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수입산 관상어로부터 분리된 tetracycline 내성 Aeromonas spp에 tet (M) 및 tet (G) 유전자의 특성 분석
박신후 ( Shin Hoo Park ),전려진 ( Lyu Jin Jun ),조기택 ( Ki Taek Cho ),진지웅 ( Ji Woong Jin ),정현도 ( Hyun Do Jeong ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.3
In this study, the molecular structures of tet(M) and tet(G) carried by tetracycline (Tc) resistant bacteria in intestinal microflora from the imported ornamental fish were characterized and compared with each other depend on the imported countries. Of the total isolates, approximately 8.9% of the Ent-lac+ (lactose fermentative bacteria on coliform media) Tc resistant isolates in fish from three different countries, Singapore, Taiwan and Brazil, were appeared to contain tet (M). Three representative isolates of different countries, Aeromonas spp. JSM-1 (Singapore), JTM-1 (Taiwan) and JBM-1 (Brazil), were isolated and analyzed the molecular structures of tet (M) gene. Interestingly, partial sequence tet (M) genes (1099 bp) in JBM-1 (Brazil) showed 99.5% homology with the tet (M) found in the Vibrio spp. RV16 isolate, obtained from marine fish in Korea and known to carry Tn1545 parent type of tet (M). In contrast, tet (M) gene JSM-1 and JTM-1 showed mosaic structure of Tn1545 and Tn916, and 100% homology with each other. It may suggest the presence of various characteristics in terms of tet (M) gene structure. The determined sequence of the tet(G) from Aeromonas spp. JSG-1 and JBG-1 isolated from Singapore and Indonesia ornamental fish respectively showed similar nucleotide sequence homology but revealed a few nucleotide changes in comparison with the sequence of the prototype tet (G) gene (S52437 in GenBank).
박신후 ( Shin Hoo Park ),김호종 ( Ho Jong Kim ),정재연 ( Jae Yeon Jeong ),홍민정 ( Minjung Hong ),김나영 ( Nayoung Kim ),박종연 ( Chong Yon Park ),신의철 ( Euichul Shin ) 대한보건협회 2021 대한보건연구 Vol.47 No.1
연구목적 : 본 연구는 장애인과 비장애인의 치과의료 이용 현황을 파악 및 비교분석하고, 치과의료 이용과 관련된 특성을 보정 후 장애가 치과의료 이용 접근성에 미치는 영향을 파악하고자 하였다. 연구방법 : 2016년도 국민건강보험공단의 건강정보자료를 이용해서 장애인 251,497명, 비장애인 4,888,249명을 선별하고, 장애가 치과의료 이용 접근성에 미치는 영향을 분석하기 위해 통제변수인 성별, 연령, 의료보장유형, 소득수준, 지역, 흡연, 고위험 음주, 만성질환 여부를 통제한 상태에서 다중 로지스틱 회귀분석(multiple logistic regression)을 실시하였다. 연구결과 : 치과의료 이용률은 장애인 42.0%, 비장애인 43.2%로 장애인이 비장애인 보다 0.97배 낮게 나타났다. 그리고 통제변수를 통제한 상태에서 로지스틱 회귀분석 결과, 치과의료 이용 접근성과 관련된 특성을 통제하였을 때 장애인이 비장애인보다 치과의료 이용을 경험할 확률이 0.97배 낮은 것으로 나타났다. 결론 : 이상의 결과를 종합하여 살펴보면, 장애인은 보정 후에도 비장애인보다 치과의료 이용을 경험할 확률이 낮아 장애인의 치과의료 접근성이 낮은 것을 확인 할 수 있었다. 따라서 장애인의 치과의료 이용에 대한 접근성을 높이기 위한 정책적 방안이 필요하다. Background : The purpose of this study was to compare the current status of dental service utilization between People with Disabilities and People without Disabilities, and to investigate the effects of disability on access to dental service after adjusting the characteristics related to dental service utilization. Methods : Data used in this study from National Health Insurance Service 2016 health information data. Sample size were 251,497 People with Disabilities and 4,888,249 People without Disabilities. Analysis method in this study was multiple logistic regression analysis in which control variables were gender, age, health insurance type, income level, region, smoking, high-risk drinking and chronic disease. Results : Dental service utilization rate was 42.0% for the People with Disabilities and 43.2% for the People without Disabilities, which was 0.97 times lower than for the People without Disabilities. The result of logistic regression analysis with controlled variables indicated that the People with Disabilities was 0.97 times less likely to experience dental care than the People without Disabilities when the characteristics related to access to dental service utilization were controlled. Conclusion : In summary, People with Disabilities are less likely to experience dental service utilization than People without Disabilities, even after adjusting. Therefore, policy measures are needed to increase access to dental service utilization for the People with Disabilities.
2015년 제주도 양식 넙치(Paralichthys olivaceus)를 대상으로 한 VHS 및 RSIVD 모니터링
박현경 ( Hyun Kyung Park ),전려진 ( Lyu Jin Jun ),김승민 ( Seung Min Kim ),박명애 ( Myoung Ae Park ),조미영 ( Mi Young Cho ),황성돈 ( Seong Don Hwang ),박신후 ( Shin Hoo Park ),정현도 ( Hyun Do Jeong ),정준범 ( Joon Bum Jeong ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.2
In this study, disease surveillance was performed to monitor the prevalence of viral haemorrhagic septicaemia virus (VHSV) and red seabream iridovirus (RSIV) in olive flounder, Paralichthys olivaceus in 2015. The fish samples were collected in March (60 farms), May (55 farms) and July (52 farms) from different farms in Jeju. Reverse transcription polymerase chain reaction (RT-PCR) (VHSV) or PCR (RSIV) results showed that VHSV detected in 2 farms, but RSIV has not been detected in any farms. The sequences of the nucleocapsid protein (N) and gly-coprotein (G) gene of the 2 VHSV isolates were successfully sequenced. Phylogenetic analysis was included VHSV isolates reported here together with a representative VHSV isolates available in GenBank. Phylogenetic analysis in-dicated that most of Korea VHSV isolates were closely related to the Japan and China genotype Ⅳa which is clearly distinct from the North American genotype Ⅳb.
저온 대기압 플라즈마를 이용한 해수 및 어류 병원성 세균 3종에 대한 살균소독효과
김수진 ( Soo-jin Kim ),박신후 ( Shin-hoo Park ),지보영 ( Bo-young Jee ),김영재 ( Yong-jae Kim ),권문경 ( Mun-gyoeng Gwon ) 한국어병학회 2020 한국어병학회지 Vol.33 No.1
Fish bacterial diseases have spread and caused serious problem for cultured marine fish in Korea. The important bacterial disease affecting mariculture such as olive flounder (Paralichthys olivaceus) are caused by Edwardsiella tarda, Vibrio scophthalmi and Streptococcus parauberis. For the bacterial disease protection in aquaculture industry, the water treatment is needed in aquaculture system. During the last decades atmospheric pressure non-thermal plasma in contact with liquids have received a lot of attention of environmental and medical application. In this study, we determined the disinfectant effect in seawater and three major fish bacterial disease pathogens by using low frequency plasma treatment. Three fish bacteria (E. tarda, V. schophthalmi, S. parauberis) were not detected within 16 min, 150 min and 270 min of 20 L, 500 L and 1 ton seawater post low frequency plasma treatment, respectively. Three major fish bacterial disease pathogens were not detected within 2 min after the low frequency plasma treatment, suggesting that the low frequency plasma possess disinfectant effectiveness.