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Sophorose의 제조 -Ⅱ. 효모 (Torulopsis bombicola) 배양액 및 회화나무 (Sophora japonica)로부터 Sophorose의 제조
백남인,이미자,이유희,박종대,김해영,김신일 ( Nam In Baek,Mi Ja Lee,You Hui Lee,Jong Dae Park,Hae Yeong Kim,Shin Il Kim ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.2
A yeast, Torulopsis bombicola, was cultured in the media fortified with soybean oil as a additional carbon source for 7 days with reciprocal shaking. From the culture broth, sophorose-lipid was isolated and treated with alkali to afford sophorose. The sophorose contained in the medium was acetylated and isolated through silica gel column chromatography. The aceylated sophorose was hydrolyzed with 5% KOH at room temperature to give rise to sophorose. Meanwhile, the MeOH extracts obtained from the pod of Sophora japonica was solvent-fractionated with n-BuOH and H₂O, and butanolic layer was chromatographed on silica gel column to afford a flavonoide-glycoside. The glycoside was hydrolyzed with 0.02 N H₂SO₄, to yield sophorose.
식용 식물자원으로부터 활성물질의 탐색-1 고구마 (Ipomoea batatas Lam .) 괴근 주요성분의 분리
백남인,안은미,방면호,김해영 ( Nam In Baek,Eun Mi Ahn,Myun Ho Bang,Hae Yeong Kim ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6
The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with EtOAc, n-BuOH, and H₂O, respectively. From EtOAc fraction four different compounds were isolated through the repeated silica gel column chromatographies. From not only the results of NMR and MS data but also the adaptation of hydrolysis, methylation, and acetylation, the chemical structures of compounds were elucidated as resin glycoside, simonin I, β-sitosterol, and two kinds of unsaturated fatty acids.
적변삼 외피에서 지질, 페놀성물질 및 철 성분의 축적에 관한 연구
이태수(Tae Su Lee),목성균(Sung Kyun Mok),천성기(Seong Ki Cheon),윤종혁(Jong Hyuk Yoon),백남인(Nam-In Baek),최정(Jyung Choe) 고려인삼학회 2004 Journal of Ginseng Research Vol.28 No.3
The study was conducted to investigate the tissue and chemical characteristics of rusty root epidermal cells. In histological study. the rusty symptoms were frequently observed in the epidermis of ginseng root and to be yellow under microscopic observation. Disks of the epidermal cell tissue of the rusty root were usually 2 and 3 times greater in the number of cell layer and thickness of cell wall than the healthy root. respectively. The color degree of methanol extracts li'om the rusty root epidennis was 5.5 times higher than that of the healthy root. And the extracts of rust matter in the root epidermis were easily dissolved in polar solvents compared to nonpolar solvents. UV-absorption spectra of methanol extracts in various fractions of phenolics showed a maximum peak between 275-280 nm. The crude lipids and phenolic compounds such as acid insoluble bound phenolics. acid insoluble esterified phenolics. acid insoluble condensed phenolics. insoluble bound phe-noli cs and free phenolics were also more in the rusty root epidennis than in the healthy one. Fe content in the rusty root epi-dermis was 2.7 times higher than that of healthy one. It was presumed that the phenolic compounds(precursor of the rusty) in association with lipid and iron in the root epidermis might defence the root when ginseng root was depressed by the unfavorable conditions in soil ancvor portions of a root system were subjected to anoxic conditions.<br/>
Sophorose 의 제조 - Ⅰ. D - Glucose 로부터 sophorose 의 화학적 합성
백남인(Nam In Baek),박종대(Jong Dae Park),이유희(You Hui Lee),김신일(Shin Il Kim),(Victor P . Anufriev) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.6
Sophorose (2-O-β-D-glucopyranosyl-D-glucopyranose) was chemically synthesized from D-glucopyranose through six steps of chemical reactions with the yield of 21%. The chemical structures of sophorose and some compounds obtained during reactions were confirmed by interpretations of spectral data, NMR, IR, etc.
2D - NMR 기법을 이용한 ( 2OS ) - 와 ( 2OR ) - ginsenoside Rh2 의 1H - 및 13C - NMR Signals 의 완전 동정
백남인(Nam In Baek),김동선(Dong Seon Kim),이유희(You Hui Lee),박종대(Jong Dae Park),정소영(So Young Jeong),이천배(Chun Bae Lee),김신일(Shin Il Kim) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.2
(20S)- and (20R)-Ginsenoside Rh₂ were prepared from crude ginseng saponin by chemical treatments. The ¹H- and ^(13)C-NMR signals of these compounds were fully assigned by various NMR techniques such as DEPT, ¹H-¹H COSY, HMQC, HMBC and NOESY.
답토양(畓土壤)에서의 시비규산(施肥珪酸)과 인산(燐酸)의 상호작용(相互作用)에 관(關)한 연구(硏究)
임선욱,백남인,Lim, Sun-Uk,Baek, Nam-In 한국토양비료학회 1983 한국토양비료학회지 Vol.16 No.4
답토양(畓土壤)에 시용(施用)한 규산(珪酸) 및 인산질비료(燐酸質肥料)의 토양중(土壤中)에서의 가급성(可給性)에 대한 상호간(相互間)의 작용(作用)과, 수도체(水稻體)에 의한 양성분(兩成分)의 흡수(吸收)에 미치는 상호관계(相互關係) 및 수도체(水稻體)의 생육(生育)에 미치는 영향(影響)을 시험(試驗)하기 위하여 수도품종(水稻品種) "진흥(振興)"에 규산질비료(珪酸質肥料)와 과인산석회(過燐酸石灰)를 각각(各各) 다른 수준(水準)으로 처리(處理)하여 재배시험(栽培試驗)한 결과(結果)는 다음과 같다. 1. 토양중(土壤中)에서는 규산(珪酸)과 인산(燐酸)은 상호간(相互間)에 길항적(拮抗的)으로 작용(作用)하여 두 성분(成分)의 유효도(有效度)를 저하(低下)시키는 경향(傾向)이었다. 2. 규산질비료(珪酸質肥料)의 시용량(施用量)이 많을수록 토양(土壤)의 pH가 상승(上昇)하였다. 3. 규산(珪酸)은 시용수준(施用水準)이 높을수록 수도체(水稻體)가 흡수(吸收)하는 규산(珪酸)의 양(量)은 증가(增加)하였으나 인산(燐酸)은 시용수준(施用水準)이 높아져도 체내(體內)의 인산(燐酸)의 양(量)은 증가(增加)하지 않았다. 4. 수도체(水稻體)에 의한 규산(珪酸)의 흡수(吸收)는 인산시용(燐酸施用)에 의해 뚜렷이 억제(抑制)되었으며 인산(燐酸)의 흡수(吸收)는 규산(珪酸)의 시용량(施用量)이 많을 때에는 뚜렷이 억제(抑制)되었으나 규산(珪酸)의 시용량(施用量)이 그보다 낮을 경우에는 생육시기(生育時期) 및 수도체(水稻體)의 부위(部位)에 따라 인산(燐酸)의 흡수(吸收)가 억제(抑制) 또는 촉진(促進)되는 경향(傾向)이었다. 5. 규산(珪酸)과 인산(燐酸)의 증시(增施)는 초장(草長), 분얼수(分蘖數) 및 이삭중(重)을 증가(增加)시켰고, 건물량(乾物量)은 일정(一定)한 경향(傾向)을 보이지 않았다. To investigate interactions of silicate and phosphate applied in paddy soil, different levels of the two fertilizers were treated on pots of Jinheung variety of Japonica rice, then availability and absorption of the silicate and phosphate and growth of the rice plant were analyzed. The availability of silicate applied in soil was reduced by increased application of phosphate and this antagonism implied the interaction of silicate and phosphate in soil. The silicate absorption by rice plants was remarkably reduced by the phosphate application, while the phosphate absorption was reduce only at high levels of silicate. At low levels of silicate, the phosphate absorption was affected differently depending on growth stage or parts of the rice plant. The application of silicate fertilizers increased the pH of the soils. The application of silicate and phosphate fertilizers improved the plant height, the tiller number, and the grain weight, but not the dry weight of the grain. The effect of interaction of silicate and phosphate on the plant growth did not appeared.
상추 (Lactuca sativa L.) callus로부터 ACAT 억제 활성물질, phytol의 생산
안광희,장태오,백남인,김세영,An, Kwang-Hee,Jang, Tae-O,Baek, Nam-In,Kim, Se-Young 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1
상추의 기내 callus 배양으로부터 ACAT (Acyl-CoA: Cholesterol acyltransferase) 억제 활성물질로 알려진 phytol 의 생산가능성이 연구되었다. Callus는 0.5mg.L$^{-1}$ NAA가 첨가된 MS 배지에 4주간 치상된 자엽 절편체로부터 유도되었으며, 다양한 수준의 chlorophyll을 함유하고 있었다. Phytol이 chlorophyll분자를 구성하는 물질로 알려져 있음에 도 불구하고, phytol의 추출량은 녹색의 자엽에서 보다 callus 에서 높게 나타났다. GC 분석결과, phytol의 추출량은 callus의 chlorophyll 함량과 비례하지 않는 것으로 나타났으며, callus의 chlorophyll 함량수준은 callus의 형성능과 관련이 있는 것으로 사료되었다. 본 연구의 결과는 phytol의 기내생산에 있어서 상추의 callus 배양의 잠재성을 제시한다 하겠다. The possibility for mass production of phytol, inhibitory diterpene against ACAT (Acyl-CoA: Cholesterol acyltransferase) was investigated by using callus culture of lettuce. The callus were induced from lettuce cotyledon explants on MS medium containing 0.5 mg.L$^{-1}$ NAA after 4 week's culture. Adventitious roots were formed from the explants on MS medium containing 0.5 mg.L$^{-1}$ IBA or 1.0 mg.L$^{-1}$ NAA. Adventitious shoots and roots were emerged from the callus when the callus was transferred to MS medium containing auxin alone, or with cytokinin. The plant growth regulators and their concentrations for the organogenesis were 1.0 mg.L$^{-1}$ NAA, 0.1 mg.L$^{-1}$ BA, 0.5 mg.L$^{-1}$ NAA with 0.1 mg.L$^{-1}$ kinetin, or 0.5 g.L$^{-1}$ 2.4-D with 1.0 mg.L$^{-1}$ kinetin. Analyses of chlorophyll contents showed that chlorophyll contents were higher in morphogenic calli than in non-morphogenic calli. However, the chemical analyses of gas chromatography indicated that phytol contents were not proportionate to the chlorophyll contents of callus. The content of phytol was higher in callus than in lettuce cotyledon.ledon.
이효정,길재호,백남인,라정찬,김성훈,Lee, Hyo-Jung,Khil, Jae-Ho,Baek, Nam-In,Ra, Jeong-Chan,Kim, Sung-Hoon 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3
Bio-Q is a modified prescription with the activities of supplementing Qi and blood in human body. In the present study, immunomodulatory effect of Bio-Q was examined. After oral administration of Bio-Q for 7 days to Balb/c mice, splenocytes were isolated and immunological experiments were performed. Bio-Q significantly increased the proliferation of splenocytes exposed to concanavalin A (Con A), while it did not in case of lipopolysaccharide (LPS) stimulation. Bio-Q also significantly increased CD3/CD19, CD4/CDB and NK cells by flow cytometric analysis. In addition, Bio-Q significantly enhanced the level of $INF-\gamma$ in splenocytes, but not $TNF-\alpha$ by ELISA. These results strongly suggest that Bio-Q has immunomodulatory activity through the regulation of T cell mediated immune pathway.