http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
유방염에 이환된 젖소에 있어서 혈액 동태학에 관한 연구
손성기,허정호,이주홍,김충희,김곤섭,김종수,Son, Sung-gi,Heo, Jung-ho,Lee, Ju-hong,Kim, Chung-hui,Kim, Gon-sup,Kim, Jong-shu 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.3
Hemodynamic values were assessed in the cows diseased with mastitis. Hemodynamic testes were performed for white blood cell(WBC), red blood cell(RBC), packed cell volume(PCV), hemoglobin concentration, monocyte, eosinophil, neutrophil, lymphocyte, prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, platelet concentrations, antithrombin-III and plasminogen activities. Significant alterations were observed in the mean values of most analytes. The numbers of monocytes, eosinophil, and neutrophil, and prothrombin time were increased while the number of lymphocyte, activated partial thromboplastin time, thrombin time, fibrinogen concentration, plasminogen activity and platelet concentration were decreased. The number of RBC, PCV, hemoglobin and antithrombin-III activity were unchanged compared with normal mean values. These data indicated that activation of hemodynamic mechanisms was initiated either directly by the endotoxin-releasing or indirectly by the inflammatory mediators released by response to etiologic agents. We suspected that the changes of hemodynamic values in the cows diseased with mastitis were very similar to those of experimental endotoxin-induced mastitis.
Diazinon이의 계배(鷄胚) 기형 유발에 미치는 영향 1. 계배(鷄胚)형태와 콜린성 봉쇄약물과의 관계
허정호 ( Jung Ho Heo ),손성기 ( Sung Gi Son ),이주홍 ( Ju Hong Lee ),김종수 ( Jong Su Kim ) 한국가축위생학회 1994 韓國家畜衛生學會誌 Vol.17 No.2
Teratogenic effects of diazinon were assessed morphologically and cholinergic blocking agents. Diazinon at doses ranging from 25 to 2000 ug/egg, was injected on day 3 of incubation. TD50s were different for the various teratogenic signs(wry neck, micromelia, abnormal feathering, abnormal beak and curled claws). The threshould dose for wry neck was higher than threshould dose for other signs; 40 ug/egg produced substantial micromelia, abnormal feathering. abnormal beak and curled claws, but gave no signs of wry neck. In contrast to the teratogenic doses, the LD50 of diazinon was very high(above 2000 ug/egg). One of the characteristics of diazinon-induced teratogenesis was reduced body weight(78.7%) and body length(73.8%). Maximal teratogenic effects, scored as signs of retarded growth, wry neck micromelia, abnormal feathering, abnormal beak, and curled claws, were produced when the insectcide was administered on the third or fourth day. The threshold dose for type II teratogenic signs(such as wry neck and short neck) was higher than for type I(such as micromelia and abnormal feathering). Morphological studies, using atropine and gallamine, suggested that nicotine but not muscarinic receptors may be involved in the mechanism of diazinon induced type II malformations.
유방염에 이환된 젖소에 있어서 혈액 동태학에 관한 연구
허정호 ( Jung Ho Heo ),손성기 ( Sung Gi Son ),이주홍 ( Ju-hong Lee ),김종수 ( Jong Shu Kim ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.2
Hemodynamic values were assessed in cows with naturally mastatis. hemodynamic tests included WBC, RBC, PCV, Hemoglobin, Monocyte, Eosinophil, Neutrophil, Lymphocyte, and prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, platelet, antithrombin-Ⅲ, and plasminogen activities. Significant changes were observed in the mean values of most analytses: WBC, monocytes, eosinophil, neutrophil were increased and lymphocyte were decreased. prothrombin time was increased: activated partial thromboplastin time, thrombin time. Increased: activated partial thromboplastin time, thrombin time, fibrinogen concentration, plasminogen activity and platelet concentration were decreased; and RBC, PCV, hemoglobin and antithrombin-Ⅲ activity were unchanged, compared with normal mean values. Thesse data indicated activation of hemodynamic mechanisms, initiated either directly by bacteria produced endotoxin of secondaly inflammatory mediators produced in response to caused bacteria and naturally acquired mastitis was very similar to the experimental endotoxin-induced mastitis.
Diazinon이 계배(鷄胚)의 기형 유발에 미치는 효과 I. 계배(鷄胚) 형태와 콜린성 봉쇄약물에 미치는 효과
김종수,김곤섭,김양미,최원영,손성기,허정호,이주홍,Kim, Jong-shu,Kim, Gon-sup,Kim, Yang-mi,Choi, Wong-young,Son, Sung-gi,Heo, Jung-ho,Lee, Ju-hong 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.1
Teratogenic effects of diazinon were assessed on morphology of chick embryos cholinergic blocking agents. Diazinon at doses ranging from 25 to $2000{\mu}g/egg$, was injected on Day 3 of incubation. $TD_{50S}$, were different for the various teratogenic sings such as wry neck, micromelia, abnormal feathering, abnormal beak and curled claws. The threshold dose for wry neck was higher than the threshold dose for other signs; $40{\mu}g/egg$ produced substantial micromelia, abnormal feathering, abnormal beak and curled claws, but gave no sings of wry neck. In contrast to the teratogenic doses, the $LD_{50}$ of diazinon was very high(above $2000{\mu}g/egg$). One of the characteristics of diazinon-induced teratogenesis was reduction of body weight(78.8%) and body length(73.8%). Maximal teratogenic effects, scored as sings of retarded growth, wry neck, micromelia, abnormal feathering, abnormal beak, and curled claws, were produced when the insectcide was administered on the third or fourth day. The threshold dose for type II teratogenic sings including wry neck and short neck was higher than for type I including micromelia and abnormal feathering. Morphorlogical studies, using atropine and gallamine, suggested that nicotinc but not muscarinic receptors may be involved in the mechanism of diazinon-induced type II malformations.
동물(젖소) 건강 Monitoring system 모델 개발 1. 경남 지역에서 우유내 항생재 잔류에 영향을 미치는 인자에 대한 역학적 평가
김종수,최민철,김곤섭,강호조,하대식,손성기,이종민,박일권,허정호,이주홍,안동원,Kim, Jong-shu,Choi, Min-cheol,Kim, Gon-sup,Kang, Ho-jo,Nah, Dae-sik,Son, Sung-gi,Lee, Jong-min,Park, Il-guen,Heo, Jung-ho,Lee, Ju-hong,An, Dong-won 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.3
A random study of 574 dairy farms in Gyeongnam area was designed to determine 1) management factors that may be associated with the occurrence of drug residues; 2) the dairy farmer's attitudes and knowledge about residues; 3) how these variables influence the occurrence of residues in dairy cattle. Management factors perceived as having the greatest influence on drug residues in milk were insufficient knowledge about withdrawal periods, errors due to hired help, insufficient identification and record of animals treated for mastitis, dry cow treatment for mastitis, and metritis treatment. Seventy-one percent of farms with residues problem used mixed own feeds compared with twenty-nine percent of farms with premedicated feeds. Factors significantly associated with the occurrence of residues were herd size, increased number of hired persons, increased frequency of use of mixed-own feeds, category of medicated feed, and producer's attitude toward the public health significance of residues. Our findings suggest that residue occurrence was mainly associated with errors due to hired help, insufficient knowledge about withdrawal periods, poor animal identification and records of treatment animals and use of medicated feeds. Any residue avodiance educational program needs to stress how to deal with these factors. This educational program should be directed to dairy farmers and employees, especially temporary employees. In addition, Dairy farmer's attitudes and knowledge about drug residues need to be improved. More evidence on the public healths significance of residues should be available to them. Because belife in importance of public healths concerns was related to successful residue avidance and because 81.3% of the dairy farmers with residue problem thought public healths concerns were less important than economic ones, it would be helpful to provide educational programs specifically directed to this issue. it may be useful to provide programs not only for the dairy farmers but also change of their concerns about on the public healths.
시료고체상분산처리와 액체크로마토그라피를 이용한 소의 근육, 간 및 천엽에서의 벤지미다졸계 구충제 잔류분석
김충희,김곤섭,박정희,하대식,류재두,손성기,허정호,정명호,김종수,Kim, Chung-Hui,Kim, Gon-Sup,Park, Jung-Hee,Hah, Dae-Sik,Ryu, Jae-Doo,Son, Sung-Gi,Heo, Jung-Ho,Jung, Myung-Ho,Kim, Jong-Shu 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
Simultaneous multiresidue analysis using liquid chromatography determination for five benzimidazole anthelmintics(thiabendazole, oxibendazole, albendazole, mebendazole and fenbendazole) in bovine muscle, liver and omasum has been described. Blank or benzimidazole-fortified samples(0.5g) were blended with bulk $C_{18}$($40{\mu}m$, 18% load, endcapped, 2g). A column made from the resultant $C_{18}$/animal tissue matrix was first washed with hexane($8m{\ell}$), following which the benzimidazoles were eluted with acetonitrile($8m{\ell}$). Analytes of extracted sample were determined by liquid chromatography with UV detector at 290nm. Correlation coefficients of standard curves for individual benzimidazole isolated from fortified samples, using internal standardization, were linear($0.991{\pm}0.007$ to $0.996{\pm}0.005$) with average relative percentage recoveries from $62.1{\pm}3.8(%)$ to $92.3{\pm}7.5(%)$ for the concentration range($0.2{\sim}6.4{\mu}g/g$), respectively. Recoveries rates of TBZ, MBZ in liver, OBZ, MBZ in muscle and TBZ, MBZ in omasium from fortified benzimidazole were 92.%, 87.3%, 74.5%, 82.7%, 75.2% and 83.5% at condition II, respectively. Condition II showed higher recoveries rates than condition I. These results indicated that the matrix solid phase dispersion(MSPD) methodology is acceptable for the determination of 5 benzimidazole anthelmintics and may also suitable for other matrixes of food animal origin.
LC/MS/MS를 이용한 식용란 중 fluoroquinolone계 항균물질의 분석법에 관한 연구
최유정 ( You Jeong Choi ),윤이란 ( I Ran Yun ),남상윤 ( Sang Yun Nam ),박영호 ( Young Ho Park ),김병훈 ( Byeong Hun Kim ),손성기 ( Seong Gi Son ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
An atmospheric pressure chemical ionization(APcI) LC/MS/MS method was developed for the simultaneous analysis of fluoroquinolones(norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin) residues in eggs. The spiked and blank samples were extracted from whole eggs using 50mM phosphate buffer(pH7.4). The extract was cleaned up by passage though Oasis(R) MAX extraction cartridge for solid-phase extraction followed by elution with 4% formic acid in methanol. The extract of sample was separated on a Waters AtlantisTM dC18 reversed-phase column(4.6×150㎜, 5㎛) and analyzed by APcI positive mode mass spectrometry. The mobile phase consists of aqueous 0.2% nonafluoropentanoic acid(NFPA) and methanol. Multiple reaction monitoring(MRM) using the precursor to product ion combinations of m/z 320 → 302, 332 → 314, 360 → 342 and m/z 358 → 340 were used to quantify norfloxacin(NOR), ciprofloxacin(CIP), enrofloxacin(ENR) and danofloxacin(DAN), respectively. The limits of quantification(LOQ) were 7.8ppb for NOR, 8.5ppb for CIP, 8.9ppb for ENR, and 4.8ppb for DAN. Average recoveries of fortified sample at levels of 0.025 to 0.1ppm were estimated 71.29% for NOR, 75.27% for CIP, 85.51% for ENR and 81.22% for DAN. These results could be applied for the confirmation and quantification in eggs.
Mouse에서의 Deoxynivalenol이 면역글로브린에 미치는 영향
이국천 ( Kuk Cheon Lee ),이주홍 ( Ju Hong Lee ),손성기 ( Sung Gi Son ),주영국 ( Young Kuk Joo ) 한국가축위생학회 1992 韓國家畜衛生學會誌 Vol.15 No.2
Mice were fed semi-purified diets containing 0, 2, 10 and 25 ppm(mg/kg) deoxynivalenol over 8 weeks and were assessed for effects on bodyweight gain, serum immunolgiobulin levels and surface immunoglobulin bearing lymphocyte ratio. 1. The rate of body-weight gain was significantly reduced (p<0.05) at the 10 and 25 ppm of DON, whereas the mice ingesting the diet containing 2 ppm DON was not. 2. IgA in serum immunolgiobulin was significantly increased (P<0.05) at the 10 and 25 ppm of DON, but IgG, IgM were decreased, whereas exposure to 2 ppm DON was not change. 3. Concentration of IgA from Peyer`s patch of mice fed DON exhibited increased at 10, 25 ppm. 4. Lymphocytes surface marker studies revealed that IgA, IgG and IgM were 2.2%, 0.4% and 1.5% respectively. These results suggest that dietary exposure to DON alters regulation of IgA production.