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다공성 유리 슬릿을 가진 전기삼투펌프 기초 성능에 관한 연구
서상태(Sang Tae Seo),이호(Ho Lee),박철우(Cheol Woo Park) 한국가시화정보학회 2008 한국가시화정보학회 학술발표대회 논문집 Vol.- No.2
The basic concept and preliminary performance results of a miniaturized electroosmotic (EO) pump with diaphragms at both ends to divide working fluid and drug solution delivery part were presented in this study. The separation of an electroosmotic pumping liquid from a drug using diaphragms is mainly to have a freedom in choosing an electroosmotic pumping liquid and to achieve the optimal drug delivery, and, preferably its precise control. Porous glass frit material slit is employed as an central membrane to enhance electroosmotic pumping by reducing permeable gap space between glass frit. As a result, the absolute maximum flow rate increased as the pore radius and the slit thickness deceased.
서상태(Sang-Tae Seo),이중섭(Jung-Sup Lee),박종한(Jong-Han Park),한경숙(Kyoung-Suk Han),장한익(Han-Ik Jang) 한국원예학회 2005 원예과학기술지 Vol.23 No.4
28개의 항생물질에 대한 감수성을 10 종류(Acidovorax속 1종, Xanthomonas속 1종, Ralstonia속 2종, Burkholderia속 1종, Agrobacterium 속 2종, Clavibacter속 1종, Erwinia속 2종)의 식물병원세균에 대해 감수성을 실내실험으로 조사하였다. 항균활성 조사는 two-fold agar assay로 실시하였는데, 모든 시험 균주는 cloxacillin과 oxacillin에 대하여 강한 저항성을 나타내었다. 또한, 대부분의 균주는 sulfisoxazole, triple sulfa, bacitracin, penicillin에 대하여 저항성을 나타내었다. 그러나, 대부분의 균주는 tetracycline에 강한 감수성을 나타내었으며, doxycycline과 moxalactam 순으로 높은 감수성을 나타내었다. Tetracycline은 식물병원세균의 효과적인 방제를 위한 항생물질로 보이며, 이에 대한 추가 연구가 필요하다고 사료된다. Twenty-eight antibiotics were evaluated in vitro for sensitivity of 10 plant pathogenic bacterial strains. The strains belonged to the following species: Acidovorax avenae, Xanthomonas campestris, Ralstonia solanacearum, Burkholderia cepacia, Agrobacterium rhizogenes, Agrobacterium tumefaciens, Clavibacter michiganensis and Erwinia carotovora. The results of two-fold agar assay showed that all strains were resistant to cloxacillin and oxacillin. Additionally, some were resistant to sulfisoxazole, triple sulfa, bacitracin and penicillin. However, most strains were highly susceptible to tetracycline, doxycycline and moxalactam in decreasing order. Tetracycline seems to be an effective antibiotic, and further investigations to determine the method of treatment appear to be worthwhile.
서상태,오혜영,강하영,Seo, Sang-Tae,Oh, Hye-Young,Kang, Ha-Young 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.1
Effects of gamma irradiation on microbiological changes of Gorosoe sap were characterized during a post-irradiation storage at $4^{\circ}C$. The aseptically collected sap was irradiated and stored at $4^{\circ}C$ for 0 to 60 days and analysed for standard plate counts and 16S rDNA. There were significant differences in the total number of colony forming units(CFUs) of bacteria between irradiated and non-irradiated control sap. Bacteria of non-irradiated sap were present at levels of $1.5{\times}10^4{\sim}2.3{\times}10^8\;CFU/m{\ell}$, whereas no viable microbial cells were detected in sap after 10 kGy of irradiation during storage. According to the 16S rDNA sequence analysis, bacterial community structures decrease with time and the most abundant strain was Pseudomonas species. Our results suggested that gamma irradiation can be used to enhance the shelf-life of Gorosoe sap.
형태 특징 및 분자 분석에 의한 산철쭉 흰가루병균 Erysiphe izuensis 동정
조성은 ( Sung-eun Cho ),이상현 ( Sang-hyun Lee ),이선근 ( Sun-keun Lee ),서상태 ( Sang-tae Seo ),신현동 ( Hyeon-dong Shin ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.1
The Rhododendron powdery mildew fungus Erysiphe izuensis has been recorded throughout Asia, including China, Japan, Korea, and Russia. In Korea, E. izuensis has been identified based on morphological characteristics of the anamorph found on introduced Rhododendron spp. We here describe the first identification of E. izuensis collected from Rhododendron yedoense f. poukhanense in Korea. Morphological characteristics of the teleomorph as well as internal transcribed spacer sequences of the species are provided. Moreover, we describe the characteristics of the primary conidia and conidial surface pattern of E. izuensis for the first time.
Septobasidium sp.에 의한 구실잣밤나무 고약병의 분자학적 다양성 분석
이건우,서상태,차병진,한상섭,Geon-Woo Lee,Sang-Tae Seo,Byeongjin Cha,Sang-Sub Han 한국식물병리학회 2023 식물병연구 Vol.29 No.4
2020년 제주도 동백동산 내 구실잣밤나무 수피에 고약병과 관련된 Septobasidium sp.가 발견되었다. 분리한 균주는 습실 처리하여 새로 생성된 균사에 대한 genomic DNA를 추출한 뒤 internal transcribed spacer 및 small subunit rDNA 유전자에 대해 염기서열을 밝혔으며 polymerase chain reaction-based restriction fragment length polymorphism 분석을 통해 균주들간의 분자학적 특성이 조사되었다. 이 새로운 Septobasidium sp.은 기존에 알려진 고약병들과 형태학적 및 계통학적으로 다르게 나타나 새로운 종으로 보고한다. 또한 이 고약병은 관찰 당시 주변의 다른 수목들에서는 발생하지 않고 오직 구실잣밤 나무에서만 나타나는 특성으로 기주특이성이 매우 높다는 것이 확인되었다. In 2020, within the Dongbaekdongsan area in Jeju Island, a Septobasidium sp. associated with a felt disease in Castanopsis sieboldii (Makino) Hatus. ex T. Yamaz. & Mashiba was identified. The symptom included the presence of brown, thin, and silk-like mycelial mats attached to the tree's bark, displaying variations in size from large to small. To induce hyphal growth, the samples collected were incubated in a moist chamber, and the newly formed hyphae were subjected to genomic DNA extractions. The nucleotide sequences of the internal transcribed spacer and small subunit rDNA genes were determined, and molecular characteristics among the isolates were investigated through polymerase chain reaction-based restriction fragment length polymorphism analysis. This Septobasidium sp. exhibited distinct morphological and phylogenetic features compared to those that were previously reported in South Korea. Consequently, this strain is taxonomically classified as a provisionally novel species of Septobasidium. Furthermore, the observed felt disease exhibited a high degree of host specificity, as it was exclusively identified in C. sieboldii without occurrence in other tree species at the time of observation.
에탄올 처리에 의한 광릉긴나무좀(Platypus koryoensis) 유인목 효율 증대
손수연,이선근,서상태,Son, Su-Yeon,Lee, Sun Keun,Seo, Sang-Tae 한국응용곤충학회 2015 한국응용곤충학회지 Vol.54 No.4
참나무시들음병 매개충인 광릉긴나무좀(Platypus koryoensis (Murayama))(Coleoptera: Platypodidae)의 에탄올과 신갈나무 수피 추출물의 유인효과에 대하여 조사하였다. 유인트랩과 유인목 시험에서는 에탄올과 신갈나무 수피 추출물을 사용한 처리구가 대조구(무처리)보다 높은 유인효과를 나타내었으나, 두 유인제 간의 유의성은 찾지 못하였다. 유인목의 직경별 침입공 수를 비교한 결과, 침입공 수는 직경이 클수록 증가하였다. 유인목의 횡단면 상부와 하부 침입공 수를 비교한 결과, 상부보다 하부에 침입공이 많았고, 통계적으로 유의한 차이(p<0.05)를 나타내었는데, 이는 직사광선에 의한 건조가 적은 하부가 매개충의 먹이가 되는 미생물 번식에 유리하기 때문이라고 추정되었다. 결과적으로 신갈나무 수피 추출물보다 비용 면에서 경제적인 에탄올이 광릉긴나무좀 모니터링 및 방제에 효율적으로 활용될 수 있을 것이며, 신갈나무 수피 추출물은 효율을 높일 수 있는 계속적인 연구의 필요성이 있다. The attractant effect to Platypus koryoensis (Murayama), an insect vector of Korean oak wilt disease, was determined using a multi-funnel trap and trap logs treated with ethanol and ethanol extract of the bark of the Mongolian oak Quercus mongolica (Fisher). The number of entrance holes in ethanol and bark ethanol extract treatments was higher than that in the control (blank bottle). However, the number of entrance holes in ethanol and bark ethanol extract treatments was not significantly different. The number of entrance holes in the logs increased with increase in diameter of the logs. The lower part of the trap logs had significantly more number of entrance holes than that in the upper part (p < 0.05). The lower part of trap logs tends to be rarely exposed to direct sunlight resulting in desiccation of the logs, which in turn provides good conditions for microbial growth, a food source for P. koryoensis. The results show, that ethanol could be used effectively in the monitoring and maintenance of P. koryoensis because of the cheaper cost of ethanol than bark ethanol extracts, and thus, having a higher economical advantage. Further research of oak bark ethanol extracts is necessary to improve its efficiency.