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Sinapine 누출정도에 따른 무 ( Raphanus sativus L . ) 종자의 발아율과 종자 및 유묘의 외형적 차이
민태기,김복진,백준호 ( Tai Gi Min,Bok Jin Kim,Jun Ho Back ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.3
Seeds of five radish varieties were soaked in water for three hours and cellulose was coated. The seeds were classified as three groups in UV light; non-fluorescent(NF), partly fluorescent(PF), and fully fluorescent(FF) seeds. Germination rate was less in the order of NF$gt;PF$gt;FF seeds. The seed coat structure of NF seeds was dense and showed round shape, while those of PF and FF seeds were wrinkled or ruptured. The cotyledon and hypocotyl of NF seeds were normal, while those of PF and FF seeds were dwarf and showed some scars in the cotyledons.
Sinapine 누출정도에 따른 무(Raphanus sativus L.) 종자의 발아율과 종자 및 유묘의 외형적 차이
민태기,백준호,김복진,Min, Tai-Gi,Back, Jun-Ho,Kim, Bok-Jin 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.2
5개 무 품종의 종자를 3시간 물에 침지한 후 cellulose 분말을 코팅하여 UV 광하에서 sinapine 누출정도에 따른 형광색의 분포정도에 따라 무형광(NF)종자, 일부형광(PF)종자, 완전형광(FF)종자로 구분하고, 또 이들 종자와 발아한 유묘의 외부형태를 주사전자현미경(SEM), 광학현미경 및 육안으로 관찰하였다. 종자의 발아율은 무형광(NF)종자>일부형광(PF)종자>완전형광(FF)종자 순으로 낮았으며, 무형광(NF)종자는 종피조직이 치밀하고 둥근 모양이었으나, 일부형광(PF) 및 완전형광(FF)종자는 종피가 주름지거나 상처가 있는 것이 대부분이었다. 또 무형광(NF)종자에서 나온 유묘의 떡잎과 배축은 정상이었으나, 일부형광(PF) 및 완전형광(FF)종자에서 나온 유묘의 떡잎에는 조직이 괴사한 흔적이 많았으며 떡잎과 배축의 크기가 작고 비정상적이었다. Seeds of five radish varieties were soaked in water for three hours and cellulose was coated. The seeds were classified as three groups in UV light; non-fluorescent(NF), partly fluorescent(PF), and fully fluorescent(FF) seeds. Germination rate was less in the order of NF>PF>FF seeds. The seed coat structure of NF seeds was dense and showed round shape, while those of PF and FF seeds were wrinkled or ruptured. The cotyledon and hypocotyl of NF seeds were normal, while those of PF and FF seeds were dwarf and showed some scars in the cotyledons.