농생물게놈활용연구사업 원예작물 핵심집단 구축 현황

문중경(Jung-Kyung Moon),전희라(Hee-ra Jeon),정준휘(June-Hui Jeong) 한국원예학회 2019 한국원예학회 학술발표요지 Vol.2019 No.5

콩의 보리 활물피복(活物被覆, living mulch) 재배에 의한 잡초발생 및 토양유실의 감소

서종호,문중경,정건호,서민정,허석철,구자환,황정동,배현경,김상열,Seo, Jong-Ho,Moon, Jung-Kyung,Jung, Gun-Ho,Seo, Min-Jung,Heo, Seok-Chul,Gu, Ja-Hwan,Hwang, Chung-Dong,Bae, Hyeon-Kyung,Kim, Sang-Yeol 한국작물학회 2018 한국작물학회지 Vol.63 No.3

Reduction in the occurrence of weed and erosion of slope soil by living mulch barley simultaneously sown with soybean were investigated at the Upland Experimental Field in Suwon City in 2013 and 2014. The aboveground dry matter of 200-300, 100-200 kg/10a of living mulch barley was obtained at 40 and 60 after sowing, respectively. The occurrence of weeds was significantly reduced by barley living mulch. The early growth of soybean was reduced significantly because of the competition with barley living mulch, but the late growth of soybean after natural drying of barley living mulch was recovered considerably. The soybean grain yield with barley living mulch was similar to that of the normal soybean cultivation, showing no significant difference between no mulch and living mulch treatments. With respect to soil erosion on the slope soil, the amount of run-off water decreased by 20-50% and the amount of soil loss decreased by 70-90% by barley living mulch. The reduction in soil erosion by the use of plastic film + barley living mulch was similar to that of only barley living mulch. The barley living mulch in soybean production can be a promising cultivation technique as it reduces weed occurrence and soil erosion without grain yield reduction.

콩 추출물 투여가 흰쥐 혈청의 항산화효소 활성에 미치는 영향

윤홍태,문중경,박금룡,김용호,신미경,김용욱,Yun Hong-Tae,Moon Jung-Kyung,Park Keum-Yong,Kim Yang-Ho,Shin Mi-Kyung,Kim Yeung-Wook 한국작물학회 2005 Korean journal of crop science Vol.50 No.suppl1

본 시험은 콩 추출물의 흰쥐 투여에 따른 항산화 효소의 활성을 분석하고, 추출물의 원료인 콩 품종간, 추출물의 투여량 및 지방식이조성에 따른 효소활성도를 측정하였다. 시험결과를 요약하면 아래와 같다. 1. 콩추출물을 투여한 처리구에서 SOD와 GSHpx의 활성이 대조구에 비하여 높게 나타났으나, 통계적 유의성은 인정되지 않았다. 2. 지방식이 및 콜레스테롤을 흰쥐에 투여한 결과 콩추출물 섭취에 따른 항산화계 효소 SOD와 GSHpx의 활성은 검정콩 1호가 다원콩 및 황금콩에 비하여 높았다. 3. 지방식이조성 및 추출물의 투여량에 따른 항산화계 효소 활성도는 통계적으로 유의성이 인정되지 않았다. 4. 각 지방식이에 따른 콩추출물의 SOD와 GSHpx활성도에서 품종과 콩추출물 농도간에서는 상호작용효과가 인정되었다. 5. GSHpx 활성도에서 품종, 콩추출물 농도 및 지방식이간에서 상호작용효과가 인정되었다. This study was conducted to determine antioxidative enzyme activity in serum of rats as affected by soybean cultivar, extract feeding concentration and fat dietary. In all cultivars, activities of antioxidant enzymes in treatment of various fat compositions and cholesterol followed by feeding soy-extracts were higher than in nonfeeding control, however, no significant differences existed in statistically. The activities of SOD and GSHpx in serum of rats when fed with various fat dietary were higher in Geomjeongkong 1 than in Tawonkong and Hwangkeumkong. Enzyme activities of SOD and GSHpx with each fat compositions in treatment soy-extracts was no significant differences statistically. There was significant differences among cultivars and concentrations of soy-extracts in interaction effect. Also, the activities of GSHpx was significant differences among cultivars and concentrations of soy-extracts and fat composition in interaction effect.

팥나방(Matsumuraeses phaseoli)과 어리팥나방(M. falcana)의 종간 교잡과 접합후 생식격리

정진교,박창규,문중경,김은영,조점래,서보윤,Jung, Jin Kyo,Park, Chang-Gyu,Moon, Jung-Kyung,Kim, Eun Young,Cho, Jum Rae,Seo, Bo Yoon 한국응용곤충학회 2019 한국응용곤충학회지 Vol.58 No.4

동소동속종인 팥나방(Matsumuraeses phaseoli)과 어리팥나방(M. falcana)(나비목: 잎말이나방과) 사이에 교잡 가능성을 알아보기 위해서 실험실에서 인위적으로 두 종을 교잡시켰다. 두 종의 암수를 교차하여 교미시켰을 때, F1 잡종세대가 발생하였다. 두 잡종세대 집단을 집단 내 및 집단 간 암수를 교차하여 교미시킨 경우들에서, 팥나방 암컷과 어리팥나방 수컷이 교잡되어 생성된 F1 잡종세대 집단(H집단)의 암컷이 같은 집단의 수컷 혹은 다른 집단의 수컷과 교미되었을 때, F2 후대를 거의 생성하지 못했다. 다른 집단으로 만들어진 F2 세대는 집단 내 교미에서 F3 세대를 생성시켰다. F1 잡종세대 집단 암수와 팥나방 혹은 어리팥나방 암수를 각각 교차하여 교미시킨 역교잡 8개 집단 중에서도 H집단의 암컷과 짝지어진 어미세대 수컷 집단 2개는 전혀 산란하지 못했다. 후대가 생성된 다른 6개 집단은 모두 집단 내 암수 교미에서 역교잡 F2 세대를 생성하였다. 이 결과는 팥나방 암컷과 어리팥나방 수컷이 교미하였을 경우 F1 후대잡종을 생성할 수 있으나, 이 F1 후대잡종 암컷은 불임이 되는 것을 나타냈다. 결과적으로 두 종 사이에 접합후 생식단계에서 부분적인 생식격리가 발생할 수 있는 것을 나타냈다. Artificial interspecific matings between Matsumuraeses phaseoli and M. falcana (Lepidoptera: Tortricidae) were conducted to know the possibility of hybridization of the two sympatric species. Reciprocal crossings successfully produced F1 hybrids. Most of F2 crosses yielded progenies except all mating trials with females of F1 hybrid obtained from M. phaseoli female. Inbreedings of the F2 hybrids produced F3 progenies. In backcrossings between F1 hybrids and parent lines, all the two mating trials with females of F1 hybrid obtained from M. phaseoli females did not produce any progeny, while other 6 backcrosses produced the next generations. Inbreedings of the backcross lines also produced subsequent progenies. These results indicated that F1 females produced from hybridization between M. phaseoli females and M. falcana males were sterile. Conclusively, it suggested that a partial reproductive isolation at a postzygotic stage can occur between the two sympatric species.

반합성 인공사료에서 팥나방(Matsumuraeses phaseoli)과 콩명나방(Maruca vitrata)의 발육 특성

정진교,서보윤,박종호,문중경,최봉수,이영호,Jung, Jin-Kyo,Seo, Bo-Yoon,Park, Jong-Ho,Moon, Jung-Kyung,Choi, Bong-Su,Lee, Young-Ho 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.3

Two lepidopteran species, Matsumuraeses phaseoli (Matsumura) and Maruca vitrata (syn. M. testulalis) (Fabricius) were reared on artificial diets, and analyzed in their developmental characteristics. Photoperiod was supplied with 16L/8D for M. phaseoli and with 13L/11D for M. vitrata, respectively. Both species passed five larval instars with discrete sizes of head capsule width. In a constant environment ($25^{\circ}C$ and 65%RH), the developmental period of M. phaseoli egg, larva and pupa was 3.9, ca. 16.0 and 8.9 days, respectively, and over 80% of M. phaseoli larvae could develop into pupae, most of which emerged into adults. Newly laid eggs could be stored at $5^{\circ}C$ for 15 days with over 50% hatchability. Similar developmental traits were shown in M. vitrata. However, a low temperature preservation was not applicable to M. vitrata eggs. 팥의 주요해충종인 팥나방(Matsumuraeses phaseoli (Matsumura))과 콩명나방(Maruca vitrata (Fabricius)(syn. M. testulalis)에 대한 인공사료를 이용한 실내 사육 조건(온도 $25^{\circ}C$, 상대습도 60%)에서 기초적인 발육특성이 조사되었다. 팥나방은 16L/8D, 콩명나방에는 13L/11D의 광조건이 제공되었다. 두 곤충종 모두 유충 영기는 두폭이 뚜렷한 불연속 생장을 보이면서 5령을 경과하였다. 팥나방은 알기간, 유충기간, 용기간이 각각 3.9일, 약 16일, 8.9일이었고 유충의 용화율은 80% 이상이었고, 대부분의 번데기가 우화하였다. 갓산란된 알을 섭씨 5도에서 15일 보관할 때 50% 이상이 부화하였다. 콩명나방의 발육도 팥나방과 비슷한 성질을 보였다. 그러나 콩명나방 갓산란된 알이 저온보관된 경우에는 전혀 부화하지 못하였다.

유전체육종을 넘어서 디지털육종 시대를 촉진하기 위한 계획

박수철,조우석,권수진,이우문,김도선,윤호백,최태정,최만수,문중경(Jung-Kyung Moon) 한국육종학회 2021 한국육종학회 공동학술발표집 Vol.2021 No.-

증기화광산란 검출기를 이용한 콩 함유 수용성 탄수화물의 분석

김경하(Gyeong-Ha Kim),황영선(Young-Sun Hwang),안경근(Kyung-Geun Ahn),김기쁨(Gi-Ppeum Kim),김민지(Min-Ji Kim),홍승범(Seung-Beom Hong),문중경(Jung-Kyeong Moon),정명근(Myoung-Gun Choung) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.7

In the present study, a new analytical method was devised for the simultaneous determination of soluble carbohydrates in soybean seeds using high performance liquid chromatography/evaporative light scattering detection (HPLC/ELSD). The limit of quantification (LOQ) for soybean soluble carbohydrates ranged from 5.6~7.6 mg/kg using the HPLC/ELSD method and from 16.2~33.9 mg/kg using the high performance liquid chromatography/refractive index detection (HPLC/RID) method. Therefore, the HPLC/ELSD method was more sensitive than HPLC/RID. The precision values for retention time and peak area of the HPLC/ELSD method were evaluated by inter-day (n=5) and intra-day (n=10) assays using a standard solution. All precision values (CV<2.5%) for soybean soluble carbohydrates were acceptable and fulfilled international acceptance criteria. All linear calibration curves were obtained with a correlation coefficient of R<SUP>2</SUP>>0.999. The contents of soluble carbohydrates for the "Shingikong" (yellow soybean) and "Cheongjakong 3" (black soybean) samples were analyzed using the HPLC/RID and HPLC/ELSD methods. The difference in carbohydrate contents between the two detection methods was significant. Carbohydrate contents in the HPLC/ELSD method were higher than those in the HPLC/RID method. Overall, the HPLC/ELSD method showed satisfactory resolution with a favorable LOQ and reproducibility. Therefore, these results indicate that the HPLC/ELSD method may be applied to determine the contents of soluble carbohydrates in soybean seeds and related food stuffs.

한국 콩 29품종의 물 소모량에 대한 수분이용효율 산정

박세준(Sei Joon Park),박종용(Jong Yong Park),엄기철(Ki-Cheol Eom),문중경(Jung-Kyung Moon) 한국육종학회 2014 한국육종학회지 Vol.46 No.4

Water use efficiency (WUE) is considered as an important characteristics of drought tolerance in crop plant. This study was carried out for determination of WUE of 29 Korean soybean cultivars including PI416937, a representative drought tolerant cultivar in USA, under two different soil water contents, the sustaining 50% of maximum field capacity on control and 25% on drought treatment for 14 days at the late of vegetative growth stage. The WUE of whole plant (g/L) was determined using the measurement of the amount of water use (mL/day/plant), dry weight (g/plant), and relative growth rate (RGR) at 14 days after treatment (DAT). The mean amount of water use of 30 soybeans was 183 and 64 mL/plant/day at control and treatment, respectively, which was decreased 63% of water use under 25% of maximum field capacity. The mean dry weight of 30 soybeans of treatment was decreased 37% compared with that of control. The amount of water use of 30 soybeans was highly correlated with dry weight at 14 DAT, while it was weak correlated with RGR of treatment and not correlated with WUE. The WUE was highly correlated with RGR at control and treatment. The mean WUE of 30 soybean was 2.1 and 2.9g/L at control and treatment, respectively. It means that WUE is increased under drought condition and is the variable characteristics depend on soil water content. The WUE of PI416937 was 3.5g/L at treatment. This study suggested WUEs of 29 Korean soybean cultivars and that the higher WUE Korean cultivars than PI416937 were 3 cultivars, Daepoong (3.8g/L), Danbeak(4.0g/L), and Keumkang (3.8g/L).

저장성 고수율 나물용 신품종 소원콩

박금용(Keum Yong Park),윤홍태(Hong Tae Yun),문중경(Jung Kyung Moon),구자환(Ja Hwan Ku),황종진(Jong Jin Hwang),이석하(Suk Ha Lee),성열규(Yeal Kyu Seung),류용환(Yong Hwan Ryu),정우경(Woo Kyung Chung),이영호(Young Ho Lee),김석동(Seok Do 한국육종학회 2000 한국육종학회지 Vol.32 No.3

헤어리베치 수확 시기에 따른 종자 수량, 발아율 및 경실종자 비율 변화 양상

구자환(Ja Hwan Ku),김민태(Min Tae Kim),손범영(Beom Young Son),이진석(Jin Seok Lee),김정태(Jung Tae Kim),황종진(Jong Jin Hwang),백성범(Seong Bum Baek),문중경(Jung Kyung Moon),권영업(Young Up Kwon) 한국초지조사료학회 2012 한국초지조사료학회지 Vol.32 No.2

This experiment was conducted to investigate an optimum harvest time for hairy vetch (HV) seed production. The seeds were harvested at 5 times, 35, 42, 48, 54, and 58 days after flowering (DAF) in 2009 and 2010. In 2011, 3 times of harvest, 39, 49, and 53 DAF were done at Suwon, middle part of Korea. HV plants were harvested and threshed by manually at each time. Seed yield, yield components, germination rate, hard seed rate, and viviparous germination rate were investigated. The highest seed yield represented 54 DAF in 2009, 42 DAF in 2010, and 49 DAF in 2011. When the triticale, HV support plant, comes to early lodging, the time of highest yield was delayed. 1,000-seed weight linearly increased from 35 DAF to 54 DAF. The later harvested seed showed a trend of increasing germination and hard seed ratio. However, viviparous germination had occurred because of in rainy season. In conclusion, it was presumed that an optimum harvest time for safe seed production in HV should be during 42 DAF ~ 54 DAF before the rainy season considering seed yield, 1,000-seed weight and germination. 본 시험은 친환경농업 및 조사료 용도로 재배가 증가하고 있는 헤어리베치를 대상으로 국내 헤어리베치 종자 채종 시 개화 후 경과 일시에 따른 채종 수량 추이를 조사하여 안전 다수확 채종 기간을 구명하기 위하여 경기도 수원시에 위치한 농촌진흥청 국립식량과학원 시험포장에서 헤어리베치 품종 '청풍보라'를 이용하여 2009년부터 2011년까지 3개년에 걸쳐 시험하였다. 2009년도는 개화 후 35일 (6월 11일), 42일 (6월 18일), 48일 (6월 24일), 54일 (6월 30일), 58일 (7월 4일) 등 5회, 2010년도는 개화 후 35일 (6월 21일), 42일 (6월 28일), 48일 (7월 4일), 54일 (7월 10일), 58일 (7월 14일) 등 5회, 그리고 2011년에는 개화 후 39일 (6월 21일), 49일 (7월 1일), 53일 (7월 5일) 등 3회 수확하여 종자 수량과 발아율을 조사한 결과 종자 수량은 개화 후 경과 일수와 비례하여 증가하다가 개화 후 42일 (2010년도), 49일 (2011년도), 54일 (2009년도)에 정점을 나타낸 이후 도복으로 인한 꼬투리 소실로 수량이 감소하였다. 수확 시기가 늦어질수록 1,000립 중과 발아율이 증가하였으나 장마기 이후에 수확 된 종자는 수발아 종자 발생이 증가하였다. 이상의 본 시험 결과에 의하면 헤어리베치 종자 안전 다수확을 위한 수확 시기는 개화 후 42일에서 54일 사이에 장마 전 기간으로 판단되었다.