생리활성 펩타이드의 피부미용학적 특성 및 활용

모상현(Sang Hyun Moh),정대현(Dai Hyun Jung),김형식(Hyoung Shik Kim),조문진(Moon Jin Cho),서효현(Hyo Hyun Seo),김성준(Sung Jun Kim) 한국생물공학회 2011 KSBB Journal Vol.26 No.6

Bioactive peptides (BAP) showed excellent cosmetic activity than bio-materials such as caffeic acid (CA), gallic acid (GA), and nicotinic acid (NA). Caffeoyl tripeptide-1 (CT-1) is a BAP that is stabilized with Gly-His-Lys (GHK) tripeptide and CA by using Fmoc solid phase peptide synthesis. Digalloyl tetrapeptide-19 (DT-19) is stabilized by combining Lys-Glu-Cys-Gly with GA and nicotinoyl tripeptide-1 (NT-1) is synthesized by GHK and NA. According to experiments, CT-1 has an excellent anti-oxidant function even with a very small amount of 10 ppm CT-1. DT-19’s tyrosinase inhibition activity has the better effect of about 28.57% in 0.01% and 33.33% in 0.005% of concentration and about 7.89% in 0.001% concentration than vitamin-C. In addition, NT-1 is safer than the NA. Almost BAPs like pal-KTTKS, acetyl hexapeptide, and copper tripeptide-1 have the anti-wrinkle effect while DT-19 and NT-1 are applicable for potential BAPs focused on the whitening effect. The three kinds of BAPs like CT-1, DT-19, and NT-1 consisting of amino acids are safe to the skin, and have more excellent stability than bio-materials which are found to be unstable and cause skin irritation. Due to the high biological activity of BAP in the field of skin care, its utilization will increase constantly.

딱지꽃 부위별 추출물의 피부세포에서의 효능 연구

모상현(Sang-Hyun Moh),이정훈(Jeong-Hun Lee),김형식(Hyoung-Shik Kim),여진희(Jin-Hui Yeo) 한국화장품미용학회 2015 한국화장품미용학회지 Vol.5 No.3

As natural plant-based industry has been expanded, the use of oriental medicinal plants as cosmeceutical material was received a lot of attentions in the cosmetic industry. Among various medicinal plants, Potentilla chinensis have been interested in its biological effects. Although several attempts were tried to study its biological effect as medicinal plant, only limited studies tried to evaluate its biological effect as cosmeceutical material. In this study, we examined the possibility of Potentilla chinensis extract as a cosmeceutical material. After extraction of Potentilla chinensis, biological evaluation including anti-inflammation, anti-wrinkle, whitening effect and moisturizing effect was performed as cosmeceutical material. As a result, the Potentilla chinensis extract showed remarkable biological activities through stimulating elastin and aquaporin-3, and inhibiting melanin synthesis, cyclooxygenase-2 expression and expression of metallopeptidase-1, respectively. These results show the potential use of Potentilla chinensis as a functional cosmetic material for anti-inflammatory, wrinkle improvement, skin hydration and whitening effects.

미세조류 추출물 및 MAAs 함유 자외선차단제의 자외선차단지수(SPF) 및 자외선 A 차단지수(PFA) 평가

모상현(Moh, Sang-Hyun),서승석(Suh, Sung-Suk),조문진(Cho, Moon Jin),송미영(Song, Mi Young),황진익(Hwang, Jin-Ik),박미례(Park, Mirye),이택견(Lee, Taek-Kyun) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.5

천연물 유래 자외선차단제 개발을 위해 미세조류 추출물 및 mycosporine-likeaminoacids(MAAs)의 활용가능성을 연구하였다.Chlamycomonashedleyi추출물과 추출물 분획물인 MAAs가 각각 7% 포함된 자외선차단크림을 제조하고 인체 적용시험을 실시하였다.이를 통하여 자외선차단지수(SPF)및 자외선 A차단지수(PFA)를 평가하였다.Ch.hedleyi추출물과 MAAs함유 자외선 차단 크림의 자외선 차단지수는 각각 9.07및 9.42로 나타났으며,자외선 A차단지수는 각각 2.43과 2.41 로 측정되었다.자외선 A차단지수가 모두 2 상으로 측정되어 두가지 자외선 차단크림의 자외선 A차단등급은 [PA+]으로 분류되었다.이상의 연구결과는 미세조류 추출물 및 MAAs함유 자외선차단제가 자외선을 효과적으로 막지 못하지만,무기 자외선 차단제가 혼합되면 만족할만한 자외선 차단효과를 보일 수 있을 것으로 판단된다. For the sunscreen development from natural resources, a possible usage of microalgal extracts or mycosporine-like amino acids (MAAs) was investigated. Sunscreens containing 7% of microalgal extracts or MAAs derived from microalgae, Chlamydomonas hedleyi, were prepared and they were applied to human research. Through this clinical research, the values of Sun Protection Factors, Sun Protector Factor (SPF) and Protection Factor of UVA (PFA), of sunscreen containing microalgal extracts or MAAs were determined: SPF values of microalgal extracts and MAAs indicated 9.07 and 9.42, respectively, while PFA ones did 2.43 and 2.41. Due to more than 2 of PFA value in both sunscreens, they can be classified into [PA+]. Taken together, although sunscreen containing microalgae-derived extracts or MAAs does not effectively protect UV irradiation, its capacity can be satisfied if inorganic UV-protecting compounds are added.

직류전류 인가를 통한 김으로부터 포피라334의 대량 정제 고도화

장성주(Sung Joo Jang),이정훈(Jeong Hun Lee),서효현(Hyo Hyun Seo),이삭(Sak Lee),김지연(Jiyeon Kim),김도연(Doyoun Kim),모상현(Sang Hyun Moh) 한국해양바이오학회 2020 한국해양바이오학회지 Vol.12 No.1

Marine algae contain a variety of substances, such as mycosporine-like amino acids, which can defend against UV irradiation. Among them, Porphyra-334 derived from Porphyra yezoensis is attracting attention as a novel active ingredient for anti-aging cosmetics because of its excellent anti-oxidative, anti-inflammatory, and wound-healing properties through promoting skin cell migration. In this study, a process using direct current (DC) for increasing the yield of large-scale purification of Porphyra-334 was developed. When DC was applied to obtain Porphyra-334 efficiently, the purification time was shortened by approximately 1/4 compared with the process wherein DC was not applied; moreover, the yield of purification was improved.

고삼뿌리 추출물 내 Kurarinone, Oxymatrine, Matrine 분석 방법 검증

장성주(Sung-Joo Jang),이삭(Sak Lee),이정훈(Jeong-Hun Lee),모상현(Sang-Hyun Moh) 한국화장품미용학회 2020 한국화장품미용학회지 Vol.10 No.1

The root of the Sophora flavescens with excellent pharmacological efficacy has been used as a herbal medicine. The test was carried out to verify the analysis of kurarinone, oxymatrine and matrine in the root extract of the Sophora flavescens using HPLC. As a result of confirming the MS/MS by fractionation, it was confirmed that the corresponding peaks were kurarinone, oxymatrine, and matrine, respectively, as the MS/MS spectrum was consistent. As a result of confirming the accuracy test and reproducibility, and the precision within and between batches, it was possible to confirm high accuracy and precision. As a result of confirming the stability of the pre-treated sample, all three materials were stable for 48 hours. In conclusion, it was evaluated and established that the method validation for kurarinone, oxymatrine and matrine in the range with the specificity, linearity, accuracy, precision, and stability.

김유래 미코스포린 유도체인 포피라334가 함유된 화장품 제형의 주름개선 효능 검증

장성주(Sung-Joo Jang),이삭(Sak Lee),백승혜(Seung-Hye Paek),신동선(Dong-Sun Shin),송지혁(Ji Hyeok Song),김재영(Jae-Young Kim),이상준(Sang-Jun Lee),모상현(Sang-Hyun Moh) 한국화장품미용학회 2021 한국화장품미용학회지 Vol.11 No.2

In this study, we focus on the Porphyra 334, one of mycosporine-like amino acids (MAAs). Porphyra 334 was purified from Porphyra yezoensis and its validation method was established. Anti-wrinkle activity of Porphyra 334 on the procollagen synthesis in Human dermal fibroblast (HDF) was evaluated that Porphyra 334 significantly increased synthesis of procollagen in HDF. The clinical test in vivo demonstrated that constant application of Porphyra 334 improved periorbital wrinkles compared with the placebo. In conclusion, Porphyra 334 might be a novel biomaterial for anti-wrinkle according to in vitro in HDF and clinical test in vivo.

Mycosporine-like amino acids (MAAs) 처리에 따른 배양세포 내 스크래피 프리온 단백질의 형성증가

이지현,모상현,류종석,김대환,Lee, Jihyun,Moh, Sang-Hyun,Ryou, Chongsuk,Kim, Dae-Hwan 한국미생물·생명공학회 2015 한국미생물·생명공학회지 Vol.43 No.2

Prion은 양의 scrapie, 소의 bovine spongiform encephalopathy와 사람의 CJD와 같은 다양한 신경 퇴행성 질환을 유발시키는 단백질 병원체이다. 정상 prion 단백질인 PrP^C가 병원성 PrP^Sc로 바뀌는 과정에 대해서는 많은 연구가 진행되었고, PrP^Sc로의 단백질 구조 변화가 다양한 환경적 요소에 의해서 영향 받는 것으로 추측된다. 바다조류로부터 분리된 MAAs는 다양한 스트레스 환경에서 조류를 보호해주는 것으로 알려져 있다. 이와 같은 사실에 기초하여 mycosporineglycine, porphyra-334와 shinorine 3종의 MAAs로 처리한 prion 감염 신경세포 주에서 prion 단백질 축적의 변화를 평가하였다. PK 저항성을 갖는 PrP^Sc를 western blot 방법으로 확인한 결과, MAA에 의해서 PrP^Sc 단백질의 증식을 관찰하였다. Prions are proteinaceous infectious particles that cause neurodegenerative diseases, such as scrapie in sheep, bovine spongiform encephalopathy in cattle and Creutzfeldt-Jakob disease (CJD) in humans. Although the detailed process, regarding the abnormal conversion of prion proteins (PrP), remains to be fully elucidated, a number of environmental factors appear to affect the formation of misfolded PrP, termed PrP^Sc. Because oceanic algae contain mycosporine-like amino acids (MAAs), which exhibit cellular defensive activities under a variety of stress conditions, we investigated the level of PrP^Sc in prion-infected neuroblastoma cells using mycosporine-glycine, porphyra-334 and shinorine. When judged by the level of protease-resistant PrP^Sc in western blots, porphyra-334 and shinorine increased the level of PrP^Sc in cells, but mycosporine-glycine did not. The current results indicate that the MAAs tested in this study enhance the formation of PrP^Sc.

식물세포배양기술을 이용한 순무 캘러스 추출물의 생리활성

신수영,모상현,황유진,Shin, Su Young,Moh, Sang Hyun,Hwang, You Jin 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.2

본 연구에서는 인천광역시 강화군의 특화작물을 이용한 기능성 소재를 개발하기 위해 순무 캘러스를 배양하고 열수, 초음파 그리고 에탄올 추출하여 생리활성을 평가하였다. 순무 캘러스의 열수, 초음파 그리고 에탄올 추출물에서 DPPH radical소거활성이 대조군에 비해 농도 의존적으로 증가하였다. 순무 캘러스 에탄올 추출물의 수렴효과는 열수 및 초음파 추출물보다 높게 나타났다. 콜라겐 생합성량을 측정한 결과 순무 캘러스 에탄올 추출물의 5 ppm과 10 ppm 농도에서 59%와 78%의 주름개선 효과를 확인하였다. 이러한 결과들은 순무 캘러스의 열수, 초음파 그리고 에탄올 추출물이 천연 항산화 소재로의 활용이 가능할 것으로 사료되며 특히, 에탄올 추출물은 수렴효과와 주름개선 효과가 뛰어나 기능성 화장품 천연소재로서의 활용이 가능할 것으로 기대된다. The purpose of this study was to investigate biological activities of Brassica rapa (Turnip) plant callus extracts of Ganghwa-gun of Incheon city using water, ultrasonic wave and ethanol extractions to develop functional materials. DPPH radical scavenging activities of the callus extracts were increased in a concentration-dependent manner, as compared with control. The astringent effects of the ethanol extracts were higher, as compared to water and ultrasonic extracts. In the collagen synthesis assay, the ethanol extract showed significant anti-wrinkle effects of 59% and 78% at a concentration of 5 ppm and 10 ppm, respectively. These results suggested that water, ultrasonic wave and ethanol extracts of turnip plant calluses are natural antioxidant sources. Especially, the ethanol extract can be regarded as a functional, natural cosmetic material with astringent and anti-wrinkle effects.

Chlamydomonas sp. 유래 mycosporine-like amino acid 혼합물의 항주름 활성

서승석(Suh, Sung-Suk),서효현(Seo, Hyo Hyun),이정훈(Lee, Jung-Hun),황진익(Hwang, Jin-Ik),박미례(Park, Mirye),모상현(Moh, Sang-Hyun),이택견(Lee, Taek-Kyun) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.8

미세조류 유래 mycosporine-like amino acids (MAAs) 혼합물의 항주름활성을 분석하기 위하여 type I procollagen, elastin 및 involucrin 유전자 발현분석을 실시하였다. Chlamydomonas sp.를 80% 에탄올로 추출하고, 컬럼을 통해 분획물을 정제하고, HPLC 분석을 통해 asterina 330+palythine (A+P) 및 shinorine+palythine (S+P) 혼합물을 정제하였다. MTT assay 결과 A+P 및 S+P는 0.1 mg/mL까지 세포독성을 나타내지 않았다. UV에 노출된 섬유아세포에 대한 MAAs 혼합물의 영향을 분석하였을 때, 0.05 mg/mL A+P 및 0.01 mg/mL S+P 농도에서 각각 2.7 및 3.6 배 PCOLCE mRNAs 발현이 증가하였다. Elastin 유전자는 0.01 mg/mL의 A+P 및 S+P를 처리하였을 때 각각 5.59배 및 3.1배 발현이 증가하였다. 특히 0.01 mg/mL의 A+P 및 S+P 농도에서 involucrin mRNAs 발현이 UV 처리된 대조구와 비교하였을 때 5배 및 2.5배 감소하였다. 결론적으로 Chlamydomonas sp. 유래 MAAs 혼합물은 주름개선용 기능성 화장품 개발을 위한 소재로써의 가능성이 충분한 것으로 판단 되었다. To examine the effects of a mycosporine-like amino acids (MAAs) mixture from microalgae, Chlamydomonas sp, on the anti-wrinkle activities, the expression levels of genes that are associated with skin aging, including type I procollagen, elastin and involucrin, were analyzed. Asterina 330+palythine (A+P) and shinorine+palythine (S+P) mixtures were purified from Chlamydomonas sp using the following steps: 80% methanolic extraction, column purification, and HPLC analysis. As a result of the MTT assay, A+P and S+P did not induce cellular cytotoxicity with up to 0.1 mg/mL of both MAAs. In addition, the treatment of UV-exposed fibroblasts with A+P (0.05 mg/mL) and S+P (0.01 mg/mL) increased the levels of the PCOLCE mRNAs by 2.7 and 3.6 fold compared to the control group, respectively, The levels of elastin gene expression were elevated 5.59 and 3.1 fold in the A+P and S+P treated (0.01 mg/mL) cells, respectively. In particular, at a concentration of 0.01 mg /mL, the A+P and S+P expression levels of Involucrin mRNAs were increased 3.5 and 2.5 fold in the UV-exposed cells compared to the control, respectively. In conclusion, the MAAs derived from Chlamydomonas sp can be utilized as functional cosmetic materials with anti-wrinkle effects on the skin.

Chlamydomonas hedleyi 미세조류 형질전환체의 고주파처리 배양방법을 통한 MAA생산량 증가

서효현(Hyo Hyun Seo),송미영(Mi Young Song),아툴 쿨카르니(Atul Kulkarni),서승석(Sung-Suk Suh),이택견(Taek-Kyun Lee),모상현(Sang Hyun Moh) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-

본 연구에서는 빛이 없는 조건에서도 미세조류가 잘 성장할 수 있도록 형질전환 미세조류체를 이용하 여 생물반응기 내에서 배양하며 고주파 처리를 하여 MAAs의 합성 증대량을 조사하였다. 특히, Chlamydomonas hedleyi에 Glucose transporter 유전자인 Glut-1을 도입하여 형질전환체를 만들고, 이 형질전환체를 고주파 처리 조작이 가능하도록 장치된 생물반응기내에서 배양하며 MAAs 생산량을 증가시켰다. 이는 생물반응기 내에서 고주파 처리를 통한 자외선 A 흡수물질을 인위적으로 증폭시킬 수 있어서, 대량배양 후 이 MAAs물질을 분리정제하여 자외선 차단과 관련 화장품 산업화에 크게 기 여할 것이라 사료된다.