폐에 발생한 원발성 인환세포암종 - 2예 보고 -

김동자,이숙희,손윤경,Kim, Dong-Ja,Lee, Sook-Hee,Sohn, Yoon-Kyung 대한세포병리학회 1997 대한세포병리학회지 Vol.8 No.1

Signet ring cell carcinoma is a variant of adenocarcinoma and has been rarely reported in the lung as a primary site. Recently, we experienced two cases of primary signet ring cell carcinoma in the lung without any other extrapulmonary lesion. Sputum cytology was performed and the tumor cells which have eccentrically located nuclei and abundnat mucinous cytoplasm were dispersed in diffuse sheets. On resected specimen, the signet ring cells occupied about $50{\sim}80%$ of all tumor cell nests. Histochemical staining revealed that the mucin produced by tumor cells was mostly carboxylated acid mucins. Ultrastructurally, the tumor cells contained variable sized membrane-bound mucin granules with weak central osmilophilic density and showed numerous surface microvilli, which represented that tumor cells arose from bronchial epithelial cells. In general, this tumor has diffusely infiltrative nature and the prognosis is fatal due to widespread metastasis before clinical discovery.

두경부 편평상피세포암의 p53단백과 PCNA 및 Ki-67의 발현양상

이은진,이상한,손윤경,Lee, Eun-Jin,Lee, Sang-Han,Sohn, Yoon-Kyung 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.2

To investigate the correlation between the clinical features and the expression of p53, PCNA, and Ki-67 of the head neck squamous cell carcinoma, immunohistochemicalstaining of p53, PCNA, and Ki-67 on the paraffin embedded tissue blocks of 116 surgically removed specimens were done. The staining intensity was divided as grade 1 to grade 3 and the results were statistically analysed. 1. The positive reation rates of cell proliferation markers (PCNA and Ki-67) were higher than that of p53. There was significant correlations of the PCNA and Ki-67 expression but there was no significant correlations between p53 and PCNA or p53 and Ki-67. 2. There were no significant correlation between the expression of p53, PCNA and Ki-67 and tumor site or tumor size. 3. There was no significant differences in the positive response according to the nodal status. The node metastasis groups revealed that higher proportion of grade 3 staining of PCNA and Ki-67 than node negative group. From the above results it is concluded that p53 and cell proliferation markers PCNA and Ki-67 might have their unique mechanism involving in the growing and progression of tumor. Overexpression of p53 does not appear to represent an independent prognostic marker in head neck squamous cell carcinoma.

고관절 치환술후 무균성 해리와 골용해 조직에서 세포사멸

김신윤(Shin-Yoon Kim),인주철(Joo Chul Ihn),손윤경(Yoon Kyung Sohn),김정완(Jung Wan Kim),신홍인(Hong Ihn Shin),구경회(Kyung Hoi Koo) 대한정형외과학회 2003 대한정형외과학회지 Vol.38 No.3

신생쥐의 저산소성 허혈성 뇌손상에서 손상 후 덱사메타손의 투여 효과

박창로,박경필,김행미,손윤경,Park, Chang Ro,Park, Kyung Pil,Kim, Heng Mi,Sohn, Yoon Kyung 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.10

목 적 : 미숙아들은 심혈관계 및 호흡기계의 미숙으로 만삭아 보다 주산기 저산소증의 발생 가능성이 높으며 만성 폐질환의 예방과 치료를 위한 출생 후 코르티코스테로이드 제제의 투여 기회 또한 높다. 저산소-허혈 즉 주산기 가사가 일어나는 경우 비가역적 손상을 나타내는 기관은 뇌가 유일하므로 뇌손상 정도는 장기적 예후와 직결된다. 이에 저자들은 저산소-허혈에 의한 뇌손상에서 코르티코스테로이드 제제 중 신생아에게 가장 빈번하게 쓰이고 있는 덱사메타손이 신생아 뇌손상에 어떠한 영향을 미치는지를 조사하기 위해 이 연구를 시행하였다. 방 법: 본 연구에는 10마리의 Sprague-Dawley rat 어미에서 태어난 103마리의 신생쥐를 총경동맥 절단과 산소 농도 8%에 노출시켜 저산소-허혈 모형을 만들고 덱사메타손체중 kg 당 0.5 mg을 투여하였다. 적출뇌는 terminal-deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate nick end labelling(TUNEL) 염색, Bcl-2 및 Bax 형광염색, TUNEL 및 synaptophysin 이중 염색을 시행하여 세포 자멸사 양상을 관찰하고 경색부위 면적은 백분율로 구하여 대조군과 비교하였다. 결 과 : 허혈-저산소 환경 노출 후 생존한 95마리 중 덱사메타손 투여군은 54마리, 대조군은 41마리였다. 양육 중 덱사메타손 투여군 가운데 25마리가 사망하였고 대조군은 7마리가 사망하여 덱사메타손 투여군에서의 사망률이 의의 있게 높았다(46.3% vs. 17.1%, P=0.002). 생후 7일째 저산소-허혈 및 투약 전 체중은 두 군에서 차이를 보이지 않았으나 생후 14일째 측정한 체중은 덱사메타손 투여군의 체중 증가가 유의하게 적었다(P=0.001). Bax 및 Bcl-2, Bax/Bcl-2 및 TUNEL 염색 소견은 측정 단위당 Bax가 덱사메타손 투여시 유의하게 증가되었고 Bax/Bcl-2 및 TUNEL 양성세포 역시 덱사메타손군에서 다소 높은 것으로 나타났으나 통계적 유의성은 없었다. 덱사메타손군의 뇌손상 부위는 대조군과 의미 있는 차이를 보이지 않았다. 결 론: 저산소-허혈 후 덱사메타손 투여는 체중 증가 방해, 사망률 증가 등 전신 상태의 악화를 초래하며 뇌세포 자멸사를 조장할 가능성이 있다. Purpose : Dexamethasone is frequently administered to prevent or treat chronic lung disease in human neonates who are also prone to hypoxic-ischemic(HI) insults. Recently, meta-analysis of the follow-up studies reveals a significantly increased odd ratio for the occurrence of cerebral palsy or an abnormal neurologic outcome, and there is conflicting evidence regarding the impact of dexamethasone exposure on HI brain injury. This study was conducted to explore the effect of post-HI dexamethasone administration on neuronal injury in neonatal rats. Methods : HI was produced in seven-day-old rats by right carotid artery ligation followed by two hours of 8% oxygen exposure. At the end of HI, the animals were injected intraperitoneally either with dexamethasone(0.5 mg/kg) or saline. Neuronal injury was assessed seven days after the HI by the area of infarction, TUNEL reactivity, Bcl-2 and Bax expression in brain. Results : Post-insult dexamethasone administration resulted in reduction of weight gain and a higher mortality rate during seven days after HI. Dexamethasone treatment revealed no effect on the size of brain infarction induced by HI. Bax protein expression increased in dexamethasone treated brain but Bcl-2 protein expression and TUNEL reactivity revealed no significant differences between dexamethasone treated and non treated brain. Increased Bax protein expression suggest upregulation of the apoptosis by dexamethasone. Conclusion : The result suggests the adverse role of Post-HI administration of dexamethasone in neonatal HI.

갑상선 종괴로 촉지된 흉선종의 세침흡인 세포학적 진단 - 2예 보고 -

김동자,박지영,금윤섭,박태인,손윤경,Kim, Dong-Ja,Park, Ji-Young,Kum, Yoon-Seup,Park, Tae-In,Sohn, Yoon-Kyung 대한세포병리학회 2000 대한세포병리학회지 Vol.11 No.1

Thymoma is the most common anterior mediastinal tumor in adults. Rarely, it is presented as the anterior neck mass, commonly located in the anterolateral aspect of the neck or adjacent to the thyroid. We experienced two cases of fine needle aspiration cytology of thymoma, mimicking thyroid mass. The first case was an ectopoic cervical thymoma in a 31-year-old female. The fine needle aspiration cytology was misinterpreted as reactive hyperplasia of lymph node. But the histologic diagnosis was thymoma, predominantly lymphocytic type. The second case was an invasive thymoma in a 66-year-old female, who complained a large anterior neck mass. The fine needle aspiration cytology revealed biphasic population of some clusters of epithelial cells and scattered lymphocytes. The cytologic diagnosis was thymoma and was confirmed as invasive thymoma after the biopsy. Therefore, when the cytologic feature of anterior neck mass shows the both lymphocyte and epithelial component, the differential diagnosis should Include the possibility of thymoma.

Glutathione S-transferases (GSTM1, GSTT1 and GSTP1) and N-acetyltransferase 2 Polymorphisms and the Risk of Gastric Cancer

홍수형,김정완,김호각,박인규,류준욱,이창형,손윤경,이종영,Hong, Su-Hyung,Kim, Jung-Wan,Kim, Ho-Gak,Park, In-Kyu,Ryoo, Jun-Wook,Lee, Chang-Hyeong,Sohn, Yoon-Kyung,Lee, Jong-Young The Korean Society for Preventive Medicine 2006 예방의학회지 Vol.39 No.2

Objectives : Polymorphisms of genes from glutathione Stransferases (GSTs) and N-acetyltransferase 2 (NAT2) have been associated with increased susceptibility to various cancers. Previous results showed that East Asians such as Koreans, Japanese and Chinese have a much higher frequency of the GSTM1 and GSTT1 null genotypes and NAT2 rapid acetylator type. Therefore, we investigated the association between the polymorphic types of GSTs (GSTM1, GSTT1, GSTP1) and NAT2 and the incidence of gastric cancer which is one of the most prevalent cancers among the East Asians. Methods : It was performed in a case-control study consisting of 238 healthy subjects and 108 cancer patients (54 distal and 54 proximal carcinomas). We also evaluated the association between GSTs and NAT2 and the risk factors for gastric cancer such as alcohol consumption, smoking, H. pylori infection, family history of gastric cancer, and tumor location. Results : In our study, the percentage of cases whose hometown was rural was higher than those of controls (odds ratio (OR) =2.88; 95% CI=1.72-4.76), and the frequency of the lower socio-economic status increased significantly in patients (OR=2.53; 95% CI=1.59-4.02). There was no significant difference in the GST polymorphic types between the cases and controls. However, NAT2 rapid or intermediate acetylator types were frequently detected in the cases with family history of gastric cancer (OR=1.92; 95% CI=1.79-26.0). Conclusions : These results suggest that the hometown and socio-economic status are important environmental factors for gastric carcinogenesis, and NAT2 polymorphic types could be associated with familial gastric carcinoma.

만성 (慢性) 활동성 (活動性) 간염에서 (肝炎) 근섬유아세포의 (筋纖維芽細胞) 동태 (動態)

김호각(Ho Gak Kim),정준모(J.Mo Chung),손윤경(Yoon Kyung Shon),곽정식(Jyung Sik Kwak),손태중(Tae Joong Sohn) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.3

N/A This study has been carried out to investigate the behavior and histologic origin of myofibroblasts, which are able to be found in the liver tissue of chronic active hepatitis. The biopsied liver tissues of patient were examined under the light and electron microscopes and studied through the immunohistochemical method. Through light microscopic examination, the cases were devided into four mild ones, eight moderate ones and four severe ones. On immunohistochemical study, all mild cases show negative findings to antibody to antiactin and positive findings were demonstrated in all moderate and severe ones. On electron microscopic study, myofibroblasts were found in each three cases of moderate and severe ones and they exhibit intermediate forms between smooth muscle cells and myofibroblasts having the hundles of microfilaments, wrinkled nuclei and basement membrane. Most of them are located in nearby areas of portal veins and in the periphery of these areas, proliferation of Ito cells and transformation of them into similar forms of myofibroblasts were noted. These findings suggest that the myofibroblasts are noted in the moderate and more severe cases of chrrniic active hepatitis showing marked fibrosis and they contribute to the fibrosis of portal areas. Besides, it is presumed that myofibroblasts of chronic active hepatitis are originated from Ito cells.

소아에서 말초신경병증의 임상적 고찰

이환석(Hwan Seok Lee),박우생(Woo Saeng Park),고철우(Cheol Woo Ko),손윤경(Yoon Kyung Sohn),권순학(Soon Hak Kwon) 대한소아신경학회 2003 대한소아신경학회지 Vol.11 No.1

목적 : 소아에서 말초신경병증의 임상적 양상을 분석해봄으로서 미흡한 국내 자료에 보탬이 되고 각 질환들의 조기진단 및 조기치료에 도움을 주고자 본 연구를 실시하게 되었다. 방법 : 2000년 1월부터 2002년 12월까지 만 3년간 경북대학교병원 소아신경클리닉에서 말초신경병증의 임상적 증상이 있거나 인슐린 의존형 당뇨병 환자로 내분비 클리닉에서 추적 관찰하는 아이들 가운데 신경생리검사에서 비정상 소견을 보여 말초신경질환을 가지고 있을 가능성이 많은 62명의 아이들을 대상으로 후향적 조사를 실시하였다. 이 가운데 임상소견 및 검사소견을 근거로 말초신경병증이 있는 것으로 생각되는 39명을 환자 군으로 분류하여 자료를 정리 및 분석하였고 신경생리검사에는 이상이 있었으나 임상적 이상이 없었던 대부분의 당뇨병 환자(subclinical diabetic neuropathy)는 제외시켰다. 결론 : 임상적으로 유의한 말초신경병증이 있는 39명(남 24명, 여자 15명; 평균연령 7.6±4.3세)을 병인에 따라 분류해 보면 염증성/면역성이 원인이 된 경우가 18례(Guillain Barre 증후군 5례, 만성 염증성 탈수초성 다발성 신경병증 1례, Bell 마비 12례)로 46%를 차지하여 가장 많았고, 유전성이 원인이 된 경우가 6례(유전성 운동감각 신경병증 3례, hereditary neuropathy with liability to pressure palsy 1례, 이염성 백질이영양증 2례)로 15%, vincristine을 포함한 항암제 치료와 관련된 경우가 3례(8%), 외상 2례(5%), 당뇨성 1례(3%), 원인 불명을 포함하여 기타 7례(23%)였다. 침범된 신경의 기능해부학적 혹은 임상적 분류를 했을 때 운동성 신경병증이 32례로 80%로 가장 많았고 이어서 혼합형 즉 운동 감각 신경병증이 6례로 15%, 감각성 신경병증 2례로 5%이었고 자율신경병증은 없었다. 손상된 신경의 수에 따른 분류를 보면 다발성 신경병증이 23례로 59%를 차지하여 가장 많았고, 이어서 단신경병증이 15례로 38%, 다단신경병증이 1례로 3%가 되었다. 병리조직학적으로 살펴보면 탈수초성 신경병증이 22례로 56%를 차지하여 가장 많았고 이어서 축삭성 신경병증이 12례로 31%, 혼합형이 5례로 13%가 되었다. 전기 생리학적 검사 결과를 비교해보면 정상치에 비해 탈수초성인 경우는 운동신경 전달속도가 감소되었고 활동 전위폭이 다소 감소된 소견을 보였으며, 축삭성인 경우는 운동 및 감각신경 활동 전위폭이 감소되고 운동신경 전달속도가 다소 감소된 소견을 보였고 각 군들간에 있어서는 운동신경 전달속도가 통계적으로 유의하게 차이가 있었다(P<0.05). 결론 : 염증성/면역성 말초신경병증이 소아에 있어서 가장 흔하였으며 유전성 신경병증 가운데에는 유전성 운동 감각 신경병증이 가장 많았다. 또한 염증성/면역성 말초신경병증이 주를 이루지만 치료가 가능한 경우가 약 46%를 차지했고 약물이나 사고 등과 같이 잠재적으로 발병이나 악화를 예방이 가능했던 경우가 36%이었다. Purpose : To analyze the clinical features of peripheral neuropathy in Korean children. Methods : A total of 62 children with acute flaccid paralysis, longstanding weakness of extremities, or abnormal electrophysiological studies, suggestive of peripheral neuropathy, were evaluated retrospectively from the hospital records. The subjects were recruited at the pediatric neurology and endocrine clinic, Kyungpook National University Hospital from 2000 to 2002 and they all went through neurological examination and electrophysiological studies with or without nerve biopsy. Results : Thirty nine children(Male 24 : Female 15; Mean age 7.6±4.3 years) were found to have clinical peripheral neuropathy. Inflammatory neuropathy(5 children with Guillain Barre syndrome, 1 children with chronic inflammatory demyelinating polyneuropathy, 12 children with Bell's palsy; 46%) was the most common, followed by hereditary neuropathy(4 children, 10%), Chemotherapy induced neuropathy(3 children, 8%), metachromatic leukodystrophy(2 children, 5%), trauma(2 children, 5%), diabetic neuropathy(1 children, 3%) and so on. Thirty two children had motor neuropathy(82%), six children had combined motor and sensory neuropathy(15%), two had pure sensory(5%), but nobody had autonomic neuropathy. With respect to the type of involvement, polyneuropathies constitute 59%(23 children), mononeuropathy simplex accounted for 38%(15 children), mononeuropathy multiplex was found in 3%(1 child). Based on electrophysiological studied and biopsy results, demyelinating neuropathy was seen in 22 children(56%), axonal neuropathy in 12 children(31%), combined neuropathy in 5 children(13%). Eighteen children(46%) were completely or almost completely recovered from the illness. Conclusion : Inflammatory neuropathy was the most common among the acquired neuropathies and hereditary motor sensory neuropathy was the most common among the genetic neuropathies. Treatable neuropathies took up 46%. Potentially preventable neuropathies accounted for 36%. Early diagnosis and early intervention may have significant impacts on the prognosis of peripheral neuropathy in children.

실험전 시신경손상에서 Methylprednisolone 의 효과

김인택(In Taek Kim),홍승표(Sung Pyo Haong),손윤경(Yoon Kyung Sohn) 대한안과학회 1997 대한안과학회지 Vol.38 No.9

구강편평상피세포암 조직에서의 Microsatellite 불안정성 및 p53 유전자 돌연변이 분석

최태호(Tae Ho Choi),정운복(Woon Bok Chung),홍수형(Su Hyung Hong),김진아(Jin A Kim),나선영(Sun oung Na),장현중(Hyun Jung Jang),손윤경(Yoon Kyung Sohn),김진수(Chin Soo Kim),김정완(Jung Wan Kim) 대한구강악안면외과학회 2000 대한구강악안면외과학회지 Vol.26 No.4

Germ-line mutations at DNA repair loci confer susceptibility to colon cancer in hereditary non-polypopsis colorectal cancer. Somatic loss of DNA mismatch repair gene has been reported in a large variety of other tumor types. Replication errors(RERs) judged by microsatellite instability(MSI) and its associated mutations have been recognized as an important mechanism in various tumor types. To investigate associations between MSI and oral squamous cell carcinoma, the frequency of MSI using 12 microsatellite markers were analyzed for the series of oral tumors. Of 17 tumors, 8 cases(47%) did not show instability at any of the 12 loci; 5(29%) showed instability at 2~3 loci; and 4(24%) showed instability above 4 loci. The 4 cases showing widespread MSI did not differ from those without evidence of instability in terms of age at diagnosis, degree of differentiation, metastasis to lymph node, tumor location or the presence of mutations in the p53 tumor suppressor gene. DCC and D17S 796 were the most frequently detected in MSI analysis. There were no correlation between smoking and MSI frequency, instead, smoking was suggested to increase the mutation rate of p53 and development of oral carcinomas.