Subsea System 최적 설계 요소에 관한 연구

최한석(Han-Suk Choi),도창호(Chang-Ho Do),이승건(Seung-Keon Lee) 대한조선학회 2012 大韓造船學會 論文集 Vol.49 No.4

해저 파이프라인 열좌굴 해석방법에 관한 연구

양승호,정종진,이우섭,도창호,Yang, Seung-Ho,Jung, Jong-Jin,Lee, Woo-Sub,Do, Chang-Ho 대한조선학회 2010 大韓造船學會 論文集 Vol.47 No.2

The requirement of design of High-Pressure/High-Temperature(HP/HT) pipelines on an seabed increases in recent years. The need of research on the analysis method to improve the design capacity is increasing. The purpose of this study is the development of the analysis method of thermal buckling of subsea pipeline structures. The analysis method of thermal buckling was established by using the commercial FEM code(ABAQUS) which shows the outstanding performance in non-linear static FE analysis. The developed method has been applied to the installation of subsea pipeline on the offshore project. For a validation, the comparative study has been carried out. This application to offshore project demonstrates the superiority of the analysis method of thermal buckling of subsea pipeline structures and testifies the application to detail design.

K562 세포주에서 Genistein에 의해 억제되는 Radiation-induced Apoptosis의 조절 유전자

정수진,진영희,유여진,도창호,정민호,허기영,배혜란,양광모,문창우,오신근,허원주,이형식,Jeong, Soo-Jin,Jin, Young-Hee,Yoo, Yeo-Jin,Do, Chang-Ho,Jeong, Min-Ho,Huh, Gi-Yeong,Bae, Hye-Ran,Yang, Kwang-Mo,Moon, Chang-Woo,Oh, Sin-Geun,Hur, Won-Jo 대한방사선종양학회 2001 Radiation Oncology Journal Vol.19 No.3

목적 : K562 세포를 대상으로 PTK inhibitor (herbimycinA와 genistein)에 의한 방사선 유도 apoptosis의 변화에 따른 관련 유전자를 탐색하고자 하였다. 대상 및 방법 : K562 세포는 $2\times10^5\;cells/mL$의 비율로 준비하여 대수증식기로 성장한 세포를 각각의 실험조건에 따라 처리하여 사용하였다. 방사선 조사는 6-MV X-Ray 10 Gy (Clinac 1800C, Varian, USA)를 $200\~300\;cGy/min$의 선량율로 상온에서 일정하게 조사하였다. Herbimycin A (HMA, Calbiochem, UK)와 genistein (Calbiochem, UK)은 dimethylsulfoxide (DMSO, Sigma, UK)에 녹여서 각각 1 mM과 10 mM의 농축용액으로 조제한 각각 250 nM과 $25\;{\mu}M$의 최종농도로 처리하였다. 내성 변화의 조절에 관여하는 유전자를 찾고자 PCR-select cDNA subtractive hybridization을 실시하여 128개의 차별 발현되는 clones을 재선별하였다. DNA sequencing을 통하여 염기서열을 분석하였으며, GenBank database를 이용하여 이미 밝혀진 유전자들과의 상동성을 비교하였다. 상동성을 갖는 clone을 확인하여 이를 probe로 사용하여 방사선 단독조사한 실험군과 방사선과 HMA 또는 genistein을 동시 처리한 실험군들간의 mRNA 발현의 차이를 Northern hybridization을 통하여 확인하였다. 결과 : homo sapiens Smad6 유전자와 $95\%$의 상동성을 갖는 clone을 확인하였다. 이를 probe로 사용하여 방사선 단독 조사한 실험군과 방사선과 HMA 또는 genistein을 동시 처리한 실험군들간의 mRNA 발현의 차이를 비교 분석한 결과, 방사선과 genistein을 동시 처리한 실험군의 mRNA 발현이 방사선 단독 조사한 실험군과 방사선과 HMA를 처리한 실험군들에 비하여 현저히 높았다. 결론 : Smad6와 방사선에 의해 유도되는 apoptosis의 억제에 관한 연관성을 보고한 문헌은 없으나, Smad6가 일부세포에서 apoptosis를 억제한다는 보고들과, 본 연구에서 관찰한 genistein에 의한 방사선에 의해 유도되는 apoptosis의 억제에서 그 발현이 두드러지게 증가한 점 등을 미루어 보아 방사선에 의하여 유도된 apoptosis의 억제 및 방사선 내성의 조절에도 관여할 것으로 생각된다. Prupose : The genes involved on the suppression or radiation-induced apoptosis by genistein in K562 leukemia cell line was investigated. Materials and methods : K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. For X-ray irradiation and drug treatment, cultures were prepared at $2\times10^5\;cells/mL$. The cells were irradiated with 10 Gy (Clinac 1800C, Varian, USA), Stock solutions of herbimycin A (HMA, Calbiochem, UK) and genistein (Calbiochem, UK) were prepared in dimethylsulfoxide (DMSO, Sigma, UK). After incubation at $37^{\circ}C$ for 24 h, PCR-select cDNA subtractive hybridization, dot hybridization, DNA sequencing and Northern hybridization were examined. Results : Smad6 gene was identified from the differentially expressed genes in K562 cells incubated with genistein which had been selected by PCR-select cDNA subtractive hybridization. The mRNA expression of Smad6 in K562 cells incubated with genistein was also higher than control group by Northern hybridization analysis. Conclusion : We have shown that Smad6 involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line. It is plausible that the relationship between Smad6 and the suppression of radiation-induced apoptosis is essential for treatment development based on molecular targeting designed to modify radiation-induced apoptosis.

간장 및 담도 : 간상해를 동반한 당뇨병 - 특히 HBs Ag 양성빈도 및 동위원소 간 소견 -

유방현(Bang Hyun Liu),김성은(Seong Eun Kim),도창호(Chang Ho Do),이형유(Hyeong Yoo Lee),조몽(Mong Jo) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.1

N/A To evaluate of the possibility of diabetes mellitus in the precipitating factor of liver cirrhosis, the authors have made a clinical analysis on 56 patients of diabetes mellitus with chronic hepatopathy (Cirrhotic Liver Group (29 cases): Noncirrhotic Liver Group (27 cases)} who were admitted at Pusan National University Hospital from Feb. 1987 to Jun. 1989. The results were as follows; 1) The mean age of DM group with cirrhosis was significantly older than that of DM group without cirrhosis (p<0.01). 2) On duration of DM, especially more than 6 years, DM group with cirrhosis was longer than that of DM group without cirrhosis (62% : 32%). 3) On fasting blood sugar level, especially more than 201 mg%, DM group with cirrhosis was higher than that of DM group without cirrhosis, and in cirrhotic group complications of portal hypertension were higher in high FBS level cases. 4) On body weight, especially range of IBW +- 10%, DM group with girrhosis was less controlled than DM group without cirrhosis (41% : 59%). 5) On complications of DM, both group are 76% and 71%, respectively, and the high incidence of complication suggest that duration of DM was long. 6) The positive rate of HBs Ag in DM group with cirrhosis was significantly higher than that of DM group without cirrhosis (28% : 4%, p<0.05). 7) On chemical liver function studies, DM group with cirrhosis showed more increased abnormal value except SGPT, total protein than that of DM group without cirrhosis. 8) On comparison of liver size by radiosotope liver scan, DM group with cirrhosis was smaller than that of DM group without cirrhosis (9.70 +- 1.59:12.68 +- 1.16, p<0.001). 9) On radiosotope liver scan, mottling of DM group with cirrhosis was coarse, but DM group without cirrhosis was fine (p<0.005), and spleen visualization in DM group with cirrhosis was more significant than that of DM group without cirrhosis (92% : 40%, p<0.001).

멕시코만의 BP사 오일유출 해저 대책에 대한 분석

최한석(Han-Suk Choi),이승건(Seung-Keon Lee),도창호(Chang-Ho Do) 한국해양공학회 2011 韓國海洋工學會誌 Vol.25 No.3

On April 20, 2010, a well control event allowed hydrocarbon (oil and gas) to escape from the Macondo well onto Deepwater Horizon (DWH), resulting in an exploration and fire on the rig. While 17 people were injured, 11 others lost their lives. The fire continued for 36 hours until the rig sank. Hydrocarbons continued to flow out from the reservoir through the well bore and blowout preventer (BOP) for 87 days, causing an unprecedented oil spill. Beyond Petroleum (BP) and the US federal government tried various methods to prevent the oil spill and to capture the spilled oil. The corresponding responses were very challenging due to the scale, intensity, and duration of the incident that occurred under extreme conditions in terms at pressure, temperature, and amount of flow. On July 15, α capping stack, which is another BOP on top of the existing BOP, was successfully installed, and the oil spill was stopped. After several tests and subsea responses, the well was permanently sealed by a relief well and a bottom kill on September 19. This paper analyzes the subsea responses and engineering efforts to capture the oil, stop the leaking, and kill the subsea well. During the investigation and analysis at subsea responses, information was collected and data bases were established for future accident prevention and the development of subsea engineering.

간경변증의 중증도에 관한 연구 - HBsAg 양성도와 습관적 음주의 영향에 관한 고찰 -

유방현(Bang Hyun Liu),김성은(Seong Eun Kim),도창호(Chang Ho Do),이형유(Hyeong Yoo Lee),이용규(Yong Kyu Lee) 대한소화기학회 1989 대한소화기학회지 Vol.21 No.3

N/A The severity and other clinical features were assessed in a series of 80 patients with postnecrotic liver cirrhosis, based on Pughs modification of Child- Turcotte classification (CTC), according to the HBsAg positivity and the history of habitual alcohol intake, respectively. Among 80 patients, male was 66%, habitual alcoholics was 48%, of which 95% was male. HBsAg was positive in 45%, HBsAg positive and negative group showed no difference in the severity estimated by CTC, but the habitaual alcoholics showed more severe tendency than the remained(p<0.05). The difference was prominent in variables of serum albumin, bilirubin, and prolongation of prothrombin time (p<0.01). In addition, the number of alcoholics with increased serum gamma-GTP and of serum ALT level was greater than that of non-alcoholics (p<0.01), while HBsAg positive group has no significant difference from HBsAg negative group in chemical liver function test. On the other hand, non-alcoholics had more splenomegaly and hypersplenism (p<0.005), but no more esophageal varix.

원발성 간세포암의 자연병력 및 예후에 관한 연구

허윤(Yoon Hur),문한규(Han Kyu Moon),양웅석(Ung Suk Yang),김성은(Seong Eun Kim),백대근(Dae Geun Baek),도창호(Chang Ho Do),송근암(Guen Am Song),유방현(Band Hyun Liu) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.2

N/A Hepatocellular Carcinoma (HCC) is considered a highly malignant tumor with a poor prognosis. It is usually not resectable and there is no other effective treatment. The current study was undertaken to evaluate the survival time of patients wit.h hepatocellular carcinoma and to find clinical and biologic criteria. A total of 106 patients with HCC seen during the last 3 >ears were analyzed retrospectively for survival in relation to prognostic factors, treatment and disease stage. The results obtained were summarized as follow; 1) HCC vas found frequently in men, and male and female ratio vas 2.5:1. The average age was 55 years in both sex. 2) A prognostic study hased on 91 untreated patients was undertaken. The median survival times according to age, sex, performance status, positive serologic determination of hepatitis B, alcohol intake, alpha-fetoprotein, transaminase, BUN, creatinine values at the time of cliagnosis were statistically not significant. Whereas in all series of patients, bilirubin, tumor size, ascites, serum albumin values were statistically significant. A new staging scheme based on above factors was used. Clearly, thc prognosis depended on clisease stage. The midian survival of stage I patients who receivecl no specific treatment ivas 4.1 months and stage II patients was 1.8 M, and stage III patients svas 0.9 M (p<0.001). 3) The median survival of 91 patients who received no specific treatment was 2.1 M, however 2.3 M for the group of patients(8) placed on systemic chemotherapy, and 9.9 M of the group of patients(7) for hepatic resection. 1) The major causes of death were hepatic failure; 37%(40/106), gastrointestinal bleeding; 28 % (30/106), cachexia; 14% (14/106), respiratory failuire due to lung metastasis; 8% (8/106), intraperitoneal rupture; 3%(3/106). Unclear; 11% (11/106).

K562 세포의 방사선 감수성 변화에 영향을 미치는 신호전달인자

양광모(Kwang Mo Yang),윤선민(Seon-Min Youn),정수진(Soo-Jin Jeong),장지연(Ji-Yeon Jang),조월순(Wol-Soon Jo),도창호(Chang-Ho Do),유여진(Yeo-Jin Yoo),신영철(Young-Cheol Shin),이형식(Hyung Sik Lee),허원주(Won Joo Hur),임영진(Young-Jin Li 대한방사선종양학회 2003 Radiation Oncology Journal Vol.21 No.3

Purpose The human chronic myelogenous leukemia cell line, K562, expresses the chimeric bcr-abl oncopro-tein, whose deregulated protein tyrosine kinase activity antagonizes the induction of apoptosis via DNA damaging agents. Previous experiments have shown that nanomolar concentrations of herbimycin A (HMA) coupled with X-irradiation have a synergistic effect in inducing apoptosis in the Ph-positive K562 leukemia cell line, but genistein, a PTK inhibitor, is non selective for the radiation-induced apoptosis of p210bcr/abl protected K562 cells. In these experiments, the cytoplasmic signal transduction pathways, the induction of a number of transcription factors and the differential gene expression in this model were investigated. Materials and Methods K562 cells in the exponential growth phase were used in this study. The cells were irradiated with 0.5-12 Gy, using a 6 MeV Linac(Clinac 1800, Varian, USA). Immediately after irradiation, the cells were treated with 0.25 M o f H MA and 25 M of genistein, and the expressions and the activities of ablkinase, MAPK family, NF-B, c-fos, c-myc, and thymidine kinase1 (TK1) were examined. The differential gene expressions induced by PTK inhibitors were also investigated. Results: The modulating effects of herbimycin A and genistein on the radiosensitivity of K562 cells were not related to the bcr-ablkinase activity. The signaling responses through the MAPK family of proteins, were not involved either. In association with the radiation-induced apoptosis, which is accelerated by HMA, the expre-ssion of c-myc was increased. The combined treatment of genistein, with irradiation, enhanced NF-Bactivity and the TK1 expression and activity. Conclusion: The effects of HMA and genistein on the radiosensitivity of the K562 cells were not related to the bcr-abl kinase activity. In this study, another signaling pathway, besides the MAPK family responses to radiation to K562 cells, was found. Further evaluation using this model will provide valuable information for the optional radiosensitization or radioprotection. 목 적: 만성 골수성 백혈병 세포인 K562 세포주는 방사선 및 다양한 항암제에 대한 apoptosis에 저항성을 가진다. 지난 연구에서 K562 세포는 방사선에 대하여 내성반응을 보이며, 세포내 PTK의 작용을 억제하고 자방사선조사와 함께 투여한 herbimycin A (HMA)에 의하여 방사선에 대한 apoptosis와 같은 감수성 반응이 유도되는 반면, genistein에 의하여 방사선에 대한 apoptosis 반응이 저해 됨을 확인하였다.본 연구에서는 타이로신 인산화 효소억제에 의한 K562세포의 방사선 반응변화를 조절하는 신호전달경로를 조사하였다. 대상 및 방법 : K562 세포를 지수증식기의 세포 들만 선택하여 실험에 이용하였다. 방사선조사는 6 MeV 선형가속기 (Clinac 1800C, Varian)를 이용하여 200˜300 cGy/min 선량률로 0.5˜12 Gy를 균일하게 조사하였다. HMA와 genis-tein은 각각 0.25μM, 25μM을 방사선 조사 후 즉시 투여하였다. 실험에서 신호전달 경로로 abl kinase, MAPK family, NF-κB, c-fos, c-myc, thymidine kinase1 (TK1)등에서의 단백질 또는 유전자 발현 및 활성을 조사하였다. 또한 약제 투여에 따른 유전자 발현차이(differential gene expression)를 조사하였다. 결 과: Abl kinase의 발현 및 활성 변화를 조사하였으나 PTK 저해제에 의한 방사선 유도 세포사의 변화와의 연관성을 찾을 수 없었다. 세포생존 및 사멸의 신호전달 체계에서 주요조절과 정인 MAPKfamily의 관여 여부확인에서 방사선으로 인한 SAPK/JNK의 활성화의 유도가 관찰되었으나, PTK 저해제에 따른 변화는 없었으며,또한 MAPK/ERK와 p38MAPK활성은 모든 조건에서 변함없이 일정하였다.전사인자 활성화에 대한 조사에서 방사선 조사와 함께 genistein을 투여한 경우에NF-κB 활성이 증가하였다. 유전자 발현 차이의 조사에서 genistein 투여에 의한 TK1유전자 발현 및 단백질 활성이 증가하였다. 결 론: PTK 억제에 의한 K562 세포의 방사선에 대한 반응 변화는 bcr-abl kinase활성과는 무관하게 진행되며, MAPK family경로 외의 다른 경로를 통한 전사인자 활성화 과정이 연관되어 있음을 확인하였다.

잔유 총수담관 결석에 대한 담도내시경 직시하 Electrohydaulic Lithotripsy (EHL) 1예

김진도,강대환,양웅석,조몽,도창호,이동완,허윤,문한규 대한소화기내시경학회 1990 Clinical Endoscopy Vol.10 No.2

Electrohydraulic lithotripsy (EHL) is a method used to breakup the stone by electric discharge in the presence of liquid medium. Recently we experienced a case of successful common bile duct stone removal after EHL during choledochoscopy via T-tube tract, which was the first case of EHL in biliary tract stone in Korea. A 65-year-old female patient was admitted to our hospital because of generalized pruritus after cholcystectomy with T-tube insertion. Obtained cholangiogram showed retained CBD stone which was implssible to remove by Dormian basket and tto dissolute by monooctanoin. After only one session of EHL, we can disintergrat CBD stone into several small pieces and removal of stone was easily done by Basket. After Small cut endoscopic sphincterotomy (EST) remained distal impacted stone was freely passed and control choledochoscopy and cholangiogram showed no remained stone. We think that EHL may be play a potential role in removal of biliary tract stones as in urinary tract stones.

폐결핵과 동반된 결핵성 두개골 골수염 1 예

이민기,신영기,박순규,허재영,도창호,조군제,임홍섭,문원국,최장락,이헌직 대한내과학회 1990 대한내과학회지 Vol.39 No.2

Tuberculous osteitis of the skull is an uncommon condition, first reported by Reid of Erlangen, who described two cases in 1842. By 1933. only 220 cases had been recorded in world literature (Strauss). It occurs in approximately 1%. of all patients with tuberculous osteitis and is diagnosed by clinical manifestation, skull roentgenogram, smear and culture of aspirated pus, and biopsy of granulation material. It responds readily to chemotherapy and the prognosis is favorable. To the best of our knowledge, the condition has not been previously reported in Korea. But recently we experienced a case of tuberculous osteitis of the skull in an 18-year-old male patient who complained of dyspnea, chest pain, multiple swelling of scalp and right eye proptosis. The patient was treated with antituberculous medication and discharged with his condition improved.