영랑호의 (永郞湖) 육수학적 (陸水學的) 연구

조규송,박양생 ( Kyu Song Cho,Yang Saeng Park ) 한국하천호수학회 1969 생태와 환경 Vol.2 No.1·2

The study of typical brackish water, Yong-nang lake, which is situated at the east seaside of Korea had been done from March, 1964 to August, 1968. The results obtained are as follows. 1. The difference of water temperature between epilimnion and hypolimnion in the lake is deeply appeared in Aug., Sept., Oct., Dec., and Jan. The possible range of water temperature is average 14℃, and katotherm and thermocline is revealed in 2 -4m during the period toward autumn from summer, respectively. 2. Being accumulated the gyttia which is the source of non-organic salt at the bottom, anaerobic layer increasing take place in summer. The fact that the salinity of the lake in summer attained higher content at 2 -5m layers than annual average values is caused by the inflow of seawater. It is generally considered that a lots of residual salt at the bottom is connected with the decomposition of the bottom. The concentration of salt is unstable and is seasonally variable. So the lake belongs to mixo-mesobaline of the mixoeuhaline. 3. Because the range of pH value (6.6-7.2) is less than average pH value (7.0-8.0) that fishes are suitable to live in, it must be considered that the selection of fitter fish for raising is important. 4. The content of dissolved oxygen in the lake is 6.8cc/l in mid-summer and the condition is suitable to aquatic organisms or plankton for raising. 5. The content of N.P. and K salts is 0.2-0.35ppm, 0.02-0.05 ppm and 1.1-1.4 meq/l, respectively and the lake belongs to mesotrophic. 6. As the lake is brackish water, plankton flora of fresh water and sea water are mixed up each other. Chlorophyceae of phytoplankton is abundant in the lake because the species is often inflowed into the lake from around stream and paddy field. Bacillariophyceae is the abundant species of the Chlorophyceae and the species of fresh water and sea water is distributed in the peat. The fact that Rotifera, Clodocela and Copepoda of zooplankton is simmiliar with the content of fresh water ones but Notholca and Ceratium fusus is generally abundant is a characteristic of microfauna in brackish water.

Effect of Cadmium on Renal Organic Anion Transport In vitro

박용덕,최장규,박양생,Park, Yong-Duck,Choi, Jang-Kyu,Park, Yang-Saeng The Korean Physiological Society 1988 대한생리학회지 Vol.22 No.1

가토 신피질 절편에서 p-aminohippurate (PAH) 이동에 대한 카드뮴 이온(Cd)의 영향을 조사하여 다음과 같은 결과를 얻었다. 조직절편과 용액내의 PAH 농도비(S/M PAH)는 용액내에 Cd이 0.1 mM이상 존재할 때에 현저히 감소되었다. PAH influx에 대한 동력학적 분석결과 Cd에 의하여 influx의 최대이동율(즉 $V_{max}$)은 심하게 감소되지만 운반체와 기질간의 친화력(즉 $K_{m}$)에는 변화가 없었으며, 수동적 influx 역시 변화되지 않았다. 신피질조직의 산소 소모율은 1 mM Cd에 의하여 35% 가량 억제되었으며, 신피질 microsome 분획의 Na-K-ATPase 활성도는 Cd 농도가 $10^{-7}M$ 이상일 때 의의있게 억제되었다. 이상의 결과로 미루어 볼 때 신장조직이 카드뮴이온에 직접 노출될 경우 유기음이온의 능동적 이동능이 심하게 저해된다고 사료된다.

Actomyosin $Ca^{++}$ Activated Adenosinetriphosphatase 활성도에 대한 pH 및 온도의 영향

김희중,황애란,박양생,강두희,Kim, Hee-Joong,Hwang, Ae-Ran,Park, Yang-Saeng,Kang, Doo-Hee 대한생리학회 1977 대한생리학회지 Vol.11 No.2

The activity of the $Ca^{++}$ activated adenosinetriphosphatase (Ca-ATPase) of actomyosin systeme of rabbit and frog skeletal muscle has been studied at varying pH and temperature. The PH optima of the Ca-ATPase activity of the rabbit actomyosin was rather broad. Over the temperature range of $16-36^{\circ}C$ activity of the enzyme was not appreciably changed between pH 6.4-8.5; below and above which it rapidly reduced. The pH at the inflection point of the enzyme activity increased as temperature decreased, showing the ${\bigtriangleup}pH\;inflection/{\bigtriangleup}T$ of approximately $-0.018\;unit/^{\circ}C$. Consequently, $(OH^-)/(H^+)$ ratio at the inflection point was constant regardless of assay temperature. In the frog actomyosin systems the Ca-ATPase activity was not apparently altered between PH 6.4-7.0 when the incubation temperature was $15{\sim}30^{\circ}C$. Outside of this range of pH, however, the enzyme activity was dramatically decreased. The pH of the inflection point changed inversely with temperature. ${\bigtriangleup}pH\;inflection/{\bigtriangleup}T$ at the acidic side was approximately $-0.018\;unit/^{\circ}C$, whereas that at the alkaline side it was about $-0.037\;unit/^{\circ}C$. The Arrhenius Plot on the Ca-ATPase activity at constant $(OH^-)/(H^+)$ ratio of 1.0 was not linear, but showed break at arround $20^{\circ}C$ for both rabbit and frog actomyosin Preparations. From these results it was speculated that pH dependence of Ca-ATPase activity of rabbit actomyosin systems might reflect titrations of histidine-imidazole and -SH groups, and that of the frog actomyosin represents titrations of histidine-imidazole and lysyllysine ${\alpha}-NH_2$ groups.

Gentamicin 과 Tobramycin 의 Nephrotoxicity 에 관한 비교 연구

이호영 ( Ho Yung Lee ),박양생 ( Yang Saeng Park ) 대한내과학회 1985 대한내과학회지 Vol.29 No.2

Effects of SITS on Sodium Transport, Oxygen Consumption and Na-K-ATPase of the Frog Skin

이승묵,안미라,이승일,박양생,Lee, Seung-Mook,An, Mi-Ra,Lee, Syng-Ill,Park, Yang-Saeng The Korean Physiological Society 1983 대한생리학회지 Vol.17 No.1

적출된 개구리 피부에서 $Na^+$이동, 산소소모량 및 Na-K-ATPase활성도에 대한 SITS(4-acetamido-4'-isothiocyano-2, 2'-disulfonic stilbene)의 영향을 연구하였다. 피부를 통한 능동적$Na^+$이동을 추정하기 위하여 short-circuit current(SCC)를 측정하였으며, 산소소모량은 피부조직 및 분리된 표피조직에서 측정하였으며, Na-K-ATPase활성도는 표피조직의 $24,000{\times}g$분획에서 측정하였다. 피부를 통한 SCC는 10 mM SITS가 피부외측용액에 첨가될 때 급격히 하강하였으며, 내측용액에 첨가될 때는 20분정도 지난후 하강하기 시작하였으나 그 하강정도는 전자에 비해 약했다. SITS에 의한 SCC억제현상은 용액내에 $Cl^-$이 없을때도 나타났다. SITS에 의하여 피부 및 표피조직의 산소소모량은 억제되지 않았으나 표피조직분획내 Na-K-ATPase활성도는 심하게 억제되었다. 이상과 같은 성적은 SITS가 개구리 피부에서 능동적 $Na^+$이동을 강력히 억제함을 나타내는데, 이러한 억제작용은 이 약물이 주로 상피세포의 외측막에 작용하여 나타나는 것으로 사료되지만 $Na^+$펌프를 억제할 가능성을 전연 배제할 수는 없다. Effects of SITS (4-acetamido-4'-isothiocyano-2, 2'-disulfonic stilbene) on a $Na^+$ transport, tissue oxygen consumption and Na-K-ATPase activity were studied in isolated frog skin preparations. $Na^+$ transport was estimated by measuring the short-circuit current(SC) across the skin; oxygen consumption was measured in separated epidermis as well as in intact skin; and Na-K-ATPase was assayed in $24,000{\times}g$ fraction of epidermal homogenates. The SCC across the skin Was rapidly and substantially reduced in the presence of 10 mM SITS in the medium bathing the outside(mucosal) surface of the skin. When the drug was added to the inside(serosal) bathing medium, there was about 20 min delay for inhibition of SCC and the effect was less pronounced. The above effect of SITS was independent of the presence of $Cl^-$ in the bathing medium. The oxygen consumption of the skin tissue was not affected by SITS, but the Na-K-ATPase activity of a subcellular fraction of the skin was significantly inhibited. These results suggest that SITS retards $Na^+$ transport across the frog skin primarily by interfering $Na^+$ entry across the mucosal membrance of the epithelial cell, although an effect on $Na^+$ pump can not be ruled out completely.

Aminoglycoside 항생제에 의하여 유발되는 신장장애 기전에 관한 연구

이호영 ( Ho Yung Lee ),박양생 ( Yang Saeng Park ) 대한내과학회 1985 대한내과학회지 Vol.29 No.4

Actomyosin Ca⁺⁺ Activated Adenosinetriphosphatase 활성도에 대한 pH 및 온도의 영향

김희중(Kim, Hee-Joong),황애란(Hwang, Ae-Ran),박양생(Park, Yang-Saeng),강두희(Kang, Doo-Hee) 대한생리학회 1977 대한생리학회지 Vol.11 No.2

The activity of the Ca⁺⁺ activated adenosinetriphosphatase (Ca-ATPase) of actomyosin systeme of rabbit and frog skeletal muscle has been studied at varying pH and temperature. The PH optima of the Ca-ATPase activity of the rabbit actomyosin was rather broad. Over the temperature range of 16-36℃ activity of the enzyme was not appreciably changed between pH 6.4-8.5; below and above which it rapidly reduced. The pH at the inflection point of the enzyme activity increased as temperature decreased, showing the ΔpH inflection/ΔT of approximately -0.018 unit/℃. Consequently, (OH^-)/(H⁺) ratio at the inflection point was constant regardless of assay temperature. In the frog actomyosin systems the Ca-ATPase activity was not apparently altered between PH 6.4-7.0 when the incubation temperature was 15~30℃ . Outside of this range of pH, however, the enzyme activity was dramatically decreased. The pH of the inflection point changed inversely with temperature. ΔpH inflection/ΔT at the acidic side was approximately -0.018 unit/℃, whereas that at the alkaline side it was about -0.037 unit/℃. The Arrhenius Plot on the Ca-ATPase activity at constant (OH^-)/(H⁺) ratio of 1.0 was not linear, but showed break at arround 20℃ for both rabbit and frog actomyosin Preparations. From these results it was speculated that pH dependence of Ca-ATPase activity of rabbit actomyosin systems might reflect titrations of histidine-imidazole and -SH groups, and that of the frog actomyosin represents titrations of histidine-imidazole and lysyllysine α-NH₂ groups.

개구리 피부의 Sodium 이동, 산소 소모량 및 Na-K-ATPase에 대한 SITS의 영향

이승묵(Lee, Seung-Mook),안미라(An, Mi-Ra),이승일(Lee, Syng-Ill),박양생(Park, Yang-Saeng) 대한생리학회 1983 대한생리학회지 Vol.17 No.1

적출된 개구리 피부에서 Na⁺이동, 산소소모량 및 Na-K-ATPase활성도에 대한 SITS(4-acetamido-4 -isothiocyano-2, 2 -disulfonic stilbene)의 영향을 연구하였다. 피부를 통한 능동적Na⁺이동을 추정하기 위하여 short-circuit current(SCC)를 측정하였으며, 산소소모량은 피부조직 및 분리된 표피조직에서 측정하였으며, Na-K-ATPase활성도는 표피조직의 24,000 X g분획에서 측정하였다. 피부를 통한 SCC는 10 mM SITS가 피부외측용액에 첨가될 때 급격히 하강하였으며, 내측용액에 첨가될 때는 20분정도 지난후 하강하기 시작하였으나 그 하강정도는 전자에 비해 약했다. SITS에 의한 SCC억제현상은 용액내에 Cl^-이 없을때도 나타났다. SITS에 의하여 피부 및 표피조직의 산소소모량은 억제되지 않았으나 표피조직분획내 Na-K-ATPase활성도는 심하게 억제되었다. 이상과 같은 성적은 SITS가 개구리 피부에서 능동적 Na⁺이동을 강력히 억제함을 나타내는데, 이러한 억제작용은 이 약물이 주로 상피세포의 외측막에 작용하여 나타나는 것으로 사료되지만 Na⁺펌프를 억제할 가능성을 전연 배제할 수는 없다. Effects of SITS (4-acetamido-4 -isothiocyano-2, 2 -disulfonic stilbene) on a Na⁺ transport, tissue oxygen consumption and Na-K-ATPase activity were studied in isolated frog skin preparations. Na⁺ transport was estimated by measuring the short-circuit current(SC) across the skin; oxygen consumption was measured in separated epidermis as well as in intact skin; and Na-K-ATPase was assayed in 24,000 X g fraction of epidermal homogenates. The SCC across the skin Was rapidly and substantially reduced in the presence of 10 mM SITS in the medium bathing the outside(mucosal) surface of the skin. When the drug was added to the inside(serosal) bathing medium, there was about 20 min delay for inhibition of SCC and the effect was less pronounced. The above effect of SITS was independent of the presence of Cl^- in the bathing medium. The oxygen consumption of the skin tissue was not affected by SITS, but the Na-K-ATPase activity of a subcellular fraction of the skin was significantly inhibited. These results suggest that SITS retards Na⁺ transport across the frog skin primarily by interfering Na⁺ entry across the mucosal membrance of the epithelial cell, although an effect on Na⁺ pump can not be ruled out completely.

N-ethylmaleimide(NEM)가 개구리 피부의 Na⁺ 이동에 미치는 영향

송선옥(Song, Sun-Ok),정노팔(Jung, Noh-Pal),박양생(Park, Yang-Saeng) 대한생리학회 1979 대한생리학회지 Vol.13 No.1

Studies have been conducted using isolated surviving skin of Rana temporalia in an attempt to evaluate the effect of N-ethylmaleimide (NEM) on the epithelial Na⁺ transport. Active transport of Na⁺ across the skin was estimated by measuring short circuit current (SCC). NEM administered to the outside surface of the skin in concentration of 0.5 X 10^-4-2.5 X 10^-4M induced 20~40% increase during the first 30 mintues, followed by a gradual reduction in SCC. With NEM above 4 X 10^-4M, SCC was inhibited from the beginning. Qualitatively similar results were obtained when NEM was added to the inside bathing medium. However, the concentration of NEM for a similar effect was much higher with the drug in the inside bathing medium than in the outside bathing medium. The oxygen consumption of the skin was inhibited by NEM of above 10^-4M, the effect being of approximately the same magnitude as that on SCC. The activity of Na⁺-K⁺ ATPase of the skin was not inhibited by NEM below 10^-3M, but it was dramatically reduced with 1.2 X M NEM. The effects of NEM (10^-4M) on the SCC and oxygen consumption could be eliminated by adding cysteine (10^-4-10^-3M) in the medium, indicating that the SH group is involved in the action of NEM in the frog skin. On the basis of these results, the mode of action of NEM on the Na⁺ transport across the frog skin was discussed.

한국 해녀의 영양섭취 상태 및 에너지 균형에 관한 연구

주순재(Soon Jae Joo),이기열(Ki Yull Lee),이양자(Yang Cha Lee),박양생(Yang Saeng Park) 韓國營養學會 1983 Journal of Nutrition and Health Vol.16 No.4