AOT 역미셀을 이용한 α-chymotrypsin의 가용화에 대한 연구

노선균(Seon-Gyun Rho),강춘형(Choon-Hyoung Kang) 한국생물공학회 2000 KSBB Journal Vol.15 No.6

다양한 pH 용액에서 네자리 Schiff base 착물이 수식된 유리질탄소 전극의 산소환원 촉매적 특성

노선균 ( Seon Gyun Rho ),한신 ( Shin Han ) 조선대학교 공학기술연구원 2014 공학기술논문지 Vol.7 No.3

The electrocatalytic effect of oxygen reduction was investigated at glassy carbon electrode coated with tetradentate Schiff base transition metal(II) complexes ; Co(II)(SOPD), Co(II)2(SMPD)2 and Co(II)2(SPPD)2 in the 1 M KOH solution. The reduction current of oxygen measured by cyclic voltammetry was increased by about 150 % more than that of bare glassy carbon electrode and the reduction potential was shifted about 140 mV to the positive direction. The reduction current of oxygen was increased by raising scan rate and reduction potential was shifted to the negative potential direction. The electrocatalytic effect of oxygen reduction was investigated in various pH solutions by cyclic voltammetry at the glassy carbon electrode coated with tetradentate Schiff base transition metal(II) complexes. The reduction current of oxygen at glassy carbon electrode coated with Co(II)(SOPD) was increased by about 200 % more than that of bare glassy carbon electrode and the reduction potential was shifted about 300~350 mV to the positive direction, compared to those of bare glassy carbon electrode in the pH 4, 7, and 10 solution. In the pH 10 solution, especially, the reduction current of oxygen at the electrode coated with Co(II)2(SMPD)2 complex was increased by about 200 % and peak potential was shifted about 500 mV to the positive direction.

AOT 역미셀 용액으로부터 BSA의 역추출에 영향을 미치는 인자들에 관한 연구

노선균(Seon-Gyun Rho),강춘형(Choon-Hyoung Kang) 한국생물공학회 2000 KSBB Journal Vol.15 No.6

일정압력하에서 1-propanol/benzene 계의 기-액 상평형

노선균 ( Seon-gyun Rho ),강춘형 ( Choon-hyoung Kang ) 한국화학공학회 2018 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.56 No.2

석유화학산업의 대표적인 물질로 쓰이는 벤젠은 각종 화학 제품의 기초 물질이다. 그러나 일반적으로 벤젠은 석유화학 산업에서 순수 물질로 존재하지 못하고 알코올류와 벤젠 혼합물로 존재하게 된다. 또한 알코올을 한 성분으로 하는 혼합물은 공비 혼합물이 생성되기 때문에 분리 정제를 위해서는 상평형 데이터가 필수적이다. 본 연구에서는 알코올을 대표하는 1-프로판올을 사용하여 1-프로판올/벤젠 계에 대하여 재순환 평형장치를 이용하여 평형 온도/압력 효과에 따른 상평형 연구를 수행하였다. 측정된 기-액 평형 데이터는 UNIQUAC 식과 WILSON 식을 이용하여 상관관계 시키고 Gibbs/Duhem식을 이용하여 열역학적 건전성을 확인하였다. 상평형 실험 결과 RMSE (Root Mean Square Error)와 AAD (Average Absolute Deviation)는 두 모델식에서 0.05 이하의 값을 보여 실험값과 계산 값이 잘 일치함을 알 수 있었다. 또한 Gibbs/Duhem식을 이용하여 열역학적 건전성을 판별한 결과 잔류항 값이 ±0.2 이내에 분포하는 것을 통해 데이터에 대한 건전성을 확인할 수 있었다. Benzene is one of the most widely used basic materials in the petrochemical industry. Generally, benzene exists as a mixture with alcohols rather than as a pure substance. Further, the alcohols-added mixtures usually exhibit an azeotropic composition. In this context, knowledge of the phase equilibrium behavior of the mixture is essential for its separation and purification. In this study, the vapor-liquid equilibrium data were measured in favor of a recirculating VLE apparatus under constant pressure for the 1 - propanol / benzene system. The measured vapor - liquid equilibrium data were also correlated by using the UNIQUAC and WILSON models and the thermodynamic consistency test based on the Gibbs/Duhem equation was followed. The results of the phase equilibrium experiment revealed RMSEs (Root Mean Square Error) and AADs (Average Absolute Deviation) of less than 0.05 for both models, indicating a good agreement between the experimental value and the calculated value. The results of the thermodynamic consistency test also confirmed through the residual term within ± 0.2.

Dextran/DMSO/초임계 CO₂계의 상거동 측정

노선균 ( Seon-gyun Rho ),강동육 ( Dong-yuk Kang ),강춘형 ( Choon-hyoung Kang ) 한국화학공학회 2017 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.55 No.2

최근 약물전달시스템으로 널리 주목받고 있는 dextran의 미립자는 초임계 반용매 공정을 통해 얻을 수 있다. 초임계 반용매(SAS) 공정에서는 DMSO (dimethyl sulfoxide)에 용해되어 있는 dextran이 반용매인 초임계 CO₂의 첨가에 의한 재결정으로 얻어진다. 본 연구에서는 이 공정의 적절한 운전조건을 제시하기 위하여 가변부피 셀을 이용하여 cloud point를 측정함으로써 Dexran/DMSO/CO₂의 상거동을 관찰하였다 실험결과로부터 dextran 미립자 제조를 위한 초임계 반용매 공정의 적절한 온도(300.15 K~330.15 K), 압력(90 bar~130 bar), 용질의 농도(5 mg/ml~20 mg/ml)의 범위를 결정 하였다. Micron-sized dextran particles, which now attract wide attention as a promising drug delivery systems, can be prepared via the supercritical anti-solvent (SAS) process. In SAS process, dextran particles are obtained as a result of recrystallization of dissolved dextran in dimethyl sulfoxide (DMSO) on addition of supercritical CO₂ as an anti-solvent. In this work, with an intention to provide information on the feasible operating conditions of the process, the phase behavior of Dexran/DMSO/CO₂ is observed by measuring the cloud point in favor of a variable volume cell. From the experimental study, it is concluded that a feasible operating condition of the SAS process for preparation of dextran particles would be 300.15 K~330.15 K and 90 bar~130 bar, respectively, and solute concentration ranges from 5mg/ml to 20 mg/ml.

Phenyl Porphyrin 착물들이 수식된 유리질 탄소 전극에서 산소 환원에 대한 촉매 효과

노선균 ( Seon Gyun Rho ),한신 ( Shin Han ),박경희 ( Kyung Hee Park ) 조선대학교 공학기술연구원 2013 공학기술논문지 Vol.6 No.4

The electrocatalytic effect of oxygen reduction was investigated at glassy carbon electrode coated with metal(II) phenyl porphyrin complexes ; Co(II)(TMPP), Fe(II)(TMPP), Co(II)(TSPP), Fe(II)(TOAPP) in the 1 M KOH solution. The reduction current of oxygen measured by cyclic voltammetry was increased by about 200 % more than that of bare glassy carbon electrode and the reduction potential was shifted about 50 mV to the positive direction at glassy carbon electrode modified with metal(II) phenyl porphyrin complexes. The reduction current of oxygen was increased by raising scan rate and reduction potential was shifted to the negative potential direction. Also these reactions were proceed to diffusion controlled. According to raising temperature, the reduction current was increased and the reduction potential was shifted to the positive potential evaluated by determining cyclic voltammetry. The activation energy calculated by Arrhenius plot was 2.26 Kcal/mole at the catalyst modified electrode coated with Co(II)(TMPP) and 1.76 Kcal/mole at the catalyst modified electrode coated with Fe(II)(TOAPP) which was less than that of at the bare glassy carbon electrode, 3.27 kcal/mole. As a result, we can conclude that Co(II)(TMPP) and Fe(II)(TOAPP) complexes behaved as catalyst in the reductin reaction of oxygen.

초임계 유체를 이용한 약물전달 Dextran 입자의 제조

노선균 ( Seon Gyun Rho ),강춘형 ( Cboon Hyoung Kang ) 조선대학교 공학기술연구원 2011 공학기술논문지 Vol.4 No.2

Recently, producing particles via supercritical fluid method is increasing rapidly. This method require very less energy consumption and it is a convenient method for producing the nano and micro scale particles. Usually, this method help to control the particle size of Dextran which is widely u ed as drug delivery substances (DOS) along with supercritical antisolvent (SAS) system. In this study, we determined the effects of temperature, pressure. injection velocity flow and concentration. Our study concludes that this method requires temperature range from 35°C to 50°C, particle size from 0.39 μm to 0.54 μm and pressure about 90 bar to 130 bar. It was also observed that inclination of particle size increases when the concentration increase while the injection velocity now rate (11.6 ml/min) remain same.

상 접촉법을 이용한 BSA의 AOT 역미셀으로 가용화

노선균(Seon-Gyun Rho),강춘형(Choon-Hyoung Kang) 한국생물공학회 2001 KSBB Journal Vol.16 No.1

감압하에서의 Glycerol - Water 의 기액평형

노선균,강춘형 ( Seon Gyun Rho,Choon Hyoung Kang ) 한국화학공학회 1995 화학공학의이론과응용 Vol.1 No.2

Glycerol - 물 계에 대한 등압 기액평형의 측정과 상관관계

노선균,강춘형 ( Seon Gyun Rho,Choon Hyoung Kang ) 한국공업화학회 1997 공업화학 Vol.8 No.6

기상 재순환 평형조를 사용하여 대기압 이하의 여러 압력에서 glycerol-물 계에 대한 기-액 평형을 측정하였다. 이 연구에서 고려한 이성분계는 비등점 차이가 매우 큰 성분들로 구성되어 있다. 비등점의 차이가 큰 혼합물의 경우, 순수한 성분으로부터 유추할 수 있는 물리적 특성은 실제 혼합물의 특성과는 매우 다르므로 정확한 혼합물의 특성을 이해하지 않으면 안된다. 특히 비등점이 높은 성분의 액상농도가 증가하면 혼합물의 비등점이 급격하게 높아지는 현상이 일어난다. 그러므로 실제공정의 효율적 운전을 위해서는 비이상성 혼합물의 상평형에 관한 정보가 필수적이다. 측정한 기-액 평형 데이터는 UNIQUAC 모델을 이용하여 정확하게 상관관계 시킬 수 있었다. 또한, 데이터의 건전성을 확인하기 위하여 열역학적 건전성 테스트를 수행하였다. In this study, vapor-liquid equilibria of a binary system, which consists of glycerol and water, are measured using a vapor-recirculating modified Othmer still at various subatmospheric pressures. The constituent components of the binary system considered in this study exhibit a large difference in the boiling temperatures. Since it is generally observed that the properties of a mixture greatly differ from those of the pure components, the phase equilibrium characteristics of a mixture can not be predicted from the properties of the pure components. Furthermore, an abrupt increase in the boiling temperature occurs as the concentration of the higher boiling component exceeds a certain value. Therefore, it is essential to acquire realistic phase equilibrium data of the mixture for industrial applications. Using the UNIQUAC model, the experimental vapor-liquid equilibrium data are correlated with good accuracy. The thermodynamic consistency test is also performed to ensure soundness of the data.