Factors Influencing Siderophore Production by Plant Growth Promoting Rhizopseudomonas Strains

성기영,Seong, Ki-Young 한국토양비료학회 1995 한국토양비료학회지 Vol.28 No.3

본 논문에서는 철 이외에 사이데로포 생성에 관여하는 요인을 연구하였다. 약산성(pH6)에서 세포의 양은 감소하는 반면에 세포당 사이데로포의 생성은 증가하였다. 사이데로포 생성의 적정온도는 7NSK2 균주가 $12^{\circ}C$이고 ANP15은 $19^{\circ}C$였다. 배지의 C : N 비율에 따른 사이데로포 생성력을 보면 C : N율이 낮은 배지에서는 사이데로포 생성과 세포생장은 감소됐다. 서로 다른 배지에서 사이데로포 생성을 조사한 바에 의하면 citrate를 탄소원으로 했을 경우 세포의 철 흡수능력이 증가했으며 사이데로포 생성도 감소되었다. 그러나 Glucose나 Succiuate에 비해서 citrate 배치의 세포량에는 별 차이가 나타나지 않았다. 이상의 결과로 보아 환경요인으로 작용할 수 있는 pH나 온도, 탄소원 등은 철분 외에도 사이데로포의 생성에 영향을 미칠 수 있음을 확인하게 되었다. This study demonstrates the influence of environmental conditions, other than iron, on pyoverdin production by fluorescent Pseudomosonas. In slightly acidic pH conditions(pH 6), cell yield was reduced while the siderophore production per cell yield was increased. The optimum temperatures for the siderophore production and cell yield was $19^{\circ}C$ and $28^{\circ}C$ for 7NSK2 and $12^{\circ}C$ and $19^{\circ}C$ for ANP15. The carbon and nitrogen balance showed that at low C : N ratio of the growth medium (higher nitrogen concentration), both cell yield and siderophore production was reduced. Use of different carbon sources revealed that citrate as a carbon source facilitated iron uptake and resulted in a significant reduction in siderophore production. However, at the late exponential phase, the iron content in the cell biomass was not significantly different from those grown in glucose or succinate. From these results it can be suggested that the environmental factors other than iron may also influence siderophore production by fluorescent pseudomonas.

극지환경성능시험설비를 활용한 저온환경 시스템 구축

성기영,한성종,이정희,Sung, Ki-Young,Han, Seong-Jong,Lee, Jung-Hee 한국산업융합학회 2022 한국산업융합학회 논문집 Vol.25 No.5

This paper was conducted to study the conditions for the manufacture and operation of artificial snow removal and ice-making test facilities so that the test equipment can be tested in a low-temperature environment using the polar environment performance test facility. The polar environment performance test Facility is designed to artificially simulate extreme environments up to -65 ℃, and is a mid-to-large low-temperature environment test facility that can perform performance tests on offshore plant equipment, ships, leisure, and offshore structures. To verify the safety of deck work of ships operating in polar environments, artificial snow removal and artificial ice making devices were manufactured, and we conducted research on various operating environments using these facilities. For the efficient operation of artificial snow and ice making facilities, it is important to continuously supply dry air, and it has been found that installing an additional heater at the tip of the nozzle is effective in preventing freezing.

형광성 Pseudomonas, SSL3 균주의 생존율에 미치는 Trehalose의 효과

성기영,류옥란,최원열,Seong, Ki-Young,Ryu, Ok-Ran,Choi, Won-Yeol 한국토양비료학회 2000 한국토양비료학회지 Vol.33 No.4

유용한 기능을 갖는 미생물을 농업현장에 쉽게 이용할 수 있게 하기 위하여 일반적으로 이들 균을 담체와 함께 건조시켜 입단화나 분말화 시킨다. 생체균을 건조하는 과정에서 미생물은 수분손실로 인해 삼투현상에 직면하며 거의 대부분이 사멸하고 만다. 본 연구에서는 높은 염농도나 건조에 의해 야기되는 균의 사멸을 감소시키기 위해 삼투보호제로 알려진 trigonelline이나 trehalose가 균의 생존에 미치는 영향을 조사하였다. 형광성 Pseudomonas, SSL3 균주의 염내성 농도는 5%가 한계농도이며, 7% 이상에서는 생장이 불가능하였다. 염농도가 높은 액체 배지에 trigonelline을 첨가하여 균의 생육을 조사한 결과 trigonelline은 배지의 종류나 염농도와 상관없이 균의 생장을 억제하는 효과가 있었다. 한편 trehalose를 액체배지에 첨가하면 10mM 농도이하에서는 4% NaCl을 포함한 LB 액체배지에서도 균의 생장이 증진되었으며 150mM이상의 농도에서는 균의 생장이 억제되었다. 균을 건조분말화시킬 때 trehalose를 첨가하여 토양중에서 균의 생존율을 조사한 결과 건조토양에서도 액체배지에서와 마찬가지로 trehalose를 첨가하면 첨가하지 않은 대조구에 비하여 균의 생존율이 증진되었다. Trehalose를 첨가한 제재화 균은 수분이 충분한 토양이나 건조한 토양 어느쪽에서든지 대조구에 비하여 균의 생존이 증진되었으며 특히 미생물 제제를 $-20^{\circ}C$나 $-70^{\circ}C$에 보관한 토양의 경우에 trehalose가 첨가된 제제의 생존율이 월등히 뛰어났다. FTIR의 분석결과 trehalose를 첨가한 4% 염농도에서 생장한 균에서는 대조구에 비하여 2차 amide기의 변화가 경감되었다. 이 결과에서 보듯이 trehalose는 염농도나 한발조건에 의해서 야기되는 균의 수분 스트레스로부터 균의 외막을 보호하여, 단백질구조의 급격한 변화를 감소시키므로 균의 생존율이 증진되는 것으로 사료된다. To make a better use of the beneficial bacterial inoculants in the agricultural practice, dry forms of bacterial fertilizer or pesticides are prepared with carrier materials. During the drying process of bacterial inoculant, most of the cells face a severe osmotic pressure and dehydration, and die off. Our study describes the effect of osmoprotectants such as trigonelline and trehalose on the survival of bacterial cells in high salt concentration and drying conditions. A fluorescent Pseudomonas, strain SSL3, used in this study, could grow in high salt concentration of upto 5% but the cells could not overcome the growth retardation at over 7% of salt concentration. The addition of trigonelline, even on small amount, in liquid medium containing 4% NaCl was detrimental to the cell. However, the addition of trehalose of upto 10 mM to the liquid medium containing 4% NaCl, enhanced cell growth. The cell growth was retarded when 150mM trehalose was added to the medium. Upon dry formulation of cells, trehalose was added. And the dry cells were inoculated into the soil to determine the effect of osmoprotectants on the survival of the cells. The survival of the cells, both in wet or dry soil, was improved by the addition of trehalose during the dry cell formulation. The positive effect of trehalose on the cell survival at $-20^{\circ}C$ and $-70^{\circ}C$ was oven more pronounced. The FTIR (Fourier transformation infra-red) spectroscopic analysis showed that the change of the 2nd amide group was reduced by adding trehalose to the medium containing 4% NaCl. These results suggest that trehalose can protect the cell membrane from dryness or high concentration of salt, thereby diminishing the sudden change of the protein structure of the cell membrane and, as a consequence, improving the cell survival.

Effect of Trehalose on the Viability of Fluorescent Pseudomonas, Strain SSL3

Ki-Young Seong(성기영),Ok-Ran Ryu(류옥란),Won-Yeol Choi(최원열) 한국토양비료학회 2000 한국토양비료학회지 Vol.33 No.4

Pseudomonas fluorescens ps88 이 생성하는 siderophore 가 병원균의 생물학적 방제와 식물생육에 미치는 영향

성기영(Ki Young Seong),신평균(Pyung Gyun Shin) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.1

Pseudomonas fluorescens ps88 was isolated from the rhizosphere soil produced the secondary metabolite called siderophore under iron Limited conditions. On iron limiting KMB medium this strain inhibited the growth of Pythium ultimum, Pyricularia oryzae, Rhizoctonia solani and Xanthomonas oryzae. Cucumber seeds were coated with the strain ps88 and were grown in green house soil. Forty days after the seed emergence, disease incidence caused by Fusarium oxysporium was reduced up to 50%. When the cucumber plants were grown in vermiculite, a significant fresh weight was increased. Root development of red pepper plants was also enhanced on MS medium supplemented with siderophore.

Factors Influencing Siderophore Production by Plant Growth Promoting Rhizopseudomonas Strains

Ki-Young Seong(성기영) 한국토양비료학회 1995 한국토양비료학회지 Vol.28 No.3

두과 월년생 잡초 살갈퀴의 녹비 이용성 검토

성기영(Ki-Yeong Seong),전원태(Weon-Tai Jeon),조현숙(Hyun-Suk Cho),김충국(Chung-Guk Kim),정광호(Kwang-Ho Jeong),송득영(Duk-Young Song),최봉수(Bongsu Choi) 韓國雜草學會 2010 Weed&Turfgrass Science Vol.30 No.2

자생 살갈퀴는 천립중이 14.9g, 종자크기가 2.8mm로 헤어리베치 27.7g, 3.41mm보다 작으며, 종자는 경실종자로 헤어리베치보다 휴면성이 높았다. 자생살갈퀴는 수원의 경우 4월초순이 되면 생장을 시작하며 무한화서로 개화기는 5월 5일경이며 6월 1일경에는 고숙기가 되어 종자채종이 가능하였다. 5월 중순이면 살갈퀴의 생장이 정지되는데 초장은 46.6~60.9cm이며 보리와 혼파를 하면 101.1cm까지 자랐다. 분지수는 2~6개이며, 착협 마디수는 분지당 3.5~3.7개, 협수는 3.8~6.6개였다. 살갈퀴 생체중은 2,960~14,450kg ha-1로 헤어리베치 24,860kg ha-1에 비해 크게 떨어졌다. 그러나 논에서 재배한 살갈퀴는 질소함량이 61~65kg ha-1(생체중 13,600~14,450kg ha-1)으로 화학비료를 절감하는 녹비로서 이용할 수 있을 것으로 생각되었다. 또한 살갈퀴는 사양질 논토양에서 재발생율이 높은 특성이 있어 이에 대한 연구 가 기대된다. Vicia angustifolia L. is native plant of winter annual leguminous weed in Korea. Objective of this research was to investigate the effects of V. angustifolia as green manure for rice production in sustainable agriculture. Seed size of V. angustifolia was smaller than that hairy vetch as control plant, while seed number in same amount was approximately 1.4-fold higher. V. angustifolia started stem elongation in early April. In addition, flowering and dead-ripe stages of the plant reached at May 5th and June 1st, respectively. Growth of V. angustifolia-barley mixed cropping system in upland was promoted up to 101 cm of plant length compared to 46.6 to 60.9 cm that grown in paddy. Biomass yield of V. angustifolia was 14.5 kg ha-1 in single cropping system of paddy soil, and the amount of nitrogen was 65 kg ha-1. Moreover, self-reseedling of fallen seed from V. angustifolia grown in previous year in paddy soil was significantly higher than that hairy vetch plant. These results suggested that V. angustifolia can be uses as green manure in addition to reducing the mineral fertilizer application in rice production for sustainable agriculture.

rDNA 의 ITS 2 부위의 염기서열분석에 의한 느타리 버섯 종간의 근연관계

배신철,성기영,이신우,고승주,은무영,이인구 ( Shin Churl Bae,Ki Young Seong,Shin Woo Lee,Seung Joo Go,Moo Young Eun,In Koo Rhe ) 한국균학회 1996 韓國菌學會誌 Vol.24 No.2

빙핵세균의 검출을 위한 특이적 항혈청 개발

이웅,권미경,성기영,조백호,김기청,Lee, Ung,Kwon, Mi-Kyung,Seong, Ki-Young,Cho, Baik-Ho,Kim, Ki-Chung 한국식물병리학회 1999 식물병연구 Vol.5 No.1

Frost injury of crops is closely related to the epiphytic population dynamics of ice nucleation-active (INA) bacteria, and the injury can be reduced by decreasing the INA bacterial population. In order to predict the epiphytic population of INA bacteria on crops, a rapid and accurate detection method has to be developed. In the previous report, we produced some antibodies against INA proteins purified from the outer membrane of INA bacteria. However it was difficult to produce the antibodies because the purification procedures of the INA proteins were complicated, and the final yield was too low. We designed a specific peptide from the N-terminal region of INA protein by computer analysis and synthesized the peptide in vitro in this experiment. The peptide sequence was Asp-Ser-Por-Leu-Ser-Leu-His-Ala-Asp, that is corresponding to the highly conserved region in several INA proteins, with predicted beta turn, coiling, and hydrophilic region. A polyclonal anti-INA peptide antiserum produced specifically recognized INA bacteria as few as 10 colony-forming units (CFU) in the ELISA reactions and did not respond to other non-INA bacteria. Serological specificity of the anti-INA peptide antiserum will facilitate the forecasting of the INA bacterial population dynamics on crops.

토양에서 분리된 Xanthomonas sp. 의 Chitinase 유전자 cloning 과 E.coli 에서의 발현

황철원,김호상,성기영,은무영 ( Cher Won Hwang,Ho Sang Kim,Ki Young Seong,Moo Young Eun ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.2

Xanthomonas sp. isolated from soil exhibited cell wall lytic activity of Candida albicans and secreted chitinase in chitin media. Especially, the chitinase activity was induced by chitin and reached a maximum level at 3 days culture in chitin media. We constructed genomic library of Xanthomonas sp. using cosmid vector in E. coli. Oligonucleotide probe, was synthesized from the consensus sequence corresponding to chitinase active site, which was derived from the comparison of amino acid sequences of bacterial chitinase genes. Using this oligonucleotide probe, we screened the genomic library. By restriction enzyme mapping of the positive clones, we identified 4 independent clones which may contain the chitinase gene. One of the clones, named pXCH1 (1.2 kb insert), was further analyzed. Northern blot analysis indicated that is transcripts, 1 kb and 0.8 kb, were induced by chitin. When the cloned gene was induced by IPTG in E.coli cell, chitinase activity which was secreted onto culture media was not observed. However, when the cell was disrupted by using sonicator and then centrifuged, the supernatant exhibited chitinase activity. SDS-PAGE of the supernatant indicated that about 35 kDa protein was induced by IPTG. From these results, it was concluded that the cloned DNA was one of the chitinase genes of Xanthomonas sp.