소분지 대립 다수성 땅콩 신품종 ‘상안’

배석복(Suk-Bok Pae),황정동(Chung-Dong Hwang),이명희(Myung-Hee Lee),하태정(Tae-Joung Ha),심강보(Kang-Bo Shim),박장환(Chang-Hwan Park),백인열(In-Youl Baek),이종기(Jong-Ki Lee) 한국육종학회 2015 한국육종학회지 Vol.47 No.1

A new peanut variety ‘Sangan’ (Arachis hypogaea ssp. fastigiata L.) was bred at the Department of Functional Crop, National Institute of Crop Science (NICS), in Milyang 2011. This was developed from the cross between high quality cultivar ‘Palkwang’ and fewer branched germplasm ‘Chinese collect. 1’. ‘Sangan’ which is Shinpung plant type had 10 branches per plant and its length of main stem was 43 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel, and 100 seed weight was 96 g in the regional yield trials (RYT). Seed quality showed 48.5% of crude oil and 29.1% of protein content. This variety also showed more resistant to late leaf spot and web blotch, compared with reference cultivar. In the regional yield trials ‘Sangan’ was more productive than reference cultivar by 15% with 4.87 MT/ha for grain production.

조숙 다수성 풋땅콩 ‘아미’

배석복(Suk-Bok Pae),심강보(Kang-Bo Shim),김성업(Sung-Up Kim),이명희(Myung-Hee Lee),황정동(Chung-Dong Hwang),이춘기(Choon-Ki Lee),박장환(Chang-Hwan Park),백인열(In-Youl Baek),이종기(Jong-Ki Lee) 한국육종학회 2015 한국육종학회지 Vol.47 No.4

A new peanut variety ‘Ami’ (Arachis hypogaea ssp. fastigiata.) was bred at the Department of Functional Crop, NICS, in Milyang in 2012. This was developed by pedigree method after the cross between the short stem cultivar ‘Satonoka’ and the disease resistant cultivar ‘Milyang16’. ‘Ami’ which is Shinpung plant type had 10 branches per plant and its length of main stem was 50 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel, and its 100 seed weight was 84 g in the regional yield trials (RYT). ‘Ami’ showed 50.5% of crude oil and 60.5% of oleic acid composition in dry seed, and had 24.9 mg/g of sucrose and 4.5 mg/g of tannin content in fresh peanut. In the regional yield trials, ‘Ami’ was more productive than reference variety by 7% with 10.10 MT/ha for fresh pod production and by 14% with 4.51 MT/ha for dry grain production.

다분지 대립 다수성 땅콩 신품종 ‘신팔광’

배석복(Suk-Bok Pae),황정동(Chung-Dong Hwang),김성업(Sung-Up Kim),이명희(Myoung-Hee Lee),심강보(Kang-Bo Shim),박장환(Chang-Hwan Park),이춘기(Choon-Ki Lee),백인열(In-Youl Baek),이종기(Jong-Ki Lee) 한국육종학회 2016 한국육종학회지 Vol.48 No.1

A new peanut variety ‘Sinpalkwang’ (Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2012. This was developed from the cross between line ‘HP299’, crossed high yielding variety ‘Palkwang’ and short stem cultivar ‘PI156649’, and ‘Palkwang’. ‘Sinpalkwang’ which is a Virginia plant type had 25 branches per plant and its length of main stem was 41 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 45 pods per plant, 92 g of 100-seed-weight and 79% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 45.4% of crude oil and 29.2% of protein content. This variety also showed more resistant to web blotch, compared with reference variety. In the regional yield trials for 3 years ‘Sinpalkwang’ was more productive than reference variety by 28% with 5.4 MT/ha for grain production.

고 올레산 땅콩 신품종 ‘케이올’

배석복(Suk-Bok Pae),황정동(Chung-Dong Hwang),이명희(Myoung-Hee Lee),김성업(Sung-Up Kim),오기원(Ki-Won Oh),이병규(Byung-Kyu Lee),박장환(Chang-Hwan Park),박금룡(Keum-Yong Park),백인열(In-Youl Baek),전영춘(Young-Chun Jun) 한국육종학회 2016 한국육종학회지 Vol.48 No.3

A soybean cultivar for soy-paste, ‘Hwangkeumol’, was developed from the cross between SS92414 (crossing line of ‘Pokwangkong’ and ‘Suwon163’) and ‘Hwaeomputkong’ by soybean breeding team in the National Institute of Crop Science (NICS) in 2010. A promising line, SS99409-2B-11-5-4, was selected and designated as the name of ‘Milyang202’. It was prominent and had good result from regional adaptation yield trials for three years from 2008 to 2010 and released as the name of ‘Hwangkeumol’. It has a determinate growth habit, white flower, gray pubescence, yellow seed coat, yellow hilum, spherical seed shape and large seed (28.6 grams per 100 seeds). Maturity date of ‘Hwangkeumol’ was as early as September 15th. Therefore it is suitable for double cropping system. ‘Hwangkeumol’ was resistant to bacterial pustule and resistant to soybean mosaic virus, the major soybean diseases in Korea and tolerant to lodging in fields. The average yield of ‘Hwangkeumol’ was 2.51 ton per hectare in the regional yield trials carried out for three years from 2008 to 2010.

조숙 대립 다수성 풋땅콩 ‘보름1호’

배석복(Suk-Bok Pae),박장(Chang-Hwan Park),심강보(Kang-Bo Shim),하태정(Tae-Joung Ha),이명희(Myung-Hee Lee),황정동(Chung-Dong Hwang),박금룡(Keum-Yong Park),이학동(Hak-Dong Lee) 한국육종학회 2015 한국육종학회지 Vol.47 No.1

A new peanut variety ‘Boreom1’ (Arachis hypogaea ssp. fastigiata.) was bred at the Department of Functional Crop, NICS, in Milyang in 2010. This was developed by pedigree method after the cross between the large grain cultivar ‘Suwon 108’ and the short stem cultivar ‘Milyang4’. ‘Boreom 1’ which is Shinpung plant type had 9 branches per plant and its length of main stem was 43 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel, and its 100 seed weight was 105 g in the regional yield trials (RYT). ‘Boreom1’ showed 49.9% of crude oil and 54.5% of oleic acid composition in dry seed, and had 30.8 mg/g of sucrose and 4.2 mg/g of tannin content in fresh peanut. In the regional yield trials, ‘Boreom 1’ was more productive than reference variety by 8% with 10.16 MT/ha for fresh pod production and by 3% with 4.26 MT/ha for dry grain production.

소분지 대립 다수성 땅콩 신품종 ‘화선’

배석복(Suk-Bok Pae),이명희(Myoung-Hee Lee),김성업(Sung-Up Kim),황정동(Chung-Dong Hwang),오기원(Ki-Won Oh),이병규(Byung-Kyu Lee),이춘기(Choon-Ki Lee),박장환(Chang-Hwan Park),백인열(In-Youl Baek) 한국육종학회 2016 한국육종학회지 Vol.48 No.3

A new peanut variety ‘Huaseon’ (Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2013. This was developed from the cross between ‘Iksan 31’ with Virginia typed short stem and ‘SP9614’ with Shinpung-typed larger grain. ‘Huaseon’ which is a Shinpung plant type had 10 branches per plant and its length of main stem was 41 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 45 pods per plant, 84 g of 100-seed-weight and 79% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 47.3% of crude oil and 27.6% of protein content. This variety showed resistant to early leaf spot and had more resistant to stem rot and lodging, compared to reference variety. In the regional yield trials for 3 years ‘Huaseon’ was more productive than reference variety by 12% with 4.78 MT/ha for grain production.

다분지 고올레산 다수성 땅콩 신품종 ‘해올’

오은영(Eunyoung Oh),배석복(Suk-Bok Pae),김성업(Sungup Kim),김정인(Jung-In Kim),김민영(Min Young Kim),이정은(Jeongeun Lee),김상우(Sang Woo Kim),조광수(Kwang-Soo Cho),이명희(Myong-Hee Lee) 한국육종학회 2021 한국육종학회지 Vol.53 No.4

‘Hae-Ol’ (Arachis hypogaea ssp. Hypogaea) -a high-oleate peanut variety-was developed at the Department of Southern Area Crop Science,National Institute of Crop Science in 2018. This variety was selected from the pedigree line crossed between short stem Virginia-type ‘HP825’and high-oleate elite line ‘YG316’. The crude fat content of ‘Hae-Ol’ was 50.8%, and its oleic acid composition was 83.0% showing high-oleateproperty compared to that of ‘Daekwang’ variety (57.3%). The ratio of oleic acid to linoleic acid, which represents the oxidative stability, was12 times higher in ‘Hae-Ol’ than in ‘Daekwang’. The increments in acid value and peroxide value were relatively slower in ‘Hae-Ol’, under thecondition of accelerated oxidation. Over the three years of regional adaptation test (2016-2018) conducted at four regions, ‘Hae-Ol’ showed significantlyhigher grain yield (4.88 MT⋅ha-1), which was 16% more improved than that of ‘Daekwang’ (4.20 MT⋅ha-1). The seed of ‘Hae-Ol’ is brownin color and long-ellipse in shape and its 100-seed weight was 96 g. On field, ‘Hae-Ol’ demonstrated more resistance to late leaf spot (Cercosporidiumpersonatum) and web blotch (Phoma arachidicola). It also had a shorter branch and main stem, which was related to the higher tolerance to lodgingstress. The release of ‘Hae-Ol’ could contribute to the production of peanuts with enhanced quality and higher yield, and it is expected to be notablybeneficial to both the producers and manufacturers (Registration No. 8407).

연구보문 : 참깨 원산지에 따른 이화학적 특성 비교 분석

심강보 ( Kang Bo Shim ),황정동 ( Chung Dong Hwang ),배석복 ( Suk Bok Pae ),이명희 ( Myoung Hee Lee ),하태정 ( Tae Jung Ha ),박장환 ( Chang Hwan Park ),박금룡 ( Keum Yong Park ) 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.4

1. 참깨 생산 원산지에 따라 단백질, 세사민 함량은 유의적인 차이를 보였으나 기름 및 세사몰린 함량은 차이가 없었다. 2. 원산지에 따른 지방 함량은 원산지간 유의성은 인정되지 않았지만 한국, 미얀마, 베네쥬엘라, 중국 순이었다. 3. 원산지에 따른 단백질 함량은 한국, 수단, 인도, 베네쥬엘라 순으로 높았다. 4. 원산지에 따른 세사민 함량은 한국, 수단, 베네쥬엘라, 중국 순으로 높았다. 5. 원산지에 따른 참깨 종피의 물리화학적 비교에서 한국산 참깨는 세포조직이 치밀하고 둥근 모양이었으며, 왁스층이 두껍고 축적량이 상대적으로 많았다. 6. 본 시험을 통해 국산 참깨와 수입산 참깨를 구분하는 방법의 하나로 종자의 외형특성 이외에도 주요 성분함량이나 종피의 물리화학적 특성을 통해서 판별 할수 있는 기준이 될수가 있다고 사료되며, 향후 국산 참깨와 수입산 참깨를 성분함량이나 종자의 외형적 특성으로 차별화가 가능한 기준설정 연구가 필요 할 것으로 본다. To provide information on the phytochemical properties of sesame seed with different producing origin, the experiment which quantify and compare main components as well as physical properties of seed coat of imported and domestic sesame seeds were conducted. There was significantly different in protein and sesamin contents depending on the producing origins. Domestic sesame seed sample usually showed higher contents of oil, protein, sesamin and sesamolin than those of introduced ones. Total oil content of the imported sesame seeds was 49.2% which is 5% lower than that of domestic. Protein content of domestic sesame seed was 26.1% which is 14% higher than those of imported seed samples. In addition, domestic sesame seed sample contained 0.74 milligram per gram of lignan compounds which is 45% higher than those of imported seed samples. Domestic sesame showed external surface of seed coat with much more waxy stripes deposits and round shaped coat cells rather than those of imported sesame seed samples. The intersection part of the sesame seeds showed no specific difference depending on the producing origin. The experiment suggested that difference of main components such as protein, sesamin and physical structure of the sesame seed coat were useful indicator to distinguish domestic sesame seeds from imported sesame seeds.

국내산 땅콩의 일반 품종과 고올레산 품종에 대한 지용성 영양성분과 산화안정성 비교

임호정(Ho-Jeong Lim),김미소(Mi-So Kim),김다솜(Da-Som Kim),김회성(Hoe-Sung Kim),배석복(Suk-Bok Pae),김재겸(Jae Kyeom Kim),신의철(Eui-Cheol Shin) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.3

본 연구는 가열 산화에 따른 일반 및 고올레산 땅콩 유지의 화학적인 특성 변화를 측정하였다. 땅콩 유지는 용매추출법을 이용하여 추출하였으며 80oC에서 25일간 가열 산화시켜 지방산 조성, 토코페롤, 식물스테롤 및 산화안정성 변화를 측정하였다. 일반 및 고올레산 유지의 주요 지방산은 올레인산과 리놀레산으로 이들의 비율(O/L)은 일반 품종 2.75, 고올레인산 품종 5.23으로 나타났다. 저장기간동안 가열산화에 의해 리놀레산이 파괴되어 단일불포화지방산 및 포화지방산의 비율이 상대적으로 증가하였으며 일반 품종이 고올레산에 비해 리놀레산의 감소량이 유의적(p<0.05)으로 높은 것으로 나타났다. 산화안정성을 나타내는 과산화물가와 산가를 측정한 결과 두 가지 유지 모두 증가하는 것으로 일반 품종은 과산화물가와 산가가 급격히 증가하는 경향을 보였고, 고올레산 품종은 상대적으로 다소 완만하게 증가하였다. 가열산화가 진행됨에 따라 토코페롤과 식물스테롤은 모두 감소하는 경향을 보였지만, 일반품종에서 감소되는 속도가 더 빠르게 나타났다. 고올레산 품종의 경우 높은 올레인산 비율과 낮은 리놀레산의 비율로 인해 일반 품종보다 우수한 산화안정성을 보였으며, 지방산 조성뿐 만 아니라 토코페롤과 식물스테롤의 함량변화 역시 고올레산 품종에서 더 높은 잔존율을 확인할 수 있었다. Generally, peanuts are classified as high-fat foods as they possess high proportions of fatty acids. This study compared lipid constituents and properties between normal and high-oleic peanuts. Gas Chromatography-Flame Ionization Detector (GC-FID) analyses revealed that the fatty acid levels were significantly different between the normal and higholeic peanuts (p<0.05). Eight fatty acids were identified in the samples, including palmitic (C16:0), stearic (C18:0), oleic (C18:1, n9), linoleic (C18:2, n6), arachidic (C20:0), gondoic (C20:1, n9), behenic (C22:0), and lignoceric (C24:0) acids. Four tocopherol homologs were detected, and α- and γ-tocopherols were the predominant ones. Tocopherols were rapidly decomposed during 25 day storage at 80℃. The main identified phytosterols were beta-sitosterol, Δ<SUP>5</SUP>-avenasterol, campesterol, and stigmasterol. Acid and peroxide values indicated that high-oleic peanuts have better oxidative stability than normal peanuts. These results can serve as the basis for the use of peanuts in the food industry.

들깨 종간 교잡(Perilla citriodora × P. hirtella) 집단의 양적 형질 유전자좌 분석

이명희(Myoung Hee Lee),오기원(Ki Won Oh),김명식(Myung Sik Kim),김성업(Sung Up Kim),김정인(Jung In Kim),오은영(Eun Young Oh),배석복(Suk Bok Pae),여운상(Un Sang Yeo),김태호(Tae Ho Kim),이정희(Jeong Hee Lee),정찬식(Chan Sik Jung),곽도연(Do 한국육종학회 2018 한국육종학회지 Vol.50 No.1

레몬들깨(P. citriodora)와 범꼬리소엽(P. hirtella) 등 2종의 2배체 야생 들깨 종간 교잡을 통해 들깨 유전체 사업의 연관 유전지도 작성에 필요한 집단을 육성하였다. 두 종의 염색체 수는 n=10으로 같고 종간 교잡 후대는 세대를 거치는 동안 정상적인 생육과 결실을 보였다. F2 집단의 표현형은 정규분포를 보여 종간 교잡 식물체의 감수분열 과정에서 두 종의 염색체가 친화성이 높은 것으로 보였다. F2 집단으로부터 수확한 종자에서 Rosmarinic acid, Luteolin, Apigenin등 3종의 성분을 분석한 결과 4배체 재배종 들깨의 함량과 유사한 범위에 분포하였고 Apigenin과 Luteolin 간에 0.762의 높은 정의 상관이 있었다. 주요 농업형질 및 기능성분 분석 결과 21개의 QTL을 찾았다. Apigenin과 Luteolin 간에 높은 정상관이 있었으나 QTL 영역은 다르게 조사되었다. 엽색(LC)의 QTL은 3번 염색체에 하나만 존재하였는데 LOD가 14.3으으로 매우 높고 표현형 변이에 대한 QTL의 설명 비율이 50.4%로 높은 부분 우성 형질이었다. 또한 이 QTL 주변 영역에는 안토시아닌 생합성 조절인자로 알려진 basic helix-loop-helix(bHLH) DNA-binding superfamily protein, myb domain protein 43, Transducin/WD40 repeat-like superfamily protein이 확인되었다. An interspecific cross between P. citriodora and P. hirtella constitutes ideal material for a linkage map construction in genome project of Perilla. The chromosomes of the species are same with n=10 and progenies of the cross are normal in growth and seed set. The phenotype of F2 population of the cross are normally distributed and this is a proof of high affinity of the chromosomes during their sexual reproduction. Rosmarinic acid, Luteolin and Apigenin contents of F2 plants were distributed in similar range with tetraploid perilla cultivars. Luteolin and Apigenin are positively correlated with correlation coefficient of 0.762. 21 QTLs were detected in agronomic traits and Rosmarinic acid, Luteolin and Apigenin contents. Even though Luteolin and Apigenin are positively correlated, QTLs were located in different position. Purple leaf color related QTL was mapped in Chromosome 3 with LOD of 14.3, PVE of 50.4%. Three anthocyanin biosynthesis transcription factor like sequences, WD40 repeat-like superfamily protein, myb domain protein 43 and basic helix-loop-helix (bHLH) DNA-binding superfamily protein, were detected near from the QTL.