Hematin : An Inhibitor of Erythrocyte NAD Nucleosidase

박시원,조용호,안태수,이민화 ( Sie Won Park,Yong Ho Cho,Tae Soo Ahn,Min Wha Lee ) 한국생화학분자생물학회 (구 한국생화학회) 1969 BMB Reports Vol.2 No.2

조릿대 추출물의 HL60 세포와 L1210 세포에 대한 세포독성 및 활성산소 소거효소

박시원 ( Sie Won Park ),( Jee Hee Kim ) 상명대학교 자연과학연구소 2003 自然科學硏究 Vol.11 No.-

Farnesol이 ICR 마우스의 대식세포의 증식, O2-, ROS 효소 (SOD, GPx), RNS (iNOS) 효소활성에 미치는 영향

박시원 ( Sie Won Park ) 상명대학교 자연과학연구소 2005 自然科學硏究 Vol.15 No.-

The current study was performed to find out the immunestimuating anticancer activity of farnesol, one of the monoterpenes. Farnesol showed generally the proliferating effect on the cell number of macrophages from ICR mouse. The most prominent proliferating effect was found to be 135.7% of control in 1.0 μg/ml for 48 hr. Such effect was accompanied by the coincident alterations of RNS (reactive nitrogen species) related enzymes activities such as inducible nitric oxide synthase (iNOS) and O2- ion together with and ROS (reactive oxygen species) related enzymes activities such as superoxide dismutase(SOD) and glutathione peroxidase (GPx). When L1210 cells was cultured in the presence of farnesol (1.0 μg/ml for 48 hr), O2- ion, SOD and GPx activities were increased considerably with values of 178.4 %, 275,4 %, 175.3% respectively. In addition, farnesol exhibited cytotoxic effect (76.4%) on L1210 cell in 1.0 μg/ml for 48 hr.

덱사메사돈이 흰쥐의 뇌의 푸로스타글란딘 D2 함량에 미치는 영향

박시원,이상섭 ( Sie Won Park,Sang Sup Lee ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.3

The present study was performed to investigate the effect of dexamethasone on the basal level of PGD₂ of rat brain. Primarily, several steroid hormones were administered orally for one week (10 ㎎/ℓ drinking water) and dexamethasone thereof was shown to have the most strongly decreasing effect (40%) on the level of PGD₂ of the whole brain. When dexamethasone was administered orally to find out time dependency, it was demonstrated that there was a steadily increasing abatement of PGD₂ in the whole brain from 1st day onward to 7th day of uptake. In a similar way, there was also a fast reduction of PGD₂ content with maximum value of 35% by route of intraperitoneal injection. Furthermore, this result of PGD₂ decrease by injection showed typical circadian rhythm with peak value (2.4 ng of PGDZ/whole brain) at 5 : 00 PM. In an attempt to observe the dose dependency, this steroid was administered intraperitoneally in concentrations of 10 ㎍ to 1,000 ㎍/100g body weight. According to the injected dose, there was a gradual increment of decreasing effect of dexamethasone rather dose dependently to the concentration of 500 ㎍/100g body weight. As the effect of dexamethasone on the level of PGD₂ was very evident, we dissected the brain into 13 parts in order to find out the responsible area for specially reduced content of PGD₂ in the brain. However, the obtained data showed that dexamethasone exerts the decreasing effect on the basal level of PGD₂ generally with similar ratio (18∼29%) in almost all areas. This result implied that dexamethasone may be involved in the physiological homeostasis by feed-back control acting on adrenal-hypothalamus axis as known already and additionally may take part in some other central nervous functions via PGD₂ derived from cell membrane by being distributed overally.

노화과정에 따른 뇌신경의 핵단백질의 변화

박시원 ( Sie Won Park ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1

Rat cerebellum nuclei showed some significant changes of non-histone chromosomal protein (NHCP), a kind of chromosomal protein, as a function of age by electrophoresis profile analysis. Non-histone chromosomal protein was found to be prominent in peak numbers 23, 24 and 37 during developmental stages generally. In adult cerebellum, peak numbers 1, 4, 22 and 26 were distinct as compared with those of neonatal or fetal rats. Further, electrophoresis profile of high mobility group (HMG) protein included in the nonhistone chromosomal protein category revealed the presence of HMG bands 1, 2 and 17 in both neonatal and adult stages but HMG 14 band was distinct in adult cerebellum exceptionally. The HMG protein pattern obtained from nerve cell types demonstrated no much difference between the neuron and glia cell rich found fractions of adult cerebellum.

기아를 일으킨 흰쥐의 생명연장에 미치는 천연약물의 효과

박시원(Sie Won Park),이현아(Hyun Ah Lee) 대한약학회 1995 약학회지 Vol.39 No.1

The current study was undertaken to select the crude drug showing life prolonging effect on the starved rats(Sprague-Dawley) and elucidate the reaction mechanism there of. As distilled water extracts prepared from twenty kinds of natural drugs were administered orally to female starved rats, Bupleuri Radix demonstrated the most prominent effect with 34.6% increment in the survival time. To investigate underlying mechanism of life prolonging effect of Bupleuri Radix, the concentrations of adrenocorticosteroids(corticosterone, testosterone and aldosterone) and enzyme activities of superoxide dismutase(SOD), glutamate oxaloacetate transaminase(GOT), glutamate pyrurate transaminase(GPT) were assayed in the serums of rats starved for 2, 4 and 6 days respectively. The results manifested much elevated values of corticosterone, aldosterone(2 days) and rather decreasing tendency afterwards, specially in the late periods of starvation(6 days). With respect to such a considerable changes according to starvation periods, Bupleur Radix restored these values much near to normal suggesting that Buplerum Radix may play a life prolonging action during starvation probably through reinforcing the homeostatic properties of corticosteroids and some enzyme activities.

토마토의 성장과정에 따른 카탈리아제 (Catalase) 활성에 관한 연구

박시원,김덕심,Sie Won Park,Duk Shim Kim 대한화학회 1993 대한화학회지 Vol.37 No.12

Catalase activities were assayed on the leaves and roots of Tomato (Lycopersicum esculentum) as a function of age for 1 year after germination. The enzyme activities of root tissue demonstrated to be insignificant through all stages of development. On the other hand, the catalase activities of leaf peroxisomal fractions showed remarkable changes with peak value of 76 ${\mu}mol$/ml/min at germination stage within 2 weeks growth, 7.2 ${\mu}mol$/ml/min at adult stage around 4∼5 months growth and very small activities at aged period around 11∼12 months growth. It is suggested that there may be two factors concerning such catalase activities in relation with age, firstly the glyoxisomal reactions including glyoxylate cycle and $\beta$-oxidation at germination stage and secondly photosynthesis hereafter seemed to affect age dependent changes of catalase activity by producing coincident amount of $H_2O_2$. In addition, NADPH coenzyme was found to have ability to restore catalytic acitivity of inactivated catalase (compound II) at all stages of development except old age, indicating NADPH would play a role as catalase protector against deleterious substrate,$H_2O_2$. 토마토(Lycopersicum esculentum)를 파종하여 1년간 성장시키면서 성장기간에 따른 잎과 뿌리의 catalase 활성 변화를 측정하였다. 뿌리에 있어서의 catalase 활성은 잎의 경우에 비해 전반적으로 매우 미약하였다. 반면에 잎의 catalase 활성은 매우 현저한 변화 양상을 나타냈는데 주로 peroxisomal 분획에 존재하였고 특히 발아 후 2주 이내의 발아초기에 76 ${\mu}mol$/ml/min의 최고 활성은 기점으로 하여 급격히 감소했다가 성장기 4 ∼ 5 개월에 즈음하여 상당히 증가된 활성을 보였으며 11 ∼ 12개월째 노화기에 가장 저조한 활성을 보였다. 이와 같이 성장시기에 따라서 변화하는 catalase 활성에 영향을 미치는 인자로서 첫째 발아기에는 glyoxylate cycle과 $\beta$-oxidation이 일어나는 glyoxisomal 반응 그리고 둘째 발아기 이후에는 광합성 반응이 각각 해당시기에 $H_2O_2$를 발생시키는 반응으로 작용할 것으로 간주된다. 이 외에도 NADPH는 불활성화된 catalase(compound II)를 다시 활성화시켜 해로운 기질인 $H_2O_2$로부터 catalase 보호제로서의 역할을 함으로써 토마토의 생장기간 전체에 걸쳐 작용하는 것으로 나타났다.

덱사메사돈이 흰쥐의 뇌의 푸로스타글란딘 $D_2$ 함량에 미치는 영향

박시원,이상섭,Park, Sie-Won,Lee, Sang-Sup 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.3

본 연구는 덱사메사돈이 흰쥐의 뇌의 $PGD_2$(prostaglandin $D_2$)의 기본 준위에 미치는 영향을 관찰하고자 행하였다. 우선 몇가지 스테로이드 호르몬을 식수에 분산 용해 (10 mg/l)시켜 일주일간 경구투여했을 때 전반적으로 전뇌의 $PGD_2$의 함량이 감소되었으며 그 억제 효과는 덱사메사돈의 경우 40%로 가장 현저하였다. 덱사메사돈을 경구투여하여 얻은 시간의존표에 의하면 투여 첫날부터 제 칠일까지 계속 $PGD_2$의 함량 감소 추세가 증가하였다. 덱사메사돈을 복강내 주사로 투여했을 때도 $PGD_2$의 함량 억제가 신속히 일어났으며 투여후 8시간째에 35%의 최고 억제율을 보였다. 아울러 $PGD_2$의 함량 자체가 오후 5시에 최고치(2.4 ng)를 나타내는 일일주기성을 보였다. 용량의존성을 관찰하기 위해서 체중 100 g당 $10\;{\mu}g$에서 $1,000\;{\mu}g$까지 복강내 주사를 행하였으며, $200\;{\mu}g$ 농도까지는 억제효과의 상관성이 나타났다. 이상과 같은 덱사메사돈의 $PGD_2$ 함량에 대한 억제효과가 뇌의 어느 부위에서 가장 현저한지 밝히기 위하여 뇌를 13개 부분으로 나누어 측청한 바 모든 부분에 있어서 비교적 균등하게(18~29%) $PGD_2$의 함량이 감소되었다. 이들 결과로부터 덱사메사돈은 부신-시상하부로 연결되는 부신피질호르몬 분비에 대한 피드백조절자로서의 작용 이외, 전뇌에 고르게 분포되어 그 부분의 세포막에서 유래되는 $PGD_2$의 함량을 감소시킴으로써 중추신경계의 기능에 영향을 미치리라 유추된다. The present study was performed to investigate the effect of dexamethasone on the basal level of $PGD_2$ of rat brain. Primarily, several steroid hormones were administered orally for one week (10 mg/l drinking water) and dexamethasone thereof was shown to have the most strongly decreasing effect (40%) on the level of $PGD_2$ of the whole brain. When dexamethasone was administered orally to find out time dependency, it was demonstrated that there was a steadily increasing abatement of $PGD_2$ in the whole brain from 1st day onward to 7th day of uptake. In a similar way, there was also a fast reduction of $PGD_2$ content with maximum value of 35% by route of intraperitoneal injection. Furthermore, this result of $PGD_2$ decrease by injection showed typical circadian rhythm with peak value (2.4 ng of $PGD_2$/whole brain) at 5:00 PM. In an attempt to observe the dose dependency, this steroid was administered intraperitoneally in concentrations of $10\;{\mu}g$ to $1,000\;{\mu}g$/100g body weight. According to the injected dose, there was a gradual increment of decreasing effect of dexamethasone rather dose dependently to the concentration of $500\;{\mu}g$/100g body weight. As the effect of dexamethasone on the level of $PGD_2$ was very evident, we dissected the brain into 13 parts in order to find out the responsible area for specially reduced content of $PGD_2$ in the brain. However, the obtained data showed that dexamethasone exerts the decreasing effect on the basal level of $PGD_2$ generally with similar ratio (18~29%) in almost all areas. This result implied that dexamethasone may be involved in the physiological homeostasis by feed-back control acting on adrenal-hypothalamus axis as known already and additionally may take part in some other central nervous functions via $PGD_2$ derived from cell membrane by being distributed overally.

감나무의 배 배양법에 의한 기내 증식

박시원(Sie Won Park) 한국생물공학회 1995 KSBB Journal Vol.10 No.1

Farnesol의 ICR 생쥐 Macrophages의 증식에 미치는 영향

박시원 ( Sie Won Park ) 상명대학교 자연과학연구소 2004 自然科學硏究 Vol.13 No.-