Regulation of Luteinizing Hormone Release and Subunit mRNA by GnRH and Ovarian Steroids in Cultured Anterior Pituitary Cells

김창미,박일선,유경자,Kim, Chang-Mee,Park, Il-Sun,Ryu, Kyung-Za The Korean Society of Pharmacology 1994 대한약리학잡지 Vol.30 No.1

흰쥐의 뇌하수체 전엽배양세포에 gonadotropin-releasing hormone (GnRH)을 처리하였을 때 시간이 경과함에 따라 GnRH농도에 비례하여 luteinizing hormone(LH)의 분비가 증가하였으며, 2시간까지 급격하게 증가하였다. 또한 GnRH를 처리하였을때 ${\alpha}$ subunit mRNA의 농도는 증가하지 않았으나 $LH{\beta}$ subunit mRNA의 농도는 GnRH 농도에 비례하여 증가하였으며, GnRH 처리후 6시간 이후부터 유의하게 증가하였다. 특히 최종농도가 $2{\times}10^{-10}M$이 되도록 GnRH를 처리하였을 때 $LH{\beta}$ subunit mRNA 농도가 2.7배 정도 최대로 증가하였다. 또한 estradiol을 단독으로 또는 GnRH와 동시에 처리하였을때 LH분비가 증가하지 않았으나 progesterone을 GnRH와 동시에 처리하였을때 LH분비가 유의하게 증가하였다. 또한 $LH{\beta}$ subunit mRNA의 농도는 estradiol및 progesterone을 단독으로 또는 GnRH와 동시에 처리하였을때 난소호르몬 농도에 의존적으로 $LH{\beta}$ subunit mRNA의 농도가 증가하였다. Estradiol에 의한 $LH{\beta}$ subunit mRNA의 증가양상은 estrogen 길항제인 LY117018에 의하여 유의하게 감소하였다. 이러한 결과로 보아 GnRH는 steady state $LH{\beta}$ subunit mRNA 농도에 영향을 미치므로써 LH분비 및 LH subunit 생합성을 조절하며 난소호르몬은 뇌하수체에 직접 작용하여 LH분비 및 LH subunit 생합성에 영향을 주는 것으로 보인다. The effects of gonadoropin-releasing hormone (GnRH) and ovarian steroid hormones on the release of luteinizing hormone (LH) and its subunit mRNA levels were investigated in anterior pituitary cells in culture. LH concentration was measured by a specific radioimmunoassay and mRNA levels of u and $LH{\beta}$ subunits by RNA slot blot hybridization assay. GnRH stimulated LH release in a dose-dependent manner from cultured pituitary cells. However, the basal LH release in the absence of GnRH was not changed during the course of 24h culture, strongly suggesting that release of LH is directly controlled by GnRH. The treatment of the pituitary cells with GnRH increased $LH{\beta}$ subunit mRNA levels in a dose-dependent manner, reaching the maximum with $2\;{\times}\;10^{-10}M$ GnRH while no significant increase in ${\alpha}$ subunit mRNA levels was observed after GnRH treatment. Estradiol did not augment GnRH-induced LH release while progesterone augmented GnRH-induced LH release in a dose-dependent manner at the level of pituitary. However, estradiol and progesterone increased basal and GnRH-induced $LH{\beta}$ subunit mRNA levels in a dose-dependent manner. The treatment of estrogen antagonist, LYI17018 blocked the effect of estradiol on GnRH-induced $LH{\beta}$ subunit mRNA levels in a dose-dependent manner while progesterone antagonist, Ru486 tended to block the effect of progesterone on GnRH-induced $LH{\beta}$ subunit mRNA levels. It is therefore suggested that GnRH Playa a major role in LH release and subunit biosynthesis by influencing the steady state $LH{\beta}$ subunit mRNA loves and ovarian steroid hormones modulate subunit biosynthesis via directly acting on pituitary gonadotropes.

공동주택의 중량충격원에 의한 진동해석 연구

윤진호(Yun, Jin Ho),박일선(Park, Il Sun),노삼영(Noh, Sam-Young) 한국콘크리트학회 2005 한국콘크리트학회 학술대회 논문집 Vol.2005 No.5

흰쥐 뇌하수체전엽 배양세포에서 GnRH 및 난소호르몬에 의한 LHβ subunit 유전자 발현 조절에 관한 연구

김창미(Chang-mee Kim),박일선(Il Sun Park),유경자(Kyung-za Ryu) 대한약리학회 1994 대한약리학잡지 Vol.30 No.1

흰쥐의 뇌하수체 전엽배양세포에 gonadotropin-releasing hormone (GnRH)을 처리하였을 때 시간이 경과함에 따라 GnRH농도에 비례하여 luteinizing hormone(LH)의 분비가 증가하였으며, 2시간까지 급격하게 증가하였다. 또한 GnRH를 처리하였을때 α subunit mRNA의 농도는 증가하지 않았으나 LHβ subunit mRNA의 농도는 GnRH 농도에 비례하여 증가하였으며, GnRH 처리후 6시간 이후부터 유의하게 증가하였다. 특히 최종농도가 2 × 10^-10M이 되도록 GnRH를 처리하였을 때 LHβ subunit mRNA 농도가 2.7배 정도 최대로 증가하였다. 또한 estradiol을 단독으로 또는 GnRH와 동시에 처리하였을때 LH분비가 증가하지 않았으나 progesterone을 GnRH와 동시에 처리하였을때 LH분비가 유의하게 증가하였다. 또한 LHβ subunit mRNA의 농도는 estradiol및 progesterone을 단독으로 또는 GnRH와 동시에 처리하였을때 난소호르몬 농도에 의존적으로 LHβ subunit mRNA의 농도가 증가하였다. Estradiol에 의한 LHβ subunit mRNA의 증가양상은 estrogen 길항제인 LY117018에 의하여 유의하게 감소하였다. 이러한 결과로 보아 GnRH는 steady state LHβ subunit mRNA 농도에 영향을 미치므로써 LH분비 및 LH subunit 생합성을 조절하며 난소호르몬은 뇌하수체에 직접 작용하여 LH분비 및 LH subunit 생합성에 영향을 주는 것으로 보인다. The effects of gonadoropin-releasing hormone (GnRH) and ovarian steroid hormones on the release of luteinizing hormone (LH) and its subunit mRNA levels were investigated in anterior pituitary cells in culture. LH concentration was measured by a specific radioimmunoassay and mRNA levels of u and LHβ subunits by RNA slot blot hybridization assay. GnRH stimulated LH release in a dose-dependent manner from cultured pituitary cells. However, the basal LH release in the absence of GnRH was not changed during the course of 24h culture, strongly suggesting that release of LH is directly controlled by GnRH. The treatment of the pituitary cells with GnRH increased LHβ subunit mRNA levels in a dose-dependent manner, reaching the maximum with 2 × 10^-10M GnRH while no significant increase in α subunit mRNA levels was observed after GnRH treatment. Estradiol did not augment GnRH-induced LH release while progesterone augmented GnRH-induced LH release in a dose-dependent manner at the level of pituitary. However, estradiol and progesterone increased basal and GnRH-induced LHβ subunit mRNA levels in a dose-dependent manner. The treatment of estrogen antagonist, LYI17018 blocked the effect of estradiol on GnRH-induced LHβ subunit mRNA levels in a dose-dependent manner while progesterone antagonist, Ru486 tended to block the effect of progesterone on GnRH-induced LHβ subunit mRNA levels. It is therefore suggested that GnRH Playa a major role in LH release and subunit biosynthesis by influencing the steady state LHβ subunit mRNA loves and ovarian steroid hormones modulate subunit biosynthesis via directly acting on pituitary gonadotropes.

중량충격원에 의한 라멘구조 공동주택의 동적특성에 관한 연구

노삼영 ( Noh Sam-young ),박일선 ( Park Il-sun ) 한국구조물진단유지관리공학회 2005 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.9 No.2

Due to the development of design and material technology in the architectural engineering, apartment buildings constructed with the wall type structure have been more spacious and the span of the structure will be longer. However, the structure with long span has generally inefficient damping effect of the floor heavy impact noise. For the present, the solution of this problem in the wall type structure could not be suggest. One of the effective solution for the problem could be the construction of apartment building with the rahmen structure. This study will show vibration properties of rahmen structures, which dominates the noise transfer in the structure, through comparing experiment results with ones of numerical analysis.

중량충격원에 의한 라멘구조 공동주택의 동적특성에 관한 연구

노삼영(Noh Sam-Young),박일선(Park Il-sun) 한국구조물진단유지관리학회 2005 한국구조물진단학회 학술발표회논문집 Vol.9 No.2

Due to the development of design and material technology in the architectural engineering, apartment buildings constructed with the wall type structure have been more spacious and the span of the structure will be longer. However, the structure with long span has generally inefficient damping effect of the floor heavy impact noise. For the present, the solution of this problem in the wall type structure could not be suggest. One of the effective solution for the problem could be the construction of apartment building with the rahmen structure. This study will show vibration properties of rahmen structures, which dominates the noise transfer in the structure, through comparing experiment results with ones of numerical analysis.

성취목표지향성 프로그램의 개발과 효과검증

박병기(Byung Gee Bak),박일선(Il Sun Park),고유림(Yu Lim Go) 한국교육심리학회 2009 敎育心理硏究 Vol.23 No.3

韓·日關係를 바라보며

박일선 건국대학교 1996 政治論叢 Vol.31 No.-

기관지폐염에 이환된 영아의 간호

박일선 이화여자대학교 간호과학대학 1970 이화간호학회지 Vol.- No.4

Regulation of Gonadotropin Release and Subunit mRNA Levels by GnHR and Ovarian Steroids in Cultured Anterior Pituitary Cells

Park, Il Sun,Kim, Changmee,Ryu, Kyungza 대한내분비학회 1990 Endocrinology and metabolism Vol.5 No.부록2호

색소건피증(XP)세포에 있어 절제회복 기작과 염색체 인자의 연관성에 관한 연구

이형호,박상대,박종군,이명애,박일선 한국유전학회 1986 Genes & Genomics Vol.8 No.2

Unscheduled DNA synthesis in XP1EH (complementation group A) cells exposed to UV-light (10J/㎡), permeabilized and repairlabeled with ^3H-dTTP was remarkably increased by the treatment with cell extracts of HeLa or XP3KA (complementation group C) cells. Single strand breaks of XP group A or C cells, which were UV-irradiated and fused with HeLa cells, produced a similar extent of those in HeLa cells irradiated with the same dose of UV-light. As a possible involvement of chromosomal factor related to the deficiency of XP cells, the activity of DNA topoisomerase II was assayed. Novobiocin, an inhibitor of DNA topoisomerase II, greatly reduced DNA single strand breaks induced by UV-light in a dose dependent manner in normal HeLa, GM 38 and CHO-K1 cells, and the activity of topoisomerase II was increased to 1.4 fold of unirradiated control at 4hrs after UV-irradiation. These results indicate that topoisomerase II participates in the repair of UV-induced DNA damage and that cellular topoisomerase activity is increased in response to structural alterations of DNA. When normal cells were exposed to low doses of UV-light (pretreatment), chased for the time periods showing the maximal induction of topoisomerase activity and exposed to high doses of UV-light or 4NQO, the survival or DNA synthesis was significantly increased compared to those with no UV-pretreatment. The apparent failure of these inducible repair in XP1EH cells was shown and discussed.