폐색전증 환자에서 발견된 Factor VII 유전자의 프로모터 -401G/A 다형성 1예

민보람,김신,박지혜,채진녕,최원일,Min, Bo Ram,Kim, Shin,Park, Ji Hae,Chae, Jin Nyeong,Choi, Won Il 대한결핵및호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.6

저자들은 저자들은 폐색전증이 발생한 환자에서 기저질환을 조사하던 중 Factor VII 활성도 저하를 관찰하였고, 이 환자에게서 Factor VII 유전자의 프로모터 -401의 단염기 치환($G{\rightarrow}A$)을 발견하여 보고하며, 국내에도 Factor VII 유전자 다형성이 존재함을 밝히며, Factor VII 활성도 감소와 폐색전증이 동반된 환자를 보고하는 바이다. A factor VII gene -401 G/A polymorphism was identified in a patient with a pulmonary embolism. The patient was a 71-year-old woman who presented with acute-onset dyspnea. A chest CT scan revealed a pulmonary embolism. Despite the administration of low-dose warfarin as anticoagulation therapy, there was an excessively prolonged prothrombin time (PT). The blood tests revealed lower factor VII activity than normal. Full factor VII gene sequencing revealed a G to A substitution at -401 in the promoter region. There were no other gene sequence anomalies. PCR-based analysis indicated lower factor VII gene expression in the patient than in a control subject. The data suggested the promoter polymorphism to be responsible for the lower transcription level. In conclusion, we encountered a case of Factor VII DNA polymorphism in a patient with a pulmonary embolism showing significantly reduced Factor VII activity.

황색포도알균의 감염에 따른 세포 내에서의 균의 증식과 Matrix Metalloproteinase (MMP)의 역할

민보람 ( Bo Ram Min ),이영미 ( Young Mi Lee ),박재석 ( Jae Seok Park ),최원일 ( Won Il Choi ),권건영 ( Kun Young Kwon ) 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.1

연구배경: 본 연구에서는 황색포도알균이 숙주세포 내로 침입하여 증식하는 정도를 관찰하고자 한다. 이때 세포 외 바탕 단백질의 변화가 수반될 것으로 가설을 설정하고, 이러한 변화에 영향을 미칠 것으로 생각되는 matrix metalloproteinase (MMP)의 발현과 역할에 대해 연구하고자 하였다. 방 법: 황색포도알균은 106∼107 CFU/ml을 105개의 기관지상피세포인 BEAS-2B 세포에 2시간 동안 침입시킨다. 이후 세척으로 세포 밖에 있는 황색포도알균을 제거한 후, BEAS-2B 세포를 다양한 시간 동안(4, 6, 8, 12 시간) 배양한 후 황색포도알균의 집락수(CFU/ml)를 측정하였고, 단백질을 분리하여 세포 외 바탕단백질의 발현 정도와 MMP의 활성도를 측정하였다. 또한 MMP 억제제인 GM6001을 전처치한 후 황색포도알균을 세포에 침입시킨 후 세포 내 에서의 균의 집락수 및 세포 외 바탕단백질의 변화를 관찰 하였다. 결 과: 황색포도알균의 집락을 측정한 결과 4시간과 12시간을 비교해 볼 때 MOI가 증가할수록, 감염시킨 시간이 길수록 숙주세포 내로 침입이 유의하게 증가하였다. BEAS-2B 세포에서 황색포도알균을 침입시킨 시간이 길수록, MOI가 증가할수록 MMP 2 및 MMP 9의 활성도와 dysadherin의 발현은 증가하였고, 이와는 대조적으로 E-cadherin의 발현은 감소하였다. MMP억제제인 GM6001을 전 처치 한 결과 황색포도알균의 세포 내 침입을 유의하게 감소시켰다. 결 론: 황색포도알균이 기관지 상피세포 내로 침입할 때 dysadherin 및 E-cadherin 같은 세포 외 바탕 단백질의 변화를 동반하며, MMP 활성도가 균의 세포 내 침입에 관여하는 것으로 보인다. Background: Staphylococcus aureus frequently colonizes and infects hospitalized patients. Respiratory infections with Staphylococcus aureus are common in patients with compromised airway defenses. However the mechanisms of S. aureus invasion from colonization to the epithelium are unclear. Cell invasion by S. aureus would require destruction of the extracellular matrix, which is believed to be the result of increased matrix metalloproteinases (MMP) activity. Methods: In this study, respiratory epithelial cells were infected with S. aureus. After removing the extracellular bacteria by washing, the internalized bacteria in the cells were assessed by counting the colonized forming units (CFUs). The cell adhesion proteins, dysadherin and E-cadherin, were evaluated by Western blotting. The MMPs in the bacterial invasion were evaluated by pretreating the cells with GM6001, a MMP inhibitor. Results: The internalization of S. aureus was found to be both time and dose dependent, and the increase in MMP 2 and 9 activity was also dependent on the incubation time and the initial amount of bacterial inoculation. The invasion of S. aureus was attenuated by GM6001 after 12 hours incubation with a multiply of infection (MOI)=50. The expression of dysadherin, a membrane protein, was increased in a time and dose dependent manner, while the expression of E-cadherin was decreased. Conclusion: MMPs may mediate the invasion of S. aureus into epithelial cells. (Tuberc Respir Dis 2008;64:22-27)

폐색전증 환자에서 발견된 Factor 7 유전자의 프로모터 -401G/A 다형성

민보람 ( Bo Ram Min ),김신 ( Shin Kim ),박지혜 ( Ji Hae Park ),채진녕 ( Jin Nyeong Chae ),최원일 ( Won Il Choi ) 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.6

A factor VII gene -401 G/A polymorphism was identified in a patient with a pulmonary embolism. The patient was a 71-year-old woman who presented with acute-onset dyspnea. A chest CT scan revealed a pulmonary embolism. Despite the administration of low-dose warfarin as anticoagulation therapy, there was an excessively prolonged prothrombin time (PT). The blood tests revealed lower factor VII activity than normal. Full factor VII gene sequencing revealed a G to A substitution at -401 in the promoter region. There were no other gene sequence anomalies. PCR-based analysis indicated lower factor VII gene expression in the patient than in a control subject. The data suggested the promoter polymorphism to be responsible for the lower transcription level. In conclusion, we encountered a case of Factor VII DNA polymorphism in a patient with a pulmonary embolism showing significantly reduced Factor VII activity.

원발성 간혈관육종의 임상적 고찰: 8예 분석

허창재 ( Chang Jae Hur ),민보람 ( Bo Ram Min ),이유진 ( Yoo Jin Lee ),장병국 ( Byung Kuk Jang ),황재석 ( Jae Seok Hwang ),김은수 ( Eun Soo Kim ),박경식 ( Kyung Sik Park ),조광범 ( Kwang Bum Cho ),강유나 ( Yu Na Kang ),정우진 ( Woo 대한소화기학회 2015 대한소화기학회지 Vol.65 No.4

Background/Aims: Hepatic angiosarcoma, a rare and aggressive liver malignancy, is difficult to diagnose because of a lack of specific clinical features. The clinical and radiological features of patients with histologically confirmed hepatic angiosarcoma were examined. Methods: Among 2,336 patients diagnosed with primary hepatic carcinoma at Keimyung University Dongsan Medical Center (Daegu, Korea) between May 2002 and February 2012, eight (0.03%) with histologically confirmed primary hepatic angiosarcoma were included. The patterns of disease diagnosis, tumor characteristics, treatment responses, and prognoses were reviewed retrospectively. Results: Median age was 66 years-old (range, 41-80 years). Four patients were male. Five patients were compulsive drinkers. All patients had no HBsAg and anti-HCV. Initial radiologic diagnoses revealed primary hepatic angiosarcoma (n=2), hepatocellular carcinoma (n=2), hemangioma (n=2), and hepatic metastatic carcinoma (n=2). Definitive diagnoses were made by percutaneous needle biopsies in seven patients and surgical resection in one patient. At the time of the initial diagnosis, extrahepatic metastases were detected in three patients (37.5%). Metastatic sites included the spleen and lung, pericardium, and bone, in one patient each. Two patients underwent conservative treatments. The remaining patients underwent surgical resection (n=1), transcatheter arterial chemoembolization (n=1), and systemic chemotherapy (n=4). The median survival period was 214 days (range, 21-431 days). Conclusions: Hepatic angiosarcoma is a highly progressive disease with a poor prognosis. Detailed studies including histological examinations are essential to facilitate early diagnosis of the disease. (Korean J Gastroenterol 2015;65:229-235)

식품안전성 확보와 식품산업 발전

이병오 ( Byung Oh Lee ),민보람 ( Bo Ram Min ),김성철 ( Cheng Zhe Jin ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2009 강원 농업생명환경연구 Vol.20 No.-

Food safety is the most important factor to develop the food industry. Korean criteria of food safety should be increased to the international level to export agro-food products. But the financial supports and shift period will be necessary for small size producers. Now the level of Korean food system is on the developing stage. And the confidence level for food safety policies of government is not so high. To improve the confidence of consumers and to construct the strong food safety system, many measures should be adopted together. Risk Analysis System, Precautional Principal, and Crisis Management are basic frames and principles for food safety. HACCP is very effective measure to insure food safety on the physical view point. On the other hand, risk communication and traceability system are also good measures to increase consumers` confidence on the mental view point. Food companies can improve food safety by taking up the Compliance and ISO standards. The roles and responsibilities of mass media for food safety are very important.

만성폐쇄성폐질환 환자의 호흡곤란 평가에서 우심실 박출계수의 의의

이정은 ( Jung Eun Lee ),민보람 ( Bo Ram Min ),박재석 ( Jae Seok Park ),박훈표 ( Hun Pyo Park ),전미정 ( Mi Jung Jun ),원경숙 ( Kyung Sook Won ),최원일 ( Won Il Choi ) 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.60 No.6

배경: 만성폐쇄성폐질환 환자의 호흡곤란은 일반적으로 폐활량에 반비례하나 유사한 폐기능에서도 서로 다른 호흡곤란을 호소한다. 본 연구는 만성폐쇄성폐질환 환자에서 우심실박출계수와 호흡곤란의 정도와 연관관계가 있는지를 알아보고자 한다. 방법: 호흡곤란의 정도는 Medical Research Council (MRC) 호흡곤란척도로 분석하였고, MRC 4/5도인 중증군 24명과, MRC 2/3도인 중등증군 16명을 비교 분석하였다. 심전도게이트 일회통과법을 이용한 방사성동위원소 심조영술을 이용하여 우심실 박출계수를 구했으며, 안정시 동맥가스분석 및 폐기능검사를 시행하였다. 결과: 기저 폐기능에서 잔기량의 예측치 평균이(%) 중증군에서(210±87) 중등증군(160±27)에 비해 유의하게 증가되었으나(P<0.03), 폐활량 및 확산계수 등에서는 유의한 차이가 관찰되지 않았다. 우심실 박출계수(%)는 중증군에서(25±8) 중등증군(35±6)에 비해 유의하게 감소되었으나(P<0.001), 동맥혈가스는 두 군 사이에 유의한 차이가 관찰되지 않았다. 다중회귀분석을 통해 우심실 박출계수가 독립적으로 호흡곤란에 영향을 미치는 인자로 밝혀졌다. 결론: 만성폐쇄성폐질환에서 우심실 박출계수가 호흡곤란의 정도에 영향을 미치는 것으로 보인다. Background: Patients with COPD generally complain of very different degrees of dyspnea regardless of their pulmonary function. The study, we assessed the right ventricular ejection fraction in relation to dyspnea in COPD patient. Methods: The pulmonary function including the diffusion capacity was measured. The right ventricle ejection fraction (RVEF) was measured using a first-pass radionuclide scan by multigated acquisition (MUGA). Forty patients with chronic obstructive pulmonary disease (COPD) were stratified for dyspnea according to the Medical Research Council (MRC) scale. Moderate dyspnea and severe dyspnea is defined as MRC 2/3 (n=16) and MRC 4/5 (n=24) respectively. Results: The baseline pulmonary function tests including DLCO and the resting arterial blood gas were similar in the moderate and severe dyspnea group, with the exception of the residual volume (% predicted) (moderate 160±27, severe 210±87, p<0.03). The right ventricle ejection fraction was significantly (p<0.001) lower in the severe dyspnea group (25±8) than in the moderate group (35±6). The independent factor assessed by multiple logistic regression revealed only the severity of dyspnea to be significantly associated with RVEF (p<0.02). Conclusion: This study showed that the right ventricle ejection fraction would contributes to severity of dyspnea in patients with a similar pulmonary function (Tuberc Respir Dis 2006; 60: 631-637)

폐색전증이 의심된 환자에서 두 가지 폐색전증 진단 예측 모형의 평가

박재석 ( Jae Seok Park ),최원일 ( Won Il Choi ),민보람 ( Bo Ram Min ),박지혜 ( Jie Hae Park ),채진녕 ( Jin Nyeong Chae ),전영준 ( Young June Jeon ),유호정 ( Ho Jung Yu ),김지영 ( Ji Young Kim ),김경주 ( Gyoung Ju Kim ),고성민 ( Su 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.4

패널데이터를 이용한 국내산 쇠고기 구매 영향요인 분석

윤진우 ( Yun Jin-woo ),최현호 ( Chio Hyun-ho ),민보람 ( Min Bo-ram ),최종산 ( Choi Jong-san ) 韓國食品流通學會 2017 한국식품유통학회 학술대회 Vol.- No.하계