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쪽(Polygonum tinctortium) 세포의 형질전환 및 쪽 세포에서 Sodium Butyrate가 Green Fluorescent Protein 발현에 미치는 영향
박성길,정충식,이종진,이윤형,정인식,Park, Sung-Kil,Chung, Choong-Sik,Lee, Jong-Jin,Lee, Youn-Hyung,Chung, In-Sik 한국응용생명화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4
쪽(Polygonum tinctortium) 세포에서 외래 단백질 발현을 검토하기 위하여 green fluorescent protein(GFP)가 내재하는 pCAMBIA1302를 형질 전환시켰으며 Western blot 분석에 의해 GFP의 발현을 확인하였다. Sodium butyrate가 GFP생성에 미치는 효과를 검토한 결과, 10 mM에서 세포성장이 지연되었으며, 15 mM 이상에서는 정지되었다. 세포 내 GFP 생성량은 세포 접종 후 3일째 5 mM sodium butyrate를 첨가하였을 때가 0일째 처리에 비해 120% 증가하였다. 또한 접종후 3일 후 5 mM의 sodium butyrate를 처리한 경우가 10 mM의 경우보다 GFP의 수율이 50% 증가하였다. 본 실험을 통하여 세포 접종 후 3일째, 5 mM의 농도로 처리한 sodium butyrate가 외래 단백질의 발현을 효과적으로 증가시키는 결과를 확인하였다. To examine the expression of foreign protein in Polygonum tinctorium cells, plasmid pCAMBIA1302 encoding Green Fluorescent Protein(GFP) was used to transform the cells and the expression was confirmed using Western blot analysis. When the effect of sodium buryrate on the formation of GFP was examined, cell growth was retarded at the addition of 10 mM and was stalled at more than 15 mM. The amount of GFP production was increased by 15% when 5 mM of sodium butyrate was added at three-days after inoculation as compared to at 0-day. Moreover, when sodium butyrate was added at three-days after inoculation, the amount of GFP was increased by 50% at the addition of 5 mM of sodium butyrate as compared to 10 mM.
고립성폐결절의 경피적 바늘생검 이후 발생한 결핵성 흉수
구호석 ( Ho Seok Koo ),김태균 ( Tae Kyun Kim ),박성길 ( Sung Kil Park ),최상분 ( Sang Bun Choi ),김애란 ( Ae Ran Kim ),최상봉 ( Sang Bong Choi ),정훈 ( Hoon Jung ),박이내 ( I Nae Park ),허진원 ( Jin Won Hur ),이혁표 ( Hyuk Pyo Le 대한결핵 및 호흡기학회 2007 Tuberculosis and Respiratory Diseases Vol.63 No.3
A tuberculous pleural effusion may be a sequel to a primary infection or represent the reactivation of pulmonary tuberculosis. It is believed to result from a rupture of a subpleural caseous focus in the lung into the pleural space. It appears that delayed hypersensitivity plays a large role in the pathogenesis of a tuberculous pleural effusion. We encountered a 52 years old man with pleural effusion that developed several days after a CT guided percutaneous needle biopsy of a solitary pulmonary nodule. He was diagnosed with TB pleurisy. It is believed that his pleural effusion probably developed due to exposure of the parenchymal tuberculous focus into the pleural space during the percutaneous needle biopsy. This case might suggest one of the possible pathogeneses of tuberculous pleural effusion. (Tuberc Respir Dis 2007; 63: 268-272)
형질전환 담배 식물체에서 개똥쑥 Terpene Synthase의 발현
박성길,홍성현,최근원,이윤형,정인식 경희대학교 생명자원과학연구원 2002 硏究論文集 Vol.22 No.-
Expression of Artemisia annua terpene synthase in transgenic tobacco plants was examined. A full length cDNA encoding terpene synthase was cloned into pILTAB357 vector and transgenic tobacco plants were generated. The integration of this gene was analyzed by genomic DNA PCR and Southern blot analysis. The transcription of the terpene synthase gene in tobacco was confirmed by RT-PCR and northern blot analysis.
홍성현,박성길,이종민,한태룡,백영숙,정인식 경희대학교 생명자원과학연구원 1999 硏究論文集 Vol.20 No.-
홍화로부터 열에 의한 안정성을 검토하였다. 황색소의 분해반응은 산성, 중성, 염기성 조건에서 UV/Vis spectral measurement에 의해 조사되었다. 시간변화에 다른 흡광도를 측정해 본 결과 온도가 상승함에 따라 황색소의 분해되는 정도가 점차적으로 증가하는 것을 확인 할 수 있었다. 40℃에서 황색소의 분해 반감기는 pH3.0, pH7.0, 그리고 pH12.0에서 각각 112.1, 39.3 과 25.3 시간이었다. 이것은 황색소가 pH3.0에서 비교적 더 안정함을 말한다. pH3.0, 7.0 그리고 12.0에서 황색소의 activation energy는 각각 519, 15.0 그리고 12.3 kJ/mol이었다 We investigated thermal stability of yellow pigment from Carthamus tinctorious L. Decomposition reactions of yellow pigment were monitored at acidic, neutral and alkaline conditions by UV/Vis spectral measurement. Decomposition of yellow pigment increased as temperature increased Decomposition half lives of yellow pigment at 40℃ were 112.1 39.3, and 25.3 hrs at pH 3.0 , pH 7.0, and pH 12.0, respectively, indicating that yellow pigment is more stable at pH 3.0. The activation energies of yellow pigment at pH 3.0, pH 7.0, and pH 12.0 were 51.9, 15.0, and 12. 3 kJ/mol, respectively.
쪽(polygonum tinctorium) 세포의 형질전환 및 쪽 세포에서 Sodium Butyrate 가 Green Fluorescent Protein 발현에 미치는 영향
이종진,정인식,이윤형,정충식,박성길 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4
To examine the expression of foreign protein in Polygonum tinctorium cells, plasmid pCAMBIA1302 encoding Green Fluorescent Protein(GFP) was used to transform the cells and the expression was confirmed using Western blot analysis. When the effect of sodium butyrate on the formation of GFP was examined, cell growth was retarded at the addition of 10 mM and was stalled at more than 15 mM. The amount of GFP production was increased by 15% when 5 mM of sodium butyrate was added at three-days after inoculation as compared to at 0-day. Moreover, when sodium butyrate was added at three-days after inoculation, the amount of GFP was increased by 50% at the addition of 5 mM of sodium butyrate as compared to 10 mM.