이상지질혈증과 치료제 연구개발 경향

설인찬 ( In Chan Seol ) 대전대학교 한의학연구소 2009 한의학연구소 논문집 Vol.18 No.2

Most of the cholesterol is synthesized by liver in the body while about one of third is taken via dietary. The main functions of cholesterol is to protect membranes in cell surface, avoid the arterial bleeding by hypertension, and prolong the life of erythrocytes, and so on. However, overload of cholesterol leads to arteriosclerosis associated with leading death cause. Lack of physical activity, emotional and environmental stress, and low intake of protein or vitamin E induce the unbalance between HDL- and LDL-cholesterol so become a basis of ischemic disorders in heart, brain and elsewhere in the body. So far, four major classes of medications for hyperlipidemia are HMG-CoA reductase inhibitors (statins), bile acid sequestrants, nicotinic acid, and fibric acids. The statins can lower LDL and levels triglyceride, but may induce myopathy and an elevation of liver enzyme levels. The bile acid sequestrants lower LDL levels and raise HDL levels with no effect on triglyceride levels but side effects of gastrointestinal (GI) distress, constipation, and a decrease in the absorption of other drugs. Nicotinic acid and fibric acids lower LDL and triglyceride levels with showing flushing, hyperglycemia, hyperuricemia, GI distress, and hepatotoxicity dyspepsia, gallstones, myopathy, and unexplained noncardiac death as adverse effects. Above western drugs lower cholesterol by 15 to 30% while all have notable adverse effects. In traditional medicine, hyperlipidemia is regarded as retention of phlegm and fluid disease. Etiology and pathogenesis of hyperlipidemia is basically based on Spleen-Deficiency and Phlegm-Stagnation, accumulation and stasis of -heat, and Qi & blood stagnation induced by Phlegm-damp, water-dampness, and blood stasis. Thereby, strengthening Spleen and dissolving Phlegm, clearing away heat and diuresis, and supplementing Qi and activating blood circulation are commonly used therapeutic methods for hyperlipidemia. The traditional herbal medicine, have been used for patients with CVA, hypertension or hyperlipidemia in Oriental hospital or Oriental clinic. The lock and key theory is used to develop most of western medicine, however many diseases are caused by mixed factors in body-complex system. We expect that Oriental pharmacological theory could be newborn as a novel drug showing high advantage of blood levels of lipidsand QOL of performance without side effects.

어깨통증의 한의학적 치료에 대한 국내 임상 연구 고찰

김찬영(Chan-young Kim),양지혜(Ji-hye Yang),채인철(In-cheol Chae),최인우(In-woo Choi),유주영(Ju-young Ryu),정은선(Eun-sun Jung),김윤식(Yoon-sik Kim),설인찬(In-chan Seol),유호룡(Ho-ryong Yoo) 대한한의학회 2020 대한한의학회지 Vol.41 No.3

db/db mouse에서 인진(茵蔯), 울금(鬱金), 산사(山査) 복합추출물의 항고지혈 및 항산화 효과

김현태,김윤식,설인찬,유호룡,Kim, Hyun-tae,Kim, Yoon-sik,Seol, In-chan,Yoo, Ho-ryong 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.3

Objective: This study was performed to investigate the effect of IUS (Inulsan, an extract of Artemisiae capillaris (茵蔯), Curcumae longae (鬱金), and Crataegi fructus (山査)) on anti-hyperlipidemia, anti-oxidation, and anti-inflammation.Method: We administered water extracts of Artemisiae capillaris, Curcumae longae, and Crataegi fructus for three weeks to db/db mice (C57BL/Ks), animal models induced with type 2 diabetes mellitus. Mice were divided into three groups: normal (C57BL/6J mice group), control group (db/db mice without administration of IUS) and IUS group (db/db mice treated with IUS). Then we measured total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride in the serum after the oral administration of IUS.Results: 1. IUS did not show any cytotoxicity in RAW 264.7 cells. 2. IUS decreased AST, ALP, and creatinine levelsand did not show any liver or renal toxicity in the db/db mice. 3. IUS increased DPPH and ABTS radical scavenging activity and decreased ROS production in RAW 264.7 cells. 4. IUS significantly decreased IL-1β, IL-6, and TNF-α production in RAW 264.7 cells. 5. IUS increased HDL cholesterol and significantly decreased total cholesterol and triglyceride in db/db mice. 6. IUS significantly decreased the atherogenic index and cardiac risk factor. 7. In contrast with the control group, fat infiltration in the liver and aorta decreased in IUS treated mice. The cell nucleus was located in the central area in H&E staining of liver. And endomembranes also were more thinner than the control group in H&E staining of aorta.Conclusions: These results suggest that IUS might be effective in the prevention and treatment of dyslipidemia.

인진(茵蔯), 울김(鬱金), 지실(枳實) 추출물(ACA)이 HepG2 세포에서 나타나는 이상지질혈증 관련 인자 발현 및 항산화에 미치는 영향

유주영,조현경,유호룡,설인찬,김윤식,Ryu, Ju-young,Cho, Hyun-kyoung,Yoo, Ho-ryong,Seol, In-chan,Kim, Yoon-sik 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.3

Objective: To investigate the effect of Artemisiae Iwayomogii Herba, Curcumae Radix, and Aurantii Fructus Immaturus complex extract (ACA) on dyslipidemia-related factor expression and anti-oxidation in HepG2 cells. Method: After treatment with ACA in the HepG2 cells, DPPH, ABTS radical scavenging activity, ROS production, and glutathione (GSH) production were measured. The free fatty acid, lipid peroxidation (MDA), ACAT1, and HMG-CoA reductase mRNA expression were measured in the HepG2 cells after treatment with ACA. Results: 1. DPPH, ABTS radical scavenging activity increased in an ACA concentration-dependent manner. 2. ACA significantly decreased ROS production in comparison to the control group. 3. ACA significantly increased glutathione production. 4. ACA significantly decreased free fatty acid and lipid peroxidation (MDA) in the HepG2 cells. 5. ACA decreased the mRNA expression of ACAT1 and HMG-CoA reductase. Conclusion: These results suggest that Artemisiae Iwayomogii Herba, Curcumae Radix, and Aurantii Fructus Immaturus complex extract (ACA) inhibits dyslipidemia-related factor expression and that it is effective in anti-oxidation. A future in vivo experiment with ACA is needed to investigate the effect on anti-dyslipidemia. It is expected that ACA is effective in anti-dyslipidemia and applied to cardiovascular disease, ischemic heart disease, stroke, etc.

가미자음강화탕(加味滋陰降火湯)이 SHR의 혈압에 미치는 영향

백경민,조현경,유호룡,김윤식,설인찬,Baek, Kyung-Min,Jo, Hyun-Kyung,Yoo, Ho-Rhyong,Kim, Yoon-Sik,Seol, In-Chan 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.1

Objective : This study was done to investigate the effect of KJT on hypertension. Methods : Spontaneous Hypertensive Rats were sensitized and challenged with Kamijaeumganghwatang(KJT) for 5 weeks. Experimental group was treated with $200mg/day/2m{\ell}$ of KJT orally and control group was treated with $2m{\ell}/day$ of normal saline instead. Results : 1. KJT significantly showed significant protection against cytotoxicity and toxicity in the liver and the kidney. 2. KJT significantly decreased the blood pressure in SHR. 3. KJT significantly decreased the levels of aldosterone in SHR. 4. KJT significantly decreased the levels of dopamine, norepinephrine and epinephrine in SHR. 5. KJT significantly decreased the levels of sodium and potassium in SHR, but it did not decrease the levels of chloride in SHR. Levels of calcium in SHR increased, but only insignificantly. 6. KJT significantly decreased the levels of IL-6 in SHR, and significantly increased the levels of IL-10 in SHR. 7. For histological effects, KJT dillated capillary vessels in the kidney, significantly decreased eosinophilic changes in heart cells, and significantly decreased endothelial damage in the aorta. Conclusion: These results suggest that KJT is useful in treatment of hypertension.

과루인약침(瓜蔞仁藥鍼)이 Ovalbumin-induced Asthma Mouse Model에 미치는 영향(影響)

김정현,백경민,이현의,김종원,오병열,조현경,유호룡,김윤식,설인찬,심재철,Kim, Jung-Hyun,Baek, Kyung-Min,Lee, Hyun-Eui,Kim, Jong-Won,Oh, Byeong-Yeol,Jo, Hyun-Kyung,Yoo, Ho-Rhyong,Kim, Yoon-Sik,Seol, In-Chan,Shim, Jae-Chul 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.3

Objectives : The aim of this study was to investigate the effect of Trichosanthis Semen Herbal-acupuncture(TS-HA) at Joksamni(ST36) on ovalbumin-induced asthma in mice. Methods : C57BL/6 mice were sensitized and challenged with ovalbumin(OVA) once a week for twelve weeks. The experimental group was treated with 1% concentrations of TS-HA at Joksamni(ST36) three times a week for the last eight weeks. Results : 1. The weight and total lung cells of the mice in the group treated with TS-HA decreased significantly compared with those of control group. 2. Total leukocytes and eosinophils in Bronchoalveoler-lavage fluid(BALF) of the mice in the group treated with TS-HA decreased significantly compared with those of control group. 3. Eosinophils in BALF of the mice in the group treated with TS-HA in photomicrographs decreased significantly compared with those of control group. 4. According to histological analysis of lung sections, adhension of collagen in TS-HA decreased significantly compared with that of control group. 5. The concentration of IgE, IL-4, IL-5 in BALF and IL-4, IL-5, IL-13 in serum of the mice in the group treated with TS-HA decreased significantly compared with that of control group. 6. The number of $Gr-^+/CD11b^+,\;CD11b^+,\;CD3e^-/CCR3^+,\;CD4^+,\;CD8^+\;CD3e^+/CD69^+,\;CD23^+/B220^+\;cells$ in the lungs of the mice in the group treated with TS-HA decreased significantly compared with those of control group. Conclusion : These results suggest that Trichosanthis Semen-herbal acupuncture at Joksamni(ST36) done on C57BL/6 mice is effective in part in relieving OVA-induced asthma in C57BL/6 mice.

강지통맥음(降脂通脈飮)이 항산화 활성과 고지혈(高脂血) 유발(誘發) 백서(白鼠)에 미치는 영향(影響)

차관배,안정조,조현경,유호룡,김윤식,설인찬,Cha, Kwan-Bae,An, Joung-Jo,Jo, Hyun-Kyung,Yoo, Ho-Rhyong,Kim, Yoon-Sik,Seol, In-Chan 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.3

This experiments was performed to determine the effects of Gangzitongmaekeum(降脂通脈飮 : GTE) on antioxidation activity and hyperlipidemia induced by hypercholesterolemic diet in mice. The results obtained were as follows : 1. GTE showed a safety in cytotoxicity and toxicity of human fibroblast cells and liver. 2. GTE showed DPPH scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory effect on reactive oxygen species (ROS). 3. GTE treated mice showed body and liver weight decrease, compared to the hyperlipidemia-induced control group. 4. GTE decreased total cholesterol and LDL cholesterol levels significantly, but HDL cholesterol levels not significantly. 5. GTE decreased triglyceride levels significantly. 6. Glucose levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. 7. Albumin levels in GTE treated mice were similar with the hyperlipidemia-induced control group. 8. The lipophagy in liver compared with the control group tended to be decreased in GTE treated mice. In the change of aorta, the cell was regular and boundary of vessel wall was clear, compared to the hyperlipidemia-induced control group. 9. TBARS levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. 10. The change of SOD and catalase activity significantly increased compared to the hyperlipidemia-induced control group. 11. ACAT mRNA level and HMG-CoA reductase mRNA levels in GTE treated mice significantly decreased compared to the hyperlipidemia-induced control group. Conclusion : These results suggest that Gangzitongmaekeum is effective in antioxidation activity and dietary hyperlipidemia-induced mice.

백부근(百部根) 약침(藥鍼)의 천식억제(喘息抑制) 및 면역조절효과에 대한 실험적 연구

허민,오영선,조현경,유호룡,김윤식,설인찬,Heo, Min,Oh, Young-Seon,Jo, Hyun-Kyung,Yoo, Ho-Rhyong,Kim, Yoon-Sik,Seol, In-Chan 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.6

The aim of this study was to investigate the effect of SR-HA(Stemonae Radix-herbal acupuncture) at Joksamni(ST36) on ovalbumin-induced asthma in mice. C57BL/6 mice were sensitized and challenged with OVA(ovalbumin) for 12 weeks(once a week). One of the two experimental groups was just treated with needle-prick on Joksamni(ST36) and the other group was treated with 1% concentrations of SR-HA at Joksamni(ST36) for the later 8 weeks (3times/week). The weight of lung of the mice group treated with SR-HA decreased significantly compared with those of control group, and significantly increased compared with those of normal group. The total cells of lung of the mice group treated with SR-HA decreased significantly compared with those of control group. Total leukocytes and eosinophils in BALF of the mice group treated wtih SR-HA decreased significantly compared with those of control group. Eosinophils in BALF of the mice group treated wtih SR-HA in Photomicrographs decreased significantly compared with those of control group. According to histological analysis of lung sections, adhesion of collagen in SR-HA decreased significantly compared with that of control group. The concentration of IgE, IL-4, IL-5 in BALF of the mice group treated with SR-HA decreased significantly compared with that of control group, and significantly increased compared with those of normal group. The concentration of IL-4, IL-5, IL-13 in serum of the mice group treated with SR-HA decreased significantly compared with that of control group, and significantly increased compared with those of normal group. The number of Gr-1+/CDl1b+, CO3-/CCR3+, CD4+, CD8+, CD3e+/CD69+ cells in the lungs of the mice group treated with SR-HA decreased significantly compared with those of control group. These results suggest that Stemonae Radix Herbal-acupuncture at Joksamni(ST36) in 057B146mice may be an effective part to OVA-induced asthma in C57BL/6 mice.

자액사(自縊死)와 익수사(溺水死)에 사용된 약물분석(藥物分析) 및 투여방법(投與方法)에 대(對)한 문헌적(文獻的) 효과(考察)

안정조,설인찬,An, Joung-jo,Seol, In-chan 대전대학교 한의학연구소 2001 한의학연구소 논문집 Vol.10 No.1

In the literatual study on on the herb med and the medication of Resuscitation(蘇生術) of Jayaeksa(自縊死) and Yiksusa(溺水死), the results were as follows; 1. As the single herb medication, gesim(桂心), sesin(細辛), johyub, chongyub(蔥萊)), banha(半夏), yukge(肉桂) etc. were used. 2. The $s{\breve{o}}ng$(性) of used medicines is mainly $ons{\breve{o}ng$(溫性) and $yuls{\breve{o}}ng$(熱性), the mi(味) is sinmi(辛味). 3. The $gwigy{\breve{o}}$ng(歸經) is all most $simgy{\breve{o}}gn$(心經) and $pyegy{\breve{o}}ng$(肺經). 4. The efficacy is geopung(祛風), geosub(祛濕) and geodam(祛痰) 5. On the method of herb medication, In Jayaeksa(自縊死), Chuibub(吹法), Guanbub(觀法) were most used, and In Yiksusa(溺水死), Guanbub(觀法) was most used.

담궐두통으로 변증한 두통환자 치험 1례 보고

백인경(In Kyoung Baek),유호룡(Ho Ryong Yoo),조현경(Hyun Kyoung Cho),설인찬(In Chan Seol),김윤식(Yoon Sik Kim) 한의병리학회 2013 동의생리병리학회지 Vol.27 No.1