Aflatoxin $B_1$이 계배 간조직의 미세구조에 미치는 영향

조자향,전향미,서숙재,Cho, Ja-Hyang,Cheon, Hyang-Mi,Seo, Sook-Jae 한국현미경학회 1996 Applied microscopy Vol.26 No.2

To investigate the effect of aflatoxin $B_1$, on survival rate and ultrastructure of liver during chick embryogenesis electron microscopic methods were used. After injection of aflatoxin $B_1$ into the yolk, ultrastructural changes in the liver of chicken embryo were observed. The results were as followed. 1. 12-day old chicken embryos were treated with single injection of aflatoxin $B_1$ with the dose of $0.0005{\mu}g,\;0.005{\mu}g,\;0.05{\mu}g,\;0.5{\mu}g,\;2.5{\mu}g,\;5.0{\mu}g$ each. Chicken embryos treated with the dose of $0.5{\mu}g$ of aflatoxin $B_1$ had survival rate of 22%. The embryos treated with $2.5{\mu}g$ of aflatoxin $B_1$ hardly survived. 2. Chicken embryos treated with $0.05{\mu}g$ of afatoxin $B_1$ had hatched in 30%, but once hatched, they all survived. 3. After administration of $0.05{\mu}g$ of aflatoxin $B_1$ into the 12-day old chicken embryo, the electron microscopic studies were examined during development stages. The nuclei of hapatocytes became irregularly shaped and the structures of endoplasmic reticulum were changed to spherical types at 20-day old chicken embryo. Also, mitochondria became to be dilated and severe fibrosis was induced in the cytoplasm. However, the hepatocytes became almost normal in 30-day old young chicken.

Immunohistochemical Localization and the Characteristics of Antigenic Component Inducing IgE and IgG Antibodies in Spirometra erinacei

Chang-Hwan Kim(김창환),Sook-Jae Seo(서숙재),Hong-Ja Kim(김홍자),Kee-Hoon Kwak(곽기훈) 대한의생명과학회 1996 Biomedical Science Letters Vol.2 No.1

Spirometra erinacei의 유충인 sparganum(metacercoid)에 감염되었을 때 호산성백혈구의 증가와 IgE 항체역가가 증가된다는 보고가 있다. 이 기생충에 감염되었을 때 IgG 항체와 IgE 항체를 유도하는 충체의 항원성분의 소재를 면역세포조직화학적 방법으로 충체의 성체와 유충에서 비교하였고, 또한 충체의 추출물을 SDS-PAGE와 EITB를 이용하여 IgG와 IgE 항체 유도 항원성분의 면역적 특성도 추구하였다. IgG와 IgE 항체 유도성분은 성체와 유충의 근층에서 공통적으로 분포되어 있었고, IgG 항원성분은 근층 뿐만아니라 외피층과 유조직층에서도 반응이 나타났으며, 성체의 수태편절에서는 자궁 내에 있는 충난의 표면에서도 반응이 나타났다. 충체의 외피층에서 항원성분을 면역황금 표지법으로 관찰한 결과, 충체의 외피층(tegument)에서 IgG 항원성분의 분포밀도가 IgE 항원성분의 밀도보다 컸다. 충체의 추출물 중 IgG, IgE 유도 항원성 단백질의 면역학적 특성을 비교하였다. 성체의 추출물의 43개 분회 중 21개 분획이 IgG 항원성분으로서 반응하였고, IgE 항원성분으로는 21개 분획에서 반응하였다. 이들 중 11개 분획(410, 304, 268, 174, 162, 116, 92, 86, 72, 62, and 59 kDa)에서 IgG와 IgE가 교차반응하였으며, 유충의 추출물의 36개 분획 중 IgG 항원성분으로 22개의 분획에서 반응이 나타났고, IgE 항원성분으로는 13개의 분획에서 반응하였으며, 이들 중 5개 분획(204, 116, 92, 79, and 59 kDa)에서 IgG와 IgE가 서로 교차반응하였다. Antigenic components reacting with IgE and IgG antibodies were localized in muscular layer of adult and of larva, sparganum. But the antigenic components inducing IgG were localized at tegument and parenchyma in addition to muscular layer in adult and sparganum. Also in sparganum, the surface of calcareous corpuscles of parenchyma showed immunoreactivity to IgG antibody. However antigenic components inducing IgE antibody were not localized in tegument and parenchyma, but in adult worm, we observed the immunopositive reaction at the lining of vitelline follicles in mature proglottis and on surface of egg shell within uterus of graved proglottis. By the method of immunogold-labelling, we observed the location of antigenic particles in tegument of sparganum. The density of antigenic particles inducing IgG was higher than that of antigen particles inducing IgE in syncytial tegument, tegument cells. A total of 43 and 36 protein bands were resolved from crude extracts of adult and sparganum, respectively, by SDS-PAGE. 34 bands from crude extracts of adult and larva were migrated to same positions. By EITB, 21 bands of 44 bands in adult were recognized with IgG antibody, and also 21 bands of 36 bands in sparganum. 13 bands of them were common antigenic components both in the adult worm and sparganum. Because 19 bands of 44 bands in adult worm were reacted with IgE antibody, they were IgE antigenic component. In sparganum, 13 bands were IgE antigenic components. 9 bands of them were common antigenic component inducing IgE antibody in both adult and sparganum. 3 bands of antigenic component recognized by IgE and IgG antibody were nonspecific antigen in both adult and sparganum of Spirometra erinacei.

Localization and Developmental Changes of Dopamine $D_1$ and $D_2$ Receptor mRNAs in the Rat Brain

김명옥,최완성,이봉희,조경재,서숙재,강성구,김경진,백상호,Kim, Myeong-Ok,Choi, Wan-Sung,Lee, Bong-Hee,Cho, Kyung-Jae,Seo, Sook-Jae,Kang, Sung-Goo,Kim, Kyung-Jin,Baik, Sang-Ho The Korean Society for Integrative Biology 1997 Korean journal of biological sciences Vol.1 No.3

Dopamine plays diverse roles in the fetal brain development and differentiation. However, the development of the dopaminergic neurons and its receptors has not been fully understood. In our studies, in situ hybridization and immunohistochemical methods were used to investigate the ontogeny of dopaminergic neurons and its receptor subtypes during the fetal development of the rat. In situ hybridization data showed that dopamine $D_1$ and $D_2$ receptor mRNAs were expressed in the ventricular and subventricular zones of ganglionic eminence, thalamus, hypothalamus, and cortical neuroepithelium on gestational day 13. Expression of dopamine $D_1$ and $D_2$ receptors during gestational days 17 and 19 reached the same or similar level of that in the adult brain. Expression of $D_1$ receptor mRNA preceded that of $D_2$ receptor mRNA in the early developmental stage, although this pattern was reversed with the sharp increase of $D_2$ receptor mRNA soon after. $D_2$ receptor mRNA was expressed in substantia nigra of mesencephalon of the fetal rat brain. However, $D_1$ receptor mRNA was not detected in substantia nigra. Our results indicate that dopamine might function in the fetal brain during the early gestational period.

Bacillus thuringiensis 내독소단백질이 미국흰불나방 조직의 단백질 합성에 미치는 영향

전향미 ( Hyang Mi Cheon ),강석권 ( Seok Kwon Kang ),서숙재 ( Sook Jae Seo ) 한국잠사학회 1995 한국잠사곤충학회지 Vol.37 No.1

Rhabditis sp.의 생장에 미치는 온도의 영향에 관한 연구

金昌煥,徐淑才 慶尙大學校 1986 論文集 Vol.25 No.2

Rhabditis sp.는 토양선충의 한 종류로 토양내의 유기물을 섭취하는 자유선충인데 광범위한 온도조건에서도 서식하고 있는 것으로 알려져 환경요소 중 온도가 Rhabditis sp.의 개체증식과 생장에 미치는 영향을 검토해 보고자 여러 온도 조건하에서 배양하여 개체의 생장과 증식수 및 대사작용에 관여하는 Esterase와 Acid phosphatase의 pattern 변화를 Electrophoresis를 통하여 조사한 바 다음과 같은 결과를 얻었다. 1. 20℃±4의 온도범위에서 배양된 Rhabditis sp.가 다른 12℃, 32℃ 온도하에서 배양된 Rhabditis sp.와 비교하여 증식수와 체형의 크기에 차이가 있었다. 2. Nanspecific esterase는 총 13개의 Isoenzyme band가 확인되었으며, 각 온도에 따라 12℃는 7개, 16℃·20℃·24℃는 각 6개, 28℃는 5개의 major band를 확인할 수 있었다. band 5번(Rf 0.58)은 전 온도범위에서 확인되었고 band 12번Rf 0.89)는 24℃온도에서만 확인되어 각 band에 따른 활성온도 범위가 각각 달랐다. 3. Acid phosphatase의 Isoenzyme pattern은 총 4개의 Isoenzyme major band 중 band 1. 2. 4. 번은 12℃에서만 major band이었고(Rf 0.14, 0.29, 0.33), band 3번(Rf 0.31)은 16℃ 이상의 온도에서 major band이었다. The present studies was carried out to investigate the influence of temperature on the rate of growth and on the multiplication of Rhabditis sp.. Rhabditis sp. was cultured at various temperature levels (12, 20, 24, and 28℃) and analysed by electrophoresis to investigated the isozyme pattern of nonspecific esterase and acid phosphatase. The results obtained were as follows. 1. Rhabditis sp. cultured at 20℃±4 showed differences in the rate of multiplication and body lengths, as compared with that at 12° or 32℃. 2. A total of 13 isoenzyme bands of nonspecific esterase was idendified and the numbers of major bands at esch temperature(12, 16, 20, 14 and 28℃) were 7, 6, 6, 6, and 5, respectively. Band 5 appeared at all temperatures tested, whereas band 12 appeared only at 24℃. 3. The patterns of acid phosphatase isoeozymes showed 4 major bands.. Out of 4 major bands band 1, 2 and 4 represented as major bands only at 12℃, whereas band 3 represented as major band at temperatures above 16℃. The results showed that the temperatures had effect on the activity of isoenzyme bands.

Spirometra erinacei의 發育에 따른 Alkaline phosphatase와 Lactate dehydrogenase의 酵素 組織化學的 硏究

朴裁弘,徐淑才,金昌煥 慶尙大學校 기초과학연구소 1990 基礎科學硏究所報 Vol.6 No.-

Spirometra erinacei의 제3기 유충 plerocercoid(Sparganum)를 전이숙주인 흰쥐와 종숙주인 고양이에 감염시켜서 회수한 sparganum과 성충의 alkaline phosphatase와 lactate dehydrogenase(LDH)의 분포 및 동위효소 pattern을 비교하기 위하여 효소조직 화학적 방법과 전기영동법을 이용하여 다음과 같은 실험 결과를 얻었다. 1. Alkaline phosphatase는 sparganum과 성체의 표피층과 표피근층에서 많은 분포를 보였으며 실질근층에도 상당한 분포가 있었다. Isozyme band pattern은 sparganum에서 2개 성체에서 4개가 분리 되었는데, sparganum과 성체 모두 2번 band가 major band였다. 2. LDH는 sparganum의 표피근층과 성체의 표피근층에 많이 분포되었고, sparganum의 실질근층과 표피층에는 거의 분포되지 않았다. LDH의 isozyme band pattern은 sparganum과 성체에서 각각 1개의 band가 분리되었다. This study was performed to investigate distribution and isozyme band pattern of Alkaline phosphatase and Lactate dehydrogenase on developing Spirometra erinacei (sparganum, adult) recovered from sparganum infected rats and cat. The result obtained were as follows ; 1. Alkaline phosphatase had a strong activity in the tegument and the epidermal musculature of sparganum and of adult, but no detectable level in the parenchymal musculature. 2. Two and four Alkaline phospatase isozyme bands were found in sparganum and adult respectively. of these bands, band 2 was major band in sprarganum and adult. 3. The high activity of Lactate dehydrogenase was found in the tegument and epider mal musculature of adult and epidermal musculature of sparganum, but no detctable level in tegumemt and parenchymal musculature of sparganum. 4. Only one lactate dehydrogenase isozyme band was detectable in sparganum and adult.

흰쥐에 있어서 Sparganum의 移行과 發育에 관한 硏究

金昌煥,徐淑才,崔朱卿 慶尙大學校 1985 論文集 Vol.24 No.1

This study was carried out to investigate the migration and development of the Sparganum in rats and the changes of haematological values in the hosts infected with Sparganum. The results obtained were summarized as follows: 1. The recovery rate of the Sparganum in the infected rats was 57 per cent on the autopical findings of the infected individual rats during observations from 2 hours to 32 days after infection with Sparganum. 2. The larval Sparganum was found in the digestive tract within two hours after administration with oral route and then, the worms began to penetrate and migrate via the abdominal cavity within 4 to 6 hours and the worms were found in the muscular tissues 2 days onwards after infections. 3. The infected Sparganum began to deveolp the size of the body from 4 day after infection and then, the size of the Sparganum was measured by 52.9mm long and 3.2mm broad on 32 days after infection . 4. In the haematological observation of the host the red blood cell values changed little, while the white blood cells were increased significantly during 2 days after infection and thereafter they were recovered to the normal values.

Studies on the Patterns of Haemolymph Proteins in Silkworm(Bombyx mori L.) during Metamorphosis

SEO, Sook-jae 慶尙大學校 1985 論文集 Vol.24 No.1

polyacrylamide gel 전기 영동법을 사용하여 누에(Bombyx mori L,)의 변태시기 중의 혈림프를 Plasma와 hemocytes로 분리한 후 그 단백질의 패턴을 비교 규명코자 하였다. 전 변태시기동안 plasma에서는 총 23개의 단백질 band가 분리되었고 hemocytes에서는 총 25개 단백질 band가 분리되었는데 약간의 정량적인 변화를 나타내었다. PAS반응, Sudan black B, Toluidine blue O를 이용한 조직화학적 검사결과 plasma와 hemocyte에서 각 15개, 11개의 glycoproetin, 각 9개의 lipoprotein이 확인되었고 acidic mucopolysaccharide는 hemocytes에서만 8개가 확인되었다. 또한 plasma에서 15개의 esterase, 5개의 tyrosinase, hemocytes에서 10개의 esterase, 6개의 tyrosinase가 확인되었다. Changes of haemolymph proteins during the metamorphosis were determined by means of polyacrylamide gel electrophoresis. The results by polyacrylamide gel electrophoresis showed at least 23 protein hands in plasma and 25 fractions in hemocytes with relatively constant pattern during the metamorphosis. By use of histochemical staining method, 9 lipoproteins, 15 glycoproteins, 15 esterases and 5 tyrosinases were separated in plasma while in hemocytes, 9 lipoproteins, 11 glycoproteins, 10 esterases, 8 acidic mucopolysaccharides and 6 tyrosinases were separated.

Characterization of A New Chromosomal Gene Which Maskes dsRNA Yirus Cytopathology in ski^- Saccharomyces cerevisiae

HWANG, BO HYE,SEO, SOOK JAE,LEE, HYUN SOOK 경상대학교 유전공학연구소 1991 遺傳工學硏究所報 Vol.10 No.-

The yeast L-A virus(4.6 Kb dsRNA genome) encodes the major coat protein and a "gag-pol" fusion minor coat protein that separately encapsidate itself and M₁, RNA satellite virus a 1.8 kb dsRNA encode a secreted protein toxin(the killer toxin). The yest chromosomal SKI genes, so named for the superkiller phenotype of mutants, prevent viral cytopathology by lowering the virus copy number. Thus, ski^-mutants are either temperature sensitivity(ts) or cold sensitivity(cs) for growth. Under electron microscope virus-infested ski^- mutants showed abonormal budding pattern. We transformed a ski2 virus infested mutant with a yeast bank in a high copy cloning vector and selected the rare healthy transformants for analysis. One type of transformant recovered from typical ski^- cold sensitivity though it remained superkiller. Elimination of the DNA clone from the ski2 strain eliminated this phenotype and introduction of the VPMs(Viral Pathology masking gene in Ski^- strain) DNA recovered from such transformants into the parent ski 2 strain or into ski3 or ski6 mutants gave the same phenotype. These transformants recovered from abnormal budding pattern. Compare of dsRNA contents in VPMs transfomed cell with control cell transformed only YEp13 vector, dsRNA transformed cells were not changed. Thus VPMs is a noe chromosomal gene which maskes dsRNA virus cytopathology in ski^- background. The 10 kb insert showed this activity in ski^- virus infested cells.

Immunohistochemical Localization and the Characteristics of Antigenic Component inducing IgE and IgG Antibodies in Spirometra erinacei

Kwak,Kee-Hoon,Seo,Sook-Jae,Kim,Chang-Hwan,Kim,Hong-Ja THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.1

SSpirometra erinacei의 유충인 sparganum(metacercoid)에 감염되었을 때 호산성백혈구의 증가와 IgE 항체역가가 증가된다는 보고가 있다. 이 기생충에 감염되었을 때 IgG 항체와 IgE 항체를 유도하는 충체의 항원성분의 소재를 면역세포조직화학적 방법으로 충체의 성체와 유충에서 비교하였고, 또한 충체의 추출물을 SDS-PAGE와 EITB 를 이용하여 IgG 와 IgE 항체 유도 항원성분의 면역적 특성도 추구하였다. IgG 와 IgE 항체 유도성분은 성체와 유충의 근층에서 공통적으로 분포되어 있었고, IgG 항원성분은 근층 뿐만아니라 외피층과 유조직층에서도 반응이 나타났으며, 성체의 수태편절에서는 자궁 내에 있는 충난의 표면에서도 반응이 나타났다. 충체의 외피층에서 항원성분을 면역황금표지법으로 관찰한 결과, 충체의 외피층(tegument)에서 IgG 항원성분의 분포밀도가 IgE 항원성분의 밀도보다 컸다. 충체의 추출물 중 IgG, IgE 유도 항원성 단백질의 면역학적 특성을 비교하였다. 성체의추출물의 43개 분획 중 21개 분획이 IgG 항원성분으로서 반응하였고, IgE 항원성분으로는 21개 분획에서 반응하였다. 이들 중 11개 분획(410, 304, 268, 174, 162, 116, 92, 86, 72, 62, and 59 kDa)에서 IgG 와 IgE 가 교차반응하였으며, 유충의 추출물의 36개 분획 중 IgG 항원성분으로 22개의 분획에서 반응이 나타났고, IgE 항원성분으로는 13개의 분획에서 반응하였으며, 이들 중 5개 분획(204, 116, 92, 79, and 59 kDa)에서 IgG 와 IgE 가 서로 교차반응하였다. Antigenic components reacting with IgE and IgG antibodies were localized in muscular layer of adult and of larva, sparganum. But the antigenic components inducing IgG were localized at tegument and parenchyma in addition to muscular layer in adult and sparganum. Also in sparganum, the surface of calcareous corpuscles of parenchyma showed immunoreactivity to IgG antibody. However antigenic components inducing IgE antibody were not localized in tegument and parenchyma, but in adult worm, we observed the immunopositive reaction at the lining of vitelline follicles in mature proglottis and on surface of egg shell within uterus of graved proglottis. By the method of immunogold-labelling, we observed the location of antigenic particles in tegument of sparganum. The density of antigenic particles inducing IgG was higher than that of antigen particles inducing IgE in syncytial tegument, tegument cells. A total of 43 and 36 protein bands were resolved from crude extracts of adult and sparganum, respectively, by SDS-PAGE. 34 bands from crude extracts of adult and larva were migrated to same positions. By EITB, 21 bands of 44 bands in adult were recognized with IgG antibody, and also 21 bands of 36 bands in sparganum. 13 bands of them were common antigenic components both in the adult worm and sparganum. Because 19 bands of 44 bands in adult worm were reacted with IgE antibody, they were IgE antigenic component. In sparganum, 13 bands were IgE antigenic components. 9 bands of them were common antigenic component inducing IgE antibody in both adult and sparganum. 3 bands of antigenic component recognized by IgE and IgG antibody were nonspecific antigen in both adult and sparganum of Spirometra erinacei.