丹參의 methicillin 내성 황색포도구균에 대한 효과

서명원,정승일,신철균,주영승,김홍준,고병섭,Seo, Myung-won,Jeong, Seung-il,Shin, Chol-gyun,Ju, Young-sung,Kim, Hong-jun,Ko, Byoung-seob 대한한방안이비인후피부과학회 2003 한방안이비인후피부과학회지 Vol.16 No.1

Objectives : Gram-positive bacteria have became increasing resistant to antibacterial agents, and hence multi-drug-resistant bacterial pathogens are now a major problem in clinical medicine. There is, therefore, a need for new antibacterial agents. In the course of our screening program for potent antibacterial agent from medicinal plants, the extract of Salvia miltiorrhiza (S. miltiorrhiza) showed antibacterial activity against methcillin resistant Staphylococcus aureus (MRSA) and antibiotic-resistant S. aureus. Methods : S. miltiorrhiza was extracted with 80$\%$ EtOH. The extract was suspended in H2O and fractionated successively with hexane chloroform, ethyl acetate, and n-buthanol. The chloroform fraction, which showed the highest antibacterial activity(MICs, 78㎍/ml) against MRSA, was chromatographed on a silica gel column and recycling prep-LC to give the pure antibacterial component. Results and Conclusions : The second fraction among the chloroform soluble portion of an aqueous EtOH extract of S. miltiorrhiza root showed outstanding antibacterial activity against MRSA and antibiotic-resistant S. aureus compared to the other fraction. An active compound was isolated from the second fraction using silica gel column chromatoraphy and recycling prep-LC. Based on these data together with the IH-, 13C-NMR, mass and mp, the active compounds were identified tanshinone Ⅰ, dehydrotanshinone Ⅰ and cryptotanshinone. Among tanshinones, cryptotanshinone and dihydrotanshinone Ⅰ MICs against MRSA and antibiotics-resistant S. aureus were 12.5, 12.5 and 6.3㎍/ml, respectively.

인진호(茵陳蒿)의 기미론(氣味論)적 해석과 내외부형태연구

이화정,최문일,김자영,강경식,신철균,주영승,윤주봉,Lee, Hwa-Jung,Choi, Mun-Il,Kim, Ja-Young,Kang, Kyoung-Sik,Shin, Chol-Gyun,Jul, Young-Sung,Yun, Ju-Bong 대한한방안이비인후피부과학회 2006 한방안이비인후피부과학회지 Vol.19 No.2

Objectives : The aim of this work is the approaching of properties-flavours theory and the making of morphological standard in Artemisiae herba. Methods : The properties-flavours theory were attempted with bibliographic method and various climate-information. The external-internal morphological standards were determined by using stereoscope and butanol series. Conclusions : The following is a list the conclusion of the approaching of properties-flavours theory and how to make the standard of morphological standard in artemisiae herba. 1. The significant analysis for the artemisiae herba as the properties-flavours theory, is a follow-up survey of the effect written in the official oriental medicine book 2. The other analytic methods according to the gathering time, processing or storing system or especially nature or growth environment(altitude, temperature,. the mean moisture, and the agronomical survey) by way of the explanation of properties-flavours theory appears so many insufficiency at the many cases. 3. In the case of artemisiae herba., there are 2 cases(Artemisia capillaris $T_{HUNB}$ and A. iwayomogi $K_{ITAMURA}$) in current and recently A. aruwa L. is substituted for artemisiae herba in circulation. 4. The external and internal characteristics according to the shape of original plant or herbal states, entirely correspond to all the official oriental medicine boo 5. According to the place of productiot the difference appeals. (1) A. capillatis $T_{HUNB}$. has leaves like a fine thread, and A. iwayomogi $K_{ITAMURA}$ has big and wide leaves, and shows some reddish color, and A. aruwa L. has narrow leaves, its section lines long vertically. (2) In the internal shapes, as the A. capillatis $T_{HUNB}$. leaves', upper epidermal cell wall shows relatively straight form, its all upper- lower epidemical cell wall has a stoma, and calcium rosette crystal is relatively few. As iwayomogi $K_{ITAMURA}$, all upper- lower epidermal cell wall is wavelike concave, and hypodermics has many stoma and many calcium rosette crystal. (3) In the internal shapes of A. aruwa L. the shape of leaves' epidermal cell is irregular and has so many non-glandular hair nad glandular hair on its surface.

길경(桔梗)의 내외부형태 연구

이화정,최문일,윤주봉,김자영,강경식,신철균,주영승,Lee, Hwa-Jung,Choi, Mun-Il,Yun, Ju-Bong,Kim, Ja-Young,Kang, Kyoung-Sik,Shin, Chol-Gyun,Ju, Young-Sung 대한한의학방제학회 2006 大韓韓醫學方劑學會誌 Vol.14 No.1

Objectives: The aim of this work is the making of morphological standard in platycodi radix. Methods: The external-internal morphological standards were determined by using stereoscope and butanol series. Conclusions: The external and internal characteristics according to the shape of original plant or herbal states. entirely correspond to all the literature on this subject. According to the place of production. the difference appears. (1) In the external shapes. as the Korean products. the distinction among the first. medium. poor class products was caused by the state of processing of desiccation. But as the foreign products, it was caused by the difference of size, color and especially color of circle. (2) In the internal shapes. the Mt. Baekdu region products is not skinned and has the deep state of suberization of cork phellogen. so it makes certain distinctional point. And the Korean products has the much latex tube group relatively and the bigger crevice than the foreign one.

야국의 형태 및 자유라디칼 소거 물질에 관한 연구

서명원 ( Myung Won Seo ),정승일 ( Seung Il Jeong ),신철균 ( Chol Gyun Shin ),주영승 ( Young Sung Ju ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.1

N/A Objectives : The purpose of the study is to enhance the objectivity and availability of the oriental herb medicine by formulating the morphological standard and isolation and structure elucidation of radical scavengers from Chrysanthemum indicum L. These intents are expected to be useful in the examination of several physiochemical and biological reactions, determination of indicator material, and identification of gene with basic sources in the future. Methods : In order to develop the standard formulas and isolation and structure elucidation of radical scavengers from Chrysanthemum indicum L., the experimental examinations of the original herbs have been carried out. The morphological standard was divided into 4 parts as plant · external · internal · powder shape. Three antioxidative compouds were isolated by a bioassay using a 1, 1-diphenyl-2-picrydrazyl(DPPH) free radical. They were identified as luteolin, apigenin and linarin (acacetin-7-O-rutinoside) on the basis of 1H and 13C-NMR and MS data. Results and Conclusions : 1. The plant shape : The color of the slender-hair in its stem is purple-black. The leaf is oval-orbicular shape and has the slender-hair in both sides and its petiole has pseudo-stipule. The entire diameter of flower is 2~2.5cm. The lingulate flower is yellow and the siphonoic flower is deep-yellow color. 2. The external shape of herb: It is a globular-like type, 2~3cm in diameter and brown-yellow to yellow color. The lingulate flower is yellow-white color haved one layer and the siphonoic flower is deep-yellow color. The external layer is oval-velvet and the internal layer is long-oval shape in the bracteole. 3. The internal shape of herb: The change of the petal is relatively large, the upper epidermal cell is irregular oval or sphere shape and the lower epidermal cell is regular sphere shape. The lower epidermal cell has occasionally trichomes or a few air pores. the mesophyll tissue has small thin-walled cells which are relatively dense, and the secretory tissue and reserve substances of the pipe type. The vascular bundle which has reticulate vessel is distributed near to lower epidermis cells. 4. The powder shape of herb: The color of the powder sample is yellow-brown. The fragments of parenchyma, vessel and the calcium oxalate crystal are observed. In the sample of maceration, the epidermal cell of petal is various, the epidermal cell of filament is many side shape. The parenchyma is mostly oval shape which occasionally concludes the calcium oxalate crystal. The vessel has uniforation plate at each edge, which is a spiral vessel, and the type of the pit of lateral wall is alternate shape 5. Isolation and Structure Elucidation of Radical Scavengers: The DPPH radical scavenging activity (RC_50: 13.3㎍) of apigenin was similar to that of α-tocopherol (12.1㎍) and L-ascobic acid (9.8㎍).

산수유(山茱萸) 기미론(氣味論)적 해석과 내외부형태연구

최문일 ( Mun Il Choi ),이화정 ( Hwa Jung Lee ),윤주봉 ( Ju Bong Yun ),김자영 ( Ja Young Kim ),강경식 ( Kyoung Sik Kang ),신철균 ( Chol Gyun Shin ),주영승 ( Young Sung Ju ) 대한본초학회 2006 大韓本草學會誌 Vol.21 No.2

赤何首鳥와 白何首鳥의 外部 및 內部形態

宋坰松,申喆均,朱榮丞 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objectives : This study was designed to establish a characteristic discrimination of internal and external morphological standard of original plants and herbal states in Polygoni Multiflori and Cynanchi Wilfordii Radix. Methods : In this studies, the external-internal morphological standards were determined by using stereoscope and butanol series. Results : 1. The external characteristics of original plants: Polygonum multiflorum has alternate leaves, and its flower with white color blooms at the shoot apex or leaf axil. In the other hand, Cymchum wilfor&i has opposite leaves, and its flower with yellowish green color blooms at the leaf axil. 2. The physical characteristics of herbal states: Polygoni Multiflori Radix is red-brown in outer surface, pink-brown in section. In the other hand, Cynanchi Wilfordii Radix is earthly-brown in outer surface, greyish white in section. 3. The physical characteristics according to the place of production: Demonstrable difference according to the place of production is not seen. Polygoni Multiflori Radix is brown or dark brown in outer surface, soft yellow-dark brown in section. In the other hand, Cynanchi Wilfordii Radix is white in outer surface, white-weak red in section, and has power type. 4. The internal characteristics: Pofygonum mulQt7orum has hetero-vascular bundle and lignification of cork layer is progressive. In the other hand, cynanchum wilfordii has not hetero-vascular bundle, and lignification of cork layer is weak. Conclusion : In the future, many fundamental study such as how to discriminate between Polygoni Multiflori Radix and Cynanchi Wilfordii Radix in origin and efficacy will be necessary. Also the standard of discrimination must be specific and distinct in that several kinds of fo-ti has been traded.