과립제와 환제 및 현탁액으로 만들어진 생약제제의 중금속 농도

배종섭(Jong-Sup Bae),박종필(Jong-Pil Park),김용웅(Yong-Ung Kim),박문기(Moon-Ki Park) 한국생물공학회 2010 KSBB Journal Vol.25 No.1

This study is an endeavor to evaluate the safety of medicines from heavy metals, prescribed on the basis of herbal medicinal system and oriental medical prescription which are circulated much recently. For that, six globular types, six granular types and seven liquid types of herbal medicines were bought to compare and analyze the content of heavy metals, such as As, Pb, Cd and Hg, which are harmful to human body. The concentration of As was 0.219 mg/kg to 1.243 mg/kg, Cd was 0.0282 mg/kg to 0.8481 mg/kg, Pb was 0.9582 mg/kg to 5.233 mg/kg and Hg was 0.001 mg/kg to 0.01 mg/kg in globular and granular types. Otherwise, in the liquid types, As was 0.0123 mg/kg to 0.5024 mg/kg, Cd was 0.0128 mg/kg to 0.0568 mg/kg, Pb was 0.1755 mg/kg to 0.712 mg/kg, and Hg was 0.001 mg/kg to 0.002 mg/kg. It was found that the concentration of heavy metals in liquid types herbal medicines was relatively lower than globular and granular types. It is required to treat, manufacture and manage herbal medicines for safety.

오배자 추출물의 Acetylcholinesterase 저해 및 항산화 활성 평가

배종섭 ( Jong Sup Bae ),이현식 ( Hyun Shik Lee ),이하영 ( Ha Yeong Lee ),유병혁 ( Byung Hyuk Yoo ),김태완 ( Tae Wan Kim ),김용한 ( Yong Han Kim ),김태훈 ( Tae Hoon Kim ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2012 한국식품저장유통학회지 Vol.19 No.5

Antioxidant capacity and acetylcholinesterase (AChE) inhibitory activity of the aqueous methanolic extract of Rhus javanica were investigated in vitro. The antioxidant properties was measured by radical scavenging assays using DPPH and ABTS+ radicals. The AChE inhibitory efficacy of R. javanica was tested by Ellman`s assay and the total phenolic content was determined using a spectrophotometric method. All tested samples showed a dose-dependent AChE inhibitory and radical scavenging activities. In particularly, ethyl acetate (EtOAc)-soluble portion from the methanolic extract of R. javanica showed significantly higher inhibitory activity than other organic solvent soluble-portions in an AChE and radical scavenging assay systems. These results suggest that R. javanica may be possess potential benefits which might be useful in development of antioxidant and anti-alzheimer`s disease ingredient.

생강 추출물의 pancreatic Lipase 저해 및 항산화 활성

배종섭 ( Jong Sup Bae ),김태훈 ( Tae Hoon Kim ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2011 한국식품저장유통학회지 Vol.18 No.3

Ginger (Zingiber officinale) is a well-known herb that is widely consumed as spice for the flavoring of foods. As part of our continuing search for bioactive materials, the in vitro pancreatic lipase inhibition and antioxidant properties of an aqueous ethanolic extract of Z. officinale were investigated. The total phenolic content was determined using a spectrophotometric method. The antioxidant efficacies of the extract was studied with radical scavenging assays using DPPH and ABTS+ radicals. Further more, the antiobesity effect of the extract was evaluated by porcine pancreatic lipase assay. In particularly, the pancreatic lipase inhibitory activity of the ethyl acetate (EtOAc)-soluble portion from Z. officinale was significantly higher than that of the other solvent-soluble portions. The results suggest that Z. officinale may have therapeutic potential that may be useful in development of an anti-obesity agent or its precursors.

엑스과립과 환으로 만들어진 한방생약제제의 aflatoxin B1 연구

배종섭 ( Jong Sup Bae ),김용웅 ( Yong Ung Kim ),박문기 ( Moon Ki Park ) 한국환경과학회 2010 한국환경과학회지 Vol.19 No.2

수용성 EPCR에 의한 활성화된 단백질 C의 항염증 작용에 관한 연구

배종섭 ( Jong Sup Bae ),박문기 ( Moon Ki Park ),박상욱 ( Sang Wook Park ) 한국화학공학회 2009 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.47 No.4

쑥부쟁이 추출물의 아세틸콜린에스테라제 저해 및 항산화 활성

배종섭 ( Jong Sup Bae ),김태훈 ( Tae Hoon Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4

Objectives: To evaluate the radical scavenging and acetylcholinesterase (AChE) inhibitory activities of the ethylacetate (EtOAc)-soluble portion of a methanolic extract of Aster yomena, three different assay systems were performed. Methods: The antioxidant activity of A. yomena extract was tested as its capacity to scavenging free radicals of DPPH and ABTS+, which has been widely used to evaluate the antioxidant activity of natural products from plant sources. AChE inhibitory activity was tested against mouse brain AChE by spectrophotometric method of Ellman using ELISA microplate reader. Results: The methanolic extract of A. yomena was fractionated and the EtOAc-soluble portion showed significant AChE inhibitory and free radical scavenging effects. Also the EtOAc-soluble portion revealed the highest phenolic contents as compared to the other extracts. Conclusions: These results indicate that phenolic compounds may be important constituents that give rise to the anti-AChE and antioxidative activities of A. yomena extract. Further phytochemical studies on this plant, for nutraceutical or pharmaceutical application, are warranted.

혈관내피세포에서 트롬빈이 TNF-α에 의해 유도되는 IL-6에 미치는 영향

배종섭(Jong-Sup Bae),박문기(Moon-Ki Park) 한국생물공학회 2010 KSBB Journal Vol.25 No.1

본 논문에서는 혈관내피세포에서 저농도의 트롬빈이 TNF-α가 NF-kB의 활성화를 통해 생성되는 IL-6의 생성량에 미치는 영향을 관찰하였다. TNF-α는 혈관내피세포에서 NF-kB의 활성화를 통해 염증을 유발시킨다는 것은 잘 알려진 사실이다. 이 논문에서는 TNF-α가 매개하는 염증작용에서 저농도의 트롬빈은 TNF-α가 생성시키는 IL-6의 생성량을 감소시켰고, 여기에는 트롬빈의 수용체인 PAR-1이 작용 하다는 것을 확인하였다. 뿐만 아니라, 세포내의 PI3-Kinase 역시 저농도 트롬빈이 관여한다는 것을 확인하였다. 이것은 저농도의 트롬빈이 수용체인 PAR-1을 활성화시키고, 활성화된 PAR-1은 PI3-Kinase의 활성화을 통해 항염증작용을 보여준다는 것을 의미한다. 이 결과는 향후 중증 패혈증 및 각종 염증질환을 치료할 수 있는 신약개발에 있어 중요한 단서를 제공하고 혈관내피세포에서 아직 명확하게 밝혀지지 않은 트롬빈의 염증작용 및 항염증작용의 기전을 밝히는 데 좋은 정보를 제공할 것이다. Here, we evaluated the effect of thrombin on the interleukin-6 production induced by tumor-necrosis-factor-α in endothelial cells. It is well known that tumor-necrosis-factor-α mediates inflammatory responses by activation of nuclear factor-kappa-B in endothelial cells. Here, we showed that lower concentration of thrombin decreased the production of interleukin-6 induced by tumor-necrosis-factor-α and this inhibitory effect of thrombin on interleukin-6 production was mediated by interacting with protease-activated-receptor-1. In addition, phosphoinositide-3-kinase was also involved the anti-inflammatory responses by lower concentration of thrombin in endothelial cells. These results suggested that lower concentration of thrombin mediated anti-inflammatory responses by interacting with protease-activated-receptor-1 on the cell membrane and phosphoinositide-3-kinase in the cell. These findings will provide the important evidence in the development of new medicine for the treatment of severe sepsis and inflammatory diseases and good clue for understanding unknown mechanisms by which thrombin showed the pro-inflammatory or anti-inflammatory activities in endothelial cells.

혈관내피세포에서 라이코펜이 염증유전자 발현에 미치는 영향

김태훈 ( Tae Hoon Kim ),배종섭 ( Jong Sup Bae ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2012 한국식품저장유통학회지 Vol.19 No.2

Lycopene, found in tomatoes and tomato products, has antioxidant, anticancer, and anti-inflammatory effects. High-mobility-group box 1 (HMGB1) mediates the pro-inflammatory responses in several inflammatory diseases. In this study, the potential roles of lycopene in the HMGB1-mediated pro-inflammatory gene expressions in the primary human-umbilical-vein endothelial cells (HUVECs) were investigated. The data showed that HMGB1 upregulated the expressions of monocyte chemotactic protein 1 (MCP-1), interleukin-6 (IL-6), secretory phospholipase A2 (sPLA2)-IIA, and prostaglandin E2 (PGE2). Lycopene pre-incubation for 6 h decreased the HMGB1-mediated induction of MCP-1, IL-6, sPLA2-IIA, and PGE2. Further study revealed that the inhibitory effects of lycopene on the HMGB-1 induced expression of pro-inflammatory genes were mediated by the inhibition of two important inflammatory cytokines: tumor necrosis factor (TNF)-α and nuclear factor (NF)-κB. These results suggest that HMGB1 upregulated the expression of pro-inflammatory genes and lycopene inhibited HMGB-1-induced pro-inflammatory genes by inhibiting TNF-α and NF-κB. This finding will serve as an important evidence in the development of a new medicine for the treatment of inflammatory diseases.

Effect of Activated Protein C (APC) on Apoptosis of Cancer Cells

Kyoung-jin Min(민경진),Jong-Sup Bae(배종섭),Taeg Kyu Kwon(권택규) 한국생명과학회 2012 생명과학회지 Vol.22 No.5

본 연구에서는 항응고제로서의 역할을 가지면서 또한 혈액응고와는 관련 없는 종양세포의 전이 등을 조절하는 것으로 알려진 activated protein C (APC)가 종양세포의 사멸에는 어떠한 영향을 미치는 지에 대한 연구를 수행하였다. Tumor necrosis factor (TNF)-α와 cyclohexamide를 병합 처리하거나 FAS를 처리하게 되면 인간 신장암세포인 Caki에서는 유의적인 세포사멸이 일어난다. 하지만, APC는 이러한 세포사멸에 아무런 영향을 미치지 못하였다. 또한 TRAIL을 인간 뇌 암세포인 T98G와 유방암세포인 MDA231세포에 처리하여 세포사멸을 일으켰을 때에도 APC는 세포사멸을 조절하지 못하였다. 그러나, TRAIL에 대한 민감도를 증가시키기 위한 kahweol과 TRAIL의 병합처리나, kahweol과 malatonin의 병합처리에 의한 신장암세포의 사멸은 APC에 의해 유의적으로 억제되는 것을 확인하였다. 따라서, 이는 APC가 항암치료의 효율성을 조절 할 수 있는 가능성을 가짐을 의미한다. Activated protein C (APC) has an anticoagulant effect and a non-hemostatic effect such as regulation of cell metastasis and modulation of inflammation. In this study, we investigated whether APC could modulate apoptosis in cancer cells. Tumor necrosis factor (TNF)-α , cyclohexamide, and FAS markedly induced apoptosis in human renal carcinoma Caki cells. When Caki cells were pretreated with APC, the percentage of death receptor-induced apoptosis did not change. Furthermore, we checked the effect of APC on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human glioma T98G and human breast carcinoma MDA231 cells. APC also had no effect on TRAIL-induced apoptosis in both cell lines. However, pretreatment with APC inhibited combination treatment (kahweol plus TRAIL and kahweol plus melatonin)-induced apoptosis and PARP cleavage in Caki cells. Taken together, our results suggest that APC can modulate anti-cancer therapeutic efficiency.

국내산 당근 품종별 carotenoid 함량 분석

하정림 ( Jeong Lim Ha ),배종섭 ( Jong Sup Bae ),박문기 ( Moon Ki Park ),김용웅 ( Yong Ung Kim ),하선화 ( Sun Hwa Ha ),배정명 ( Jung Myung Bae ),백경환 ( Kyong Whan Back ),이철호 ( Cheol Ho Lee ),이신우 ( Shin Woo Lee ),안미정 ( Mi 한국환경과학회 2009 한국환경과학회지 Vol.18 No.10

Carrots (Daucus carota L.) are consumed as an important dietary source of provitamin A including β-carotene, α-carotene and lutein. An HPLC method was applied to determine the content of the carotenoid composition in carrot cutivars cultivated in Korea. HPLC analyses were carried out with five carrot cultivars (Socheon-5-chon, Hongsim-5-chon, Myeongju-5-chon, Seonhongbom-5-chon and Betarich) sown at April, 2007 and six cultivars (Yeoreum-5-chon, Hanyeoreum-5-chon, Sinheukjeon-5-chon, Bibariheukjeon, Manina and Betarich) sown at August of the same year. In general, the former varieties are not used for the sowing at summer because of their bolting (growth of floral axis). The former and the latter carrots were harvested after 110 and 96 days from seeding, respectively, and the carotenoids were extracted with acetone after freeze-drying. The amount of α-carotene (117.7∼205.3 μg/g·DW) was similar to that of β-carotene (113.1~189.6 μg/g·DW) for the carrot cultivars sown at spring, while the content (46.2~71.1 μg/g·DW) was about a half of β-carotene content (92.5~140.2 μg/g·DW) for the latter cultivars. In addition, the average content of lutein (25.2 μg/g·DW) in the former cultivars was eight times higher than that in the latter cultivars (3.1 μg/g·DW). Among the spring cultivation types, Socheon-5-chon and Myeongju-5-chon showed higher amount of α-carotene and β-carotene, while the higher amount was determined in Yeoreum-5-chon and Sinheukjeon-5-chon among the autumn cultivation types. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the three compounds analyzed in a wide concentration range (0.025~20 g/ml). The R.S.D. values for intra-day and inter-day precision were less than 19.2% and the mean recovery of each compound was 85.4~104.7%.