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LC / ESI - MS를 이용한 Acrylamide의 정성확인 및 정량분석
박찬구(Chan-Koo Park),조성자(Sung-Ja Jo),조남준(Nam-Joon Chough),김민영(Min-Young Kim),손종렬(Jong-Ryeul Sohn),문경환(Kyong-Whan Moon) 대한환경위생공학회 2004 대한환경위생공학회지 Vol.19 No.4
Acrylamide, difficult to analyze by GC and GC/MS due to the polarity and low volatility, was analyzed by LC/ESI/MS in the study. Acrylamide its (molecular weight 71amu) showed m/z=72 (M+H)+ and high peak intensity at 22V in SIR mode. The mass spectrum ratios of acrylamide for qualitative identification had m/z=72 in precursor ion and m/z=55 in products ion, respectively. Those ratios at 30V in SIR mode ranged from 1: 1.4 to 1:1.17 despite various acrylamide concentrations. The ion intensity ratios of acrylamide (m/z=72, [M+H]+) to acrylamide isotopes (m/z=73, [M+H]+) ranged from 100 : 3.57 to 100 : 3.92. The results verified theoretical mass spectrum ratio that was 100:3.82. The linearity of standard calibration curve was y = 520.584x + 1815.26 with r²=0.99. In quality assurance and quality control, the recovery rate ranged from 81.64 percent to 90.97 percent and relative standard deviation was less than 10% with 5 repeated injections at individual standard calibration solutions. The method was applied to analyze acrylamide in food at grocery stores. Snacks made of potatoes showed the highest acrylamide concentration followed by products made of French fries, wheat, and com.
손종렬(Jong Ryeul Sohn),문경환(Kyung Whan Moon),박찬구(Chan Koo Park) 한국환경보건학회 1998 한국환경보건학회지 Vol.24 No.4
Trichloroethylene(TCE) was the most common and abundant pollutants in the refractory substances and this was difficult to be degradable by conventional methods. Therefore, a considerable interest has been devoted to developing new process where TCE can be easily decomposed. In this study, the series of ultrasonic irradiation for removal of TCE in aqueous solution has been selected as a model reaction in the batch reactor system in order to obtain the basic data investigate the influence of various experimental parameters such as pH, reaction temperature, and intensity. The products obtained from the ultrasonic irradiation were analysed by GC/MS and UV spectroscopy. The intermediates of ultrasonic irradiation of TCE was identified as Cl₂, HCl, Cl⁻, chloroform, dichloroethene, trichloroethane , and tetrachloroethane. After the ultrasonic irradiation of TCE con - taining aqueous solution for 20 min their pH s were decreased from 7.0 to 2.4 respectively. Further irradiation of this solution did not change their pH values. This can be attributed to the formation of HCl obtained by the reaction between H · radical and Cl⁻ which could be produced by the ultrasonic irradiation of water and reactants containing chlorine. Acidic solution showed a good capability in the degradation of TCE. In general, the rate of reaction is proportional to the reaction temperature obeying the Arrhenius law. However, in the ultrasonic irradiation, this suggests as the reaction temperature increase the decomposition rate of reactant decrease. This result meant that the increase of reaction temperature due to the increase of vapor pressure of water accelerated the decrease of acoustic intensity which was can be proportional to the decomposition rate of TCE solution. It was found that more than 80% of TCE solution was removed within 2 hours in all reaction conditions. The reaction order in the degradation of TCE solution was verified as the pseudo-first order. From the fore-mentioned results, it can be concluded that the refractory organic compounds could be removed by the ultrasonic irradiation with radicals, such as H · and OH · radical causing the high increase of pressure and temperature. Finally, it appeared that the technology using ultrasonic irradiation can be applied to the treatment of refractory substances which are difficult to be decomposed by the conventional methods.
급성 알콜 투여 흰쥐에서 녹두 함유 복합생약제제의 간 중성지방 축적억제 및 알콜대사 촉진효과
김문희(Moon Hee Kim),권오협(Oh Hyep Kwon),박찬구(Chan Koo Park) 대한약학회 1996 약학회지 Vol.40 No.1
An ethanol administration causes hepatic triglyceride accumulation in rats. To assess whether the herbal extract containing Phaseoli radiati semen(herbal extract) inhibits the triglyceride accumulation in the liver, we determined the hepatic triglyceiide levels in rats fed ethanol and the herbal extract. In addition, the blood ethanol concentrations and the activities of hepatic alcohol dehydrogenase(ADH) and aldehyde dehydrogenase(ALDH) were measured to determine the effects of the herbal extract on alcohol metabolism in rats. The administration of the herbal extract markedly reduced the triglyceride levels elevated by ethanol in the liver as well as in the serum. The herbal extract remarkably lowered blood ethanol concentrations in a dose-dependent manner. The ADH activities decreased by ethanol were recovered to the normal level by the herbal extract treatment. Moreover, the ALDH activities slightly decreased by ethanol increased beyond the normal level by the herbal extract treatment. We conclude that the herbal extract inhibits the hepatic triglyceride accumulation and stimulates alcohol metabolism by preventing ADH and ALDH from inhbition by the ethanol administration in the rat liver.