Micronucleus Test of Pinella Rhizoma Aqueous Extracts in Bone Marrow Cells of Male ICR Mice - In Vivo Genotoxicity

박지하(Jiha Park) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.3

In this research, the genotoxic effects of Pinelliae Rhizoma (PR) extracts, one of famous herbal agents in Korean medicine were evaluated using the mouse micronucleus test. PR extracts was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000, and 500 ㎎/㎏. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. No PR extracts treatment-related abnormal clinical signs, body weight changes and mortalities were detected. Significant (p<0.01) increases of the numbers of polychromatic erythrocytes contain micronucleus in prepared bone marrow cells were detected in CPA and PR extracts 2000 ㎎/㎏ treated groups as compared with intact control, respectively. The results of intraperitoneal dose mouse bone marrow cell micronucleus test of PR extracts were positive in the present study. It is considered that there were no problems from cytotoxicity of PR extracts tested in this study because the polychromatic erythrocyte ratio was detected as > 0.42 in all tested groups.

安神藥의 毒性에 관한 문헌적 고찰

박지하(Jiha Park),이상남(Sangnam Lee),노성수(Seongsoo Roh),김용현(Yonghyun Kim),서부일(Buil Seo) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.1

Objectives & Method : We investigated toxicity, poisoning symptoms, poisoning treatment and prevention against poisoning of mind-tranquilizing herbal medicines(安神藥) in order to use herbal medicines accurately. Result : Cinnabaris(朱砂), Zizyphi Spinosae Semen(酸棗仁), Polygalae Radix(遠志), Ganoderma(靈芝) and Polygoni Multiflori Ramulus(夜交藤) may give rise to some side effects or toxic symptoms in mind-tranquilizing herbal medicines(安神藥). The representative methods of poisoning treatment in western medicines are washing out the stomach, promotion of vomiting, causing diarrhea, supplies of grape sugar and symptomatic treatment, etc. The representative methods of poisoning treatment in oriental medicine take advantage of herbs. And Oriental medical doctor should meet symptoms as patients call for attention. In order to prevent against poisoning of mind-tranquilizing herbal medicines(安神藥), the patients should keep usage, dosage and notes and oriental medical doctors should do processing drugs. Conclusion : We should pay attention to clinical using of Cinnabaris(朱砂), Zizyphi Spinosae Semen(酸棗仁), Polygalae Radix(遠志), Ganoderma(靈芝) and Polygoni Multiflori Ramulus(夜交藤) in mind-tranquilizing herbal medicines(安神藥).

東樞鍼의 비침습적 神門穴 자극이 심박변이도에 미치는 영향

박황진(Hwangjin Park),이상남(Sangnam Lee),박지하(Jiha Park),한창현(Changhyun Han) 한국한의학연구원 2011 한국한의학연구원논문집 Vol.17 No.1

‘Dong Chu Chim(DCC)’ is invented by ‘Dong-Chu Han Medicine Institute(東樞桓醫學硏究院)’ and Oriental medicine doctors at ‘Dong-Chu Han Medicine Institute’ apply DCC clinically for Qi-gong therapy. Since the DCC is shaped similar to ‘round-point acupuncture(圓鍼)’ and the tip of DCC is round, it can stimulate the acupoints of skin non-invasively, unlike filiform acupuncture(毫鍼). The present study was performed to verify the clinical usefulness of DCC by measuring heart rate variability(HRV) that reflect change of the autonomic nervous system. Twenty-five healthy subject were treated with DCC or filiform acupuncture(毫鍼) in turn and HRV was measured during five minutes for three times (before, during, and after treatment, called as stage Ⅰ,Ⅱ,Ⅲ respectively). As compared with stage Ⅰ, mean heart rate(HRT) was decreased and standard deviation of all normal R-R intervals (SDNN) was increased significantly in stage Ⅱ and Ⅲ of treatment of two types of acupunctures. Log-transformed total power(LOGTP) and log-transformed very low frequency power(LOGVLF) were increased significantly in stage Ⅲ compared with stage Ⅰ, especially subjects treated with DCC displayed significant increase of LOGVLF and LOGTP in stage Ⅱ compared with stage Ⅲ. Additionally, there were no significant differences between both type of acupunctures in terms of its effect on HRV of each stage. From these findings, It seems that non-invasive stimulus of DCC affects on the autonomic nervous system and can control the balance of that like filiform acupuncture. Therefore, it would be expected that the doctor can apply DCC clinically for patients who have needle phopia or children. It is suggested that additional studies about the effect of DCC on other acupoints and comparison study about the effect of DCC with those of the finger-pressure treatment using other tool should be done in the future.

모형실험을 이용한 벼재배 과정에서 온실가스 발생특성 고찰

최영준 ( Yeongjun Choi ),박지하 ( Jiha Park ),하수민 ( Sumin Ha ),임건휘 ( Geonhwi Lim ),유찬 ( Chan Yu ) 한국농공학회 2022 한국농공학회 학술대회초록집 Vol.2022 No.-

최근 지구온난화로 인해 지구의 연평균 기온이 상승하고 있는데, 이에 원인으로 지목되고 있는 주요 온실가스에는 이산화탄소(CO2), 메탄(CH4) 등이 있다. 이와 같은 온실가스 중에서도 특히 장기체류 온실가스(Long-lived greenhouse gases, LLGHGs)인 메탄(CH4)은 이산화탄소(CO2) 보다 약 15~34배 강하게 온난화에 영향을 주는 기체이다. 1800년대 산업화 이후부터 대기 중 메탄가스 농도는 꾸준한 증가하는 추세를 보이며, 산업화 이전 722 ppb 대비해서 2020년 1,879ppb로 260% 증가하는 결과도 발표된 바 있어 CH4의 증가량은 다른 온실가스에 비해 매우 높은 수치를 나타내고 있다(Butler and Montzka, 2020; WMO, 2020). 그런데 CH4의 발생은 산업화 외에도 농업과 축산분야에서 미치는 영향이 큰데, 특히 논에서 연간 약 60Tg의 CH4가 배출되어 지구 전체 CH4 배출량의 5~30%를 차지하는 것으로 알려져 있다(Ko et al., 1998). 우리나라도 논에서 벼를 재배하는 나라로서 벼를 재배하는 기간에 발생하는 CH4의 발생현황과 감축과 관련된 대책을 고민할 필요가 있다. 본 발표에서는 아크릴로 토조를 제작하여 LED 조명으로 일사량을 조절하고, PVC필름으로 커튼을 설치하여 내부 공기의 순환을 조절할 수 있는 챔버(chamber)를 제작하여, 실제로 벼를 재배하면서 온실가스, 특히 메탄의 발생특성을 측정하여 그 결과를 고찰하였다. 벼의 생장으로 인한 가스 변화와 Chamber 내의 환경을 파악하기 위해 가스 측정기(CH₄, CO₂, O₂), 조도계, Eh-측정기, pH meter, 온ㆍ습도계를 이용하여 측정하였고, HFP (Heat Flux Per square meter), 수분장력계(Tensiometer), 토양온도계, 체적함수비(Volumetric Water Content)를 측정하였다. 온실가스 농도, 조도, pH, Eh는 일일 2회 측정하였으며, 다른 자료는 자동수집 장치를 설치하여 24시간 기록하여 분석하였다. 또한 주 1회 벼의 생장길이를 측정하였다.

아유르베다와 티베트 의학의 기본이론과 한의학과의 비교 고찰

한창현(Changhyun Han),박지하(Jiha Park),이상남(Sangnam Lee),송익수(Icksoo Song),이봉효(Bonghyo Lee) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.3

Background : There has been increased global interest in traditional medicine. Ayurveda and Korean medicines are most world-widely spread as Complementary and Alternative Medicine(CAM). Objectives : This review gives an overview of basic principles and commonalities of Ayurveda, Tibetan and Korean medicine. Method : I have summarized the results of the research into the following categories: basic theoies, history, physiology and pathogenesis. Result : 1. Ayurveda is the medical science that grasps individual constitution through Tridosha, a combination of five primary elements(space, wind, fire, water, and earth), and systematically explains physiological and pathological phenomena which reveal according to the constitution. 2. The theoretical basis forming the Tibetan Medicine is the substance of phlegm, bile, and wind each of which is divided into five kinds. These physiological substances can be transformed into pathological factors when certain environment is formed. 3. Ayurveda, Tibetan and Korean medicine, three main axes of the traditional medicine arouse western medical researchers' interest by their peculiar views of the disease and the system of their medical theories. And they are expected to render services to human health. Conclusions : We can expect the potential of the approaching between Ayurveda, Tibetan and Korean medicine.

女貞子와 ?寄生이 난소적출로 유발된 흰쥐의 골다공증 치료효과에 미치는 영향

이상윤(Sangyun Lee),서부일(Buil Seo),박지하(Jiha Park),노성수(Seongsoo Roh),김대준(Daejun Kim) 한국한의학연구원 2011 한국한의학연구원논문집 Vol.17 No.1

Objectives : The present study had been undertaken to investigate the effects of Ligustri Lucidi Fructus Extract and Vici Herba Extract on treatment of osteoporosis in ovariectomized rats. Method : In this experiment, the rats were ovariectomized. Rats were individually administered by water extract from Ligustri Lucidi Fructus or Vici Herba(dose:1㎖/100g, 2times/day). The levels of bone mineral density, serum components, urinary components, bony components, body weight and uterus weight were measured. Results : The levels of spinal and femoral bone mineral density(BMD) were significantly increased in comparison with OVX group at 8 weeks after administration in Vici Herba group. The levels of serum osteocalcin showed significant decrease in comparison with OVX group at 4 weeks after administration in Ligustri Lucidi Fructus and Vici Herba group. The levels of serum ALP and phosphorus showed significant decrease in comparison with OVX group at 4, 8 weeks in after administration in Ligustri Lucidi Fructus and Vici Herba group. The levels of urinary deoxypyridinoline showed significant decrease in comparison with OVX group in Ligustri Lucidi Fructus group. The levels of fibula-tibial ash weight showed significant increase in comparison with OVX group after administration in Ligustri Lucidi Fructus group. The levels of body weight were significantly decreased in comparison with OVX group at 4 weeks after administration in Ligustri Lucidi Fructus group and at 4, 8 weeks after administration in Vici Herba group. The levels of uterus weight were not significantly increased in comparison with OVX group in Ligustri Lucidi Fructus and Vici Herba group. Conclusion : Reviewing these experimetal results, it appeared that Ligustri Lucidi Fructus and Vici Herba had efficacy on treatment of osteoporosis.

한의과대학에서의 기공교육 향상을 위한 강의 만족도 조사

김기진(Kijin Kim),한창현(Changhyun Han),박지하(Jiha Park),최성훈(Seonghun Choi),이상남(Sangnam Lee) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.1

Objectives : To understand the basis for future improvement and development in the lectures, an evaluation was made on the lecture by the 'theory-practice-self study' system initiated in 'Yangseng Qigong(養生氣功) and practice' at Daegu Haani University. Method : We surveyed the satisfaction of 'Yangsaeng Qigon and Practice' which is the lecture that is composed theory, practice, self study for 120 students at college of oriental medicine in Daegu Haani University. Result : In the inquiry for the satisfaction with the general class of ‘Yangsaeng Qigong and practice', 69% of students answered positively. As to the satisfaction with class grouping, 76% answered affirmatively, and as to the question "Agreement to the plan workable?", 72% said it was satisfactory, showing lectures by class grouping were made successful as planed. As to satisfaction with theory lectures, 36% were satisfied (38% unsatisfied), as to practice 90% was satisfied (2% unsatisfied) and as to the self-study and special lectures, 36% was satisfied (23% unsatisfied), showing the satisfaction was relatively higher for practice. To the question "Was it helpful in understanding hygiene Danjeon-breathing science?", 31% said yes (16% no) for theory, 87% yes (3% no) for practices, 32% yes (32% no) for special lectures, and 37% yes (28% no) for team assignments. Conclusions : The textbooks and majors of the lecturers turned out to be varied, but as seen from the result of lecture satisfaction on 'Yangseng Qigong and practice', it was different according to the preparedness of the lecturers and satisfaction with the lecture material. Despite the situation that most schools have chosen it as a major subject, the subject name was divergent among schools and the textbook and the content of practice were also not unified. At the point of globally rising interest in Qigong, this will only lead to its weak competitiveness when advancing to the world.

논에서 온실가스 발생 측정을 위한 모형실험

나서영 ( Seoyeong Na ),최영준 ( Yeongjun Choi ),김다빈 ( Dabin Kim ),박지하 ( Jiha Park ),하수민 ( Sumin Ha ),유찬 ( Chan Yu ) 한국농공학회 2021 한국농공학회 학술대회초록집 Vol.2021 No.-

氣功 姿勢가 뇌파에 미치는 영향

정대선(Daesun Jung),한창현(Changhyun Han),박수진(Soojin Park),이상남(Sangnam Lee),박지하(Jiha Park) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.1

This study aimed to investigate the effect of four common types of Qigong position (standing, sitting, supine, and horse-riding position) on the autonomic nervous system. Thirty healthy subjects participated in this study once a week for four weeks. Electroencephalogram (EEG) was measured three times (before, during, and after the position) while the subject maintained one of four positions for ten minutes. There were significant changes in HRV components compared with EEG power spectra in the standing position. Especially, the ratio of low-to-high frequency (LF/HF) which represents a state of balance of autonomic nervous system was increased. In the sitting position, β wave which reflects a state of alert consciousness was increased and both the sympathetic and parasympathetic nerves were activated. On the other hand, in the spine position, θ wave which signifies a state of relaxation was increased and heart rate (HR) was decreased. Activation of sympathetic and parasympathetic nerves was also observed in this position. Significant increases of indices related to awakening and concentration were observed accompanied by increase of HR and a sympathetic nerve was activated in the riding-horse position. In the present study, it was shown that each Qigong position caused various and significant changes in autonomic nervous system. It would be expected that these results can be applied in the choice of appropriate Qigong position according to objective of Qigong therapy although it is remained to further evaluate the effects of long-term maintenance of Qigong positions and repeated Qigong training.

古典醫書에 나타난 導引氣功 方法에 대한 硏究

김현태(Hyuntai Kim),한창현(Changhyun Han),이상남(Sangnam Lee),박지하(Jiha Park) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.2

Objectives : Because of emphasizing a side of preventive medicine in the oriental medicine, an interest in Doinqigong (導引氣功: Physical and breathing exercise) has been elated recently. But, it has a limited sphere of application in the present south korea. Therefore we would like to bring out its sphere of application and detailed method in the oriental medicine classics. Method : We have researched theories and methods of Doinqigong in the Junghwaeujeon(中華醫典: Oriental medicine classic collections) DB according to below the procedure. (1) Making a related words list: We have used existing study of Doinqigong to make a list. It has been connected with Doinqigong. It includes not only technical terms, but also general terms. (2) Searching sentences: We have searched sentence that contain terms related with Doinqigong in the Junghwaeujeon DB. (3) Analysis of related sentences: We have searched and classified sentence by theory and method. Result : Results & Conclusion : (1) The total number of oriental medicine classics connected with Doinqigong is twelve. (2) The methods of Doinqigong have classified into three large groups: They are the Doinqigong for preservationing of good health, the Doinqigong for curing disease and the Doinqigong for internal organs and channels. (3) The number of Doinqigong for preservationing of good health in the seven oriental medicine classics is about ten. For example, the Okeumhi(五禽戱: Five animal's dance), the Paldangeum(八段錦: Eight motions of qigong) and so on. The number of oriental medicine classics connected with Doinqigong for curing disease is seven. They contain internal diseases, surgical diseases, diseases of five sensory organs and various diseases in the order of their frequency. The Doinqigong for internal organs and channels is only the Yookjageul(六字訣: Six sound's treatment).