어류 자체엔 독성을 나타내지 않는 물질로 나타났다. 따라서 B. amyloliquefaciens H41 균주가 생산하는 정제 물질이 V. anguillarum 생육에 저해물질로 작용하는 것으로 밝혀졌으며 친환경적인 특성을 가진 물질로 밝혀졌다. To study the possible use of probiotics in fish farming, we evaluated antagonism of antibacterial strain Bacillus amyloliquefaciens H41 against the fish pathogenic bacterium Vibrio anguillarum NCMB1. The purification of growth inhibition factor produced by B. amyloliquefaciens H41 was achieved by obtaining supernatant of this bacterium. The growth inhibition factor was purified to homogeneity by 70% ammonium sulfate precipitation, DEAE-sephadex A-50 ion exchange chromatography, sephadex G-200 gel filtration column chromatography, and sephadex G-50 gel filtration column chromatography with 40.8 fold of purification and 2.9% yield. The molecular weight of the purified growth inhibition factor was 48 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum pH and temperature for the growth inhibition factor were pH 7.5 and 30℃, respectively. The activity of growth inhibition factor was enhanced slightly by some metal ions, such as Mg?², Mn?², but was inhibited by the addition of Co?², Hg?², Zn?² and Ag?². NaCl stability of the growth inhibition factor was observed with 50% residual activity at 3% NaCl concentration. Toxicity test showed that the purified B. amyloliquefaciens H41 growth inhibition factor did not affect the live of Japanese flounder (Paralichthys olivaceus) and the effectiveness was 78% of residual lethality compared to commercial antibacterial agents.

우리나라 고전에 나오는 한자 식물명 ‘삼(杉)’의 분류학적 실체

신현철(Shin, Hyun-chul),기호철(Ki, Ho-chul),홍승직(Hong, Seung- jic) 한국학중앙연구원 2015 한국학 Vol.38 No.3

게임이론을 이용한 클러스터 간 주파수 간섭 문제 연구

신현철(Hyun-Chul Shin),이동열(Dong-Yul Lee),이채우(Chae-Woo Lee) 한국통신학회 2010 韓國通信學會論文誌 Vol.35 No.2

생태학적 관점에 입각한 환경 교육 과정 개발 연구 ( 3 ) - 초 , 중 고등학교 과학 교과서의 환경 관련 내용 분석을 중심으로 -

남현우(Hyun Woo Nam),김태현(Tae Hyun Kim),신현철(Hyun Chul Shin),신현웅(Hyun Woung Shin),조강현(Kang Hyun Cho) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.3

As a comprehensive study on the development of curriculum for environmental education based on the standpoint of ecology, the environmental issues within the science textbooks used in the elementary, middle and high school were analyzed with the special emphasis to four aspects; 1) the causes and 2) the examples of environmental pollution, 3) the principles of ecology, and 4) the endeavor to overcome environmental crises. The results confirm that the science textbooks were written on the basis of Environmentalism rather than Ecology, and the environmental issues were listed fragmentarily within the subjects related to science, and not systematically assembled between inter-subjects or -sciences. Therefore, the systematic and inter-scientific approaches based on the ecology will be needed for further and better environmental education. [environmental education, ecology, textbook analysis].

≪시경(詩經)≫에 나오는 식물명 "저(樗)"의 실체와 한글 번역

신현철 ( Shin Hyun-chur ),기호철 ( Ki Ho-chul ),홍승직 ( Hong Seung-jic ) 중국어문연구회 2016 中國語文論叢 Vol.0 No.75

Chinese letter Chu were twice cited as the plant name in Shijing. Chu had been interpreted as the useless tree or the heaven tree (Ailanthus altissima) recently. To understand Shijing and translate Chu from Chinese into Korean properly, the taxonomic identity of Chinese letter Chu should be verified. From the case study, we have concluded as follows; 1) The Chinese letter Chu had been considered as the useless tree without any botanical characters in Zhuangzi, however from the Post Han, as the heaven tree having the resins in stem, strong smells in leaf, ring porous wood and used as the habitate of spotted lanternfly (Lycorma delicatula) in China. Therefore, Chu in Shijing should be understood as the useless tree, not the heaven tree. 2) The heaven tree is not native plants in Korea, and when this plant was introduced is not known. Chu cited in the Korean literature at Kingdom of Joseon was not the heaven tree. Chu related to King Taejo was confirmed as the birch tree, and included in the post Joseon was the heaven tree. 3) Chu in Shijing should be translated into Korean as ‘bad tree’ or ‘useless tree’, not the heaven tree.