위선암종의 예후인자로서 p53, CD44v6과 VEGF 단백 발현

박언섭,이창영,이태진,김미경,유재형,Park, Eon-Sub,Lee, Chang-Young,Lee, Tae-Jin,Kim, Mi-Kyung,Yoo, Jae-Hyung 대한위암학회 2001 대한위암학회지 Vol.1 No.1

Purpose: The p53 protein is a tumor supressor gene, and its mutation is associated with biologic aggressiveness. CD44v6, one of the CD44 family, is a cell surface glycoprotein that plays a role in cancer invasion and metastasis. Vascular endothelial growth factor (VEGF) is another recently identified growth factor with significant angiogenic properties. The purpose of this study was to investigate p53, CD44v6, and VEGF expressions to determine whether degree of expression was related to pathological parameters such as Lauren's classification, depth of invasion, and lymph node metastasis. Materials and Methods: Immunohistochemical stains of p53, CD44v6, and VEGF in formalin-fixed paraffin-embedded tissue sections of 125 gastric adenocarcinomas were done. Results: The overall expression rates of p53, CD44v6, and VEGF were $54.4\%$ (68/125), $36.8\%$ (46/125), and $48.0\%$ (60/125), respectively. The p53, not CD44v6 and VEGF was higher in intestinal-type gastric carcinomas by Lauren's classification. The expressions of p53, CD44v6, and VEGF were statistically correlated with depth of tumor invasion. The expression of CD44v6 was higher in the lymph node metastatic group than in the negative group. The p53 expression was significantly associated with VEGF expression. Conclusions: These data suggest that the expressions of p53, CD44v6, and VEGF are biologically related to malignancy. The p53 and CD44v6 expressions are independent; however, p53 gene mutation is one of the contributing factors to VEGF expression in gastric adenocarcinoma.

랏트의 기관내 Fe 노출후 Fe 이동에 대한 연구

권민,최병선,박언섭,정남현,박성조,임영,박정덕,Kwon, Min,Choi, Byung-Sun,Park, Eon-Sub,Chung, Nam-Hyun,Park, Sung-Jo,Lim, Young,Park, Jung-Duck 대한예방의학회 2004 예방의학회지 Vol.37 No.4

Objectives : Iron (Fe) is an essential element in biological processes; however excessive Fe is harmful to human health. Some air pollutants contain a high level of Fe, and the human lung could therefore be over-exposed to Fe through inhaled air pollutants. This study was performed to investigate the role of metal transporters (divalent metal transporter 1, DMT1, and metal transporter protein 1, MTP1) in the lung under the environments of Fe deficiency in the body and Fe over-exposure in the lung. Methods : Rats were fed Fe deficient (FeD, 2-6 mg Fe/kg) or Fe supplemented (FeS, 120 mg Fe/kg) diet for 4 weeks, followed by a single intratracheal instillation of ferrous sulfate at low (10 mg/kg) or high (20 mg/kg) dose. Fe concentration was analyzed in the serum, lung and liver, and histopathological findings were observed in the lung at 24 hours after Fe administration. The level of DMT1 and MTP1 expression in the lung was analyzed by RT-PCR. Also, the effect of Fe deficiency in the body was evaluated on the level of Fe concentration and metal transporters compared to FeS-diet fed rats at the end of 4-week FeD or FeS diet. Results : The 4-week FeD diet in rats induced an Fe deficiency anemia with decreased serum total Fe, increased unsaturated Fe binding capacity and hypochromic microcytic red blood cells. The concentration of Fe in the lung and liver was lower in the FeD-diet fed rats than in the FeS-diet fed rats. The level of metal transporters mRNA expression was higher in the FeD-diet fed rats than in the FeS-diet. The concentration of Fe in the lung was increased in a dose-dependent pattern after intratracheal instillation of Fe into the rats, while the level of Fe in the serum and liver was not increased in the low-dose Fe administered rats. Therefore, DMT1 and MTP1 mRNA was highly expressed in both FeD-diet and FeS-diet fed rats, after intratracheal instillation of Fe. Conclusions : DMT1 and MTP1 mRNA were more highly expressed in FeD-diet fed rats than in FeS-diet fed rats. The over-exposure of Fe intratracheally induced high expression of metal transporters and increased Fe deposition in the lung in both FeD-diet and FeS-diet fed rats, but did not increase the Fe level of the serum and liver in low-dose Fe administered rats. These results suggest that the role of metal transporters in the lung might be different in a part from the duodenum under the environment of over-exposure to Fe.

외이도에 발생한 다형선종 1례

이현진(Hyun Jin Lee),박언섭(Eon Sub Park),문인석(In Seok Moon),양훈식(Hoon-Shik Yang) 대한두개저학회 2008 대한두개저학회지 Vol.3 No.2

Primary neoplasms of the external ear canal are rare, and 5% of these tumors are of glandular origin. Ceruminal glands are modified sweat glands, confined to the skin lining of the cartilaginous part of the external auditory canal. Those may give rise to tumors such as ceruminous adenoma, pleomorphic adenoma, ceruminous adenocarcinoma, adenoid cystic carcinoma, and mucoepidermoid carcinoma. An alternative theory holds that pleomorphic adenomas may well arise from ectopic salivary tissue present in the external ear canal. These tumors are very rare and usually have a benign course but there are some controversies on the therapeutic principle of pleomorphic adenoma in external ear canal. A case of pleomorphic adenoma of ceruminous glands is presented along with literature review.

사람 태아 고환에서 간질세포 분화의 형태학적 관찰

이계일,김대중,김경용,박언섭,Rhee, Kye-Il,Kim, Dae-Joong,Kim, Kyung-Yong,Park, Eon-Sub 한국현미경학회 1999 Applied microscopy Vol.29 No.4

본 연구는 여러 가지 원인으로 인공 유산된 사람 태아 17예를 태령 14주에서 27주로 분류한 뒤, 고환간질내에서 간질세포의 미세구조를 광학현미경과 전자현미경을 이용하여 관찰한 결과 다음과 같은 결론을 얻었다. 1. 사람 태아 고환의 간질세포를 형태적으로 방추형간질세포, 밝은 간질세포, 어두운 간질세포 그리고 변성 간질세포로 분류할 수 있었다. 2. 미성숙한 방추형간질세포는 14주에 많이 관찰되었으며 점차 분화되어 밝은 간질세포와 어두운 간질세포로 변하였다. 3. 밝은 간질세포와 어두운 간질세포는 17주와 24주에 가장 많이 분포하였으며 변성 간질세포는 27주에 많이 관찰되었다. 4. 무과립세포질세망은 밝은 간질세포내에서 현저히 관찰되었으며 점차 내강이 팽대되어 세포질 전반을 차지하고 있었다. 5. 사립체는 밝은 간질세포에 많이 분포하였고 사립체능은 농축되어 진하게 나타났으며, 어두운 간질세포에서 전자밀도가 높은 사립체 함유물을 포함하고 있었다. 6. 지방소적은 태령 14주부터 관찰되어 16주에 가장많은 분포를 보였고 20주 이후 감소하는 양상이 관찰되었다. 7. 간질세포내에 풍부한 양의 당원과립이 관찰되었다. 이상의 결과로 보면 사람 태생기 중 고환의 간질세포는 다른 동물에서와 같이 유사한 분화과정을 거친다고 할 수 있다. The Leydig cell found within the interstitium of the testis is important in the spermatogenesis. The differentiation of Leydig cell, even though relatively well known in animals, is not fully elucidated yet in human. In the present study, human fetal testes ($14\sim27$ weeks) obtained from artificially induced abortions legally without gross malformations were studied using light and transmission electron microscopy to make clear the differentiation process of Leydig cells in humans. Leydig cells could be classified as 4 types: fusiform, light, dark, and degenerating cells. The most immature cell was the fusiform cells found frequently at 14 weeks, which seemed to differentiate successively into light and dark cells. Light cells were most frequently found at 17 weeks and dark cells at 24 weeks. Light cells were found to have mitochondria and smooth endoplasmic reticuli (sER) most prominently than any other cell types. The lumen of sER became to be expanded with age. Some electron-dense inclusions were observed in the mitochondrial matrix of the dark cells. Lipid droplets found more in light cells than dark cells were most prominent at 16 weeks and gradually decreased after 20 weeks. Glycogen particles were rich in dark cells. Degenerating cells were most frequently found at 27 weeks. From the results, it is suggested that Leydig cells in human fetal testes undergo similar differentiation process af in animals.

태아 대장 , 대장선종 및 대장암 환자에서 점소 당항원 Tn , sTn의 발현

손승우(Seung U Son),박언섭(Eon Sub Park),장세경(Sae Kyung Chang) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.1

N/A Background/Aims: Tn, Sialosyl-Tn(sTn) mucin core carbohydrate antigens are specific for mucin-type protein-linked N-acetylgalactosamine, which are involved in early steps of 0-linked glycosylation of mucin synthesis. These antigens have been shown previously to be highly sensitive and specific markers of colorectal cancer. Methods: We have studied that the expression of both antigens in terms of polyp cancer sequence and oncodevelopmental nature in Korean fetal and normal adult colon, colorectal polyps, transitional mucosa and carcinoma using monoclonal antibody CU l (Tn) and TKH2 (sTn) by immunohistochemical method. Results: Tn antigen was expressed in supranuclear cytoplasm of surface epithelial cells, and sialosyl-Tn antigen was in that of crypt bases and mucin lakes. Tn, sTn were expressed in 30%, 80% of 10 fetal colon, 20%, 10% of l0 normal adult colon, 8S%, 23% of l3 colon polyps and 74%, 65% of 23 transitional mucosa, 40%, and 84% of 43 colorectal adenocarcinoma respectively. Conclusions: These results suggest sTn carbohydrate antigen is a more useful tumor marker of colon cancer at tissue level and shows more specific differentiation marker in terms of polyp-cancer sequence. (Korean J Gastroenterol 1995;27:46-54)

태아 고환에서 버팀세포의 미세형태학적 연구

이태진,윤삼현,김미경,박언섭,유재형,Lee, Tae-Jin,Yoon, Sam-Hyun,Kim, Mi-Kyung,Park, Eon-Sub,Yoo, Jae-Hyung 한국현미경학회 2001 Applied microscopy Vol.31 No.2

정상 성인 고환의 버팀세포(Sertoli cell)는 비분열세포이며, 정세관(seminiferous tubule)단면에서 비교적 불분명하게 관찰되고, 정세관 세포 성분의 $10\sim15%$를 차지하고 있다. 전자현미경적으로 버팅 세포는 특징적인 핵소체와 원형질막 및 세포질 소기관을 갖고 있다. 원형질막은 사춘기에 발달한 두 종류 즉 버팅세포와 버팀세포 및 버팅세포와 생식세포 사이의 세포연접을 가지고 있다. 그러나 태이에서 버팅세포의 정확한 미세구조에 대한 기술은 드물다. 이에 본 저자는 태아 고환의 발생 제 14주부터 제27주 사이의 17예를 수집하여 정상 미세구조를 확인하고, 태아기 버팀세포의 분화 양상을 알아보고자 하였다. 태아기에서 버팀세포와 생식세포 및 버팀세포와 버팀세포 사이의 세포연접은 부착반점과 비슷한 구조로 이루어져 있었고, 이들은 관찰 대상인 태령 제14주부터 관찰되었다. 태아기 버팅세포의 세포소기관의 발달은 전반적으로 미약하였다. 비교적 풍부하게 사립체가 태령 제14주부터 관찰되었고, 무과립세포질세망이 소수, 그리고 과립세포질세망이 비교적 풍부하게 관찰되었다. 지방소포의 수는 비교적 일정하게 관찰되었고, 포도당입자는 발생 단계에 따라 점차 증가하는 소견을 보였다. 미세섬유와 Charcot-Bottcher의 결정소체는 본 연구대상에서는 관찰되지 않았다. 결론적으로, 태아기의 버팀세포에서는 어른에서 관찰되는 특징적인 소견들이 관찰되지 않았으며, 어른과는 다소 다른 전자현미경 소견을 나타냈다. 하지만 버팅세포의 분화양상을 정확히 알기 위해서는 태령 제27주 이후부터 사춘기까지의 연구가 추가되어야 할 것으로 생각한다. Sertoli cells in the normal adult testis are nondividing cells, which are relatively inconspicuous on cross section of the seminiferous tubule and comprise about 10% to 15% of the tubular cellular elements. Ultrastructurally, Sertoli cells have characteristic nucleoli, plasma membrane, and cytoplasmic components. The plasma membrane has two types of intercellular junctions which are developed at puberty: junctions between adjacent Sertoli cells and Sertoli cell-germ ceil junction. However, the ultrastructural findings of Sertoli cells in human fetus is not fully elucidate yet. In the present study, human fetal testes ($14\sim27$ weeks) obtained from artificially induced abortions legally without gross malformation were studied using transmission electron microscopy to make clear the differentiation process of Sertoli cells in human. In human fetal testes from 14 weeks to 27 weeks, the cell junctions of Sertoli-germ cells and Sertoli-Sertoli cells are desmosome like structure and not tight junction or desmosome. The Overall intracytoplasmic organelles of Sertoli cells are relatively sparse. The mitochondrias are relatively abundant but no developed cristae. And the rough endoplasmic reticuli are abundant and smooth endoplasmic reticuli are sparse. The amount of lipid droplets are regularly observed in human fetal Sertoli cells. No microfilaments or Charcot-Bottcher's crystalloids are present. From the results, Sertoli cells in human fetal testes are somewhat different ultrastructural findings with puberty or adult. However, to make clear the differentiation process of Sertoli cells in human, further study for 28 weeks to puberty is required.

Diethylnitrosamine으로 유발된 흰쥐 간암 발생 및 진행과정에서 Factor Ⅷ R-Ag, Interleukin-1α, Interleukin-1β, Interleukin-6의 발현

박용검(Yong Keum Park),이혜선(Hye Sun Lee),박언섭(Eon Sub Park) 대한외과학회 2004 Annals of Surgical Treatment and Research(ASRT) Vol.66 No.2

극 저주파 전자기장이 N-bis(2-hydroxypropyl)nitrosamine과 Sulfadimethoxine에 의해 유발된 갑상샘 암에 미치는 영향

이수환(Soo Hwan Lee),박용진(Young Jin Park),박언섭(Eon Sub Park),김용석(Young Seok Kim),최유신(Yoo Shin Choi),김범규(Beom Gyu Kim),박성준(Sung Jun Park),정세민(Se Min Chong) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.3

Purpose: Long-term exposure to extremely low-frequency (60 ㎐) electromagnetic fields (ELF-EMF) raises the questions of the induction of biological effects including tumorigenesis. One mechanism through which ELF-MFS could influence neoplastic development is the imbalance of cellular proliferation and cell apoptosis. The present study investigated the effect of ELF-EMF on chemically-induced thyroid carcinogenesis in a rat. Methods: We examined cellular proliferation index measured by anti-Ki-67 antigen, apoptosis, apoptosis related proteins such as caspase 3 and p53, and cell cycle-related proteins (cyclin D1 and p21<SUP>WAF1</SUP>/Cip1). Forty Male F344 rats received a subcutaneous N-bis(2-hydroxypropyl)nitrosamine (DHPN, 2,800 ㎎/㎏) injection, and 1 week later were allowed free access to drinking water containing sulfadimethoxine (0.1%) for 12 weeks. Twenty rats were exposed by ELF-EMF. During the carcinogenesis, sequential histological changes from hyperplasia, adenoma, and ultimately to overt carcinomas were noted. Results: The exposure group of ELF-EMF, significantly increases the number size of carcinomas. Also, the proliferative and apoptotic indices were significantly increased in the ELF-EMF exposure group than in the control group. The caspase 3 protein expression did not show any significant changes between ELF-EMF group and control group. The p53 protein was not detected in both ELF-EMF exposure and control group. Among the cell cycle related proteins, cyclin D1, not p21<SUP>WAF1</SUP>/Cip1, was significantly increased in adenomas and carcinomas in ELF-EMF exposure group compared with the control group. Conclusion: Exposure of ELF-EMF effects on chemically-induced rat thyroid carcinogenesis as results of altered increase of cellular proliferation, apoptosis, and cyclin D1 expression.

N,N-Diethylnitrosamine으로 유도된 흰쥐 간암 발생 및 진행 과정에서 혈관내피세포 성장인자, 염기성 섬유아세포 성장인자와 Endostatin의 혈장과 간 조직에서의 변화

김범규(Beom Gyu Kim),차성재(Seong Jae Cha),박성준(Sung Jun Park),임현묵(Hyun Muk Lim),박언섭(Eon Sub Park) 대한외과학회 2005 Annals of Surgical Treatment and Research(ASRT) Vol.68 No.5

복벽 손상 백서에서 세가지 유형의 유착방지제의 효과

송형준(Hyung Jun Song),김종원(Jong Won Kim),박준석(Jun Seok Park),김용석(Yong Seok Kim),최유신(Yoo Shin Choi),김범규(Beom Gyu Kim),차성재(Sung Jae Cha),박성준(Sung Jun Park),장인택(In Taik Chang),박성일(Sung Il Park),박언섭(Eon Sub P 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.1

Purpose: Postsurgical adhesion formation is a significant clinical problem within every surgical specialty. Several adhesion barriers have been developed in the form of solution, membrane or film in an attempt to solve these problems. The purpose of the present study is to compare the efficacy of antiadhesive agents in the prevention of postsurgical adhesion formation in a standardized rat adhesion model. Methods: We examined forty Sprague-Dawley rats, which is a cecal abrasion with partial peritonectomy model. Three treatment groups (Group Ⅰ: Film-type Surgiwrap<SUP>ⓡ</SUP>, Group Ⅱ: Solution-type Guardix-sol<SUP>ⓡ</SUP>, Group Ⅲ: Membrane-type Interceed<SUP>ⓡ</SUP>), each consists of 10 rats, and a control group of 10 rats were used by saline. Ten days after surgery, the rats were killed, and the levels of adhesion were graded. Immunohistochemical staining for microvessel density (CD34, MVD) and macrophage (ED1) were performed in adhesion tissue. Results: The peritoneum adhesion mean scores are as follows: control group: 2.2±0.78, Group Ⅰ: 1.0±1.06, Group Ⅱ: 0.9±0.99, Group Ⅲ: 0.6±0.84. All treatment groups showed significantly less peritoneum adhesion (P=0.006), while there was no significant difference in each group. The intraperitoneal organs adhesion mean scores are as follows: control group: 2.8±0.91, Group I: 2.6±1.06, Group Ⅱ: 1.4±0.84, Group Ⅲ: 1.0±0.81. Group Ⅰ had no significant difference about intraperitoneal organs adhesion with control group, but Group Ⅱ and Group Ⅲ showed less intraperitoneal organs adhesion. The mean numbers of microvessel density are as follows: control group: 42.5±4.83, Group Ⅰ: 40.8±6.53, Group Ⅱ: 30.9±6.15, Group Ⅲ: 15.60±4.37, from which there was a significant difference between Group Ⅱ and Group Ⅲ with control group (P<0.001). The mean numbers of macrophage are as follows: control group: 223.3±33.12, Group I: 211.25±10.96, Group Ⅱ: 171.60±23.96, Group Ⅲ: 147.0±12.22, from which there was a significant difference between Group Ⅱ and Group Ⅲ with control group (P<0.001). Conclusion: In our animal model, three different types of antiadhesive agents (Surgiwrap<SUP>ⓡ</SUP>, Guardix-sol<SUP>ⓡ</SUP>, Interceed<SUP>ⓡ</SUP>) were effective in adhesion prevention, but Surgiwrap<SUP>ⓡ</SUP> had less antiadhesive effect for intraperitoneal organs adhesion. Membrane-type Interceed<SUP>ⓡ</SUP> had a better effect for microvessel density (MVD) and macrophage than solution-type Guardix-sol<SUP>ⓡ</SUP>.