하위문화에 나타난 고스메이크업의 연구 -존 갈리아노의 패션쇼 메이크업을 중심으로-

김희선 ( Hee Sun Kim ),강상모 ( Sang Mo Kang ),백선영 ( Sun Young Back ) 한국미용학회 2009 한국미용학회지 Vol.15 No.3

Fashion show make-up became more various and trendy and remarkable makeup became the main current. The expression in a fashion show or trend makeup shows diversity and unlimited expression that are completely different from the previous makeup form. In particular, make-up trends that were introduced in collections after the latter half of 2000s show indefiniteness of expression and free subjective expression and aesthetic value overcoming the concept of the existing traditional beauty. Goth style that is called `new gothic` of medieval style has newly appeared in 21st century. Goth Style tried to enter popular culture while appearing as trends for fashion and make-up at fashion collections. Accordingly, this study aimed to examine how characteristics of goth make-up style were reflected on subculture and pocused on John Galliano fashion show make-up. For the study, literature study was conducted using John Galliano fashion show books, Also goth image in John Galliano fashion make-up were analyzed using photos in fashion magazines and related website for demonstrative analysis.

2009년 패션쇼에 나타난 초현실주의 패션스타일 연구

김희선 ( Hee Sun Kim ),강상모 ( Sang Mo Kang ),백선영 ( Sun Young Back ) 한국미용학회 2011 한국미용학회지 Vol.17 No.5

In the modern society, fashion has become an instrument to establish the sense of self-expression in lives of contemporary human beings as a result of industrialization and mechanization, and at the same time it has evolved as one of the important types of arts. Fashion is the mirror of the age in which we live and especially it is closely connected with expressing the internal images of human beings. The purpose of this study is to analyze the influences that are reflected on the surrealistic characters by representative designers, Alexander Mcqueen, Martin Margiela, and Comme des Garcons. This study deals with the interrelationship of surrealism centering on Alexander Mcqueen, Martin Margiela, Comme des Garcons 2009 fashion shows which influenced surrealism and surrealism`s character. In other words, this study examines how surrealism affected their shows as the source of costumes and makeup, hair style and how it is accepted and expressed in their fashion shows. To achieve this purpose, this study makes use of the analysis of Alexander Mcqueen, Martin Margiela, Comme des Garcons 2009 fashion show photos. The surrealistic style will be led by various techniques and trends and development through new characteristics in multi-culture merged with various fashion and makeup styles.

냉동식품 중 Listeria monocytogenes의 분포 조사 및 시험방법에 관한 연구

백선영,곽효선,차진,박성국,임순영,김형일,박선희,김창민 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

최근 급속히 소비가 증가되고 :긴는 냉동식품을 대상으로 교 monoef09☞HfS의 오염도를 확인하오 분리균웨 특성 조사 및 신속 시험법을 확립하고자 서울, 부산 둥 5개 지역에서 판매 되고 있는 냉동식품을 구입하여 쿄 monoe-frgrnes를 분리, 동정하고 분리균에 대하여는 혈청형 조사 및 항생제 내싱 검사를 실시하였다. 냉동피자, 만두, 식육 및 수산물 가공품 둥 총 624건의 냉동식품을 구입하여 L uonoefof☞nog뫼 오염도를 조사한 결과 55건(9.5%)의 검체에서 t raonoofog☞oeg가 검출되첬으며 각 식품군별로는 냉동 만두 5.a%, 피자류 12.8%, 식욱가공품 10.4%, 해산물 기공품 9.1%, 기타 8.8%에서 양성률을 나타내었다. 분리균의 혈청형은 type 1이 87.5%를 나타내었으며 type4가 12.5%, 기타가 3.6%를 나타내었다. 항생제 내성 시험 결과 대부뚠의 항생제체 대해 감수석을 나타내었으나 nalidixic ac펀, polyrnyxin 8에 대하여는 저항성을 나타내었다. 또한 Hernolysin gene (fJy 1, ffy 2)과 Internalin gene (inf 1, inf2)의 mixed primer를 이용하여 최종 확인을 위한 신속하교 정확한 시험법인 multi-pleB PCR 분석법을 확립하였다. 표준균준 및 분리균의 DNA 추출을 위하여 Iysozyme (3mg/m쓰과 Proteinase (200fe/mf)처리 및 boiling을 싫시하였다. PCR 후 전기영동시 교 mortoofofeneg의 표준균주와 분리균은 446bp와 714bp band를 나타낸 반면 t innocua와 교. iuanovi 균주는 band를 나타내지 않았다. We surveyed the dfstribution of L. monocytogenes with five groups of frozen fooods. Six hundreds twenty four samples of frozen foods were randomly purchased in the market and tested. Fifty nine samples were ploved to be L. monocytogenes (9.5%) including kyoza(4.3%), pizza(12.8%), processed meat foods (10.4%), processed sea foods(9.1%), other frozen foods (10.5%). Serotypes of those isolates were; serotype 1(87.5%), type 4 (12.5%) and others (3.1 %). Also, isolates were tested for the antibiotic susceptibility. They were resistant to nalidixic acid, streptomycin, and polymyxin B and sensitive to ohlorarnphenicol, ampicillin, carbenicillin, tetracycline, tobramycin, gentamicin, kanamycin. neomycin, norfloxacin, ciprofloxacin, trimethoprim sulfamethoxazale. A multiplex-PCR method for rapid confirmation of L. monocytogenes was applied to the isolates.. We developed a new PCR technique to extract DNA form the cell. Treatmient of lysezyme (2mg/ml), proteinase (200㎍/m1) and boiling (15 min) were carried ou. hly 1, hly 2 and inl 1, inl 2 were used as primers. Standard strains and isolates of L. monocytobenes showed a specific amplified products of 719 bp and 446bp. These fragment was not shown with L. innocua andd L. ivanovii samples on agarose gel electrophoresis.

寄宿舍生의 給食營養 및 體位發達에 關한 調査硏究 : 全北地域 男子高等學校 學生을 中心으로

白善榮 圓光大學校大學院 1982 學位論叢 Vol.8-2 No.-

This thesis is designed to study the nutrition intake status of dormitcry students and the physical index of boys' senior high school of Jeon Bug provincial area. And it is also an attempt to find, define nutritional problems, and help in their growth and development through proper nutritional management. Three of boys' senior high schools in Jeon Bug provincial area have been selected as subjects and the nutritional survey was conducted for nine days in the period covered from July 14th to July 25th, 1981. The results obtained through this survey were; I. Feeding nutrition survey a) In the intake of energy, groups were divided into A, B, C and fed 2,240, 2,654 and 3,034 kcal respectively, but it was found out, compared with the recommended level 2,900 kcal, groups of A, B indicated as shortage and C group was surpassed. They obtained nutrition chiefly from carbohydrate. b) In the intake of protein, group in A, B, C were fed 74, 83, 125g respectively and out of total protein, animal protein contained 28.72%, 15.72% and 46.72%, but it was discovered, compared with the recommended level of 80g, A group indicated as stortage and B group showed as shortage of animal protein. c) In the intake of calcium, A, B, C group was fed 537, 446, 797mg respectively, but it was found out, compared with the recommended level of 100mg, all were recorded as shortage and in iron intake, A, B, C group was fed 11, 24, 40mg respectively, but A group showed as shortage. d) It was indicated that all vitamin was short, particularly vitamin Bz as great shortage. II. Physical growth and development a) The average height was 169, 167, 169cm respectively in A, B, C group, but B group was found to be below the standard level. b) The average weight was 55, 62, 64kg respectively in A, B, C group, but A group was found to be below the standard level. c) The average chest-girth was 88, 89, 92cm respectively in A, B, C group, and it was found as the order of group A<B<C in their chest-girth. d) The average sitting-height was 91, 90, 93cm respectively in A, B, C group, and it was found as the order of group B<A<C in their sitting-height. e) In the Relative weight, all was found to be above 35 and fine as normal state. f) In the Relative chest-girth, all was found to be normal. g) In the Relative sitting-height, it was found out that length of legs of C group was longer. h) In Rohrer index, it was found as the order of A<B<C and all maintained 1.2. i) In kaup index, all was found to be above 2.0 and indicated ordinary development state. j) The Vervaeck index was 87, 89, 92 respectively in A, B, C group and the nutrition status was found to be good as the order of A<B<C, but indicated that the nutr-ition status of A group was far below.

백신위해성평가사업(Ⅱ) : 생물의약품의 안전성 평가를 위한 A형 간염 바이러스(HAV)의 오염지표 수립에 관한 연구

김병국,백선영,신진호,김재옥,민경일,류승렬,민복순,김도근,박미경,안미진,이상건,이석호,박순희 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

생물의약품의 안전성을 확보하기 위하여 제조공정 증 바이러스 등의 위해 물질 제거 및 불활즉 공정과정의 확립이 필수적이며 이에 대한 평가를 위하여 감도와 특이도가 높은 오염물질의 검출 방법 확립이 매우 중요하다. 본 연구에서는 모델 바이러스로 A형간염바이러스fhepautis A virus :HAV)를 대상으로 real-tile PCR을 이용하여 실시간으로 정확하고 민감하게 바이러스를 검색하고 정량할 수 있는 시험법을 개발하였고 이로부터 바이러스의 오염지표를 수릴할 수 있는 기반을 마련하였다. 먼저 LightC?cBerT.4i(Roche)를 이용하여 바이러스를 검색하기 위한 real-time PCR의 최적 조건을 확림하였다. HAV의 겅색과 정량을 위한 표준 RNA는 flasmid PHAV/7으로부터 in uitro transcription을 수행하여 만들었다. 정제된 RNA를 Ix10'copies/#』부터 Ix10"copies/낄까지 계단 희석하여 표준바이러스로 사용하였다. Real-time PCR로 표즌 RNA의 유효성과 재현성을 검사한 결과 각 희석 단계 모두 표준편차가 0.3을 넘지 않았고 변이계수는 0.02를 넘지 않아 표준 RNA는 우수한 유효성과 재현성을 보여 주었다. 세포배양으로부터 녈리한 HAV를 표준 RNA를 이용하여 정량하고 혈액제제에 spiking실험을 수행하였다. 이 패 제젠로 인한 PCR반응의 간섭이 나타났으며 이것은 표준 aHA를 오염되지 않은 같은 제제로_희석하여 끊색과 정량에 사용함으로서 오차를 줄일 수 있었다. 이 실험에서 개발된 Lightcycler를 이용한 real-time PCR법에 의해 HAV는 Ix102 copies/re이하까지 검색이 가능하였다. 또한 제제에 바이러스를 spiking 한 경우에도 같은 민감도를 보였다. Real-time 정량 PCR방법은 표준 RNA를 이용하여 생물학적제제에서 바이러스의 검색과 정량에 매우 유용하게 이응할 수 있다. The establishment of the highly specific and sensitive detection and clearance method of contaminants such as virus in the manufacturing process is essential and important for the safety of biological products. In this study, the sensitive real-time detection and quantification method of Hepatitis A virus as a model virus using LightCycerTM(Roche, Germany) was established for the viral clearance validation and contamination index. First, the optimization of real-time PCR condition such as annealing temperature and magnesium ion concentration was performed. The standard RNA(sRNA) used as quantification standard was prepared from plasmid pHAV/7 by in vitro transcription and was serially 10-fold diluted from 1×107 to 1×102copies/㎕. The standard deviation and coefficient variation of sRNA was not more than 0.3 and 0.02 respectively. It showed pretty good efficacy and reproducibility. The purified RNA from cell culture was quantified using sRNA and spiked into blood products. To reduce the interference of product itself in PCR reaction, sRNA was diluted with uncontaminated product. The sensitivity of the assay was 1×102 copies and the similar results was obtained in spiked blood products. From the study, real-time quantitative PCR method can be used to detect and quantify viral contaminants with a standard RNA in biological products.

국내 사람 로타바이러스의 혈청형 항원부위의 염기서열 비교분석에 관한 연구

민복순,김재근,백선영,신진호,김재옥,민경일,류승렬,김병국,김도근,박미경,안미진,신계명,이석호,박순희 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

사람 로타바이러스는 영 ·유아 설사질환의 주요 원인체로 매년 1억 2천 5백만 명 이상의 설사 환자를 유발시키고 있다. 발병률이 높음에도 불구하고, 국내는 유행 사람 로타바이러스의 발병률과 특성에 관한 연구 자료가 미흡한 실정이다. 따라서 현재 외국에서 개발되어 시판되고 있는 사람 로타바이러스 진단제제와 향후 국내 ·외에서 개발될 백신 품질효능 평가시 많은 어려움이 있다. 이에 본 연구에서는2000년 1월에서 4월 사이에 서울, 대전 등 총 9개 지역에서 로타바이러스 감염이 의심되는 국내 영 · 유아 설사 환자를 대상으로 하여 총 가김물 30f건을 수거하여 실험에 사용하였다 가검물에서 사람로타바이러스 양성를을 조사하기 위하여 라텍스응짇법(L.AT), 효소면역법(ELISA),폴리아크릴아마이드 겔 전기영동법(PAGE), 핵산 증폭법(RT-PCR)시험을 시행하였다. 그 결과 LAT는 71.9%, ELISA 64.9%, PAG료 53.0%, VPf-RT-PCR 68.5%, VP4-RT-PCR 69.5%이었다. VP7-PCR과 LAT간의 일치도는 96.7%,'#P4-PCR과 LAT간의 일31도는 98.0%를 나타내었다. PAGE시험으로 group을 조사한 결과 총 302건 중 159건이 전세계적으로 사람에게 감염하는 A 잉cuP을 나타내었으며,1건은 극히 일부에서 발생하는 C: group을 나타내었다. RT-PfR에 의한 VP7 혈청형은 C2 type 50건, Gl type 44건, G4 type 26건이며, VP4의 혈청형은 PIB[41 type 줬건, P2A(6) type 47건, PIA(8) type 템건이었다. 현재 세계적으로 Gl type과 PIA[81type의 혈청형이 유행하는 것으로 알려져 있으나, 이와 다르fl 본 실험의 경우 G2 type과 PIB14T type이 가장 높은 경향을 나타내었다. Human rotaviruses are the leading cause of severe diarrheal disease of infants and children younger than 2 :fears of age worldwide. Thousands of children in less devetopedcountries died from rotavirus infecdon every year. Current research efforts are directed atimproving the effectiveness of rotavirus vaccines and reducing adverse effects of vaccination.It is expected that safer and more effective vaccines as weIB as a variety of diagnostic kitsincludhg serotyping Icits will be commercially available in the near future. In this study weinvestigated human rotavinls infection in Korean infants and young children who visil~edhospital due to diarrheal illness. A total of 302 fecal samples were collected from 9 urban areasacross the national terftory from January to April 2000. Delfction (If rotaviruses in fecalsamples was peformed using latex agglutination test (LAT), enzyme-linked immunosorbentassay (ELISA), polyacrylamide gel electrophoresis (PAGE), and reverse transcription-polymerasechain reaction (RT-PCR). G and P typug analysis was performed by multiplex PCR andsequencing. Of 30f samples tested and the positive rates were 71.9% (fA7), 64.9% (196), 53.0%(t60), 69.5% (211), and 68.5% (207) by LAT, ELISA, PAGE, VPT-PCR, and VP4-PCR,respectively The G(VP7) and P(VP4) typing and phylogenetic analysis revealed that Gl, C2,G4, PIA141, PIB[87, and P2A[6] types of gioup A rotavirus were prevailing in the givensampling frame during the study period. The most prevalent type was G2 and PIB[47 type. Itwas an unexpected finding in that the world-most prevalent type has been reported to be Gl,PIA[8B. A further study with greater sample size and expanded time period of sample collectionis necessary to confirm the difference.