고지방식이 마우스에서 3,5-dicaffeoylquinic acid의 항비만 효과

강진용(Jin Yong Kang),박선경(Seon Kyeong Park),김종민(Jong Min Kim),박수빈(Su Bin Park),유슬기(Seul Ki Yoo),한혜주(Hye Ju Han),김대옥(Dae Ok Kim),허호진(Ho Jin Heo) 한국식품과학회 2019 한국식품과학회지 Vol.51 No.1

이연구는 고지방식이로 인한 비만 및 대사장애에 대한 3,5-diCQA의 효과를 확인하는 동시에 클로로겐산과의 상대적인 생리 활성을 확인하였다. 클로로겐산은 이전에 보고된 연구결과에서처럼 고지방식이를 섭취하는 쥐의 체중 증가를 효과적으로 억제하였으며, 3,5-diCQA와 비교하였을 때에도 그 효과가 상대적으로 우수하였다. 내장지방의 무게를 측정한 결과 3,5-diCQA와 클로로겐산 모두 내장지방의 축적을 효과적으로 억제하였으며, 이러한 이유는 두 샘플 모두 지방조직에서의 AMPK의 활성화가 증가된 것과 관련이 있는 것으로 보인다. 또한 이 두 샘플은 비만으로 인해 발생하는 산화적 스트레스로부터 간조직을 보호하는 효과가 있었다. 그러나 간조직에서의 지방축적을 확인한 결과에서 클로로겐산은 여전히 간의 지방축적을 억제하는 것으로 확인되었지만 3,5-diCQA는 오히려 간의 지방축적을 증가시킨 것이 확인되었다. 이러한 결과는 3,5-diCQA가 간조직에서 Akt의 활성을 증가시킨 것과 관련이 있는 것으로 보이며 이 증가된 Akt로 인해 3,5-diCQA 그룹의 내당능 개선이 클로로겐산 그룹보다도 우수하였지만 간에서의 지방 축적을 증가시킨 것으로 판단된다. This study was performed to confirm the influence of chlorogenic acid (CGA) and 3,5-dicaffeyolquinic acid (3,5-diCQA) intake on problems caused by high-fat diet. CGA was more effective in suppressing weight gain than 3,5-diCQA. In contrast, 3,5-diCQA was more effective in improving glucose tolerance than CGA. In the biopsy, it was confirmed that CGA inhibited visceral fat and liver fat accumulation. 3,5-diCQA also inhibited visceral fat accumulation, but 3,5-diCQA increased liver fat accumulation. The liver fat accumulation induced oxidative stress, but 3,5-diCQA reduced oxidative damage through its antioxidant activity. The increased liver fat accumulation was because a 3,5-diCQA greatly increased Akt phosphorylation and decreased AMPK phosphorylation in the liver. Consequently, CGA was effective in alleviating the problems caused by high-fat diets, while maintaining normal balance. 3,5-diCQA also showed a positive effect on problems caused by high-fat diets, but it increased liver fat accumulation and thereby had negative consequences.

식도 (食道) 편평유두종 (扁平乳頭腫) 2예

강진경(Jin Kyung Kang),최흥재(Heung Jai Choi),박인서(In Suh Park),문영명(Young Myoung Moon),이상인(Sang In Lee),전재윤(Chae Yoon Chon),김원호(Won Ho Kim),박찬일(Chan Il Park),이강석(Kang Suk Lee),이광길(Kwang Kill Lee) 대한소화기학회 1984 대한소화기학회지 Vol.16 No.1

Benign tumors of esophagus arising either from mucosa or submucosa are rare pathological entities. Of the benign esophageal tumor, squamous papilloma is a distinct rare pathological peculiarity recently reported with increased frequency, giving no symptoms during life and discovered unusually and in cidentally during autopsy. Morphologically, squamous papilloma of the esophagus was benign sessile lesions of surface epithelium characteristically composed of finger-like projections of tissue lined by increased numbers of squamous cells and having cores of fibrovascular tissue. Although the etiology of squamous papilloma of esophagus remains uncertain, the most convincing etiology of humans remains chronic irritation from gastric acid reflux. Squamous papilloma of the esophagus have not been shown to be premalignant. They should be removed completely so that coexisting malignant conditions are not overlooked. Recently we had the opportunity to observe two cases of histologically demonstrated squamous papilloma of the esophagus. Our purpose here is to report these patients and to review the literatures.

간외담관 낭선종 ( Extrahepatic Biliary Cystadenoma )

강진경(Jin Kyung Kang),최흥재(Heung Jai Choi),박인서(In Suh Park),정재복(Jae Bock Chung),전재윤(Chae Yoon Chon),김원호(Won Ho Kim),박찬일(Chan Il Park),송시영(Si Young Song),박영년(Young Nyun Park),이경식(Kyoung Sik Lee) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.3

Cystic tumor of the intra- or extra- hepatic bile ducts is a rare but distinctive neoplasm, similar to that occurring in the pancreas or ovary. The cystadenomas of the extrahepatic biliary tree are extremely rare, and most of them are usually intrahepatic, and rarely diagnosed preoperatively. We report a 63-year-old woman admitted because of intermittent abdominal pain for 1 month. Abdominal ultrasonography revealed a 2.5 cm hypoechoic oval mass with hyperechoic rim in dilatecl common hepatic duct and common bile duct. Abdominal computerized tomography revealed a hypodense mass surrounded by a thick dense outer rim. A 2.5*1.5 cm filling defect hanging from the right hepatic duct with a stalk was noticed on endoscopic retrograde cholangiography (ERC). Under the diagnosis of benign tumor originating from extrahep:,stic bile duct, laparotomy was performed. A 2.5* 1.8 * 0.6 cm mass, clinged with stalk from the right hepatic duct was noted and could be removecl by segmcntal resection of the common bile duct and the common hepatic duct. On cut section, greenisherous fluid containing multilocular cysts surrounded by a smooth wall with 0.3 cm thickness was seen. Microscopic findings revealed a cystadenoma lined by a single layer of columnar epitheliuni containing intracellular mucin pools without evidence of cellular atypism or mitosis.

HBsAg 과 Anti - HBs 가 동시 양성예의 임상적 경과 관찰

박효진(Hyo Jin Park),김경희(Kyung Hee Kim),노형근(Hyung Keun Roh),박종훈(Chong Hoon Park),이관식(Kwan Sik Lee),문영명(Young Mung Moon),강진경(Jin Kyung Kang),최흥재(Heung Jai Choi),송경순(Kyung Soon Song) 대한내과학회 1990 대한내과학회지 Vol.39 No.1

N/A To assess the clinical implication of concomitant HBsAg and anti-HBs in patients with HBV infection, we studied the longitudinal behavior of HBsAg, anti-HBs and their subtypes and the correlation of clinical events in 25 patients with concomitant HBsAg and anti-HBs. The results were as follows: 1) Concurrent markers were persistent in 12 (57.1%) out of 21 patients with chronic liver disease. In two patients, anti-HRs was detected intermittently during the follow-up period. In each, the recurrent antibody was persistently heterotypic, and the appearance, disappearance and reappearance of anti-HBs were not associated with alterations in the clinical course. 2) The mean level of anti-HBs was 16.6±17.7 SN ratrio and 61.9% of the patients were less than 10 SN ratio. 3) In the majority of patients, HBsAg subtype ad was accompanied by anti-y. In addition, in two patients with intermittent concomitance the recurrent amtibody was persistently heterotypic. 4) During the follow-up period, acute exacerbations of the disease occurred in four out of 21 patients with chronic hepatitis. All patients were initially HBsAg subtype ad and remained so during and after exacerbation. Therefore, the possibility of reinfection with HBV of a different subtype was not supported. In conclusion, concomitant HBsAg and anti-HBs are not uncommon in patients with acute and chronic hepatitis B and do not reflect changes in the clinical courses. The concomitance is of no practical or prognostic significance, and therefore, the attention devoted to it may be clinically unnecessary.

각종 간질환 환자에서 혈청 Type IV Collagen 측정

강진경(Jin Kyung Kang),최흥재(Heung Jai Choi),박인서(In Suh Park),문영명(Young Myung Moon),한광협(Kwang Hyub Han),전재윤(Chae Yoon Chon),박찬일(Chan Il Park),박영년(Young Nyun Park),정진이(Jin Yi Chung) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.2

N/A Serum type IV collagen levels determined with one-step sandwich enzyme immunoassay using monoclonal antibodies were compared with histologic changes in the liver biopsy specimens from 95 patients with various liver diseases. Although serum type IV collagen levels were not significantly increased in patients with fatty liver and chronic persistent hepatitis compared to normal controls .serum type IV collagen levels were significantly increased in patients with chronic liver disease such as chronic active hepatitis or liver rirrhosis compared to normal controls and patients with fatty liver and chronic persistent hepatitis. According to the progression of liver disease, serum type IV collager levels wen significantly increased. In addition, all the 31 patients with chronic active hepatitis with early cirrhosis and liver cirrhosis showed elevated serum type IV collagen levels higher than mear plus one standard deviation level of controls. Serurn type. IV collagen levels did not correlate with serum transaminase levels in patients with chronic liver disease, A significant positive correlation was found between the serum type IV collagen level and the degree of fibrosis, piecemeal necrosis and portal and/or periportal inflamniation in patients with chronic active hepatitis. the results suggest that the serum type IV collagen levels correlate well with the degree of hepatir fibrosis. Therefore the measurement. of serum type IV collagen level is relatively simple and useful method to reflect the progress of hepatic fibrosis.

Trimethyltin 유도성 인지기능 저하 동물 모델에 대한 들기름의 개선효과

강진용(Jin Yong Kang),박보경(Bo Kyeong Park),승태완(Tae Wan Seung),박창현(Chang Hyeon Park),박선경(Seon Kyeong Park),진동은(Dong Eun Jin),강성원(Sung Won Kang),최성길(Sung-Gil Choi),허호진(Ho Jin Heo) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.3

본 연구에서는 들기름의 TMT 유도성 인지 기능 상실에 대한 개선 효과와 함께 가정에서 식용유로서 사용량이 많은 대두유와 그 효과를 비교·연구하였다. 실험에서 들기름과 대두유를 섭취한 마우스를 TMT로 인지 기능 손상을 유발하여 Y-maze test와 Morris water maze test 한 결과, 공간 인지 기능 및 학습능력 개선에 대해 대두유는 효과가 미비하였으나 들기름은 대조군과 유의적인 차이가 없는 정도의 유의적 개선 효과를 보였다. 동물 실험 후 mouse로부터 적출된 뇌 조직을 대상으로 AChE 활성, MDA 함량, SOD 활성 측정 및 산화된 glutathione 측정한 결과, 대두유는 TMT 단독 처리군의 경우와 유사한 반면 들기름은 TMT에 의해 손상을 입은 mice의 뇌 조직에서 AChE의 활성과 MDA 생성 및 GSH의 산화를 억제시킬 뿐만 아니라 SOD 활성을 유의성 있게 증가시킨 것으로 나타났다. 이러한 결과는 들기름에 존재하는 풍부한 생리 활성 물질로서 ω-3계 지방산, 페놀화합물 그리고 비타민 E 등에 의한 것으로 사료된다. 결국 들기름은 상대적으로 우수한 인지 기능 개선 효과를 나타냈고, 이는 건강기능식품으로서 고부가가치 소재로 활용될 수 있는 산업적 활용 가능성을 나타내는 것으로 판단된다. This study aimed to investigate the anti-amnesic effect of perilla oil against trimethyltin (TMT)-induced learning and memory impairment in ICR mice. Perilla oil (2.5 mL/㎏ of body weight) and soybean oil (2.5 mL/㎏ of body weight) were administered orally to mice for 3 weeks, and at the end of the experimental period, cognitive behavior was examined by Y-maze and Morris water maze (MWM) tests. Behavioral tests showed that the mice treated with perilla oil had improved cognitive function compared to that in mice administered soybean oil. Analysis of brain tissue showed that perilla oil significantly lowered acetylcholinesterase activity and malondialdehyde (MDA) levels. Oxidized glutathione (GSH)-to-total GSH ratio also decreased from 10.4% to 5.3% in perilla oil-treated mice, but superoxide dismutase (SOD) activity increased from 11.7 to 14.2 U/㎎ protein. Therefore, these results suggest that the perilla oil could be a potential functional substance for improving cognitive function.

간경변증환자의 HLA 항원 분포에 관한 연구

강진경 ( Jin Kyung Kang ),박인서 ( In Suh Park ),최흥재 ( Heung Jai Choi ),문영명 ( Young Myoung Moon ),이상인 ( Sang In Lee ),김영수 ( Young Soo Kim ),박계숙 ( Kye Sook Park ),박기일 ( Ki Il Park ) 대한내과학회 1983 대한내과학회지 Vol.26 No.11

활성 산소종으로 야기된 산화스트레스에 대한 와송 추출물의 신경세포 보호효과 및 주요 생리활성물질

박수빈(Su Bin Park),이두상(Du Sang Lee),강진용(Jin Yong Kang),김종민(Jong Min Kim),박선경(Seon Kyeong Park),강정은(Jeong Eun Kang),권봉석(Bong Seok Kwon),박상현(Sang Hyun Park),이창준(Chang Jun Lee),이욱(Uk Lee),허호진(Ho Jin Heo) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.5

본 연구에서는 와송(Orostachys japonicus A. Berger)의 산화방지 효과와 산화스트레스로부터의 신경세포 손상에 대한 보호효과 및 주요 생리활성물질을 분석하였다. 와송 아세트산에틸 분획물(ethylacetate fraction from Orostachys japonicus A. Berger extract:EFOJ)은 다른 분획물보다 높은 총 페놀화합물 함량을 나타냈으며, 와송 아세트산에틸 분획물(EFOJ)로 ABTS, DPPH 라디칼 소거 활성과 FRAP 검정 및 지방질과산화물 억제력 분석을 실시한 결과, 우수한 라디칼 소거 활성, 총산화방지력과 과산화 지방질 생성물의 억제력을 확인하였다. 또한 와송 아세트산에틸 분획물(EFOJ)은 과산화수소로 인한 신경세포 사멸에 대한 세포 생존율을 향상시켰으며, 신경 세포막 손상을 보호하는 능력이 우수한 것을 확인하였다. 이러한 생리 활성을 가진 와송 아세트산에틸 분획물(EFOJ)의 주요물질을 분석하기 위하여 LC-MSe를 실시하였으며, 주요 생리활성 물질은 퀘세틴 배당체와 캠페롤 배당체로 추정되었다. 본 연구 결과들을 바탕으로, 와송 추출물은 우수한 산화방지력을 가지고 있을 뿐만 아니라 산화적 스트레스로부터의 신경세포 보호효과를 통해 퇴행성 신경질환과 같은 질병을 예방 할 수 있는 건강기능성식품의 천연산화방지제 소재로써 고부가 가치가 있다고 판단된다. The study aimed to investigate the antioxidant activity and neuroprotective effect of the ethyl acetate fraction from Orostachys japonicus A. Berger extract (EFOJ) and its main constituent compounds. Among all fractions, the highest content of total phenolics was found in EFOJ. The antioxidant activity of EFOJ was confirmed through the 2,2-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid (ABTS), 1-1-diphenyl-2-picryl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP) assays and the inhibitory effect of malondialdehyde (MDA). In addition, we ascertained that EFOJ not only decreased the intracellular ROS level, but also protected the neuronal cells against H2O2-induced oxidative stress. In liquid chromatography-mass spectrometry analysis, the following were found to be the main compounds of EFOJ: quercetin-3- O-glucoside, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside, and kaempferol-3-O-rhamnoside. Consequently, these results suggested that the protective effect on neuronal cells was based on the antioxidant activities of the physiologically active compounds of Orostachys japonicus A. Berger extract, which could therefore help to mitigate neurodegenerative diseases.

간장및 담도 : 간경변증 및 간세포암에 있어서 Doppler 초음파검사로 측정한 복강내 동맥의 혈류속도파형에 관한 연구

강진경(Jin Kyung Kang),최흥재(Heung Jai Choi),박인서(In Suh Park),김원호(Won Ho Kim),송시영(Si Young Song),박형석(Hyung Seok Park) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.2

N/A The duplex scanner combines a pulsed Doppler flowmeter with a high resolution real-time scanner, so that blood flow information can be collected from a selected position in a specific vessel, using the real-time capability of the system. Several studies on the changes of blood flow velocity waveform were reported according to the hypothesis that every artery has its own characteristic Doppler-shift signal and that this is modified by disease, either of the artery itself or of the tissue fed by the vessel. To recognize the normal value of the systolic/diastolic velocity ratio and resistance index and their changes in liver cirrhosis and hepatocellular carcinoma, we analysed the blood flow veIocity waveform of the splanchnic arteries detected by Doppler ultrasonography in 22 healthy subjects as controls, 23 patients with liver cirrhosis and 38 patients with hepatocellular carcinoma. The results obtained were as follows: 1) In controls, the S/D ratio and resistance index of the hepatic artery were 4.37 +- 1.87 and 0.74 +- 0.08, respectively; the S/D ratio and resistance index of the splenic artery were 3.45 +- 0.73 and 0.70 +- 0.06, respectiveiy; and the S/D ratio and resistance index of the superior mesenteric artery were 6.99 +- 1.42 and 0.85 +- 0.03, respectively. 2) The S/D ratios and resistance indices of the hepatic artery and splenic artery of the patients with liver cirrhosis or hepatocellular carcinoma were not different compared with those of controls. However, the S/D ratio and resistance index of the superior mesenteric artery of the patients with liver cirrhosis or hepatocellular carcinoma were significantIy smaller than those of controls. 3) In patients with hepatocellular carcinoma, the larger the tumor and the more severe the arterio-venous anstomosis, the smaller the S/D ratio and resistance index of the hepatic artery. These results suggest that every splanchnic artery has its own characteristic blood flow velocity waveform, and the peripheral resistance of the superior mesenteric artery decreases in patients with liver cirrhosis or hepatocellular carcinoma.

초미세먼지(PM<SUB>2.5</SUB>)로 유도된 in vitro 세포 독성에 대한 감태(Ecklonia cava) 추출물의 보호 효과

박선경(Seon Kyeong Park),강진용(Jin Yong Kang),김종민(Jong Min Kim),유슬기(Seul Ki Yoo),한혜주(Hye Ju Han),신은진(Eun Jin Shin),허호진(Ho Jin Heo) 한국식품과학회 2019 한국식품과학회지 Vol.51 No.5

감태(Ecklonia cava) 추출물의 in vitro 항산화효과 및 초미세먼지로 유도된 세포 독성에 대한 비강(RPMI-2650), 폐(A549) 및 뇌신경(MC-IXC) 세포에서의 세포보호효과를 검증하였다. 감태는 총 페놀성화합물(물 추출물: 189.32, 80% 에탄올 추출물: 418.08 mg GAE/g of dried weight) 및 총 플라보노이드(물 추출물: 44.93, 80% 에탄올추출물 111.22mg RE/g) 함유량을 각각 나타내었으며, ABTS/DPPH 라디칼 소거활성 및 지방질과산화 억제효과에서도 우수한 효과를 나타냈다. 또한, 감태 추출물은 초미세먼지로 유도될 수 있는 세포 내 산화적스트레스를 저해함으로써 세포 사멸을 효과적으로 억제시킬 수 있는 천연 소재로 판단되며, 80% 에탄올 추출물뿐만 아니라 물 추출물 또한 산업적 활용가능성이 기대된다. To evaluate the protective effect of Ecklonia cava on ultra-fine dust (PM2.5)-induced cytotoxicity, we investigated the in vitro antioxidant activity and cell viability after exposure to PM<SUB>2.5</SUB>. E. cava was extracted using water and 80% ethanol, and antioxidant activity was determined using the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)/2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation inhibition assays. The 80% ethanol extract showed relatively higher antioxidant activity than the water extract. The cell protective effects were determined by measuring the intracellular reactive oxygen species (ROS) content and viability of nasal epithelial (RPMI-2650), lung epithelial (A549), and brain neuroblastoma (MC-IXC) cells. Results showed that the 80% ethanol extract inhibited ROS production more than the water extract. In contrast, both extracts showed similar effects on cell viability in the PM<SUB>2.5</SUB>-induced cell death assay. Thus, Ecklonia cava may act as an effective resource for preventing PM<SUB>2.5</SUB>-induced cytotoxicity in nasal, lung, and brain cells.