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록용약침액(鹿茸藥鍼液)의 위암세포주(胃癌細胞柱)에 대한 Oligonucleotide Chip 분석
류성현 ( Sung Hyun Ryu ),이경민 ( Kyung Min Lee ),이봉효 ( Bong Hyo Lee ),임성철 ( Seong Chun Lim ),정태영 ( Tae Young Jun ),서정철 ( Jeung Chul Seo ) 대한경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.2
Objectives: It has Long been known about the osteogenic effect of CPC-HAS(cervi parvum cornu herbal-acupuncture solution) on bone tissues. However, it has not been determined the effect of CPC-HAS on cancer cells. The purpose of this study is to screen the CPC-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell Lines. Oligonucleotide microarray approache was employed to screen the differential expression genes. Methods: CPC-HAS was prepared by boiling and stored at -70℃ until use. Cells were treated with various concentrations of CPC-HAS (0.1, 0.5, 1.5, 10, 20 ㎎/㎖) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5 ㎎/㎖ of CPC-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip(Human genome U133 Plus 2.0., Affimatrix Co.). Results: It has no cytotoxic effects on SNU484 cell in all concentrations(0.1, 0.5, 1.5, 10, 20 ㎎/㎖). In oligonucleotide microarray assay, in SNU484 cells, the number of more than twofold up-regulated genes was 5 while, the number of more than twofold down-regulated genes was 10. Conclusions: This study showed the screening of CPC-HAS mediated differentially regulated genes using combined approaches of oligonucleotide microarray. The screened genes will be used for the better understanding of the therapeutic effects of CPC-HAS on cancer fields.
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실리카 템플레이트를 이용하여 다공성 중공형태를 갖는 LiMn<sub>2</sub>O<sub>4</sub> 합성 및 전기화학적 특성 연구
류성현,류광선,Ryu, Seong-Hyeon,Ryu, Kwang-Sun 한국전기화학회 2011 한국전기화학회지 Vol.14 No.3
다공성 중공형태의 $LiMn_2O_4$는 실리카 템플레이트과 침전법에 의해 합성되었다. 합성한 $LiMn_2O_4$는 나노사이즈의 1차입자를 가지며 다공성 중공형태를 가지고 있었다. 실리카 템플레이트의 제거는 NaOH를 이용하여 화학적 에칭법이 사용되었다. NaOH의 농도를 높여줌에 따라 망간산화물 입자 크기가 증가 하며 다공성의 중공구가 형성되었다. X-선 회절 분석을 통하여 합성된 $LiMn_2O_4$는 Fd3m의 공간 그룹을 가지는 스피넬 구조가 형성된 것을 확인 할 수 있었다. 실리카와 망간염의 비율을 높여주었을 경우 합성된 $LiMn_2O_4$는 1차입자의 크기는 감소한다. 실리카와 망간염의 비율이 1 : 9 이상인 경우에서 마이크론 단위의 정방정계의 $LiMn_2O_4$가 합성되었다. 다공성 중공형태의 $LiMn_2O_4$의 전기화학적 특성을 평가하기 위하여 2032형태의 코인셀을 제작하여 충/방전 테스트를 하였다. 나노사이즈의 1차입자를 가진 시료의 경우에는 마이크론 사이즈의 1차입자를 가진 시료보다 용량은 낮았지만 용량유지율은 향상되는 것 확인 할 수 있었다. $LiMn_2O_4$ with mesoporous hollow sphere shape was synthesized by precipitation method with silica template. The synthesized $LiMn_2O_4$ has nanosized first particle and mesoporous hollow sphere shape. Silica template was removed by chemical etching method using NaOH solution. When the concentration of NaOH solution was increased, first particle size of manganese oxide was decrease and confirmed mesoporous hollow shpere shape. X-ray diffraction(XRD) patterns revealed that the synthesized samples has spinel structure with Fd3m space group. In case the ratio of silica and maganese salt increased, the size of first particles was decreased. The tetragoanal $LiMn_2O_4$ with micron size was synthesized at ratio of silica and manganese salt over 1 : 9. The prepared samples were assembled as cathode materials of Li-ion battery with 2032 type coin cell and their electrochemical properties are examined by charge-discharge and cyclic performance. Electrochemical measurements show that the nano-size particles had lower capacity than micron-size particles. But, cyclic performance of nano-size particles had better than that of micron-size particles.
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시간사용중재가 뇌졸중 환자의 생활만족도 및 자아존중감에 미치는 영향
류성현(Ryu, Sung-Hyun),박수진(Park, Su-Jin),김민혁(Kim, Min-Huck),황도연(Hwang, Do-Yeon) 대한신경계작업치료학회 2020 재활치료과학 Vol.9 No.3
목적 : 본 연구는 뇌졸중 환자를 대상으로 8주간 실시된 시간사용중재가 뇌졸중 환자의 생활만족도 및 자아존중감에 미치는 영향을 알아보았다. 연구방법 : 본 연구는 2019년 7월부터 10월까지 뇌졸중 환자 21명을 대상으로 실험군과 대조군으로 나누어 실시하였다. 생활만족도의 변화는 생활만족도 척도(Life satisfaction scale)를 사용하였고, 자아존중감의 변화는 자아존중감 척도(Self-esteem scale)를 사용하였다. 결과 : 첫째, 실험군과 대조군 각각 중재 전과 후를 비교한 결과, 생활만족도와 자아존중감 모두 통계학적으로 유의한 차이를 보였다(p<.05). 둘째, 중재 후 실험군과 대조군의 생활만족도와 자아존중감을 비교한 결과, 통계학적으로 두 집단 간 유의한 차이를 보였다(p<.05). 결론 : 본 연구의 결과 시간사용중재가 뇌졸중환자의 생활만족도와 자아존중감에 긍정적인 영향을 미쳤으며, 따라서 의미있는 작업 수행의 참여가 뇌졸중 환자의 정신건강과 생활의 질을 향상시킬 수 있을 것이다. Objective : This study investigated the effects of time-use intervention, conducted during 8 weeks, on the life satisfaction and self-esteem of stroke patients. Methods : Twenty-one stroke patients who were divided into an experimental group (n=10) and a control group (n=11), participated in this study from july 2019 to october 2019. Life satisfaction changes were measured using the Life satisfaction scale. Self-esteem changes were measured using the Self-esteem scale. Results : First, comparison between pre and post intervention showed a statistically significant difference in life satisfaction and self-esteem (p<.05). Second, comparison between post intervention groups showed a statistically significant difference in life satisfaction and self-esteem (p<.05). Conclusion : Based on the results from this study, we found that time-use intervention has a positive impact on life satisfaction and self-esteem. Therefore, participating in meaningful occupation performance will improve the mental health and quality of life of stroke patients.
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녹용약침액(鹿茸藥鍼液)의 간암세포주(肝癌細胞柱)에 대한 DNA 및 단백질 발현(發顯)
류성현,이경민,이봉효,임성철,정태영,서정철,Ryu, Sung-Hyun,Lee, Kyung-Min,Lee, Bong-Hyo,Lim, Seong-Chul,Jung, Tae-Young,Seo, Jung-Chul 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.2
Objective : It has long been known about the osteogenic effect of CPC-HAS on bone tissues. However, it has not been determined the effect of CPC-HAS on cancer cells. The purpose of this study is to screen the CPC-HAS mediated differentially expressed genes in cancer cells such as HepG2 hepatoma cells. Oligonucleotide microarray and proteomics approaches were employed to screen the differential expression genes. Methods : CPC-HAS was prepared by boiling and stored at $-70^{\circ}C$ until use. Cells were treated with various concentrations of CPC-HAS (0.1, 0.5, 1.5, 10, 20mg/ml) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5mg/ml of CPC-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip(Human genome Ul33 Plus 2.0., Affimatrix Co.). For proteomic analysis, total protein was analyzed by 2D gel electrophoresis and Q-TOF mass spectrometer. Results : It has no cytotoxic effects on both HepG2 cell in all concentrations(0.l, 0.5, 1.5, 10, 20mg/ml). In oligonucleotide microarray assay, the number of more than twofold differentially regulated known genes was 23 with 5 up-regulated and 18 down-regulated genes in HepG2 cells. In proteomic analysis, three spots were identified by 2D-gel electrophoresis and Q-TOF analysis. Two down-regulated proteins were aldehyde dehydrogenase 1 and enolase 1, and up-regulated protein was fatty acid binding protein 1 by 1.5mg/ml of CPC-HAS. Discussion : This study showed the screening of CPC-HAS mediated differentially regulated genes using combined approaches of oligonucleotide microarray and proteomic analysis. The screened genes will be used for the better understanding of the therapeutic effects of CPC-HAS on cancer fields.
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한진희,류성현,Hahn, Chinhee,Ryu, Sunghyun 한국개발연구원 2010 韓國 開發 硏究 Vol.32 No.3
본 논문에서는 1990년대 중반 이후 우리나라 교역조건의 추세적 하락요인을 Baxter and Kouparitsas(2000)의 분해방법론을 이용하여 분석해 보았다. 주요 분석결과는 다음과 같다. 교역조건의 변화의 분해방법을 통해 우리나라 교역조건의 하락추세가 대부분 공산품 대비 연료의 상대가격 상승으로 인한 재화상대가격효과에 기인함이 나타났다. 또한 전 세계 55개국의 교역조건 변화를 동일한 방법론으로 분해해 본 결과, 1990년대 중반 이후 재화상대가격효과에 의한 교역조건의 추세적 하락이 선진국 개도국을 불문하고 공산품 수출국의 공통적인 현상임을 보였다. 이는 우리나라 교역조건 하락의 주요 원인이 우리나라 내부적 요인보다는 예를 들어 중국의 무역확대 등 외부적 요인에 기인함을 시사한다. 회귀분석 결과 중국의 무역확대는 우리나라의 교역조건 하락의 중요한 원인이 되었으며, 특히 2000년대의 원유와 원자재 수입가격의 상승과 공산품의 수출가격을 하락시킨 것으로 나타났는데, 이는 교역조건 분해결과가 시사하는 바와 부합한다. This paper examines the causes of the terms of trade decline in Korea since the mid-1990s, using the decomposition methodology suggested by Baxter and Kouparitsas (2000) as well as regression analysis. The main empirical results are summarized as follows. The decomposition exercise of changes in terms of trade showed that Korea's terms of trade decline for the past decade or so is attributable to goods price effect which were driven by the rise of oil prices relative to manufactures. The decomposition of terms of trade change for 55 countries showed that terms of trade decline due to goods price effect is a phenomenon that was commonly observed for exporters of manufactures since mid-1990s. These results suggest that external factors such as China's trade expansion, rather than internal factors, are mostly responsible for the decline in terms of trade. In accordance with these results, the regression results suggest that China's trade expansion contributed to Korea's terms of trade decline, especially in 2000s, by raising the import prices of oil and raw materials and lowering the export prices of manufacturing products.
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조성우,주정훈,류성현,류광선,Cho, Sung-Woo,Ju, Jeong-Hun,Ryu, Seong-Hyeon,Ryu, Kawng-Sun 한국전기화학회 2010 한국전기화학회지 Vol.13 No.4
$LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$의 리튬이온 이차전지 양극 물질로의 특성을 연구하기 위해서 단순 연소합성법을 이용하여 합성했다. 합성된 물질의 구조적 특징을 분석하기 위하여 X-선 회절분석(XRD)과 주사전자현미경 (FE-SEM)을 측정하였다. X-선 회절분석을 통하여 합성된 $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$시료가 육방정계 층상구조가 형성된 것을 확인하였다. FE-SEM을 통해 측정한 결과 $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$ 입자는 일정한 형태를 가지지 않았으며 크기는 대략 100~300 nm의 크기임을 확인할 수 있었다. 그리고 전기화학적 특성을 측정하기 위하여 충 방전 용량 측정과 CV(Cyclic Voltammetry)를 측정하였다. 2.8 V에서 4.3 V까지 충 방전 용량을 측정한 결과 ~162 mAh/g의 초기 방전 용량을 가졌다. $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$ active material was prepared by simple-combustion method and investigated as the cathode material for li-ion battery. The structural characterization was analyzed by X-ray diffraction (XRD) and field emission scanning electron microscopy (FE-SEM), respectively. The XRD patterns of $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$ sample was indicated a phase of layered hexagonal structure. The size of particles has not uniform diameters ranging from 100 to 300 nm. The electrochemical performance of the $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$ was measured by Cyclic Voltammetry and galvanostatics. The $LiNi_{0.5}Mn_{0.3}Co_{0.2}O_2$ shows the discharge capacity of ~162 mAh/g in the range of 2.8 to 4.3 V at the first cycle.
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