http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Microarray 분석을 이용한 유채 종자성숙단계별 유전자 발현 양상
노경희 ( Kyung Hee Roh ),박종석 ( Jong Sug Park ),김종범 ( Jong Bum Kim ),김현욱 ( Hyun Uk Kim ),이경렬 ( Kyeong Ryeol Lee ),김순희 ( Sun Hee Kim ) 한국응용생명화학회(구 한국농화학회) 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.4
We observed that oil began to accumulate at 25 seed days after flowering (DAF) and reached the maximum potential at 35 seed DAF of oilseed rape, and the greatest weight of 100 seeds was obtained at 35 seed DAF. To survey a broad analysis of gene expression in developing embryos of Brassica napus, the Bn 300k microarray have been constructed. The Bn 300k Microarrary was designed from 80,696 unigenes clustered from 543,448 ESTs and 780 cDNA at NCBI. These arrays have been hybridized in a series of experiments with probes derived from seeds and leaf of B. napus. Approximately 8.5% of the 7,000 genes were expressed as ratios 2-fold higher in seed (25 DAF) than leaves and 0.4% at ratios 10. Also we observed that storage and cell differentiationrelated genes were highly expressed at 10 DAF, whereas energyrelated genes including fatty acid metabolism were increased up depending on seed maturation using Microarray, which was confirmed by reverse transcriptase polymerase chain reaction. These results suggest that B. napus arrays provide a very useful data set of seed-specific expression that can be further analyzed by examination of the promoter regions of these genes and help our understanding of the complex regulatory network in developing seeds.
노경희 ( Kyung Hee Roh ),박종석 ( Jong Sug Park ),강한철 ( Han Chul Kang ),김종범 ( Jong Bum Kim ),장영석 ( Young Suk Jang ),김광수 ( Kwang Soo Kim ),이한길 ( Hankuil Yi ) 한국응용생명화학회 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.4
유채는 월동작물로 내한성이 약해 남부지역에서만 재배가 가능하다. 따라서 재배면적 확대 및 안정적 생산성 확보를 위해 내한성 증진 품종 육성이 절실하다. 본 연구에서는 유채의 내한성을 증진시키기 위해 보리에서 유래한 HvIRIP 유전자를 CaMV35S 프로모터 조절하에서 전신발현되도록 운반체를 제작 하였고, 아그로박테리움을 이용하여 유채에 형질전환하였다. Southern 분석을 통해 HvIRIP 유전자가 유채 Genome 안으로 전이 되었음을 확인하였다. 또한 Northern 분석을 통해 4℃에서 2주간 처리된 유묘에서 HvIRIP 유전자의 발현이 크게 증대되는 것을 알 수 있었다. 본엽 3-4매 전개 유채 형질전환체를 영하 5℃에서 2일간 저온에 노출한 후 회복여부를 조사한 결과, 대조구는 회복하지 못하고 죽은 반면, 형질전환체는 회복이 정상적으로 이루어졌다. 또한 저온스트레스가 진행되는 동안에 스트레스를 극복하는데 필요한 Proline 함량이 형질전환체에서 크게 증대되는 것이 관찰되었다. 이 외에도 저온스트레스 과정 중에 생성되는 활성산소의 독성을 감소시키는 항산화제 효소인 CAT, SOD 그리고 ADH 활성을 측정한 결과, 대조구에 비해 형질전환체에서 그 함량이 현저히 증가됨을 알 수 있었다. 따라서 이러한 결과를 통해 HvIRIP 유전자가 함유된 유채 형질전환체의 내한성이 증진되었음을 확인하였다. Rapeseed (Brassica napus) is now the second largest oilseed crop after soybean. Cold temperature tolerance is an important agronomic trait in winter rapeseed that determines the plant`s ability to control below freezing temperatures. To improve cold tolerance of rapeseed plants, an expression vector containing an Barley Ice recrystallization inhibition protein (HvIRIP) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into rapeseed plants. Transgenic expression of HvIRIP was proved by southern- and northern-blot analyses. The level of freezing tolerance of transgenic T3 plants was found to be significantly greater than that of wild-type rapeseed plants by freezing assay. Proline accumulation during cold stress was also highly induced in the transgenic rapeseed plants. The transgenic plants exhibited considerable tolerance against oxidative damage induced by cold stress. Our results indicated that heterologous HvIRIP expression in transgenic rapeseed plants may induce several oxidative-stress responsive genes to protect from cold stress.
노경희 ( Kyung Hee Roh ),강한철 ( Han Chul Kang ),김종범 ( Jong Bum Kim ),김현욱 ( Hyun Uk Kim ),이경렬 ( Kyung Ryeol Lee ),김순희 ( Sun Hee Kim ) 한국응용생명화학회 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.3
RNA 결합 단백질들이 유전자 조절의 다양한 범위에 작용한다는 사실이 아주 중요하다. 유전자의 전사에 관련된 유전자 조절이 많이 연구가 되었어도 RNA 의 조절에 관한 연구는 상대적으로 부진한 편이다. RNA 결합 단백질들은 RNA 와 관련되는 각종 과정, 예를 들면 전사, pre-mRNA splicing, polyadenylation, 수송,위치화, 번역, 분해 및 구조의 유지 등 다양한 범위에서 작용을 하고 있다. RNA 결합 단백질들의 많은 부분들이 아직 잘 알려지지 않고 있으며 유전자 발현에 대해 더 잘 이해하기 위해 이러한 부분의 연구가 더 수행되어야 한다. 최근에 유전학, 생화학, 및 유전자들의 생물정보학의 발달 등으로 인하여, RNA 결합 단백질들의 다양한 분야들이 알려지고 있으며 이러한 부분들이 많은 관심을 받고 있다. The role of RNA-binding proteins (RBPs) to regulate expression of genes seems to be very important. RBPs play important roles in RNA related bioprocess such as transcription, pre-mRNA splicing, polyadenylation, transport, localization, translation, turn over and maintenance of structure. Despite of many researches on RNA binding proteins, detailed mechanisms of these proteins have not been fully understood. It seems that many parts of RBPs remains unknown and should be characterized for the better understanding of gene expression. Recently, genetic, biochemical, and bioinformatic analysis of genomes revealed a vast array of RBPs and many parts are interesting to understand bioprocessing including gene expression.
노경희 ( Kyung Hee Roh ),곽보경 ( Bo Kyoung Kwak ),김현욱 ( Hyun Uk Kim ),이경렬 ( Kyeong Ryeol Lee ),김순희 ( Sun Hee Kim ),서미정 ( Mi Chung Suh ),김효진 ( Hyo Jin Kim ),김종범 ( Jong Bum Kim ) 한국응용생명화학회 2011 Journal of Applied Biological Chemistry (J. Appl. Vol.54 No.1
To improve genetic transformation of Brassica napus winter cultivar ``Youngsan``, factors influencing shoot regeneration and transformation from cotyledonary petioles were investigated. Shoot induction was enhanced in the combination of 0.5 mg/L NAA and 2~4 mg/L kinetin. Silver nitrate was essential for successful shoot regeneration, ranging from 5 to 9 mg/L. The addition of GA3 promoted plant regeneration. Among the tested Agrobacterium strains, co-cultivation times, and antibiotic selection regimes, choice of appropriate Agrobacterium strain was the most critical factor for efficient transformation of B. napus cv. ``Youngsan``. The EHA105 succinamopine strain was the most efficient and the maximum transformation efficiency was 26.8%. Transgenic shoots were selected on 10 mg/L phosphinothricin (PPT) containing media. The transgenic plants expressing bar and gus genes were resistant for commercial herbicide Basta and stained with X-Gluc. Southern blot hybridization indicated that the presence of one to three gus gene copies per genome and inheritance of the gus gene into the T1 generation.
고구마 정단분열조직으로부터 체세포배발생 및 식물체 재분화에 미치는 casein의 영향
신공식,노경희,이연희,박용환,서석철,Shin, Kong-Sik,Roh, Kyung-Hee,Lee, Yeon-Hee,Park, Young-Whan,Suh, Seok-Cheol 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1
고구마의 정단배양에 의한 배발생 캘러스로부터 대량증식 체계가 개발되어져 왔다. 고구마 정단분열조직은 1mg/L 2,4-D가 첨가된 MS배지에서 배양 4주 경에 최적의 배발생 캘러스가 형성되었다. 또한 2,4-D가 첨가된 배지에 casein을 첨가함으로써 고구마 신천미 품종의 배발생 효율을 최고 90%이상으로 2.4-D단독처리보다 현저하게 증가시켰다. 배발생 캘러스로부터 체세포배의 유도는 식물생장조절제가 제거된 MS 기본배지에서 효과적으로 형성되었으며 300∼500mg/L casein을 첨가한 배지에서는 더 높은 형성 빈도와 녹색의 단단한 체세포배가 발달하였다. 한편, 2mm이하의 체세포배로부터 이차 배발생 캘러스 형성 및 체세포배의 발달이 100∼300mg/L casein의 첨가에 의해 증가하였다 배발생 캘러스에서 얻어진 체세포배는 직접 MS기본배지에서 쉽게 각 기관이 형성되었으며, 발근과 shoot를 발달시켜 정상적인 식물체로 하여 토양에 성공적으로 옮겨 심을 수 있었다. An efficient protocol has been developed for rapid mass propagation of sweetpotato from shoot-tips derived embryogenic callus. Optimal embryogenic callus was induced from shoot apical meristem explants on Murashige and Skoog (MS) medium supplemented with 1mg/L 2,4-D. The addition of casein hydrolysate in the media increased the embryogenesis efficiency of sweetpotato. Somatic embryos were easily induced from the embryogenic callus on MS basal medium containing 300-500mg/L casein hydrolysate without phytohormon. Treatment of casein hydrolysate (100∼300mg/L) with 1mg/L 2,4-D also improved the secondary embryonic efficiency from somatic embryos below 2mm in length. Plant regeneration was achieved via somatic embryogenesis and direct organogenesis. Regenerated planlets with well developed shoots and roots on MS basal medium were successfully transferred to soil.
김순희 ( Sun Hee Kim ),강한철 ( Han Chul Kang ),노경희 ( Kyung Hee Roh ),김현욱 ( Hyun Uk Kim ),이경렬 ( Kyeong Ryeol Lee ),김원용 ( Won Yong Kim ),박종화 ( Jong Hwa Park ),정인식 ( In Sig Chung ),김종범 ( Jong Bum Kim ) 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.2
Most of pharmaceutical proteins are usually produced by the animal, insect cell and yeast culture. The proteins must be produced in strictly controlled-facilities, which is one of major causes for increasing production cost. So, in spite of increasing demand on the proteins in worldwide, their generalization has been very restrictive. To decrease the production cost, many scientists have been interested in production of pharmaceutical proteins by using the plant system for a long time. The plant system must be alternative production means, for it is easily scalable, more production cost effective, and safer than animal system. For this purpose, several techniques have been developed: stable expression by transformation of nuclear and chloroplast genome and transient expression by viral vectors. Among them, the deconstructed viral vector system has made it possible to produce recombinant proteins massively, rapidly, and transiently. So, the plant viral expression systems might be a suitable platform for production of pharmaceutical proteins in plants. In this review, we will describe the plant viral vector systems and their application.
한범수,정영재,노경희,박종석,조강진,김용환,김종범,Hahn Bum-Soo,Jeong Young-Jae,Roh Kyung-Hee,Park Jong-Sug,Cho Kang-Jin,Kim Yong-Hwan,Kim Jong-Bum 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.4
Plants have considerable advantages for the production of antigenic proteins because they provide an inexpensive source of protein and an easy administration of vaccine. Since a publication describing edible plant vaccine of HBsAg in 1992, a number of laboratories around the world have studied the use of plants as the bioreactor to produce antigenic proteins of human or animal pathogens. Over the last ten years, these works have been mainly focused on three major strategies for the production of antigenic proteins in plants: stable genetic transformation of either the nuclear or plastid genome, or transient expression in plants using viral vectors. As many antigenic proteins have been expressed in tobacco, also several laboratories have succeeded to express genes encoding antigenic proteins in other crop plants: potato, tomato, maize, carrot, soybean and spinach. At present many works for the production of edible plant vaccine against bacteria-mediated diseases have mostly performed the studies of enterotoxins and adhesion proteins. Also the development of new-type antigens (pili, flagella, surface protein, other enterotoxin and exotoxin etc.) is required for various targets and more efficacy to immunize against microorganism pathogens. Many works mostly studied in experimental animals had good results, and phase I clinical trial of LTB clearly indicated its immunogenic ability. On the other hand, edible plant vaccines have still problems remained to be solved. In addition to the accumulation of sufficient antigen in plants, human health, environment and agriculture regulation should be proven. Also oral tolerance, the physiological response to food antigens and commensal flora is the induction of a state of specific immunological unresponsiveness, needs to be addressed before plant-derived vaccine becomes a therapeutic option.