담배연기 노출량 평가 생체지표

박철훈,신한재,이형석,유지혜,손형옥,Park, Chul-Hoon,Shin, Han-Jae,Lee, Hyeong-Seok,Yoo, Ji-Hye,Sohn, Hyung-Ok 한국연초학회 2009 한국연초학회지 Vol.31 No.1

Biomarkers could be critical and useful tools for assessing the biological effects of smoking and detecting differences between potentially reduced exposure product (PREP) and conventional cigarettes. Smoking-related biomarkers can be classified into three categories as biomarkers of exposure, biomarkers of effects, and biomarkers of potential harm. When compared with the biomarkers of effects or harm, the biomarkers of exposure for chemical constituents of cigarette smoke are well established and characterized. In addition, they could offer the important information in understanding how cigarette smoke interacts with biological molecules and causes the disease to human. Therefore, we provide an overview of 6 biomarkers of exposure (Nicotine and nicotine metabolites, Carboxyhaemoglobin, NNAL (4-(methylnitrosoamino)-1-(3-pyridyl)-1-butanol) and NNAL - glucuronide, 3-Hydroxypropyl-mercapturic acid, and Monohydroxy-butenyl-mercapturic acids, and Urine mutagenicity) which were validated through extensive research and clinical experience. These reliable biomarkers could help identify the efficacy of PREP by predicting early toxicological effects and lead to improve it.

옥수수 수염의 물 추출물이 혈압에 미치는 영향

박철훈,하병국,백운상,한시준,Park, Chul-Hoon,Ha, Byoung-Kuk,Paek, Un-Sang,Hahn, See-Joon 대한약리학회 1972 대한약리학잡지 Vol.8 No.2

Zea mays Linne, which belongs to Graminae family, has been ordinarily used as edibles and fodder. Its stigma, Maydis Stigma, has been discarded without any special application with the exception of being used as diuretic in the sphere of Chinese medicine. Moreover, the pharmacological research toward Maydis Stigma as a medicinal herb has not been regretfully undertaken at all. The authors hereby paid attention to this point of view and made experiment to find out whether the water extract of Maydis Stigma might possess some pharmacological effect on the blood pressure of the rats or not. $LD_{50}$ to the mice was determined and the experiment was performed making the use of several agents related to the blood pressure such as atropine, Avil, dichloroisoproterenol and hexamethonium. The blood pressure of the rats and rabbits was recorded with the equipment of polygraph (Grass model 7 DAB) and its accessory, Statham pressure transducer. The results of the experiment are as follows. 1. $LD_{50}$ to the mice weighing 20g is determined 1,125 mg/kg. 2. The blood pressure manifests the gradual decrease by the rate of $-15.50{\pm}3.32,\;-22.14{\pm}8.73$ and $-46.25{\pm}5.17\;mmHg$ in proportion to ZW 25, 50 and 150mg/kg administered to the rats. 3. The blood pressure also manifests the same gradual decrease in proportion to ZW administered to the rabbits. 4. It is of significant difference to manifest the increase of $+5.14{\pm}4.65\;mmHg$ in the rats pretreated with atropine as compared with $-22.14{\pm}8.73\;mmHg$ of control group when administered each ZW 50mg/kg. 5. The administration of ZW 50 mg/kg to the rats pretreated with Avil, dichloroisoproterenol and hexamethonium does not manifest the significant difference in comparison with the control group.

양채송화(洋菜松花)의 알코홀 추출물이 적출 조개 심장운동에 미치는 영향

박철훈(Park, Chul-Hoon),김정일(Kim, Chung-Il) 대한약리학회 1973 대한약리학잡지 Vol.9 No.2

Lampranthus spectabilis is an ornamental plant which belongs to one of the Aizoaceae family as an one-year herb. There are no illustrations on the references that Lampranthus spectabilis has been made use for the purpose of the medicinal plant among the traditional herb doctors and the laymen. However, it has the specific characteristics to be analogous to the Cactaceae family in the botanical classification. Besides, mescaline, the main ingredient of Peyote cactus, demonstrates the sympathomimetic effect as well as the psychotomimetic effect. The authors called our attention to the resemblance to the botanical family between Aisoaceae and Cactaceae, and made a survey whether the alcohol extract of Lampranthus spectabilis might possess any pharmacological effect on the isolated heart of clam or not. The results of the experiment were as follows. 1. Administration of LX to the isolated heart of clam produced an inhibitory effect on the cardiac motility in proportion to its concentration 10^-4, 5 X 10^-4 and 10^-3 g/ml respectively. 2. Administration of LX 5 X 10^-4 and 10^-3 g/ml produced the blockade effect on the cardiac motility pretreated with serotonin 10^-7 and 10^-4 g/ml, as well as with methysergide 10^-6 and 10^-5 g/ml. 3. Administration of LX 5 X 10^-4 g/ml produced no effect on the cardiac motility pretreated with acetylcholine 10^-7 g/ml. 4. Administration of LX 5 X 10^-4 g/ml produced the blockade effect on the cardiac motility pretreated with barium chloride 10^-4 g/ml, as well as with prostaglandin 20μg. Consequently, it is concluded that the alcohol extract of Lampranthus spectabilis has an effect of depression on the motility of the isolated heart of clam. Its mechanism of action might be due to a direct action on the heart. the heart.

조기분만과 TNF-α 및 IL-1β 유전자의 다형성과의 연관성

박철훈 ( Cheol Hoon Park ),박인양 ( In Yang Park ),고현선 ( Hyun Sun Ko ),안현영 ( Hyun Young Ahn ),김수평 ( Soo Pyung Kim ),신종철 ( Jong Chul Shin ),김연희 ( Youn Hee Kim ),이귀세라 ( Gui Se Ra Lee ),이희중 ( Hee Joong Lee ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.12

목적 : 조기분만은 신생아 사망 및 이환의 가장 중요한 원인이지만 아직 원인이나 병태생리가 잘 밝혀져 있지 않다. 조기분만의 위험인자로서 자궁내 감염이 주로 거론되고 있으며, tumor necrotic factor-α(TNF-α)와 interleukin-1β(IL-1β)가 염증의 진행과정에 중요한 역할을 하는 것으로 알려졌다. 연구 방법 : 조기 진통에 의한 조산모 66명과 조기 파수에 의한 조산모 27명을 연구대상으로 하였으며 만삭에 분만한 288명을 Objective : To elucidate whether polymorphisms of tumor necrotic factor-α (TNF-α) and interleukin-1β (IL-1β) are associated with preterm delivery caused by preterm labor and preterm premature rupture of membrane (PPROM) in korean pregnant women. Methods :

담배 주류연의 생물학적 활성에 대한 흡연조건의 영향

신한재,박철훈,손형옥,이형석,유지혜,이병찬,현학철,Shin, Han-Jae,Park, Chul-Hoon,Sohn, Hyung-Ok,Lee, Hyeong-Seok,Yoo, Ji-Hye,Lee, Byeong-Chan,Hyun, Hak-Chul 한국연초학회 2008 한국연초학회지 Vol.30 No.1

The objective of this study was to determine the effect of smoking conditions on the in vitro toxicological activity of mainstream smoke. The 2R4F reference cigarette was machine-smoked by International Organization for Standardization(ISO) and Canadian Intense(CI) conditions. Smoke was analysed for chemical composition and in vitro toxicity. The cytotoxic potencies of both the total particulate matter(TPM), which were collected in Cambridge filter pad, and gas/vapor phase(GVP), which was bubbled through in phosphate-buffer saline in a gas-washing bottle, were assessed neutral red up take assay with chinese hamster ovary(CHO) cells. The assessment for genotoxicity of TPMs generated under ISO and CI conditions was determined using Salmonella mutagenicity assay and in vitro micronucleus assay. When calculated on an equal TPM basis, in vitro toxicity of TPM obtained under CI condition was decreased compared to TPM generated under ISO condition. The results of chemical composition analyses revealed that the lower toxicological activity under CI condition than that of ISO condition could be explained by the decreased in the contents of phenols, N-nitrosoamines and aromatic amines of TPM on an equal TPM basis.

담배적용 생체외(in vitro) 평가

신한재,박철훈,Shin, Han-Jae,Park, Chul-Hoon 한국연초학회 2008 한국연초학회지 Vol.30 No.1

세포독성과 유전독성 측정법은 독성 평가에 있어서 통합요소이며, 잠재적인 생물학적 활성을 측정하는 방법으로서 규제 당국에 의해 인정되는 방법이다. 이들은 빠르고 경제적인 방법이고, 오랜 사용한 역사를 가지고 있으며, 양적인 독성을 평가 할 수 있는 방법이다. 담배의 안전성을 평가하기위해 널리 이용되는 3-battery 시험법은 다음과 같다. 1. 미생물을 이용한 복귀돌연변이 시험 2. 포유류 유래 세포주를 이용한 Neutral red 세포독성 3. 포유류 유래 세포주를 이용한 소핵시험

질소 화합물이 담배연기성분 및 안전성에 미치는 영향

신한재,박철훈,손형옥,이형석,김용하,현학철,Shin, Han-Jae,Park, Chul-Hoon,Sohn, Hyung-Ok,Lee, Hyeong-Seok,Kim, Yong-Ha,Hyun, Hak-Chul 한국연초학회 2009 한국연초학회지 Vol.31 No.2

The objective of this study was to investigate the effect of nitrogen compounds such as protein on the chemical composition and toxicity of cigarette mainstream smoke. BSA protein was treated into the tobacco leaf of original 2R4F cigarette at 1~4 % level. The studies were performed which included a bacterial mutagenicity assay and a mammalian cell cytotoxicity assay for total particulate matter(TPM), and glutathione(GSH) consumption assay for gas/vapor phase(GVP) and determination of smoke chemical constitute. Cigarettes treated with protein were observed dose-dependent increase in yield of volatiles, semi-volatiles and aromatic amines compared with control cigarette. However, carbonyl compounds such as acrolein was lower than that of control cigarette when calculated on an equal TPM basis. The cytotoxicity of TPM obtained from the protein-added cigarettes was not different from that of control cigarette. However, the mutagenicity of the TPM from protein-treated cigarettes(1~4 %) was up to 10-27 % higher than that of control. On the other hand, toxicity of GVP from protein-treated cigarette(4 %) was significantly decreased compared with control cigarette. An overall assessment of our data suggests that nitrogen compounds such as protein should be important for the chemical composition and biological activity of cigarette mainstream smoke.

인간 유래 폐 세포주별 담배연기 분획의 염증 반응 민감도 비교

유지혜,손형옥,박철훈,이형석,장미,현학철,신한재,Yoo, Ji-Hye,Sohn, Hyung-Ok,Park, Chul-Hoon,Lee, Hyeong-Seok,Jang, Mi,Hyun, Hak-Chul,Shin, Han-Jae 한국연초학회 2010 한국연초학회지 Vol.32 No.1

The aim of this study is to compare the sensitivity of both two NCI-H292 and A549 cell types to acute inflammatory responses induced by cigarette smoke. For this, we treated two kinds of smoke fractions derived from 2R4F reference cigarettes: total particulate matter(TPM) collected onto a Cambridge filter pad and gas/vapor phase(GVP) prepared by bubbling through in buffer solution. When we measured cellular cytotoxicity by neutral red uptake assay after treatment for 24 hours, TPM and GVP induced cytotoxic effect in a dose-dependent manner in the range of 10-$100{\mu}g$/mL and 60-$300 {\mu}g$/mL., respectively, in both cell types without any cellular difference. Additionally, when we examined acute inflammatory responses by analyzing cytokines secreted into culture media including tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-8(IL-8), and transforming growth factor-$\alpha$(TGF-$\alpha$) as well as matrix metalloproteinase-1(MMP-1), the treatment with smoke fractions increased those marker proteins in a dose-dependent manner in NCI-H292. Meanwhile, in A549 cells only MMP-1 was observed to be increased in a dose-dependent fashion. Collectively, our data indicate that NCI-H292 cell type is more sensitive to cigarette smoke-induced inflammatory response than A549 cells. This suggests that NCI-H292 could be useful as an in vitro evaluation tool to assess harmful effects of cigarette smoke.

임신 중 난소 종양 염전에 대한 임상적 연구

최예훈 ( Ye Hoon Choi ),박철훈 ( Chul Hun Park ),이귀세라 ( Gui Se Ra Lee ),김사진 ( Sa Jin Kim ),신종철 ( Jong Chul Sin ),나종구 ( Jong Gu Na ),김수평 ( Soo Pyung Kim ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.12

목적 : 임신 중 난소 종양 염전으로 응급 수술을 시행한 군과 난소 종양으로 계획된 수술을 한 군의 특성을 비교하여 임신에 미치는 영향을 알아보고자 한다. 연구 방법 : 1990년 1월부터 2001년 12월까지 가톨릭 대학교 성모, 강남 성모, 성가 병원에서 임신중 난소 종양 염전으로 응급 수술을 받은 48예 중에서 추적 검사가 가능하였던 36예를 대상군으로, 난소 종양으로 진단되어 임신 2기초 이내에 계획된 선택적 수술을 받은 53예를 비교군으로 하여 Objective : To evaluate optimal management of an ovarian tumor in pregnancy Methods : This study includes 89 cases of an ovarian tumor in pregnancy that required surgery at Catholic Medical Center, Kangnam St. Mary`s hospital, Holy Family hospital of the

한국표준형 원전 증기발생기 Stay 용접부 자동검사시스템 및 현장 검증

임사회,박치승,박철훈,주금종,노희충,윤광식,Lim, Sa Hoe,Park, Chi Seung,Park, Chul Hoon,Joo, Keum Chong,Noh, Hee Chung,Yoon, Kwang Sik 한국압력기기공학회 2010 한국압력기기공학회 논문집 Vol.6 No.1

The stay cylinder weld at the steam generator of Korean Standard Nuclear Power Plants is safety class I component and is subjected to be inspected by the volumetric examination such as ultrasonic method. As accessibility of this area is limited due to the narrow space and high radiation, the existing manual inspection method involves various difficulties. Moreover operators may be exposed to internal contamination by contaminated dust during the surface buffing process to improve the inspection reliability of this area. Recently the new automatic inspection system for stay cylinder welds has been developed. The inspection system basically consists of a driving assembly, data acquisition device and signal processing units. The driving assembly is classified by 1) the scanner for inspecting and buffing the weld, 2) pillars for guiding the scanner and 3) the base frame for loading and supporting pillars. The scanner has 4 sensor modules to inspect in 4 refracted angles and 4 incident directions. These components can be inserted into the skirt of the stay cylinder through the manway hole and assembled easily by one-touch in the skirt. Data acquisition device and signal processing units developed in previous works are also newly upgraded for better processing of data analysis and evaluation. The system has been successfully demonstrated not only in the mock-up but also in the field. In this paper, newly developed inspection system for the stay cylinder weld of the steam generator is introduced and their field applications are discussed.