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먹이제한과 단기 수온하강 조건에서 넙치의 스트레스 반응과 생리학적 대사활성 변화
명정인 ( Jeong In Myeong ),강덕영 ( Duk Young Kang ),김효찬 ( Hyo Chan Kim ),이정호 ( Jeong Ho Lee ),노재구 ( Jae Koo Noh ),김현철 ( Hyun Chul Kim ) 한국어류학회 2011 韓國魚類學會誌 Vol.23 No.2
To find the preliminary environmental conditions for a short-time transport of living olive flounder, Paralichthys olivaceus, the stress response and physiological metabolic activity of the cultured fish to feed deprivation and slow temperature descending (15.8℃→13.3℃) were monitored for 8 days. The monitored variables were the plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose (GLU), total protein (TP), electrolytes (Na+, K+, Cl-) and thyroid hormones (TT4, TT3, FT4 and FT3). In food deprivation experiment for 8 days, we did not find any statistical change of level in AST, ALT and electrolytes (Na+, K+, Cl-), but found a significant decrease in TP and GLU. In thyroid hormones, the levels of four hormones in plasma were all showing a tendency to decrease. Especially, FT4 and TT3 were significantly decreased, indicating a withering of physiologic activity. In the temperature test, although no any significant change in AST, TP and electrolytes (Na+, K+, Cl-), we observed a significant decrease of ALT and GLU following to temperature descending from 15.8℃ to 13.3℃ (P<0.05). In the levels of thyroid hormones, any significant change was not observed for experimental period. We conclude that the stress response and physiological activity of olive flounder were more influenced by feed deprivation than slow temperature descending at a transport of living fish, and plasma GLU appears to be sensitive factor to physiological metabolic activity, indicating that it could be used as a monitering mark or index for a health inspection of the fish.
감성돔(Acanthopagrus schlegelii) 치어의 포르말린에 대한 내성 및 조직학적 반응
명정인 ( Jeong In Myeong ),민병화 ( Byunng Hwa Min ),박미선 ( Mi Seon Park ),황형규 ( Hyung Kyu Hwang ),도정완 ( Jeung Wan Do ),정경일 ( Kyung Il Jeoung ),장영진 ( Young Jin Chang ),정달상 ( Dal Sang Jeong ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.6
Black seabream juveniles Acanthopagrus schlegelii held at 20°C were exposed to formalin at concentrations of 50 to 400 ppm, and tolerance values were determined by calculating median lethal concentration values (LC50) through probit analysis. The 12-, 24,48, 72- and 96-h LC50 values for formalin were 297, 233, 171, 162 and 157 ppm, respectively. The histological effects of formalin on gill and liver tissues in this fish were determined. No histological effects were observed in the control group. The intensity of cell damage increased with the concentration of, and duration of exposure to, formalin. Hyperplasia, separation and epithelial necrosis,epithelial lifting, lamellar synechiae and collapsed lamellae were observed in gill tissues exposed to formalin. Hepatic lesions in liver tissues of fishes exposed to formalin were characterized by cloudy swelling of hepatocytes, necrosis, cytoplasmic vacuolization,deposition of pigments, spongiosis hepatis, nuclear hypertrophy, dilation of sinusoids and bile stagnation. The LC50 values and histological results obtained in this study will aid in designing treatment regimens to minimize toxic side effects and increase efficacy.
참돔(Pagrus major)에서 온도 및 염분 스트레스가 FK506BP 발현에 미치는 영향
민병화,명정인,강한승,Min, Byung Hwa,Myeong, Jeong-In,Kang, Han Seung 한국해양생명과학회 2017 한국해양생명과학회지 Vol.2 No.1
FK506BP (FK506 binding protein 12) is a small peptide with a single FK506BP domain. It is involved in suppression of immune response, oxidative stress and inflammation. The purpose of this study was to investigate the gene expression of FK506BP in red seabream (Pagrus major) exposure to low water temperature (8℃, 33 psu) and low salinity (20℃, 10 psu). Results showed that, the expression of FK506BP was significantly increased in the experiment groups, such as low water temperature (8℃, 33 psu), and low salinity (20℃, 10 psu). These results suggest that FK506BP was played roles in biomarker gene on the environmental stress such as water temperature and salinity.
급격한 염분변화에 따른 수온별 참전복( Haliotis discus hannai) 치패의생존율 및 체성분 변화
정민환 ( Min Hwan Jeong ),김성희 ( Seong Hee Kim ),박미선 ( Mi Seon Park ),김강웅 ( Kang Woong Kim ),장영진 ( Young Jin Chang ),명정인 ( Jeong In Myeong ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.5
Physiological studies on the salinity tolerance with respect to survival rate and body composition changes of the abalone Haliotis discus hannai were conducted by rapidly changing the salinity in an indoor rearing system. The survival rate of the control (35 psu), 30 and 25 psu groups at 15℃ was 100%. The survival rate of the 20 psu group was 35±5.0%. Survival rate of the 15 psu group was 0%. At 15℃ and 35, 30, 25, 20, 15 psu, the moisture contents of abalone muscle were 82.1±0.7, 82.5±0.7, 84.9±0.5, 86.9±0.3 and 86.6±0.4%, respectively. Crude lipid contents were 0.47±0.03, 0.47±0.03, 0.47±0.09, 0.77±0.09 and 0.63±0.03%, respectively and crude ash contents were 1.30±0.12,1.33±0.15, 1.13±0.23, 1.87±0.15 and 1.40±0.31%, respectively. At salinity below 20 psu, these values increased compared with the control. The general components of abalone muscles significantly increased below 20 psu, while amino acid composition showed no significant difference with salinity and water temperature.