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한국인 대장암 환자에서 serine/arginine-rich splicing factors 2 (SRSF2) and SRSF4 발현
김진휘(Jin-Whi Kim),박도심(Do-Sim Park),박원철(Won-Cheol Park),안소라(Sora Ahn),강동백(Dong-Back Kang),김근영(Keun-Young Kim),김용(Yong Kim) 대한종양외과학회 2016 Korean Journal of Clinical Oncology Vol.12 No.2
Purpose: Splicing factors play important roles in tumorigenesis. Serine/arginine-rich splicing factors 2 (SRSF2) and SRSF4 proteins, the members of SR family proteins, are dysregulated in various cancers. However, their protein expression levels and diagnostic values are unclear in colorectal cancer. Methods: We quantified the protein levels of SRSF2, SRSF4, and previously known colon cancer markers (heterogeneous nuclear ribonucleoprotein A1 [HNRNPA1] and carcinoembryonic antigen [CEA]) in tumor compared with adjacent normal-looking areas (non-tumor) of the colon in Korean patients with colon cancer using immunoblot analysis. Results: The protein levels of HNRNPA1 and CEA were remarkably increased in tumor compared to non-tumor tissue and up-regulated in all of the tumor samples. However, the protein levels of SRSF2 and SRSF4 in tumor tissue were reduced in contrast with those of non-tumor tissue. Conclusion: None of the SRSF proteins were significantly different between the low (≤II) and high (>II) stages.
만성 B형 간염 바이러스 감염자에서 Enhancer 1과 Pre-X Promotor 부위의 염기 다양성
송우건 ( Woo Kern Song ),김학철 ( Haak Cheoul Kim ),박도심 ( Do Sim Park ),조지현 ( Ji Hyun Cho ) 대한소화기학회 2003 대한소화기학회지 Vol.41 No.3
Background/Aims: Hepatitis B Virus (HBV) is a compact DNA virus with four genes which are controlled by 6 promotors and two enhancers during its replication. However, HBV has variability in its nucleotide sequence and thus, it can be classified into genotypes. Recently, it is suggested that nucleotide mutations in its sequence are related to chronic liver disease (CLD). Therefore, we analyzed relationships between mutations and disease severity in its Enh 1 and X-promoter regions (1021-1373). Methods: HBV DNA of the patients with chronic hepatitis (CH, n=30), liver cirrhosis (LC, n=25), and hepatocellular carcinoma (HCC, n=17) was amplified and sequenced. Then, we constructed a phylogenetic tree (PAUP4.0) and analyzed their mutation rates and specificity among the disease groups. Results: All analyzed sequences belonged to genotype C. The divergency rate was 1.71% and there was no difference in their mutation rates, but there were some hot-spots among the disease groups. The hot-spots were at nucleotides 1317, 1229, 1126, 1134, and 1041 in decreasing order. Between the groups of LC and HCC, there were higher mutation rates at nucleotides 1126 (A→C) and 1134 (T→C) in LC, and at nucleotides 1229 (G→A) and 1317 (A→G) in HCC. The mutations at nucleotides 1126 and 1134 (p< 0.001) were accompanied. Conclusions: The genotype of HBV in Korea is type C in all cases and there might exist the associated mutations and hot-spots related to CLD severity in Enh 1 and X-promoter regions. (Korean J Gastroenterol 2003;41:211-218)
혈액투석 환자에서 Advanced Oxidation Protein products(AOPP)의 농도에 영향을 주는 요인
차정민 ( Jeong Min Cha ),이강원 ( Kang Won Lee ),이유민 ( Yu Min Lee ),김현정 ( Hyun Jung Kim ),박도심 ( Do Sim Park ),안선호 ( Seon Ho Ahn ),송주흥 ( Ju Hung Song ) 대한신장학회 2009 Kidney Research and Clinical Practice Vol.28 No.5
Purpose: Advanced oxidation protein products (AOPP) has long been considered as a useful marker to estimate oxidative stress in the hemodialysis (HD) patients. However, it has not been clarified what clinical factors can affect the plasma level of AOPP in the HD patients. Based on these, We investigated the correlation between plasma AOPP level and clinical factor, known to be associated with oxidative stress, in the maintenance HD patients. Methods: Two groups (50 of normal healthy persons and 105 of stable HD patients) were independently subjected in this study, and statistical correlation between plasma AOPP level and several clinical factors were analyzed. Results: Plasma level of AOPP in the maintenance HD patients were higher than those in normal healthy group (52.11±16.08 μmol/L vs. 40.25±12.23 μmol/L, p<0.001). Plasma AOPP level of maintenance HD patients were significantly correlated with duration of hemodialysis, MDRD-GFR and daily urine volume. However, plasma level of AOPP in the maintenance HD patients were not affected by sex, diabetes, smoking, angiotensin-converting enzyme inhibitors or angiotensin II receptor blockers, and those were not correlated with age, CRP and serum ferritin. It was demonstrated by multiple regression analysis that daily urine volume was the most important clinical factor which could affect the plasma level of AOPP (beta=-0.255, p=0.017). Conclusion: These results suggest that maintenance of daily urine volume is likely to be critical to reduce oxidative stress in the maintenance HD patients.
만성 B형 간염의 젊은 남자에서 간조직내 HBcAg 발현과 HBV C-gene의 변이와의 연관성
김태현 ( Kim Tae Hyeon ),김학철 ( Kim Hag Cheol ),김용성 ( Kim Yong Seong ),염주진 ( Yeom Ju Jin ),손영우 ( Son Yeong U ),유교상 ( Yu Gyo Sang ),박도심 ( Park Do Sim ),조지현 ( Jo Ji Hyeon ),윤기중 ( Yun Gi Jung ),문형배 ( Mun Hye 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-
<배경 및 목적> HBV의 간세포의 손상은 직접적인 세포독성보다는 감염된 간세포에 숙주의 면역반응 결과로 알려져 있고 이러한 면역반응은 HBcAg이 표적항원으로 작용한다. 간조직내 HBcAg은 혈청 HBV DNA양과 밀접한 관련이 있는 것으로 알려져 있으나 혈청 HBV DNA가 높은 경우에도 발현되지 않는 경우를 조직의 절편의 크기로 설명하지만 충분한 절편에서도 발현되지 않는 경우도 있다. 또한 HBV의 염기서열은 사람마다 차이가 있어 발현되는 단백질에
신기능 저하 환자에서 위막성 대장염 발생의 임상적 특징
정성원 ( Sung Won Jung ),이유민 ( Yu Min Lee ),정덕은 ( Duk Eun Jung ),이재훈 ( Jae Hoon Lee ),김현정 ( Hyun Jung Kim ),이지은 ( Ji Eun Lee ),송주흥 ( Ju Hung Song ),박도심 ( Do Sim Park ),안선호 ( Seon Ho Ahn ) 대한신장학회 2009 Kidney Research and Clinical Practice Vol.28 No.2
목적: Clostridium difficile-associated diarrhea (CDAD)는 항생제 사용 후 장내세균 변화로 인해 C. difficile 독소 A에 의해 발생되는 위막성 대장염에 의한 것으로 알려져 있다. 본 연구는 대장내시경 후 조직검사를 통해 위막성 대장염으로 확진된 CDAD 환자에서 임상적 및 검사실적 소견의 분석을 통해 신기능의 저하가 CDAD 발생에 어떤 영향을 미치는지 알아보고자 하였다. 방법: 1998년 1월부터 2006년 12월까지 원광대학교 의과대학 병원에서 2일 이상 묽은 변과 고열이 발생한 환자에서 CDAD가 의심되어 C. difficile 독소 A를 시행하고 대장내시경을 통해 조직학적으로 CDCA로 확진된 환자 56명을 대상으로 신기능 저하, 당뇨 및 악성종양의 유무를 조사하여 항생제 투여 기간에 따른 CDAD 발생 빈도를 비교하였다. 결과: 연구 기간 동안 CDAD가 의심되어 C. difficile 독소 A를 검사한 환자는 452명이었으며, 이중 양성 환자 416명 (92.03%), 음성 환자 36명 (7.96%)이었다. 452명 중 대장내시경을 시행한 환자는 92명 (20.7%)이었고, 이중 조직검사에서 위막성 대장염으로 진단된 환자는 56명 (60.8%)이었다. 조직 검사로 진단된 위막성 대장염 환자 56명 (남자 26명; 여자 30명)이었고, 이중 신기능 저하 환자 8명 (14.2%), 당뇨환자 10명 (17.8%), 악성종양 환자 13명 (23.2%)이었다. 또한 독소 A가 음성인 환자는 36명 (64.3%)었다. 조직검사로 확진된 신기능 저하 환자 8명 중 5명 (62.5%)은 C. difficile 독소 A 검사에서 음성으로 신기능 저하 유무에 따른 독소 반응의 차이는 없었다 (62.5% vs. 64.6%). 신기능이 저하된 환자에서 당뇨 2명 (25%), 악성종양 환자 1명 (12.5%)이었다. CDAD 발생까지의 항생제 투여 기간은 신기능이 정상인 환자 보다 신기능이 저하된 환자에서 짧았고 (23.44±3.28일 vs. 11.13±2.386일, p=0.034), 당뇨 및 악성 종양 유무에 따른 비교에서 (20.85±2.221일 vs. 25.50±13.07일; 23.30±3.67일 vs. 16.31±2.31일) 차이가 없었다. 결론: 면역력이 저하된 당뇨 및 악성종양 환자에 비해 신기능이 저하된 환자에서 CDAD 발생까지의 항생제 투여 일수가 적어 신기능 저하 상태에서 특히 항생제 투여에 주의가 필요하다. 또한 항생제 투여 후 발생된 설사 환자에서 C. difficile 독소 A가 음성이라 할지라도 환자가 대장내시경을 시행할 수 있는 상태인 경우 반드시 대장내시경과 조직검사를 시행하여 CDAD를 감별해야 할 것으로 생각한다. Purpose: Clostridium difficile-associated diarrhea (CDAD) is a potentially life-threatening illness which has been shown to be more common and more severe in patient with chronic renal failure. The aim of this study was to investigate clinical characteristics of renal insufficiency patients with clostridium difficile-associated pseudomembranous colitis. Methods: We reviewed charts of fifty-six patients with clostridium difficile-associated pseudomembranous colitis, who have clostridial toxin A assay in stool and a diagnosis made on histology of colonic biopsies. Results: There was no difference in age, serum albumin, C-reactive protein (CRP) and negative incidence of clostridial toxin A between patients who had renal insufficiency with serum creatinine more than 1.5 mg/dL and those who did not. But duration of antibiotic use administered prior to development of the clostridium difficile infection was more shorter in patients with impaired renal function than in patients with normal renal function. Conclusion: These data suggest that it may take a short period to development of the clostridium difficile infection in patients with impaired renal function, and histologic evaluation by sigmoidoscopy should be performed to make a diagnosis in CDAD-suggested patients, who have impaired renal function and even negative clostridial toxin A.