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허민수(Min Soo Heu),박창경(Chang Kyoung Park),지성준(Seung Joon Jee),민관희(Kwan Hee Min),김민정(Min Jung Kim),김은정(Eun Jung Kim),강경태(Kyung Tae Kang),김진수(Jin?Soo Kim) 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.1
남해안 일대에서 다량 생산되고 있는 굴의 소비 촉진을 위하여 조미 굴포의 제조를 시도하였다. 굴 첨가비율에 따른 조미 굴포의 일반성분, 색조 및 관능검사의 결과로 미루어 보아 조미 굴포의 제조를 위한 굴의 최적 첨가비율은 30%로 판단되었다. 최적 조건에서 제조한 조미 굴포는 조미 수리미포에 비하여 아미노산의 총함량은 차이가 없었으며 주요 아미노산으로는 glutamic acid, leucine, lysine 및 threonine등이었다. 또한, 조미 굴포의 칼슘 및 인 함량은 조미 수리미포에 비해 높았으며, 조미 굴포의 유리아미노산의 총함량 및 taste value는 각각 1,576.8 ㎎/100 g 및 226.05이었고 맛의 지대하게 영향을 미치는 아미노산은 glutamic acid 및 aspartic acid이었다. New type of seasoned and dried oyster slice (SDOS) was prepared with surimi and oyster, and then characterized on the food components. With a higher oyster content ratio, the moisture (18.1% to 21.7%), water activity (0.621 to 0.661), and ΔE value (74.83 to 75.90) of SDOS slightly increased. Regardless of differences in oyster content ratios, there was, however, no difference in the sensory color and flavor expect for sensory texture of SDOS. The desirable ratio of oyster content for preparing the seasoned and dried oyster slice was determined as 30% according to the results above. There was no difference in total amino acid content between seasoned and dried slices with and without oyster. The major amino acids of SDOS were glutamic acid, leucine, lysine and threonine. The calcium and phosphorus contents of SDOS were 64.4 ㎎/100 g and 315.9 ㎎/100 g, respectively. The total free amino acid content and taste value of SDOS were 1,576.8 ㎎/100 g and 226.05, respectively. The results suggested that SDOS could be used as jerky?like oyster products.
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노경희(Noh, Kyung-Hee),민관희(Min, Kwan-Hee),서보영(Seo, Bo-Young),김소희(Kim, So-Hee),서영완(Seo, Young-Wan),송영선(Song, Young-Sun) 한국영양학회 2012 Journal of Nutrition and Health Vol.45 No.5
This study was performed to examine the characteristics of protein of red crab (Chionoecetes japonicus) shell powder hydrolyzed by commercial proteases. Red crab shell was digested by commercial proteases, such as Protamex (P), Neutrase (N), Flavourzyme (F), Alcalase (A), Protease M (PM) and Protease A (PA). Protein yield analyzed by Biuret assay, absorbance at 280 nm and brix revealed that PA was the enzyme having the highest proteolytic activity. SDS PAGE showed that molecular weight of proteins produced by protease treatments was various and below 150 kDa. Combinational treatment of proteases (PA + P, PA + PM, PA + F, PA + A) was tried whether these increase protein hydrolysis from red crab shell powder compared to a PA single treatment. Soluble protein content was similar, but amino acid concentration by combinational treatments was higher than PA single treatment [PA + P 247.4 mg/g > PA + F (206.4 mg/g) > PA + A (133.4 mg/g) > PA + PM (59.1 mg/g) > PA (54.9 mg/g)]. Amino acid composition by combinational treatments was slightly different. Most abundant essential amino acids were phenylalanine, glycine, alanine, and leucine, whereas tyrosine and cystine were not detected. (Korean J Nutr 2012; 45(5): 429 ~ 436)
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RAW264.7 Macrophage Cell에서 녹차씨껍질 에틸아세테이트 분획의 염증억제 효과 및 기전 연구
노경희(Kyung-Hee Noh),장지현(Ji-Hyun Jang),민관희(Kwan-Hee Min),친조리그 라드나바자르(Radnaabazar Chinzorig),이미옥(Mi-Ock Lee),송영선(Young-Sun Song) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.5
본 연구는 녹차씨껍질 분획 추출물 중 염증 저해능이 강력한 EtOAC분획을 선정하여 대식세포주인 RAW264.7 macrophage cell에서 항염증효과의 기전을 생화학적, 분자학적 수준에서 분석하고자 하였다. 녹차씨껍질 추출물 100 ㎎당 총 페놀함량은 EtOAC분획에서 가장 높은 수준이었으며 EtOH추출물>PE분획>BuOH분획과 H₂O분획의 순으로 나타났다. 또한 EtOAC분획의 NO 억제능이 가장 강한 것으로 나타나, EtOAC분획의 polyphenol을 분석한 결과 EGC(1146.5±11.01 ㎍/g)> tannic acid(967.0±32.24 ㎍/g)> EC(70.9±4.39 ㎍/g)> gallic acid(947.6±1.03 ㎍/g)> caffeic acid(37.7±1.46 ㎍/g)> ECG(35.5±3.19 ㎍/g)> EGCG(15.5±0.09 ㎍/g)의 순으로 나타났다. 녹차씨껍질 EtOAC분획이 RAW264.7 macrophage cell에서 LPS 처리에 의한 산화적 스트레스로 발생되는 NO 생성을 농도 의존적으로 감소시키며(IC50: 80.11 ㎍/mL) PGE₂의 생성을 억제하였다. 염증생성 전사인자인 iNOS의 유전자 발현은 농도 의존적으로 억제시켰으나 COX-2의 단백질 발현에는 영향을 미치지 않았다. 녹차씨껍질 EtOAC분획은 총 항산화능과 GSH 수준을 증가시켜 산화적 스트레스를 경감시키는 역할을 하며 항산화효소계인 catalase, GSH-red 및 Mn-SOD 활성의 단백질 발현을 증가시키는 것으로 나타났다. 핵에서의 p65 농도는 대조군에 비해 녹차씨껍질 EtOAC분획을 처리한 군에서 현저하게 낮은 것으로 나타났다. 이상의 결과에서, 녹차씨껍질 EtOAC분획은 NF-κB 활성을 억제함으로써 iNOS 단백질 발현을 억제하여 NO의 생성을 감소시키고 총 항산화능과 GSH 수준을 증가시키며, 항산화 효소계를 활성화시켜 세포내 산화적 스트레스를 감소시킴으로써 LPS 자극에 의한 염증반응을 지연하거나 억제하는 것으로 사료된다. Green tea seed coat (GTSC) was extracted with 100% ethanol for 4 hr and then fractionated with petroleum ether (PE), ethyl acetate (EtOAC) and butanol (BuOH). The EtOAC fraction showed the highest level in total phenol contents and the lowest level in nitric oxide (NO) production in LPS-stimulated RAW264.7 macrophage cell. Thus, this study was carried out to investigate the anti-inflammatory and its mechanisms of GTSC EtOAC fraction in LPS-stimulated RAW264.7 macrophage cell. GTSC EtOAC fraction contained EGC (1146.48±11.01 ㎍/g), tannic acid (966.99±32.24 ㎍/g), EC (70.88±4.39 ㎍/g), gallic acid (947.61±1.03 ㎍/g), caffeic acid (37.69±1.46 ㎍/g), ECG (35.46±3.19 ㎍/g), and EGCG (15.53±0.09 ㎍/g) when analyzed by HPLC. NO production was significantly (p<0.05) suppressed in a dose-dependent manner with an IC50 of 80.11 ㎍/mL. Also prostaglandin E₂ level was also inhibited in a dose-dependent manner. Moreover, iNOS protein expression was suppressed in dose-dependent manner but COX-2 gene expression was not affected. Total antioxidant capacity and glutathione (GSH) levels were enhanced more than the LPS-control. Expressions of antioxidative enzymes including catalase, GSH-reductase and Mn-SOD were elevated compared to LPS-control. Nuclear p65 level was decreased in the GTSC EtOAC fraction in a dose-dependent manner. These results indicate that GTSC EtOAC fraction inhibit oxidative stress and inflammatory responses through elevated GSH levels, antioxidative enzymes expressions and suppression of iNOS expression via NF-κB down-regulation.
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