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생쥐 발달과정 중 난포 내 c-Kit/SCF 및 Inhibin-α 단백질의 발현 변화
강재성 ( Kang Jae Seong ),이창주 ( Lee Chang Ju ),라중열 ( Rha Jung Yeol ),이종민 ( Lee Jong Min ),송강원 ( Song Gang Won ),박문향 ( Park Mun Hyang ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.7
목적 : 포유류 난포의 성장에 관여하는 기전은 밝혀져 있지 않으나, 줄기세포인자 (stem cell factor, SCF)는 난소의 성장과 발달에 관여하며, inhibin-α는 과립막세포의 분화에 관여하는 인자로 알려져 있다. 본 연구는 발달 단계에 따라 생쥐 난포 내 c-kit, 줄기세포인자 (stem cell factor, SCF)와 inhibin-α의 발현 변화를 면역조직 화학적 방법을 통해 조사하여 c-kit, SCF, inhibin-α 단백질이 난포성장의 어느 시기에 발현하는지를 알아보기 위하여 시행하였다. 연구 방법 : 임신 14일, 16일, 생후 2일, 7일, 그리고 21일된 생쥐 난소를 채취하여 4% paraformaldehyde에 고정한 후 c-kit/SCF, inhibin-α의 면역조직화학 염색을 실시하였다. 결과 : 태자의 경우 난모세포에서 c-kit/SCF의 발현을 확인하였으며, 신생자의 경우에는 원시난포 및 일차난포의 난자에서 SCF가 발현되었다. 미성숙 생쥐의 경우 난포의 발달에 따라 난자 내 c-kit/SCF의 발현이 감소 되었으며, SCF는 전동난포의 난자에서 강하게 발현되었다. Inhibin-α는 미성숙 생쥐의 전동난포 및 초기 동난포의 과립막세포에서 발현되었다. 결론 : c-kit/SCF의 발현은 난포의 성장이 개시된 이후에 감소되며, inhibin-α 의 발현이 증가한 난포에서 c-kit 및 SCF 단백질의 발현이 감소한다는 것을 알 수 있다. 따라서 c-kit/SCF 단백질은 난포가 성장을 개시하기 이전에 작용하는 것으로 생각한다. Objective : It is known that SCF stimulates cellular migration, prolifertation, differentiation and survival upon interaction with its receptor, c-kit. SCF is essential for a normal gonadal development. Inhibin-α subunit gene expression is important for granulosa cell (GC) differentiation. The mechanism concerning the development of the ovarian follicles is not clearly understood. In this study, an immunohistochemical study was performed to find out the follicular expressions of c-kit, SCF, and inhibin-α during different developmental stages in mouse ovary. Materials & Methods : Mouse ovaries obtained from 14 and 16 days post coitum and 2, 7, and 21 days post partum were fixed in paraformaldehyde. Immunohistochemistries for c-kit, SCF, inhibin-α were subsequently carried out. Results : Antigens for both c-kit and SCF were expressed constantly on oogonia regardless of their developmental stages. SCF was expressed on oocytes of primordial and primary follicles of neonate mouse ovaries. In immature mouse ovaries, there was a gradual decrease in expression of c-kit/SCF as the follicles develop. SCF was expressed strongly on the oocytes of preantral follicles, but weakly in granulosa and theca cells. Inhibin-α showed a large expression on granulosa cells of preantral and early entral follicles of the immature mouse ovaries. Conclusion : We concluded that the expression of c-kit/SCF is decreased after the follicle start to grow. The expression of inhibin-α is negatively correlated to the expressions of both c-kit/SCF in the ovarian follicles in mice.
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양용석(Yong-Suk Ryang),류장근(Jang-Keun Ryu),주난영(Nan-Young Ju),송강원(Kang-Won Song) 대한의생명과학회 1996 Biomedical Science Letters Vol.2 No.2
저자들은 마우스를 실험모델로 하여 간흡충의 항원을 투여 했을 때 비장조직에 대한 CD3, CD4 및 CD8 모노클로날 항체의 반응 여부를 알아보고자 하였다. 즉, 간흡충에 대한 세포면역학적인 특성을 규명고자 하였으며 특히 비장 조직에 대한 phenotype을 관찰한 결과 다음과 같은 결과를 얻었다. 간흡충의 조항원을 면역증강제와 함께 복강 투여한 다음 일정 기간 후에 비장조직을 Avidin-biotin complex 면역조직염색을 실시한 결과 CD3에서 강한 양성 반응을 나타냈고 CD4와 CD8에서는 약한 반응을 나타냈다. 조직부위를 보면 피막, 혈관, 임파관, 백수부위와 림프구 및 대식세포의 세포막에서 양성반응을 보였다. The authors inquired into what reactions comprise the response of mice(as a model) CD3, CD4 and CD8 monoclonal antibodies in spleen tissue when injected intraperitoneally by antigens of Clonorchis sinensis. The author is objective was focused on investigating the property of cellular immunity for liver fluke. In particular, the results of having examined the phenotype of the tissue of spleen were revealed as follows: a certain length of time after having been intraperitoneally injected with antigens of Clonorchis sinensis and Freund's adjuvant, the tissue of spleen was embedded and immunohistochemically stained by the avidin-biotin complex method. A strong reaction in response to CD3, while a feeble reaction resulted from CD4 and CD8. The tissue region showed a positive reaction to all antibodies, especially from capsules, vascular areas, white pulps and membrane of blood cells.
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류마티스관절염 환자의 활막세포에서 Stat 단백의 발현에 관한 연구
심승철 ( Seung Cheol Shim ),이인홍 ( In Hong Lee ),정자헌 ( Ja Hun Jung ),김태환 ( Tae Hwan Kim ),전재범 ( Jae Bum Jun ),송강원 ( Kang Won Song ),배상철 ( Sang Cheol Bae ),유대현 ( Dae Hyun Yoo ),김성윤 ( Seong Yoon Kim ) 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.1
Objective: To characterize Stat activity in synovial tissue in rheumatoid arthritis (RA) in order to see if Stat molecule contributes to the pathogenesis of RA by regulatory expression of genes that play an important role in inflammation and tissue destruction. Methods: Synovial tissue were obtained immediately after operative excision. Immuno-histochemistry was done with the antibodies for Stat 3 and Stat 5. Cells were stimulated with interleukin 6 (IL-6) and soluble interleukin 6 receptor (sIL-6R) or steroid using chambered slide. In supershift experiment, cell extracts were incubated with 0.5ng of 32P-labelled double-stranded oligonucleotide probe. Samples were resolved on 4.5% polyacrylamide gels, which was transferred to polyvinylidene fluoride membranes. Anti-phosphotyrosine Stat 3 antibody was used for Western blotting. Results: Stat 3 was not shown on the synovial tissue section done by immuno-histochemistry. However, activated Stat 3 was expressed on cultured synovial cell stimulated with IL-6 and sIL-6R, and also with IL-6 and dexamethasone using chambered slide. In contrast to Stat 3, activated Stat 5 was expressed on the synovial tissue section, especially around blood vessel. Conclusion: Stat is activated in cultured synovial cells as shown in other immune associated cells, and IL-6 is the strong activator of Stat 3. Further analysis of the regulation of Stats in synovitis and the role of Stats in driving synovial inflammation will yield insight into the pathogenesis of RA and the development of novel therapeutic modality.
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