한의학의 발전 방향 검토

신길조,Shin, Gil Cho 대한중풍순환신경학회 2017 대한중풍.순환신경학회지 Vol.18 No.1

It is difficult to combine oriental and western medicine. Each medicine has a different academic background, perspective on the world, and studying methodology. The claim that two paradigms can not be combined is not obtained from an actual verification, but this means it is so hard to combine two medicines having different backgrounds. The amalgamation of oriental and western medicine should be phased in on the basis of the continuous reciprocal understanding and commitment. First, the strength and weakness of each medicine over the treatment and research are required to be identified. Then, a few complementary areas can be chosen enabling a trial of fusion on a small scale. A cycle of problem solving and a new research can be set by analyzing research results obtained through the implementation over a period of time. In other words, the researchers of oriental and western medicine should repeat a continuous and gradual complementary research process by identifying issues to be improved and complemented through a consensus. Once the methodology obtained through the process of problem solving and proficient implementation is established in a stable condition, a method to widen the fusion area by expanding the operating area and implementation method can be chosen. However, the integral system of oriental medicine shouldn't be substituted by mechanical idea or reductionism. What should be done primarily for oriental medicine is to objectify things through quantification. In particular, the oriental treatment should accept the microscopic diagnosis to determine the structure and observe the biochemical change.

中風患者의 左·右半身不遂에 관한 臨床的 觀察

辛吉祚(Shin Gil-Cho),曺基湖(Cho ki-ho),金永錫(Kim Young-Suk),裵亨燮(Bai Hyoung-Sup),李京燮(Lee Kyung-Sup) 대한한의학회 1991 대한한의학회지 Vol.12 No.1

흰쥐의 중대뇌동맥 결찰로 유발된 뇌허혈에서 양격산화탕(凉膈散火湯)이 신경세포에 미치는 효과

신길조,오경환,정승현,이원철,Shin, Gil-cho,Oh, Kyung-hwan,Jeong, Sung-hyun,Lee, Won-chul 대한중풍순환신경학회 2006 대한중풍.순환신경학회지 Vol.7 No.1

Objectives : Yanggyuksanhwa-tang is a prescription used for cerebral infarction clinically. Methods : According to previous research data, the effect of Yanggyuksanhwa-tang on cerebral infarction, we induced cerebral infarction by middle cerebral artery occlusion(MCAO) in rats, and the rats were administered Yanggyuksanhwa-tang. Results: Infarct area, infarct volume were measured, and the level of elements such as c-Fos, Bax and caspase-3 in penumbra of infarct were expressed by immunohistochemical staining. Conclusion : Yanggyuksanhwa-tang showed neuroprotective effect through preventing neuronal cell apoptosis.

고혈압(高血壓) 및 고지혈증(高脂血症)에 대(對)한 청열도담탕(淸熱導痰湯)의 실험적(實驗的) 연구(硏究)

이대식,신길조,조기호,김영석,배형섭,이경섭,Lee, Dai-Sik,Shin, Gil-Cho,Cho, Ki-Ho,Kim, Young-Suk,Bai, Hyung-Sup,Lee, Kyung-Sup 대한한방내과학회 1991 大韓韓方內科學會誌 Vol.12 No.2

Mahwangsan is used in the treatment of asthma due to wind and cold(風寒喘). This is study was carried out to investigate the effect of Mahwangsan extract and In order to investigate the effects of Chungyeuldodamtang experimental studies were performed in hyperlipidemia Rats induced by cholesterol and Spontaneusly hypertension rats. The contents of serum total cholesterol, triglyceride, phospholipid and FFA and the changes of blood pressure and heart beat were measured. The results were summarized as follows ; 1. The content of Total-Cholesterol in the serum compared with control group tended to be decreased in Chungyeuldodamtang group, but did not show a significance. 2. The content of Triglyceride in the serum was significantly decreased in Chungyeuldodamtang group. 3. The content of Phospholipid in the serum was significantly decreased in Chungyeuldodamtang group. 4. The content of FFA in the serum tended to decreased in Chungyeuldodamtang group, but did not show a significance. 5. The S.H.R's blood pressure was significantly decreased in Chungyeuldodamtang group. 6. The S.H.R.'s Heart beat compared with control group tended to be decreased in Chungyeuldodamtang group, but did not show a significance. According to the above results, it is assumed that Chungyeuldodamtang has a valid effect on Hypertension and Hyperlipidemia.

우황청심원(牛黃淸心元)이 저산소증 유발 배양신경세포에 미치는 영향

조규선,신길조,문일수,이원철,정승현,Cho, Gyu-Seon,Shin, Gil-Cho,Moon, Il-Soo,Lee, Won-Chul,Chung, Sung-Hyun 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.2

Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast. Methods : To evaluate the effect of Woohwangcheongsim-won on neuronal death caused by hypoxia, the survival rate of E18 cortical neuroblast was measured with MTT assay and the changes of several synaptic proteins and enzymes were investigated with the immunoblot assays. Results : The E18 cortical neuroblasts were added 50, 100, 500, 1,000, and $5,000{\mu}g/ml$ Woohwangcheongsim-won. They showed neurotoxicity, when the concentration of Woohwangcheongsim-won was above $1,000{\mu}g/ml$. The E18 cortical neuroblasts, which were added 50, 100, and $500{\mu}g/ml$ Woohwangcheongsim-won, were exposed 98% $N_2/5%\;CO_2$ for 3 hours to induce hypoxia, 3 days later, the survival rate of $50{\mu}g/ml$ Woohwangcheongsim-won was 141.5% when compared to the control group. On the immuneblot assays, the expressions of ${\alpha}$CaMKII, NR2A, NR28, PDE2, PSD-95, and eEF-$1{\alpha}$ were increased in normoxia, but those of NR2A, NR2B were decreased in hypoxia when compared to the control group. Conclusions : The data shows that the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast is a significant result.

배양한 흰쥐 대뇌세포의 저산소증 모델에서 황금(黃芩)이 유전자 표현에 미치는 영향

정승현,신길조,이원철,김성배,Chung, Sung-Hyun,Shin, Gil-Cho,Lee, Won-Chul,Kim, Sung-Bae 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.4

Objectives : The purpose of this investigation is to evaluate the effects of Scutellaria baicalensis GEORGI on alteration in gene expression in a hypoxia model using cultured rat cortical cells. Methods : E18 rat cortical cells were grown in a Neurobasal medium containing B27 supplement. On 12 DIV, Scutellaria baicalensis GEORGI(20 ug/ml) was added to the culture media and left for 24 hrs. On 11 DIV, cells were given a hypoxic insult $(2%\;O_2/5%\;CO_2,\;37^{\circ}C,\;3\;hrs)$, returned to normoxia and cultured for another 24 hrs. Total RNA was prepared from Scutellaria baicalensis GEORGI-untreated (control) and -treated cultures and alteration in gene expression was analysed by microarray using rat 5K-TwinChips. Results : For most of the genes altered in expression, the Global M values were between -0.5 to +0.5. Among these, 1143 genes increased in their expression by more than Global M +0.1, while 1161 genes decreased by more than Global M -0.1. Effects on some of the genes whose functions are implicated in neural viability are as follows: 1) The expression of apoptosis-related genes such as Bad (Global M = 0.39), programmed cell death-2(Pdcd2) (Global M = 0.20) increased, while Purinergic receptor P2X(P2rxl) Global M = -0.22), Bc12-like1(Bc1211)(Global M = -0.19) decreased. 2) The expression of 'response to stress-related genes such as antioxidation-related AMP-activated protein kinase subunit gamma 1 gene (Prkag1) (Global M = 0.14), catalase gene (Global M = 0.14) and Heme Oxygenase(Hmoxl) increased. 3) The expression of Fos like antigen 2 (Fos12) expressed in neurons that survive ischemic insult increased (Global M = 0.97). Conclusions : these data suggest that Scutellaria baicalensis GEORGI increases the expression of antiapoptosis- and antioxidation- related genes in a way that can not yet be explained.

심양허증으로 변증한 확장성 심근병증 환자 치료 1례

하유빈,신길조,Ha, Yu-bin,Shin, Gil-cho 대한중풍순환신경학회 2019 대한중풍.순환신경학회지 Vol.20 No.1

■ Objectives The purpose of this study is to report the improvement of dilated cardiomyopathy after traditional Korean medical treatment; herb-med, acupuncture, moxibustion. ■ Methods A patient with cardiomyopathy whose chef complaint was oversleep, shortness of breath, fatigue and coldness of hand-foot received inpatient treatment from September 2^nd 2019 to September 23^rd 2019. And we analyzed treatment progress and result. ■ Results We observed that traditional Korean medical treatment decreased symptoms; Fatigue by 30% and Coldness of hand-foot by 100%. And The patient's general condition is improved. ■ Conclusion We report this clinical study to be helpful in treating patients of dilated cardiomyopathy by traditional Korean medicine.

청상사화탕(淸上瀉火湯)을 투여하여 두통(頭痛)이 호전된 경막하혈종 환자 2례

김솔리,신길조,김승현,Kim, Sol-ri,Shin, Gil-cho,Kim, Seung-hyeon 대한중풍순환신경학회 2010 대한중풍.순환신경학회지 Vol.11 No.1

Objectives : This cliniclal study was to evaluate the effect of Cheongsangsahwatang (淸上瀉火湯) treatment on the headache after subdural hemorrhage. Methods : We treated two patients who have headache after subdural hemorrhage by oriental medical therapy, specially Cheongsangsahwatang (淸上瀉火湯). We used VAS(Visual Analog Scale) to investigate the effect of oriental medical therapy, specially Cheongsangsahwatang (淸上瀉火湯). Reasults : After prescription of Cheongsangsahwatang (淸上瀉火湯), VAS scores on headache and accompanying symptoms decreased. Conclusions : This study suggests that Cheongsangsahwatang (淸上瀉火湯) is effective in the treatment of headache after subdural hemorrhage.

오미자(五味子)(Fructus Schisandrae)가 흰쥐 해마신경세포의 저산소증 모델에서 세포사에 미치는 영향

주대환,신길조,문일수,Ju, Dae-Hwan,Shin, Gil-Cho,Moon, Il-Soo 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.4

Objectives : The purpose of this study was to investigate the effect of FS for the modulation of ROS and MMP in a hypoxic model of cultured rat cortical cells. Methods : For the effect of FS on the viability, FS was added to culture media (neurobasal supplemented with B27) and cell viability was measured by LDH assay. To investigate the effects of FS on ROS generation and MMP preservation, cells grown in FS-containing media were given a hypoxic shock(2% $O_2/5%$ $CO_2$, $37^{\circ}C$, 3 hrs) on DIV 10, stained with $H_2DCF-DA$(10 nM) and JC-1, respectively, and observed by fluorescent microscope. Results : 1. FS has a protective effect of cortical cells in both normoxia and hypoxia. 2. FS reduced the generation of ROS and this reduction was especially significant at 3 days after hypoxia. 3. FS was effective for the maintenance of MMP in hypoxia, and this efficacy was especially significant at 3 days after hypoxia. Conclusions : Taken together, these results indicate that FS attenuates ROS generation and MMP dissipation, which eventually protects from neuronal cell death in hypoxia.

목향(木香) 및 사향(麝香)이 저산소증 유발 배양 대뇌신경세포에 미치는 영향

정승현,신길조,이원철,문일수,류도균,Jeong Sung-Hyun,Shin Gil-Cho,Lee Won-Chu,Moon Il-Su,Ryu Do-Kyun 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.2

Objectives : The purpose of this investigation is to evaluate the effects of Aucklandiae Radix Moschus(木香 麝香)and to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20 (E20) cortical cells of a rat (Sprague Dawley). Methods : E20 cortical cells used in this investigation were dissociated in Neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Aucklandiae Radix Moschus(木香 麝香) was added to the culture media for 72 hrs. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Moschus(麝香)'s effects for neuronal death protection were evaluated by LDH assay and the mechanisms were studied by Bcl-2, Bak, Bax, caspase family. Results : This study indicate that Aucklandiae Radix(木香)'s effects for neuronal death protection in normoxia and Scutellariae Radix(麝香)'s effects for neuronal death protection in hypoxia were confirmed by LDH assay in culture method of Embryonic day 20(E20) cortical neuroblast. Moschus(麝香)'s mechanism for neuronal death protection in hypoxia is to increase the anti-apoptosis protein Bcl-2. Conclusions : It may be reasonable to propose that Moschus(麝香) protects delayed neuronal death in hypoxia by increasing Bcl-2, thereby reducing mitochondrial permeability transition(PT) pores, the cytochrome c channels.